顕微操作で卵・初期胚の性質を調べる

us
s
m
as am u h
as am u sh i
as am u sh i a
s
i
a
s
sa m us hi as am
m us hi as am u
m us hi as am u sh
us h a a
s
i
u
h
i
s
m
hi a a
s
i
us h
s
m
as am u h i
s
ᐇ⩦ࣉࣟࢢ࣒ࣛ㛤Ⓨ 6
⛬ᗘࡢ෌⌧ᛶ࡛ᚓࡽࢀࡿࠋᏛ⏕ࡀ⮬ࡽࡢ
㢧ᚤ᧯స࡛༸࣭ึᮇ⬇ࡢᛶ㉁
ࢆㄪ࡭ࡿ
ᡭ࡛ࠕᐇ㦂ࠖࡋࠊᐇ㦂⤖ᯝ࡜ࡋ࡚ᚓࡽࢀ
⨾⃰ᕝᣅဢ
ࡋࠊࡑࡢ␗ᖖᛶࡢࠕศᯒࠖࢆࡶ࡜࡟ṇᖖ
ࡿ␗ᖖ࡞⬇ࡢᙧែࢆ⮬ࡽࡢ┠࡛ࠕほᐹࠖ
Ⓨ⏕࣓࢝ࢽࢬ࣒ࢆࠕ⪃ᐹࠖࡍࡿࡇ࡜ࡣࠊ
⏕≀⣔Ꮫ㒊࡟࠾ࡅࡿⓎ⏕Ꮫᇶ♏ᩍ⫱ࣉࣟ
Key words: 㢧ᚤ᧯సࠊ࢘ࢽࠊ༸ࠊ⬇ࠊ
micromanipulation
ࢢ࣒ࣛ࡜ࡋ࡚ࣂࣛࣥࢫࡢྲྀࢀࡓࣉࣟࢢࣛ
࣒࡜࠸࠼ࡼ࠺ࠋ
⏕≀Ꮫࢆᑓᨷࡋ࡞࠸Ꮫ⏕ࡸࠊᩍဨ㣴ᡂ
⣔Ꮫ㒊ࡢᏛ⏕࡟࡜ࡗ࡚ࡣࠊ⬇᧯సᐇ㦂ࡣ
1. ࡣ ࡌ ࡵ ࡟
㧗ᰯ⏕≀ࡢᩍ⛉᭩࡛㥆ᰁࡳ῝࠸⣲ᮦ࡛࠶
༸ࡸึᮇ⬇ࢆ㢧ᚤ㙾ୖ࡛ᡭ⾡ࡍࡿࠕ㢧
ࡿࡓࡵ࡟⯆࿡ࢆࡦࡁࡸࡍ࠸ࠋ㧗ᰯࡢᩍ⛉
ᚤ᧯స micromanipulationࠖࡣࠊ༸࠾ࡼ
᭩࡛ࡣࠊᮍཷ⢭༸ࡢ≉ᐃࡢ㡿ᇦ࡟ᒁᅾࡍ
ࡧึᮇ⬇ࡢ㡿ᇦ≉␗ⓗ࡞ᶵ⬟ࢆᐇ㦂ⓗ࡟
ࡿ⣽⬊㉁ỴᐃᅉᏊࡢ㔜せᛶࢆ♧ࡍ౛࡜ࡋ
ゎᯒࡍࡿ᪉ἲࡢ୍ࡘ࡛࠶ࡿࠋ㢧ᚤ᧯సἲ
࡚ࠊࢩࣙ࢘ࢪࣙ࢘ࣂ࢚ࡢᴟ⣽⬊㉁ࡢ⛣᳜
࡟ ࡼ ࡿ Ⓨ ⏕ ◊ ✲ ࡢ Ṕ ྐ ࡣ ྂ ࡃ
