20151023 «{³µ ÐÉ]Ã^7PV>T; ^ Ôм^Ã^À¸3Ø° mjy{DQT?I8VMC6Jnbz³µáITbMâ!i Áá+( $1â ¥§ zá%2 âaxq ¢¤á (+âaWANG Chenguangá24 44â³µo[ ¿fá ,âË pYá$+( +â-! ³µAVO"n¬Í3kÇg/ÐÉ]Ã^7PV>T; ÔÃ^À¸3Ø°( \`!bz!e3Ç/G797PV>T;"¬v!«{³µ G797PV>T;Ä!° ¡³µ£!W yÞ3h'!"2014!FVLRg{Î Õ#0 ÐÉ]ßÖ!W/STEDßÖSTEDßÖ"!Ã^ßÖ!Û¯3yXs/ à¶Ùbɾ ,0(Ìcݹ½` /r|! Ó+@TH:Ï!e !È~3k¾ (SV=V^!©3Æ/-@TH:Ï ºlÃ^À¸!ÅÀ¨¹½3Ç/Q7J7PV>T;!}ѱG797PV >T;&!®Ú(0( ITbM!³µAVO"Ã^À¸bzC-Naphox3Ø°!À¸!Ã^À¸3" / Xs/¼^3*3- STED ßÖ ,/».ÒȦ (Zs!° ,.!À¸"tÝ *dSTEDßÖ3}®SLR X/)!w²Ä 3´·( ³µ"E7Bg{×Ê5T<GTCUKNVuܪ!9TQ7Tª 0( 1 1023 _Ø çí&ùÁ"n Þgóõ0ÞÜČ8|Ćx4N<?<U[DYB'Ü&Þêçí8º4å³ā" ¬Ĕ%â ,a!/ÿm<U[DYB'æã&ÚĎ4±0w8ÿm" ®¢1Éo!4.ćČ8Þ,,ć4"!,&.V<Q<U[ DYB&Â/Ãu$²Ñ" ß25 , "6&ÿm<U[DYB%'üã$Ë×,m8Éo²Î" ©Ę!'m&µ%1 k+0ğ·b&ÚĎ\%Ć è%ćÖ!4ČÚ&&^ĜħîěpĈûĨ%rðÞ ß4ÿmģ ,.ģ©Ę!Í ,,ÚÝ÷>WYEIZ :GS 19 `ï%¸:GS&£%14"ćÖ|û$k&'m&Òę& 2 p& 1 , !! 3400700 nm (nm '1 m & 10 lp& 1) &Òę8/|Ćm8ß}%'200 nm Ýċ]&Ĝá""%$3,5% òún0=<WEDNAFYOAĎ$# Þê÷ý8¶Þgóõ0p&' 200 nm e^&! 3ÿm<U[DYB!' Ħ¿%ć4"'Ġ!©ò$Þgóõ0ÞÚÓp&w8 3&,,!ć4¾Ñ &Ěâ'ÞÚçí&²Ñ%ġ½8/2ėĄĊĢ! 3&çí÷ÕÄ , 9$ac21 10 t&200 nm &8đ4"&!4½$ÿmģ©Ęđ Ĉkÿmģ©Ęâ¿5,1994 % Stephan E. Hell z(J<HZTGAERVYAçí±) 2 %1 Ěâ5ĉ»o´r (stimulated emission depletion; STED) ģ©Ę'&\!STED ģ©Ę!'ÿmþñ8ô}ħVSWxĨČÚ%mħvĐmĨ"58J[KHÛ%{39 STED m& 2 ìĤ&X[C[8Ù,ÿmþñ%vĐm8Ù2">LW@[Û¯&ĥv ĐÛ¯%$3ÿm8â$2§%>LW@[&f¡Û¯%°3,2%ÿmþñ&ÿ mÊÒę13/ęÒę& STED m83%¦ 4"%13&Ėp&ÿmþñ&-%ĉ»oÜ Č8¤Đ¥rã% STED m"~Òę!&-âm,&Òę&m&-8P;WF[ !{3ĝ"!STED m&¦2$aªĖ&-&ÿm8ĥ®¢%Éo4"!41%$ 4.¼y nm ë¢%,!îěpĈû8ĥ.4"!,&â¿8äq3%¨Ç&ÿmģ ©Ę!'k+0 ą4"!$òún8Ħ¿%ć!4"Ì"é5 ,N<?<U[DYB%$â8/2àÅã$³ā! 32014 &M[SWxč' Hell z8.