研究室の実績
・学会発表
1. 熊谷祐也, Weeranuch Lang, 貞廣樹里, 奥山正幸, 森
春英, 木村淳夫: Gluconobacter
oxydans 由来 dextran dextrinase による直鎖イソマルトメガロ糖の酵素合成. 日本応用糖
質科学会平成 25 年度大会ポスター賞(2013 年 9 月, 鹿児島).
2. Song KM, Kobayashi K, Okuyama M, Mori H, Kimura A: Production and characterization of
recombinant -glucosidase from Podospora anserina which reveals high regioselectivity for
-1,3-glucosidic linkage. 植物・海草由来多糖類国際会議ポスター賞第１位 (2012 年 7
月, ナント, フランス).
3. Tagami T, Okuyama M, Mori H, Kimura A: Structural element determining the diverse
specificities for chain-length of substrate in glycoside hydrolase family 31 -glucosidases. 第
９回糖質生物工学国際会議ポスター賞第１位 (2011 年 5 月, リスボン, ポルトガル).
4. Ngiwsara L, Iwai G, Mori H, Okuyama M, Kimura A: Putative role of Tyr227, Gln349 and
Leu350 near catalytic amino acids of honeybee -glucosidase III. 4th Symposium on the
Alpha-Amylase Family ポスター賞第３位 (2010 年 9 月, スモーレニス, スロバキア).
・論文発表
1. Maneesan J, Matsuura H, Tagami T, Mori H, Kimura A: Production of 1,5-anhydro-D-fructose
by an α-glucosidase belonging to glycoside hydrolase family 31. Biosci Biotechnol Biochem
in press, 2014.
2. Okuyama M, Yoshida T, Hondoh H, Mori H, Yao M, Kimura A: Catalytic role of the calcium
ion in GH97 inverting glycoside hydrolase. FEBS Lett 588:3213-3217, 2014.
3. Lang W, Kumagai Y, Sadahiro J, Maneesan J, Okuyama M, Mori H, Sakairi N, Kimura A:
Different molecular complexity of linear isomaltomegalosaccharides and β-cyclodextrin for
enhancing a solubility of azo ethyl red: towards the dye biodegradation. Bioresour Technol
169:518-524, 2014.
4. Suzuki N, Fujimoto Z, Kim YM, Momma M, Kishine N, Suzuki R, Suzuki S, Kitamura S,
Kobayashi M, Kimura A, Funane K: Structural elucidation of the cyclization mechanism of
α-1,6-glucan by Bacillus circulans T3040 cycloisomaltooligosaccharide glucanotransferase. J
Biol Chem 289: 12040-12051, 2014.
5. Funane K, Ichinose H, Araki M, Suzuki R, Kimura K, Fujimoto Z, Kobayashi M, Kimura A:
Evidence for cycloisomaltooligosaccharide production from starch by Bacillus circulans T-3040.
Appl Microbiol Biotechnol 98:3947-3954, 2014.
6. Lang W, Sirisansaneeyakul S, Martins LO, Ngiwsara L, Sakairi N, Pathom-aree W, Okuyama
M, Mori H, Kimura A: Biodecolourisation of food colorant azo dye by deep sea Dermacoccus
from the Mariana Trench. J Environ Manage 132:155-164 2014.
7. Saburi W, Kobayashi M, Mori H, Okuyama M, Kimura A: Replacement of the catalytic
nucleophile aspartyl residue of dextran glucosidase by cysteinesulfinate enhances
transglycosylation activity. J Biol Chem, 288: 31670-31677, 2013.
8. Song KM, Okuyama M, Kobayashi K, Tagami T, Mori H, Kimura A: Characterization of a
glycoside hydrolase family 31 α-glucosidase involved in starch utilization in Podospora
anserina. Biosci Biotechnol Biochem 77: 2117-2124, 2013.
9. Lang W, Sirisansaneeyakul S, Ngiwsara L, Mendes S, Martins LO, Okuyama M, Kimura A:
Characterization of a new oxygen-insensitive azoreductase from Brevibacillus laterosporus
TISTR1911 toward dye decolorization using packed-bed metal affinity reactor. Bioresour
Technol 150:298-306, 2013.
