乱用薬物の多種多様化に対応した違法ドラッグの高感度分析法の開発

ᲨʏဇᕤཋƷ‫ٶ‬ᆔ‫ٶ‬ಮ҄ƴ‫ࣖݣ‬Ơƨ
ᢌඥȉȩȃǰƷ᭗ज़ࡇЎௌඥƷ᧏ႆ
٤ᾢဈ⻞৻㧔␹ᄹᎹ⋵ⴡ↢⎇ⓥᚲ㧕
‫ޤ⸒✜ޣ‬
㆑ᴺ࠼࡜࠶ࠣߦࠃࠆ੐ઙ෸߮ஜᐽⵍኂߩᄙ⊒߇ᄢ߈ߥ␠ળ໧㗴ߣߥߞߡ޿ࠆ‫⼊ޕ‬ኤᐡߩ
⊒⴫ߦࠃࠇ߫‫ޔ‬ᐔᚑ ᐕᐲ਄ඨᦼߩ⣕ᴺ࠼࡜࠶ࠣߦଥࠆᬌ᜼ઙᢙ߇೨ᐕหᦼᲧߢᄢ߈ߊჇ
ടߒߡ߅ࠅ ‫ޔߚ߹ޔ‬ᐔᚑ ᐕᐲෘ↢ഭ௛⑼ቇ⎇ⓥߩႎ๔ᦠ ߦࠃࠆߣ‫⑼␹♖ޔ‬ක≮ᣉ⸳
߳ߩㅢ㒮߽ߒߊߪ౉㒮ߒߚਥߚࠆේ࿃⮎‛ߣߒߡ⣕ᴺ࠼࡜࠶ࠣ߇ ⇟⋡ߦᄢ߈ߥഀวࠍභ
߼ߡ޿ࠆߎߣ߇␜ߐࠇߡ޿ࠆ‫ߥ߁ࠃߩߎޕ‬㆑ᴺ࠼࡜࠶ࠣߩ⬧ᑧታᘒ߳ߩኻ╷ߣߒߡ‫ޔ‬ᵹㅢ
ߔࠆ㆑ᴺ࠼࡜࠶ࠣ⵾ຠߩ฽᦭⮎‛ࠍಽᨆߒߡߘߩታᘒࠍ᣿ࠄ߆ߦߔࠆߣߣ߽ߦ‫ޔ‬㆑ᴺ⮎‛
ࠍ฽᦭ߔࠆ⵾ຠߩᵹㅢࠍ㒖ᱛߔࠆᔅⷐ߇޽ࠆ‫ޔߒ߆ߒޕ‬ᬌ಴ߐࠇࠆੂ↪⮎‛ߪᄙ⒳ᄙ᭽ൻ
ߒߡ߅ࠅ‫ޔߚ߹ޔ‬ᓸ㊂ߩᴺⷙ೙⮎‛߇ᬌ಴ߐࠇߚ੐଀߽ႎ๔ߐࠇߡ޿ࠆ‫ޔ߼ߚߩߘޕ‬᳢↪
⊛ߥ 1&5 ࠞ࡜ࡓࠍ↪޿ࠆ *2.% ᴺ෸߮ࠬࠠࡖࡦ᷹ቯߦࠃࠆ .%/5 ᴺߩߺߢߪ‫ޔ‬ಽᨆߩㄦㅦᕈ‫ޔ‬
㘃ૃߒߚ⮎‛ᚑಽߩಽ㔌෸߮ᬌ಴ᗵᐲ߇ਇචಽߣߥࠆ ᔨ߇޽ࠆ‫*ޔ߼ߚߩߘޕ‬2.% ࠞ࡜ࡓ
ߣߒߡࡍࡦ࠲ࡈ࡞ࠝࡠࡈࠚ࠾࡞㧔એਅ‫ޟ‬2(2‫߁޿ߣޠ‬㧕ࠞ࡜ࡓࠍ↪޿ߡ‫ޔ‬2&# ᬌ಴ེࠍធ⛯
ߒߚ⿥㜞ㅦᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯ᴺ㧔એਅ‫ޟ‬72.%2&# ᴺ‫߁޿ߣޠ‬㧕෸߮ 54/㧔5GNGEVGF
4GCEVKQP/QPKVQTKPI㧕ᚻᴺࠍ↪޿ߚᶧ૕ࠢࡠࡑ࠻ࠣ࡜ࡈࠖ࡯㧙࠲ࡦ࠺ࡓ⾰㊂ಽᨆᴺ㧔એਅ
