バイオ実験キット Dr.ジーンシリーズ 取扱説明書 第 10 版 株式会社ニッポンジーン 和光純薬工業株式会社 目次 ページ 1.本キットについて ······································································· 4 2.キット内容の確認 キット構成 ········································································ ·· 5 試薬の組成 ········································································ ·· 6 ·························································· ·· 7 キット以外に必要な物 3.使用上の注意 ·········································································· ·· 8 4.DNA、制限酵素、およびアガロース電気泳動について DNA の構造 制限酵素 ······································································· ·· 9 ··········································································· 10 ······················································· ·11 アガロースゲル電気泳動 5.実験準備 氷の準備 ··········································································· 13 37℃の水浴の準備 ······························································· 13 アガロースゲル電気泳動用試薬の準備 1×TAE の調製 ···························································· 14 ··················································· 15 アガロースゲルの作製 Stains-All 溶解液の調製 6.各班の試薬等のチェック ················································· 18 ··························································· 20 7.実験プロトコル 実験の流れ 実験操作 ········································································ 21 ··········································································· 22 8.データ解析 質問例 ·············································································· 31 解答例 ·············································································· 34 9.関連製品 ··············································································· 38 3 「ニッポンジーン」は商標登録されています 製品についてのお問い合わせ先 和光純薬工業株式会社 フリーダイヤル: 0120-052-099 フリーファックス: 0120-052-806 E-mail: [email protected] ホームページ: http://www.wako-chem.co.jp 株式会社ニッポンジーン 研究試薬部 学術営業課 TEL: 076-451-6548 FAX: 076-451-6547 E-mail: [email protected] ホームページ: http://www.nippongene.com 《A051102F090902》 44
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