10. Tagami T, Yamashita K, Okuyama M, Mori H, Yao M, Kimura A: Molecular basis for the
recognition of long-chain substrates by plant -glucosidase. J Biol Chem 288: 19296-19303,
2013.
11. Tankrathok A, Iglesias-Fern´andez J, Luang S, Robinson CR, Kimura A, Rovira R, Hrmova
M, Cairns JK: Structural analysis and insights into glycon specificity of the rice GH1
-D-mannosidase. Acta Crystallogr D 69:2124-2135, 2013.
12. Tagami T, Okuyama M, Nakai H, Kim YM, Mori H, Taguchi K, Svensson B, Kimura A: Key
aromatic residue, possibly presenting at subsite +2 and +3, of glycoside hydrolase family 31
-glucosidase regulates recognition on long-chain substrates. Biochim Biophys Acta
1834:329-335, 2013.
13. Suzuki N, Kim YM, Momma M, Fujimoto Z, Kobayashi M, Kimura A, Funane K:
Crystallization
and
preliminary
X-ray
crystallographic
analyses
of
cycloisomaltooligosaccharide glucanotransferase from Bacillus circulans T-3040. Acta
Crystallogr F 69:946-949, 2013.
14. Shinoki A, Lang W, Thawornkuno C, Kang HK, Kumagai Y, Okuyama M, Mori H, Kimura
A, Ishizuka S, Hara H: A novel mechanism for promotion of quercetin glycoside absorption
by isomaltomegalosaccharide (-1,6-glucosaccharide having more than 10 glucosyl units) in
rat small intestine. Food Chemistry 136:293-296, 2013.
15. Song KM, Nishimura M, Tagami T, Okuyama M, Mori H, Kimura A: Aromatic residue on
β->α loop 1 in the catalytic domain is important to transglycosylation specificity of glycoside
hydrolase family 31 α-glucosidase. Biosci Biotechnol Biochem 77:1759-1765, 2013.
17. Tagami T, Tanaka Y, Okuyama M, Mori H, Kimura A: Enzymatic synthesis of
acarviosyl-maltooligosaccharides using disproportionating enzyme 1. Biosci Biotechnol
Biochem 77:312-319, 2013.
18. Iwaya H, Lee JS, Yamagishi S, Shinoki A, Lang W, Kang HK, Okuyama M, Mori H, Hara H,
Kimura A, Ishizuka S: Degree of polymerization in dietary -1,6-gluco-saccharides modulates
symptom of experimental colitis in rats. Plos One 7:e50658, 2012.
19. Suzuki R, Terasawa K, Kimura K, Fujimoto Z, Momma M, Kobayashi M, Kimura A, Funane
K: Biochemical characterization of a cycloisomaltooligosaccharide glucanotransferase from
Paenibacillus sp. 598K. Biochim Biophys Acta 1824:919-924, 2012.
21. Kim YM, Saburi W, Yu S, Nakai H, Maneesan J, Kang MS, Chiba S, Kim D, Okuyama M,
Mori H, Kimura A: -Glucosidase-catalyzed novel reaction on 1,5-anhydrofructose,
suggesting new metabolic pathway for production of glucose from starch. J Biol Chem 287:
22441-22444 2012.
22. Suzuki N, Kim YM, Fujimoto Z, Momma M, Okuyama M, Mori H, Funane K, Kimura A:
Structural elucidation of dextran degradation mechanism by Streptococcus mutans dextranase
belonging to glycoside hydrolase family 66. J Biol Chem 287: 19916-19926, 2012.
23. Kim YM, Kiso Y, Muraki T, Kang MS, Nakai H, Saburi W, Lang W, Kang HK, Okuyama M,
Mori H, Suzuki R, Funane K, Suzuki N, Momma M, Fujimoto Z, Oguma T, Kobayashi M,
Kim D, Kimura A: Novel dextranase catalyzing cycylodextran-formation: Identification of
catalytic amino acids and their functions newly revealed by chemical rescue reactions. J Biol
Chem 287: 19927-19935, 2012.
24. Kim YM, Yamamoto E, Kang MS, Nakai H, Saburi W, Okuyama M, Mori H, Funane K, Kim
D, Kimura A: Bacteroides thetaiotaomicron VPI-5482 glycoside hydrolase family 66
homolog catalyzes dextranolytic and cyclization reactions. FEBS J, 279: 3185-3191, 2012.