‫ޟ‬.%/5/5 ᴺ‫߁޿ߣޠ‬㧕ߦࠃࠆಽᨆᴺࠍᬌ⸛ߒߚ‫ޕ‬
‫⎇ޣ‬ⓥᣇᴺ‫ޤ‬
㧝 ኻ⽎⮎‛
ㄭᐕߩ⮎੐ᴺᜰቯ⮎‛‫⧎ޔ‬ዥࠄߩႎ๔
෸߮ฦㇺ㆏ᐭ⋵ߩᬌᩏ⚿ᨐ╬ࠍ〯߹߃‫ޔ‬วᚑࠞ
ࡦ࠽ࡆࡁࠗ࠼♽⮎‛߅ࠃ߮ࠞ࠴ࡁࡦ♽⮎‛ࠍਛᔃߦㆬቯߒ‫ޔ‬ಽᨆᴺᬌ⸛ߩኻ⽎⮎‛ߣߒߚ‫ޕ‬
㧞 ⹜⮎෸߮⹜ᶧ
ੂ↪⮎‛ߩᮡḰຠߣߒߡ‫ޔ‬ਥߦ☨࿖ %C[OCP%JGOKECN%QORCP[ ⵾╬ߩ⹜⮎ࠍ૶↪ߒߚ‫ޕ‬
ࠕ࠮࠻࠾࠻࡝࡞ߪ๺శ⚐⮎⵾ *2.% ↪߹ߚߪ .%/5 ↪ࠍ૶↪ߒ‫ߪ࡞࡯ࡁ࠲ࡔ߮ࠃ߅㉄ࠎࠅޔ‬
๺శ⚐⮎⵾․⚖‫ޕߚߒ↪૶ࠍ⵾࠴࠶࡝࠼࡞ࠕࡑࠣࠪߪࡓ࠙࠾ࡕࡦࠕ㉄߉ޔ‬
㧟 ᮡḰṁᶧ╬ߩ⺞⵾
ᮡḰຠࠍㆡቱࡔ࠲ࡁ࡯࡞ߦṁ⸃࡮Ꮧ㉼ߒߡ⺞⵾ߒߚ‫ޕ‬ฦ⮎‛ߩỚᐲࠍ 72.%2&# ᴺߩᬌ
⸛ߢߪ ǴIO. ߣߒ‫ޔ‬.%/5/5 ᴺߢߪ PIO. ߣߒߚ‫ޕ‬54/ ᧦ઙᬌ⸛ߢߪ‫ޔ‬ฦ⮎‛ࡔ
࠲ࡁ࡯࡞ṁᶧࠍ O/ ߉㉄ࠕࡦࡕ࠾࠙ࡓࠕ࠮࠻࠾࠻࡝࡞ᷙᶧ
ࠍ↪޿ߡᏗ㉼ߒ‫⚳ᦨޔ‬
Ớᐲࠍ PIO. ߣߒߚṁᶧࠍ૶↪ߒߚ‫ޔ߅ߥޕ‬ᔅⷐߦᔕߓߡㆡቱỚᐲࠍ⺞ᢛߒߚ‫ޕ‬
㧠 ૶↪ߒߚಽᨆᯏེ߅ࠃ߮ᯏེ᧦ઙ╬
72.%2&# ᴺ
ᯏེ㧦#%37+6;72.%
㧙㧙
ᬌ಴ེ㧦#%37+6;72.%2&#Gǳᬌ಴ེ㧔᷹ቯ▸࿐㧦㨪PO‫࡯࠲࠾ࡕޔ‬ᵄ㐳㧦PO㧕
ࠞ࡜ࡓ㧦#%37+6;72.%*552(2㧔☸ᓘ ǴO‫ޔ‬OOOO‫ޔ‬ᣣᧄ࠙ࠜ࡯࠲࡯࠭㧕
⒖േ⋧㧦# ᶧ㧦㧑ࠅࠎ㉄᳓ṁᶧ‫ޔ‬$ ᶧ㧦ࠕ࠮࠻࠾࠻࡝࡞
ࠣ࡜ࠫࠚࡦ࠻᧦ઙ㧦# ᶧ$ ᶧ
ߢ ಽ㑆଻ᜬߒ‫ߩߘޔ‬ᓟ ಽ㑆ߢ
ߦ⋥✢⊛
ߦᄌൻߐߖߚᓟ‫ ޔ‬ಽ㑆଻ᜬߔࠆ‫ޕ‬
ᵹ㊂㧦O.OKP‫ࡓ࡜ࠞޔ‬᷷ᐲ㧦͠‫ޔ‬ᵈ౉㊂㧦Ǵ.