đĈkģ©Ę8â¿ 3 &çí÷%ď25, 52&đĈkģ©Ę8Ðßã$²Ñ" ßã%ß4.%'iØ" $ ,&Â/$ÿmþñ&øm!đĈkÿmģ©Ę!'ē&ÿmģ ©Ę"Ï) ÈÎ%¥X[C[m&Ù8«Ą"4"2ÿmþñ&ăþÔs$ Ģ"$ ,h(STED <U[DYB% $4"¿2%5 îěpĈû' STED m&¥¢$4*#ĥ ,STED m8¥4"!~À%ÿmþñ&ăþ/jĕ5 ,,Ü&ømÿmþñ&dĂÈ! 4 Alexa Fluor® 488 0 ATTO 488 "þñ! 2 STED <U[DYB%ßĞ&măþ'Ôs! 3ö3ĒÖ8Ā"Ġ! 4& ÜÛ!5!'STED ģ©Ę&¥-! 4ĥîěpĈû8Þ,,V<Q<U[DYB 8Ā"!,9$7đĈkģ©Ę³ā&ÆÇ&Ãß8yp%â¹4.%'¥ u$X[C[m&Ù%/ø4½$ÿmþñ&Ěâ«Ą_|Ë! 2 đ. C-Naphox çoåÙ" STED Ċă%47>V^E]AÊl 8% dÑÖAZ^R'²&ÃÙďCĐ×ÙďNĐĀÙďOĐx;b"7s ȵ%S?ÙďBĐY]ďPĐB>ÙďSiĐÒĎďSĐ$#&û!' .6É58$nÙ;Û /ø0"sLC>];2"%¨$çoåÙ&ąÌ%y6Û<!.&b!15 ©n Ù! 7Y] (P) ;0ºços&¸ü"çoÅ&Ïć% Ùx!¹¸ü;2 C-Naphox ·1 C-Naphox 'Ƭ2áo%m8çoåÙ" ÷ ô+7þÕ% Y]"à čáo;2";Ìí. Ð587 Alexa Fluor® 488 3 ATTO 488 "½ 2kÎ%čáo;Ó.i(v$@GO]X]R (300 W) ;É nm &o;Ä7ċ;è"92 «Ć&oÄ%4 460 11 Alexa Fluor® 488 " ATTO 488 88t¯Â& 26.2% 4) 96.7%.!sï&%C-Naphox ' 99.9% sï7"$ ¼ .}±f! 12 «ĆÄ;è"9ATTO 488 58.7%.!sï&% C-Naphox 't¯Â& 99.5%",-āÎ%ȼ !ÑÖJ^U'C-Naphox ;É 7"s6. ÈÚâ;´åSTED Ċă;Éço>V^ E]A*&É;ò/.&ܳ·1 STED o&Ä`! 50 Þ6úî;è 2 83% &t¯ço;j¤!7":6.}±f! Alexa 488 ;É|%' §&Þ6úÁ!,-q%ìå .""ÄÎ$ܳ!$:C-Naphox &i Î%čáo%46²a{ã! 7"ß58 Þ6ú STED >V^E]At1 Æ&! ."1"d&® Y];Ă"7sñð%46öáoçoåÙ C-Naphox &ąÌ%w&åÙÈÚâ&Þ 6ú STED >V^E]A% 2,"<#ìå$";ª5%.&öáoço åÙ&Í%46Ą«Ć&Þ6úÁ;gI>UXRF STED >V^E]A3X>Q STED > V^E]A"²&çoåÙ!'a{ã! öïlço>V^E]AÆ!7"ß 58.dąÌçoåÙ;ùÌ&1.öïlĊă¢é"Û/|:7"! §&ÈÆõ;čØÚ%>V^E]A!7¡¿&ąÌ%$72&"¯8. °ÑÖ³&_ÿ'Ô¢é¥äº¸ďJSTĐ ËÎuüÑÖ¦ýc¶J^U ÑÖďCRESTĐ R\ GF>]KA[^DW]%~čºãNO¸üh&urÑÖĈďp¾ »À ÑÖÝ£Đ% 7ÑÖóĉHPM π TKY=Z&uë"ºãÌÆďÑÖeêàĒzæĐ&_Ç" 3 è:8. ¹àÝá ×Z2HL8K7ü½¼ ´åù:K@6åú×ZÓÉ0Êl+]-×Z0Û- ª ß ] (stimulated emission)üdæµ Ã MÄ,[Zm«î*"S¾0(Z Z(i~)^~Z)³ ] æ,)![Z Ú-# %0 ]+wµÂ-¶ã[ ]0RwµÂ-ëÃùѲ ]ú(u-ß ©V-'õ[Z0-Ѳ ]± clß ]0æ- çÝX×Z÷íü×Z÷í í i~$!