e24 Islet Capture Microplate XF24・XF 24 XF24/XFe24⏝ ⮅ᓥ䜻䝱䝥䝏䝱䝬䜲䜽䝻䝥䝺䞊䝖 ⣽⬊እࣇࣛࢵࢡࢫࢼࣛࢨ࣮XF24・XFe24ࢆ⏝࠸ࠊ ⮅ᓥࡢ࣑ࢺࢥࣥࢻࣜᶵ⬟ࢆࣜࣝࢱ࣒ィ ࡍࡿࡇࡀྍ⬟㸟 ⮅ᓥ⣽⬊ࡢࣜࣝࢱ࣒࣭ࣂ࢚࢜ࢼࢪ࢙ࢸࢡࢫࢆホ౯ 㧗⢭ᗘࢹ࣮ࢱࢆᚓࡿࡓࡵࡢ᪂࡞ࢹࢨࣥ Innovative design for break-through data XF24/XFe24⏝⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺࡣࠊ≉ࠊ ⮅ᓥࡢ⣽⬊௦ㅰࢆホ౯ࡍࡿⅭタィࡉࢀࡓ≉ู࡞ࣉ࣮ࣞࢺ ࡛ࡍࠋྜ≀ࡢᣑᩓࢆ㜼ᐖࡏࡎࠊࡲࡓࠊᐇ㦂ࡢయࢆ㏻ࡌ ࡚⣽⬊ࢆᖖಖࡘⅭᚲせ࡞ᰤ㣴ศ࣭࢞ࢫࡀ⮬⏤ ฟ᮶ࡿ≧ែࢆ⥔ᣢࡋ࡞ࡀࡽࠊ ᐃࡍࡿ⮅ᓥࢆࠊXF24/XFe 24⨨ࡢࢭࣥࢧ࣮ࡽ㐺ษ࡞㊥㞳ಖࡘࡀ࡛ࡁࡲࡍࠋ 䝥䝻䞊䝤䞉⮅ᓥ䜻䝱䝥䝏䝱䜴䜵䝹᩿㠃ᅗ 䝠䝖⮅ᓥ䛾䜾䝹䝁䞊䝇䜈䛾ᛂ 800 㻞㻜䡉㻹䜾䝹䝁䞊䝇㻌 Ⅼ 䝁䞁䝖䝻䞊䝹㻔䜾䝹䝁䞊䝇↓㻕㻌 700 OCR (pMoles/min.) Assay real-time bioenergetics of islets cells Seahorse Bioscience♫ࡣࠊ࣎ࢫࢺࣥᏛ་Ꮫ㒊࠶ࡿࠊ ࣑ࢺࢥࣥࢻࣜARC(Advancing Research through Collaborations)ඹྠࡋࠊin vitro࡛↓യࡢ⮅ᓥࣂ࢚࢜ࢼ ࢪ࢙ࢸࢡࢫࢆホ౯ࡍࡿࡓࡵࠊ XF24/XFe24⏝⮅ᓥ࢟ ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺࢆ᥇⏝ࡋࡓࠊ᪂ࡋ࠸ࣉࣟࢺࢥ ࣝࢆ㛤Ⓨࡋࡲࡋࡓࠋ ࡇࡢࣉࣟࢺࢥࣝࡼࡾࠊ⢾ᒀࡘ࠸࡚◊✲ࢆ⾜ࡗ࡚࠸ ࡿ⛉Ꮫ⪅ࡀᚲせࡍࡿࠊጞཎ(ཎⓎᛶ)⮅ᓥࡢࡼ࠺࡞ࠊࡼࡾ ⏕⌮ⓗ࡞⣽⬊ࢆ⏝࠸ࡓࢵࢭࡀྍ⬟࡞ࡾࡲࡋࡓࠋ XF・XFeࣇࣛࢵࢡࢫࢼࣛࢨ࣮ࢆ⏝࠸ࡓࢵࢭࡣࠊ ࢡ࣮ࣛࢡ㟁ᴟࢆ⏝࠸ࡿᚑ᮶ᡭἲẚࠊࣁࢫ࣮ࣝࣉࢵࢺ (1ᅇࡢࢵࢭ࡛20ࢧࣥࣉࣝࡢฎ⌮ࡀྍ⬟)࡛࠶ࡾࠊ⏝ ࡍࡿ⮅ᓥ⣽⬊ࡀࡼࡾᑡ࡞ࡃ࡚῭ࡴ(1࢙࢘ࣝ࠶ࡓࡾ30ಶ⛬ ᗘ)࠸࠺Ⅼࡶ࠶ࡾࡲࡍࠋ 600 500 400 300 200 12 ࢢࣝࢥ࣮ࢫ 24 36 ࢜ࣜࢦ࣐ࢩࣥ 48 60 72 84 96 108 120 㛫ศ 䝤䝗䜴⢾ὀධ䛻䜘䜚䚸⮅ᓥ䛾㓟⣲ᾘ㈝㏿ᗘ䠷㻻㻯㻾䠹䛜䚸 ᇶ♏྾㏿ᗘ䜢㉸䛘䛶ୖ᪼䛧䚸䜎䛯䚸䛣䛾⌧㇟䛿䚸 䜲䞁䝇䝸䞁ศἪ䛻┦㛵䛩䜛䛣䛸䛜♧䛥䜜䜎䛧䛯䚹 ⢾ᒀ⮅ᓥ䛷䛿䚸䝤䝗䜴⢾䜈䛾ᛂ䛜㕌䛔䛸䛥䜜䛶䛔䜎䛩䚹 䚷 㻭㼀㻼ྜᡂ㓝⣲㜼ᐖ㻙䜸䝸䝂䝬䜲䝅䞁䜢㏣ຍⓗ䛻ຍ䛩䜛 