.%/5/5 ᴺ
ᯏེ3VTCR.%/5/5U[UVGO ߅ࠃ߮ *2*2.%
ࠞ࡜ࡓ5WP5JGNN2(2
☸ᓘ ǴO‫ޔ‬OOOO‫࠭࡯ࠫࡠࡁࠢ࠹ࠢ࠶࠾ࡑࡠࠢޔ‬
⒖േ⋧㧦# ᶧ㧦O/ ߉㉄ࠕࡦࡕ࠾࠙ࡓ‫ޔ‬$ ᶧ㧦ࠕ࠮࠻࠾࠻࡝࡞
ㅍᶧ᧦ઙԘ㧔*+.+%GNWVKQP㧕㧦# ᶧ$ ᶧ㧔㧦㧕ߦߡ ಽ㑆଻ᜬߒ‫ߩߘޔ‬ᓟ ಽ㑆ߢ
‫ ߦࠄߐޔ‬ಽ㑆ߢ㧔㧦㧕ߦ⋥✢⊛ߦᄌൻߐߖߚᓟ‫ ޔ‬ಽ㑆଻ᜬ
ߔࠆ‫ޕ‬㧔ᵹ㊂㧦O.OKP㧕
ㅍᶧ᧦ઙԙ㧔+UQETCVKEGNWVKQP㧦# ᶧ$ ᶧ
ᵹ㊂㧦O.OKP
ࠗࠝࡦൻ㧦ࠛ࡟ࠢ࠻ࡠࠬࡊ࡟࡯ࠗࠝࡦൻᴺ
ࠗࠝࡦൻ᧦ઙ㧦⣕ṁᇦ᷷ᐲ͠‫ޔ‬ශട㔚࿶8‫ޔ‬ᭂᕈ㧦ࡐࠫ࠹ࠖࡉ
54/ ࡄ࡜ࡔ࡯࠲࡯ߩ⸳ቯ෸߮⸃ᨆߦߪ‫ޔ‬#PCN[UV ࠰ࡈ࠻࠙ࠚࠕ
8GTࠍ૶↪ߒߚ‫ޕ‬
㧡 ᮨᡆ⹜ᢱṁᶧߩ⺞⵾
Ꮢ⽼ߩࡂ࡯ࡉ࠹ࠖ ⒳㘃ߩฦ I ߦ‫ ࡞࡯ࡁ࠲ࡔࠇߙࠇߘޔ‬O. ࠍട߃ߡ ಽ㑆⿥㖸ᵄߦ
ࠃࠅ᛽಴ߒ‫ޔ‬㆙ᔃಽ㔌ᓟߩ਄Ẵߺᶧࠍ ୚Ꮧ㉼ߒ‫ࠈ࡯࠲࡞ࠖࡈߦࠄߐޔ‬ㆊߒߚᶧࠍᮨᡆ⹜
ᢱṁᶧߣߒߚ‫ߩߘޕ‬ᮨᡆ⹜ᢱṁᶧߦߟ޿ߡ‫ޔ‬วᚑࠞࡦ࠽ࡆࡁࠗ࠼♽⮎‛ ⒳㘃
,9*‫ޔ‬
:.4‫ޔ‬/#/‫'ޔ‬#/‫ޔ‬#-$0
HNWQTQRGPV[NCPCNQI‫ޔ‬,9* ෸߮ #$(7$+0#%#
෸ ߮ ࠞ ࠴ ࡁ ࡦ ♽ ⮎ ‛ ╬ ⒳ 㘃 RGPVGFTQPG ‫ ޔ‬/&28 ‫ ޔ‬FKOGVJ[NOGVJECVJKPQPG ‫ޔ‬