^~T3>J5Lµ (wµ) Â-ë ·âñºù200 nm yú0ç -øÆð^ÝÐ0(×Z÷ ÌÁß ]a (STED; stimulated emission depletion) ÷í PgM ×Z^~ ¤¯¶ ã 0 _ ¹ Z f u ÷ í (PALM; Photoactivated localization microscopy $ ! STORM; stochastic optical reconstruction microscopy) -bÍ0ï» Stephan W. Hell hzÍ ÇÍWilliam E. Moerner hz *" Erig Betzig hz ï» ûn2014?LCJfä N. Z tüt!« êØ¢o yùò´úu-xl«y -`ìövt,&¶ã0Z!ôÀ« MÄ,« ÏMÙ åÈ~ å0/ (' ´åZ0-tæ-ZêØ¢o -t*-Z Üú!Z ,ùt «îW«îî-#}- èóÞâ¡nÕÍ èóÞü Angewandte Chemie International Edition â¡nü A Phosphole Oxide Based Fluorescent Dye with Exceptional Resistance to Photobleaching: A Practical Tool for Continuous Imaging in STED Microscopy ùV{¼ÎZ0(×ZÓÉüSTED ÷í*-éË2HL8K7 ' ¹¼ ;LJ C-Naphoxú Õ Íü Chenguang Wang, Aiko Fukazawa, Masayasu Taki, Yoshikatsu Sato, Tetsuya Higashiyama, Shigehiro Yamaguchi ùChenguang Wang, ®°~|s¨¬UÖÒe§qOjÔú â¡\ï£ü201510¥23£ DOI: 10.1002/anie.201507939 (http://dx.doi.org/10.1002/anie.201507939) ¦Qrl/Y nk}=IK9A4LF<1B¸p^~¿Å (ITbM) ELGDL8: http://www.itbm.nagoya-u.ac.jp j Ô TEL: 052-789-2291 E-mail: [email protected] ELGDL8 (¿Å): http://orgreact.chem.nagoya-u.ac.jp/Home.html 4 %# TEL: 052-747-6404 E-mail: [email protected] (() ): http://www.higashiyama-lab.com/ " ITbM !/ ', * TEL: +81-52-789-4999 FAX: +81-52-789-3053 E-mail: [email protected] "+.$&TEL: +81-52-789-2699 FAX: +81-52-788-6272 E-mail: [email protected] 5
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