䛣䛸䛻䜘䜚䚸⮅ᓥ䛾䝭䝖䝁䞁䝗䝸䜰ඹᙺຠ⋡䜎䛯䛿㻭㼀㻼ᅇ㌿ ຠ⋡䛜♧䛥䜜䜎䛧䛯䚹 ͤ㠃ࡶࡈぴୗࡉ࠸ࠋ ༟㉺ࡋࡓ౽ᛶࢫ࣮ࣝࣉࢵࢺࢆࡈᥦ౪㸟 Unsurpassed convenience and throughput ᶵ⬟ࡋ࡚࠸ࡿ⮅ᓥ࠾ࡅࡿ㓟⣲ᾘ㈝ࢆṇ☜ ᐃࡍࡿ ࡇࡣࠊࡇࢀࡲ࡛ࡑ࠺⡆༢࡛ࡣ࠶ࡾࡲࡏࢇ࡛ࡋࡓࠋ XF24/XFe24⏝⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺࡣࠊ㛫 ࡢࡿࢥ࣮ࢸࣥࢢࣉࣟࢺࢥ࣭ࣝྲྀᢅ࠸ࡢ㠃ಽ࡞⎔ ὶࢩࢫࢸ࣒ࢆࠊࢩࣥࣉࣝ࡞24࢙࣐࢘ࣝࢡࣟࣉ࣮ࣞࢺ ⨨᭰࠼࡚ࡃࢀࡲࡍࠋ ࣥࢪ࢙ࢡࢩ࣏࣮ࣙࣥࢺ ࢙࣐࢘ࣝࢡࣟࣉ࣮ࣞࢺ ගࣇࣂ࣮ ࣉ࣮ࣟࣈ ͤගࣇࣂ࣮ࣉ࣮ࣟࣈࡣࠊ ࠉ⦪᪉ྥືࡁࡲࡍࠋ Ỉ⣲࢜ࣥ(H+) ࢭࣥࢧ࣮ࢫ࣏ࢵࢺ 㓟⣲(O2)ࢭࣥࢧ࣮ ࢫ࣏ࢵࢺ ࢟ࣕࣉࢳࣕࢫࢡ࣮ࣜࣥ ⮅ᓥ O2 H+ ࢫࢡ࣮ࣜࣥࡢࡾ ͤศࡾ᫆ࡃࡍࡿࡓࡵࠊ᩿㠃ᅗࡢࢫࢣ࣮ࣝࡣྜࡗ࡚࠸ࡲࡏࢇࠋ XF24/XFe24⏝⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺࡣࠊ25ಶ ࡢ࢟ࣕࣉࢳࣕࢫࢡ࣮࣓ࣜࣥࢵࢩࣗᵓ㐀ࡀᒓࡋ࡚࠸ࡲࡍࠋ ࣉ࣮ࣞࢺᗏ㒊ࡣ❑ࡳࡀ࠶ࡾࠊ༢㞳ࡋࡓ⮅ᓥ⣽⬊ࢆྛ࢙࢘ ࣝ50㹼70ಶᑟධࡋࠊᑓ⏝ࡢᤄධࢶ࣮ࣝࢆ⏝࠸࡚ࠊ࢟ࣕ ࣉࢳࣕࢫࢡ࣮ࣜࣥࢆྲྀࡅࡿྠࡌࢶ࣮ࣝࢆ⏝࠸ᐜ᫆㝖ཤ ࡶྍ⬟ࡔࡅࡢ㠀ᖖࢩࣥࣉ࡛ࣝᐜ᫆࡞ᡭ㡰 ࡛ ᐃࢆ㛤 ጞࡍࡿࡇࡀ࡛ࡁࠊ⏕⏘ᛶࢆྥୖࡉࡏࡲࡍࠋ Applications XF24/XFe24⏝⮅ᓥ࢟ࣕࣉ ࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺࡣࠊ ⮅ᓥ⣽⬊௨እࠊ ⬡⫫⣽⬊࡞ࡢᾋ㐟⣽⬊ ࢵࢭࡶ⏝ྍ⬟࡛ࡍࠋ ࢟ࣕࣉࢳࣕࢫࢡ࣮ࣜࣥ㉺ࡋࡢ⮅ᓥ XF24・XFe24 Islet Capture Microplate ࢜ࣥࢹ࣐ࣥࢻ࣭Webinarࡢࡈෆ http://www.seahorsebio.com/learning/webinars/details.php?wID=19 "How to Measure Mitochondrial Functionin Whole Islets" Orian Shirihai, MD PhD, Associate Professor Boston University Medical Center ͤ↓യࡢ࣐࢘ࢫ࣭ࣄࢺࣛࣥࢤࣝࣁࣥࢫ⮅ᓥ࡛ࠊࣂ࢚࢜ࢼࢪ࢙ࢸࢡࢫࢆ ࠉ ᐃࡍࡿ᪉ἲࡘ࠸࡚ཧ⪃ྍ⬟࡞ࠊ45ศ㛫ࡢື⏬㓄ಙ࡛ࡍࠋ ࠉୖグURLࡼࡾࢡࢭࢫࡋࠊ ࠾ᐈᵝሗࢆࡈⓏ㘓㡬ࡃ↓ᩱ࡛ど⫈ྍ⬟࡛ࡍࠋ Whole