HNWQTQCORJGVCOKPG‫ޔ‬ǩ282‫ޔ‬ǩ2$2‫̉̉ޔ‬/&2$2 ෸߮ 0GVJ[NDWRJGFTQPGࠍᦨ⚳Ớ
ᐲ߇ 72.%2&# ᴺಽᨆ↪ߣߒߡ ǴIO.‫ޔ‬.%/5/5 ᴺಽᨆ↪ߣߒߡ PIO. ߦߥࠆࠃ߁
ߦߘࠇߙࠇᷝടߒ‫‛⮎ޔ‬ᷝടᮨᡆ⹜ᢱṁᶧߣߒߚ‫ޕ‬
‫⚿ޣ‬ᨐ෸߮⠨ኤ‫ޤ‬
㧝 72.%2&# ᴺߦࠃࠆ 2(2 ࠞ࡜ࡓࠍ↪޿ߚੂ↪⮎‛ߩ㜞ㅦ৻ᢧಽᨆ᧦ઙߩᬌ⸛
㆑ᴺ࠼࡜࠶ࠣ⵾ຠਛߩ⮎‛ࠍ․ቯߔࠆ㓙‫ޔ‬᭴ㅧ⇣ᕈ૕ߩሽ࿷ߪ⇐ᗧߔߴ߈ ᔨ੐㗄ߩ৻
ߟߢ޽ࠆ‫ޕ‬᭴ㅧ⇣ᕈ૕ߪൻቇ⊛ᕈ⾰߇㘃ૃߒߡ޿ࠆ߽ߩߩ‫ߩߘޔ‬ᴺⷙ೙߇⇣ߥࠆ႐ว߽ᄙ
޿‫ޔ߼ߚߩߘޕ‬1&5 ࠞ࡜ࡓએᄖߩ⇣ᕈ૕ಽ㔌ᚻᲑߣߒߡ‫ޔ‬᭴ㅧ⇣ᕈ૕ߩಽ㔌ߦఝࠇࠆ 2(2
ࠞ࡜ࡓࠍ↪޿ߚㅒ⋧ಽ㔌ߦ߅ߌࠆ৻ᢧಽᨆ᧦ઙߩ⏕┙ࠍ⋡ᮡߦᬌ⸛ࠍⴕߞߚ‫ޕ‬
ᬌ಴ེߣߒߡ 2&# ᬌ಴ེࠍ૶↪ߒߚߎߣ߆ࠄ‫ޔ‬⒖േ⋧ߩࠣ࡜ࠫࠚࡦ࠻ㅍᶧ᧦ઙߩᬌ⸛ߢ
ߪ‫࡞࡝࠻࠾࠻࠮ࠕޔ‬෸߮࡝ࡦ㉄᳓ṁᶧߩ૶↪ߦࠃࠅ‫ࠍ࠻ࡈ࡝࠼ߩࡦࠗ࡜ࠬ࡯ࡌޔ‬ᛥ߃‫ޔ‬ච
ಽߥᬌ಴ᗵᐲࠍ⏕଻ߒߚ‫ߩߘޕ‬ㅍᶧࡄ࠲࡯ࡦࠍ⒳‫ޘ‬ᬌ⸛ߒߚ⚿ᨐ‫⦟ޔ‬ᅢߥಽ㔌߇ᓧࠄࠇߚ
ㅍᶧࡄ࠲࡯ࡦߦ߅ߌࠆಽᨆኻ⽎⮎‛ᚑಽߩ଻ᜬᤨ㑆ࠍ⴫ߦ␜ߒߚ‫ޕ‬
㧙㧙
⴫72.%2&#ᴺ෸߮.%/5/5ᴺߦ߅ߌࠆฦ⮎‛ߩࡇ࡯ࠢ଻ᜬᤨ㑆ਗ߮ߦ54/ࡄ࡜ࡔ࡯࠲࡯
LC-MSMS
UPLC
Compound
*
**
Q1
Q3
Dwell DP
Rt.