ᐇ㦂࡟୪ࢇ࡛ࠊ࢘ࢽᮍཷ⢭༸ࡢ༸∦Ⓨ⏕
㸦Horstadius 1973㸧ࠊ㧗ᰯࡢ⏕≀ᩍ⛉᭩
ᐇ㦂ࡶྲྀࡾୖࡆࡽࢀ࡚࠸ࡿࠋึᮇⓎ⏕࡟
࡛ࡶࡉࡲࡊࡲ࡞ື≀ࢆᑐ㇟࡜ࡋࡓྂ඾ⓗ
࠾ࡅࡿ⣽⬊㛫┦஫స⏝ࡢᙺ๭ࢆㄝ᫂ࡍࡿ
ᐇ㦂ࡀྲྀࡾୖࡆࡽࢀ࡚࠾ࡾࠊᏛ⏕࡟ࡶ㥆
ᐇ㦂౛࡜ࡋ࡚ࡣࠊ୧⏕㢮ࡢ༙⬇సᡂᐇ㦂
ᰁࡳ῝࠸ෆᐜ࡛࠶ࡿࠋ㢧ᚤ㙾ࡢୖ࡛ࡢ᧯
ࡸࢩ࣮࣐࣮ࣗ࣌ࣥ࢜࢞ࢼ࢖ࢨ࣮⛣᳜ᐇ㦂
సࡣ୍ぢ㞴ࡋࡑ࠺࡟ឤࡌࡽࢀࡿ࠿ࡶࡋࢀ
࡜࡜ࡶ࡟ࠊ࢘ࢽࡢ༙⬇సᡂᐇ㦂࡜ᑠ๭⌫
࡞࠸ࡀࠊ㐺ษ࡞஦๓‽ഛࢆࡍࢀࡤㄡ࡛ࡶ
⛣᳜ᐇ㦂ࡶ㢖ฟ࡛࠶ࡿࠋ
ᐇ㦂ྍ⬟࡛࠶ࡿࠋᮏ✏ࡢ┠ⓗࡣࠊ⮫ᾏᐇ
ᮏ✏࡛⤂௓ࡍࡿᐇ㦂ᢏ⾡ࡣࠊ㠀⏕≀⣔
⩦࡛㢧ᚤ᧯సᢏ⾡ࢆẼ㍍࡟฼⏝࡛ࡁࡿࡼ
ࡢᏛ⏕࡛࠶ࡗ࡚ࡶẚ㍑ⓗ▷᫬㛫࡛ᢏ⾡ࢆ
࠺ࠊ⡆⣲࡞ᐇ㦂ᢏ⾡ࢆ㛤Ⓨࡋࠊࡑࢀࡽࢆ
⩦ᚓ࡛ࡁࡿ࡛ࣞ࣋ࣝ࠶ࡿࠋࡲࡓࠊᮏ✏࡛
ࢃ࠿ࡾࡸࡍࡃゎㄝࡍࡿࡇ࡜࡟࠶ࡿࠋ
ゝཬࡍࡿࡍ࡭࡚ࡢჾලࠊ⸆ရࡣࢭࣥࢱ࣮
⬇᧯సᐇ㦂ࡣ⏕≀⣔Ꮫ㒊Ꮫ⏕ྥࡅࡢࣉ
ࣟࢢ࣒ࣛ࡜ࡋ࡚ࣂࣛࣥࢫࡢྲྀࢀࡓᐇ⩦࣓
࡟ᐇ⩦⏝࡜ࡋ࡚‽ഛࡋ࡚࠶ࡾࠊࢭࣥࢱ࣮
฼⏝⪅ࡣࡍ࡭࡚౑⏝ྍ⬟࡛࠶ࡿࠋ
ࢽ࣮ࣗ࡜࠸࠼ࡿࠋࡇࡢࢱ࢖ࣉࡢᐇ㦂ࡢ≉
ᚩࡣࠊᐇ㦂⤖ᯝࡀ⏕ࡁࡓື≀ࡢᙧ࡜ࡋ࡚
⌧ࢀࡿⅬ࡟࠶ࡿࠋ༸࣭⬇ࢆࠊ୍ᐃࡢ࣮ࣝ
2. ᮦ ᩱ ࡜ ᪉ ἲ
ࣝ࡟ࡋࡓࡀࡗ࡚ᡭ⾡ࡍࡿ࡜ࠊ࠶ࡿඹ㏻ࡋ
㸦 2A㸧 ඲ ᐇ 㦂 ࡟ ᚲ せ ࡞ ᮦ ᩱ ࣭ ჾ ල
ࡓ≉ᚩࢆࡶࡘ␗ᖖ࡞ᙧែࡢಶయࡀࠊ࠶ࡿ
⬇᧯సᐇ㦂࡟ඹ㏻ࡋ࡚ᚲせ࡞ჾල࣭ᮦ

Download Report