Islet༢㞳ࡋࡓࢡ࣮ࣟࣥ⮅ȕ⣽⬊࡛ࠊ 㢮ఝࡋࡓࢢࣝࢥ࣮ࢫࡢᛂࡀ☜ㄆࡉࢀࡲࡋࡓࠋ OCR B A 1100 508 990 446 880 385 OCR (pMoles/min.) OCR (pMoles/min.) OCR A 570 323 261 199 770 660 550 440 137 330 76 220 14 110 - 48 B 0 15.8 28.9 42.0 55.1 68.1 81.2 94.3 107.4 120.5 133.5 146.6 㛫 (min.) ࣐࢘ࢫWhole Islet࢙࢘ࣝ࠶ࡓࡾ⮅ᓥ70ಶ⏝ 0 7 14 20 27 34 41 48 54 61 68 75 82 89 96 103 110 117 124 131 138 㛫 (min.) ༢㞳ࢡ࣮ࣟࣥȕ⣽⬊(INS1) 㟷㸸 [A] 20mMࢢࣝࢥ࣮ࢫὀධࠊ[B]࢜ࣜࢦ࣐ࢩࣥ_㉥㸸 [A] ✵ὀධࠊ[B]࢜ࣜࢦ࣐ࢩࣥ ͤWhole Isletsࡢ༢㞳᪉ἲཧ⪃ື⏬㸸 http://www.jove.com/index/Details.stp?ID=255 Ordering Information ەFluxPak-XF24⮅ᓥ࢟ࣕࣉࢳࣕࣔࢹࣝ>ᆺᘧ㸸101174-100] ͤFluxPakࢵࢭ࢟ࢵࢺx6ࠊ࢟ࣕࣜࣈ࣮ࣞࢩࣙࣥ⁐ᾮx1ࠊ ࠉ⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺx6ࠊ࢟ࣕࣉࢳࣕࢫࢡ࣮ࣜࣥx25ࠊᒓ ەXF24/XFe24⏝ ⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺ [ᆺᘧ㸸101122-100] (ධᩘ6/⟽)ࠉ ͤ ࢟ࣕࣉࢳࣕࢫࢡ࣮࣭ࣜࣥᒓ ࠉ ⮅ەᓥ࢟ࣕࣉࢳࣕࣉ࣮ࣞࢺ⏝ ࠉࢫࢡ࣮ࣜࣥᤄධࢶ࣮ࣝ ࠉ>ᆺᘧ㸸101135-100] ͤ ⁛⳦࣭⏝ྍ⬟ ͤXFe24ࣔࢹ࡛ࣝ࠾࠸ࡢሙྜࡣࠊ㏻ᖖࡢXFe24⏝FluxPakࢆࡈ㉎ධ㡬ࡁࠊ⮅ᓥ࢟ࣕࣉࢳ࣐ࣕࢡࣟࣉ࣮ࣞࢺ࣭(ࢫࢡ࣮ࣜࣥᤄධࢶ࣮ࣝ)ࢆ㏣ຍ࡛ࡈ㉎ධୗࡉ࠸ࠋ ࠾ەၥྜࡏඛSeahorse♫᪥ᮏ⥲௦⌮ᗑ㸸 ͤᮏࣃࣥࣇࣞࢵࢺグ㍕ࡢ〇ရࡣࠊࡍ࡚◊✲࣭ᐇ㦂⏝࡛ࡍࠋ ே࣭ື≀ࡢデ᩿࠶ࡿ࠸ࡣ⒪➼ࡢ⮫ᗋ⏝㏵⏝ࡍࡿࡇࡣ࡛ࡁࡲࡏࢇࠋ ᮾி㒔ᩥி༊ᑠ▼ᕝ 1-3-25 ᑠ▼ᕝᅜࣅࣝ9F Phone:03-3816-0851 [email protected] http://www.primetech.co.jp/ rev02 (2014.04)
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