Rt.㽲 Rt.㽳
indan-2-amine
2.49
15.37
134
91
20
41
䋭
4-fluoroamphetamine (4FMP)
4.45
14.81
154
109
20
31
䋭
4-fluoromethamphetamine
5.51
18.69
168
109
20
26
䋭
N,N-dimethylcathinone
3.86
7.71
178
105
20
41
䋭
ethcathinone
4.05
10.07
178
132
20
36
䋭
buphedrone
4.68
10.17
178
131
20
26
䋭
3-fluoromethcathinone
3.92
9.44
182
149
20
31
䋭
4-methylethcathinone
6.61
11.29
192
144
20
31
䋭
3-methylethcathinone
6.70
10.79
192
144
20
31
䋭
2-methylethcathinone
6.39
10.43
192
144
20
31
䋭
4-ethylmethcathinone
7.97
11.33
192
144
20
31
䋭
4-methylbuphedrone
7.32
10.08
192
144
20
31
䋭
3-methylbuphedrone
7.40
9.95
192
144
20
31
䋭
N-ethylbuphedrone
5.61
9.00
192
130
20
31
䋭
N-ethyl-N-methylcathinone
4.80
7.08
192
105
20
41
䋭
3,4-dimethylmethcathinone
8.05
12.86
192
159
20
26
䋭
2,3-dimethylmethcathinone
7.53
10.44
192
159
20
26
䋭
pentedrone
6.86
8.37
192
132
20
31
䋭
methedrone
4.98
13.06
194
161
20
36
䋭
-PPP
5.11
12.97
204
105
20
41
䋭
4-ethylethcathinone
9.18
11.35
206
144
20
31
䋭
4-methyl-N-methylbuphedrone
7.89
5.96
206
105
20
41
䋭
2C-E
10.45
17.94
210
178
20
26
䋭
4'-methyl-PPP
7.78
15.45
218
119
20
46
䋭
-PBP
6.46
11.27
218
91
20
51
䋭
butylone
5.89
10.43
222
174
20
31
䋭
bk-MDEA
5.27
10.64
222
174
20
31
䋭
-PVP
8.83
10.72
232
91
20
46
䋭
pentylone
8.10