25. Ngiwsara L, Iwai G, Tagami T, Sato N, Nakai H, Okuyama M, Mori H, Kimura A: Amino
acids in conserved region II are crucial for substrate specificity and regioselectivity in
transglucosylation of honeybee GH-13 -glucosidases. Biosci Biotechnol Biochem
76:1967-1974, 2012.
26. Suzuki N, Kim YM, Fujimoto Z, Momma M, Kang HK, Funane K, Okuyama M, Mori H,
Kimura A: Crystallization and preliminary crystallographic analysis of dextranase from
Streptococcus mutans. Acta Crystallogr F 67: 1542–1544, 2011.
27. Kobayashi M, Hondoh H, Mori H, Saburi W, Okuyama M, Kimura A: Calcium ion-dependent
increase in thermostability of dextran glucosidase from Streptococcus mutans. Biosci Biotechnol
Biochem 75:1557-1563, 2011.
28. Kim YM, Shimizu R, Nakai H, Mori H, Okuyama M, Kang MS, Fujimoto Z, Funane K, Kim
D, Kimura A: Truncation of N- and C-terminal regions of Streptococcus mutans dextranase
enhances catalytic activity. Appl Microbiol Biotechnol 91: 329-339, 2011.
29. Kang HK, Kimura A, Kim D: Bioengineered glucansucrase of Leuconostoc mesenteroides for
preferred oligosaccharide synthesis using sucrose. J Agric Food Chem 59: 4148-4155, 2011.
30. Funane K, Kawabata Y, Kim YM, Kang HK, Fujimoto Z, Kimura A, Kobayashi M: Function
of C-terminal region of cycloisomaltooligosaccharide glucanotransferase from Bacillus
circulans T-3040. Biochim Biophys Acta 1814: 428-434, 2011.
31. Kim YM, Kimura A, Kim D: Novel quantitative method for the degree of branching in
dextran. Food Sci Biotechnol 20: 537-541, 2011.
32. Kang HK, Kim YM, Nakai H, Kang MS, Hakamada W, Okuyama M, Mori H, Nishio T, Kimura
A: Suicide substrate-based inactivation of dextranase by -epoxyalkyl -D-glucosides. J Appl
Glycosci 57:111-117, 2010.
33. Opassiri R, Maneesan J, Akiyama T, Pomthong B, Jin S, Kimura A, Ketudat-Cairns JR: Rice
Os4BGlu12 is a wound-induced β-glucosidase that hydrolyzes cell-wall-β-glucan-derived
oligosaccharides and glycosides. Plant Sci 179:273-280, 2010.
・著書
1. 木村淳夫: 糖質酵素の分子機構. 応用糖質科学 4:3-16, 2014.
2. Kimura A: Isomaltomegalosaccharide. Agriculture Materials (issued by Seoul National
University), pp. 25-35, 2011.
・受賞
1. 木村淳夫：糖質酵素の分子機構に関する研究. 日本応用糖質科学会学会賞 (2013 年 9
月, 鹿児島).
2. Kyung-Mo Song：真菌類由来-グルコシダーゼの構造と機能に関する研究. 2013 年第
11 回（平成 25 年度）日本農芸化学会北海道支部学生会員奨励賞（2013 年 11 月, 札幌).
3. 田上貴祥：-グルコシダーゼの長鎖マルトオリゴ糖特異性に関与する構造因子に関す
る研究. 2012 年度日本応用糖質科学会北海道支部奨励賞（2013 年 2 月, 札幌).
4. 奥山正幸：α-グリコシダーゼの機能と構造に関する研究. 2010 年度日本農芸化学会
奨励賞（2010 年 3 月, 東京).
5. Kang Min-Sun：Molecular mechanism and application of bacterial glycosidases（細菌由来
糖質分解酵素の分子機構および応用に関する研究). 平成 21 年度北海道大学大塚賞制
度に基づく受賞者（大塚賞） (2010 年 3 月, 札幌).
6. Kang Min-Sun：ヒト大腸に生息する微生物の糖質酵素に関する分子解析. 平成 21 年度
（第７回）日本農芸化学会北海道支部学生会員奨励賞 (2009 年 7 月, 札幌).