9.08
236
188
20
31
䋭
eutylone
6.87
9.31
236
188
20
31
䋭
methoxetamine
7.89
8.36
248
121
20
21
䋭
5-IAI
7.48
13.89
260
116
20
36
䋭
3,'4'-MD-PBP
7.71
12.99
262
112
20
41
䋭
MDPV
10.03
11.84
276
135
20
51
䋭
2,3-MDPV
10.10
12.57
276
135
20
51
䋭
naphyrone
16.53
12.24
282
141
20
46
䋭
AH7921
14.32
16.16
329
173
20
56
䋭
25I-NBOMe
20.07
19.98
428
91
20
46
䋭
UR-144
26.95
18.06
312
125
20
56
䋭
RCS-4 2-methoxy isomer
23.41
7.09
322
135
20
51
䋭
RCS-4 3-methoxy isomer
25.10
10.37
322
135
20
51
䋭
RCS-4
24.94
10.23
322
135
20
51
䋭
JWH-015
23.81
8.32
328
155
20
56
䋭
JWH-073
25.22
11.48
328
155
20
56
䋭
XLR-11
25.61
12.41
330
125
20
66
䋭
JWH-203
25.68
12.98
340
125
20
61
䋭
JWH-022
25.48
12.04
340
155
20
51
䋭
JWH-016
25.09
11.41
342
155
20
76
䋭
JWH-018
26.33
15.23
342
155
20
76
䋭
JWH-122 N-(4-pentenyl) analog
26.45
14.71
354
169
20
66
䋭
JWH-122
27.30
19.60
356
169
20
66
䋭
JWH-007
26.25
14.14
356
155
20
61
䋭
JWH-019
27.42
20.39
356
155
20
61
䋭
AM-2201
25.03
10.50
360
155
20
51
䋭
APICA
25.89
14.05
365
135
20
86
䋭
APINACA (AKB48)
26.47
16.86
366
135
20
46
䋭
AB-FUBINACA
17.70
2.46
369
109
20
26
䋭
JWH-020
28.66
27.69
370
155
20
81
䋭
JWH-081
27.22
19.02
372
185
20
81
䋭
MAM-2201
25.99
13.22
374
169
20
71
䋭
STS-135
24.58
9.28
383
135
20
81
䋭
AKB48 N-(5-fluoropentyl) analog
25.09
10.87
384
135
20
41
䋭
EAM-2201
26.72
15.63
388
183
20
61
䋭
*Rt.㽲; HILIC elution, **Rt.㽳; Isocratic elution
㧙㧙
EP
9.0
7.0
9.0
7.0
6.5
12.0
7.5
8.5
8.5
8.5
8.5
8.5
8.5
8.5
7.5
10.5
10.5
12.0
9.0
10.0
8.0
9.0
12.0
8.5
7.5
8.5
8.5
7.0
12.0
12.0
11.0
11.5
12.0
8.0
8.0
11.5
8.0
10.5
10.5
12.0
12.0
12.0
8.0
8.0
10.5
8.5
12.0
4.5
4.5
6.5
10.0
9.0
9.0
11.0
11.5
9.0
6.5
10.0
8.0
9.0
10.5
12.0
12.0
CEP
8
10
8
8
8
12
10
10
10
10
10
10
10
16
10
8
8
8
8
10
10
16
10
8
10
10
10
10
10
10
12
10
14
14
14
14
14
18
14
14
14
14
12
12
24
12
12
16
16
14
14
16
16
14
14
14
14
16
16
14
14
14
16
CE
39
23
27
29
23
29
25
35
35
35
35
35
35
31
25
27
27
25
29
29
41
31
19
31
35
23
23
31
27
27
37
31
33
37
37
39
43
69
29
27
27
27
31
31
33
45
35
35
35
37
29
39
39
37
39
33
75
31
33
37
39
31
39
CXP
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
4
㧞 .%/5/5 ᴺߦࠃࠆ 2(2 ࠞ࡜ࡓࠍ↪޿ߚੂ↪⮎‛ߩಽᨆ᧦ઙߩᬌ⸛
.%/5/5 ᴺߢߪ‫ޔ‬ಽᨆኻ⽎߇੍߼ᮡḰṁᶧߦࠃࠅ࠴ࡖࡦࡀ࡞ࠍ૞ᚑߒߚ⮎‛ߦ㒢ቯߐࠇ
ࠆ߇‫৻╙ޔ‬Ბ㓏⋡ߩㄦㅦࠬࠢ࡝࡯࠾ࡦࠣࠍ⋡⊛ߣߒߡ 54/ ᴺߦࠃࠆ㜞ᗵᐲಽᨆᴺࠍᬌ⸛ߒ
ߚ‫ߡߒߣࡓ࡜ࠞޔߚ߹ޕ‬㜞ㅦ㜞ಽ㔌ࠍᔨ㗡ߦࠦࠕࠪࠚ࡞࠲ࠗࡊߩలႯ೷ࠞ࡜ࡓࠍ૶↪ߒߚ‫ޕ‬
⒖േ⋧ߩࠣ࡜ࠫࠚࡦ࠻ㅍᶧ᧦ઙߩᬌ⸛ߢߪ‫ޔ‬72.% ᴺߣห᭽ߦ৻ᢧಽᨆ᧦ઙࠍᬌ⸛ߒߚ߇‫ޔ‬
⮎‛㛽ᩰ᭴ㅧߦࠃߞߡࡇ࡯ࠢ଻ᜬᤨ㑆ߩ௑ะ߇ᄢ߈ߊ⇣ߥߞߚߎߣ߆ࠄ‫ޔ‬วᚑࠞࡦ࠽ࡆࡁ
ࠗ࠼♽ߪࠕࠗ࠰ࠢ࡜࠹ࠖ࠶ࠢㅍᶧߣߒ‫ࡦࡁ࠴ࠞޔ‬෸߮ࡈࠚࡀ࠴࡞ࠕࡒࡦ♽╬ߩ⮎‛ߪ
*+.+% ㅍᶧߣߒߚ‫⚿ߩߘޕ‬ᨐ‫ޔ‬ਔಽᨆ᧦ઙߦ߅ߌࠆฦ⮎‛ᚑಽߩઍ⴫⊛ߥࡇ࡯ࠢ଻ᜬᤨ㑆
෸߮ 54/ ࡄ࡜ࡔ࡯࠲࡯ߪ⴫ߦ␜ߒߚㅢࠅߣߥߞߚ‫ޔ߅ߥޕ‬ਔㅍᶧ᧦ઙߣ߽ࡇ࡯ࠢ଻ᜬᤨ㑆
߇⧯ᐓᄌേߒ߿ߔ޿ߎߣ߆ࠄ‫੹ޔ‬ᓟ‫ޔ‬⒖േ⋧᧦ઙࠍᡷༀߔࠆᔅⷐ߇޽ࠆߣ⠨߃ࠄࠇߚ‫߹ޕ‬
ߚ‫ޔ‬ᬌ಴ᗵᐲߪᬌ⸛ኻ⽎ߣߒߚో⮎‛ᮡḰṁᶧ PIO. Ớᐲߦߡᬌ಴น⢻ߢ޽ࠅ‫ޔ‬ᓥ᧪
ߩࠬࠠࡖࡦ᷹ቯߩᬌ಴ᗵᐲߣᲧセߒߡࠃࠅ㜞ᗵᐲߦಽᨆߔࠆߎߣ߇น⢻ߣߥߞߚ‫ޕ‬
㧟 ᮨᡆ⹜ᢱṁᶧߩᬌ⸛
Ꮢ⽼ࡂ࡯ࡉ࠹ࠖ ⒳㘃ߩࡔ࠲ࡁ࡯࡞᛽಴ᶧߢߪ‫ޔ‬72.%2&# ᴺߢߪ޿ߕࠇ߽ⶄᢙߩᅹኂࡇ
࡯ࠢ߇⹺߼ࠄࠇߡ߅ࠅ‫ ߩࠢ࡯ࡇ‛⮎ߪࠄࠇߎޔ‬2&# ࠬࡍࠢ࠻࡞ᬌ⚝ߦᡰ㓚ࠍ߈ߚߔ߽ߩߢ
޽ߞߚ‫ޔߒ߆ߒޕ‬54/ ᚻᴺߦࠃࠆ .%/5/5 ᴺߢߪ‫ޔ‬72.%2&# ᴺߦߡᬌ಴ߐࠇߚ⹜ᢱ↱᧪ߩ
ᅹኂࡇ࡯ࠢߪ⷗ࠄࠇߕ‫ޔ‬ᷝടߒߚ⮎‛ᚑಽࠍ․ቯߔࠆߎߣ߇น⢻ߢ޽ߞߚ‫ޕ‬࿑ߦ .%/5/5
ᴺߦࠃࠅಽᨆࠍⴕߞߚ ଀ࠍ␜ߔ‫ޕ‬
‫ޤ⺆⚿ޣ‬
㆑ᴺ࠼࡜࠶ࠣ⵾ຠߦ฽᦭ߐࠇࠆ⮎‛ߪᴺⷙ೙ߣߩ޿ࠊࠁࠆ̌ࠗ࠲࠴ࠧ࠶ࠦ̍߇⛯޿ߡ߅
ࠅ‫ޔ‬ᜰቯߐࠇࠆᜰቯ⮎‛ߩᢙߦߟ޿ߡ‫੹ޔ‬ᓟ߽Ⴧടߩ৻ㅜࠍߚߤࠆߎߣ߇ᗐቯߐࠇࠆ‫੹ޕ‬
ᓟߩ㆑ᴺ࠼࡜࠶ࠣߩಽᨆᴺࠍ⠨߃ࠆߦᒰߚࠅ‫ޔ‬ᄙߊߩ႐ว‫⇣ޔ‬ᕈ૕ߩ᭴ㅧߦࠃߞߡᴺⷙ೙
߇⇣ߥࠆ⃻⁁ࠍ⠨ᘦߒ‫ߩߘޔ‬ಽ㔌⏕⹺ࠍᒝ࿕ߦߔࠆߎߣ෸߮ᓸ㊂฽᦭⮎‛ߦ߽ኻᔕߔࠆ㜞
ᗵᐲಽᨆᚻᴺࠍ⏕┙ߒߡ߅ߊߎߣ߇‫ޔ‬ᜰቯ⮎‛╬ߩⷙ೙⮎‛ᬌᩏߩᦝߥࠆା㗬ᕈะ਄ߦߟ
ߥ߇ࠆߣ⠨߃ࠄࠇࠆ‫⎇ᧄޕ‬ⓥߦࠃߞߡᬌ⸛ߒߚಽᨆ᧦ઙࠍၮߦ‫੹ޔ‬ᓟ߽⮎‛ߩಽᨆ࠺࡯࠲
ࠍㅊട࡮᜛లߔࠆߎߣߦࠃߞߡ‫ࠅࠃޔ‬ㄦㅦ߆ߟା㗬ᕈ߇㜞޿㆑ᴺ࠼࡜࠶ࠣߩಽᨆࠍⴕ޿‫ޔ‬
ᦝߥࠆ⮎‛ੂ↪ߩ㒐ᱛߦነਈߢ߈ࠆ߽ߩߣ⠨߃ߡ޿ࠆ‫ޕ‬
‫ޣ‬ෳ⠨ᢥ₂‫ޤ‬
⼊ኤᐡೃ੐ዪ⚵❱‽⟋ኻ╷ㇱ⮎‛㌂ེኻ╷⺖㧘ᐔᚑ ᐕᐲ਄ඨᦼߩ⮎‛࡮㌂ེᖱ൓
㧔ᥳቯ୯㧕㧘ᐔᚑ ᐕ ᦬
ో࿖ߩ♖␹⑼ක≮ᣉ⸳ߦ߅ߌࠆ⮎‛㑐ㅪ♖␹∔ᖚߩታᘒ⺞ᩏ㧘ᐔᚑ ᐕᐲෘ↢ഭ௛⑼
ቇ⎇ⓥ⾌⵬ഥ㊄
ක⮎ຠ࡮ක≮ᯏེ╬࡟ࠡࡘ࡜࠻࡝࡯ࠨࠗࠛࡦࠬ✚ว⎇ⓥ੐ᬺಽᜂ⎇
ⓥႎ๔ᦠ㧔⎇ⓥಽᜂ⠪ ᧻ᧄବᒾ㧕
⧎ዥ
ᧁୖℲℂࠄ㧘㆑ᴺ࠼࡜࠶ࠣࠍขࠅᏎߊ࿖ౝᄖߦ߅ߌࠆ⃻⁁ߣⷙ೙ߦߟ޿ߡ㧘⮎
ቇ㔀⹹㧘㧘
㧙㧙
࿑ ⮎‛ᷝടࡂ࡯ࡉ࠹ࠖ᛽಴ᶧߩ SRM ࠢࡠࡑ࠻ࠣ࡜ࡓ
(A) HILIC elution‫(ޔ‬B) Isocratic elution
‫૶⾌⚻ޣ‬ㅜ᣿⚦‫ޤ‬
૶ㅜ᣿⚦
㊄㗵
౞
ੂ↪ൻቇ‛⾰ᮡḰຠ⾼౉⾌
วᚑࠞࡦ࠽ࡆࡁࠗ࠼‫⾰‛ ╬‛⮎♽ࡦࡁ࠴ࠞޔ‬㧕
ಽᨆ↪ࠞ࡜ࡓ╬⾼౉⾌㧔#ESWKV[72.%*552(2 ࠞ࡜ࡓ‫╬ࡓ࡜ࠞ࠼࡯ࠟޔ‬㧕
᦭ᯏṁᇦ╬⹜⮎⾼౉⾌㧔ࠕ࠮࠻࠾࠻࡝࡞‫ࡓ࠙࠾ࡕࡦࠕ㉄߉ޔ‬㧕
ࠟ࡜ౕེࠬ㘃⾼౉⾌㧔ࠟ࡜ࠬࡃࠗࠕ࡞↉‫࠻࡯ࠨࡦࠗࠬ࡜ࠟޔ‬㧕
੐ോ⾌㧔ᝄㄟߺᚻᢙᢱ ࿁ಽ㧕
ว⸘㧔㗍㊄೑ᕷ ౞฽߻㧕
㧙㧙

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