Kobe University Repository : Kernel

　Kobe
University Repository : Kernel
Title
Experimental Staphylococcus hyicus Infection in Piglets
Author(s)
Shimizu, Akira / Kawano, Junichi
Citation
神戸大学農学部研究報告, 18(2): 207-212
Issue date
1989-01
Resource Type
Departmental Bulletin Paper / 紀要論文
Resource Version
publisher
URL
http://www.lib.kobe-u.ac.jp/handle_kernel/00200508
Create Date: 2015-01-31
神大農研報
C
S
c
i
.H
e
pt
.F
a
c
.A
g
r
.K
o
b
cU
n
i
v
.
)1
8・2
0
7
2
1
2
.1
開9
EXPERIMENTAL STAPHYLOCOCCUSHYICUS
INFECTION IN PIGLETS
Akira SHIMIZU~
Kosuke OKADAヤ
Shige KIMURA~
Junichi KAWANO~
Haruka TERANISHI~
and Kazumi SUGIHAHA川本
C
H
e
c
e
i
v
e
df
o
rp
u
bi
1c
a
t
i
o
no
nA
u
g
u
s
t1
0，1
9
8
8
)
A
b
s
t
r
a
c
t
fStα
:
ph
y
l
o
c
o
c
c
u
sh
y
i
c
u
si
s
o
l
a
t
e
d
Twos
t
r
a
i
n
s Cphage t
y
p
e
s89/839/8188 and 89/8188) o
fromp
i
g
sw
i
t
he
x
u
d
a
t
i
v
ee
p
i
d
e
r
m
i
t
i
s (EE) were u
s
e
di
nt
h
i
se
x
p
e
r
i
m
e
n
ti
no
r
d
e
rt
os
t
u
d
y
t
h
ep
a
t
h
o
g
e
n
e
s
i
so
fEE i
np
i
g
s
. Thed
i
s
e
a
s
e was r
e
p
r
o
d
u
c
e
di
nt
h
ep
i
g
l
e
t
so
f3
0 days o
l
d
tu
r
e
so
ft
h
e 2s
t
r
a
i
n
s were i
n
o
c
u
l
a
t
e
ds
u
b
c
u
t
a
n
e
o
u
s
l
yo
r by s
c
a
r
i
f
i
c
a
t
i
o
n
. A
l
l
whent
h
ec
u1
f
o
ft
h
e 3p
i
g
sshowed mild e
x
u
d
a
t
i
v
eg
r
o
s
sl
e
s
i
o
n
so
f EE. H
i
s
t
o
l
o
g
i
c
a
l
l
y，accumulation o
c
e
l
l
u
l
a
rd
e
b
r
i
si
n
c
l
u
d
i
n
gc
o
c
c
o
i
d organisms was o
b
s
e
r
v
e
d on t
h
ee
p
i
d
e
r
m
a
ls
u
r
f
a
c
e
. The
.h
y
i
c
u
si
s
f
a
c
tt
h
a
t an i
n
f
e
c
t
i
o
n was e
x
p
e
r
i
m
e
n
t
a
l
l
yi
n
d
u
c
e
d by i
n
o
c
u
l
a
t
i
o
np
r
o
v
e
st
h
a
tS
.
r
e
s
p
o
n
s
i
b
l
ef
o
rp
a
t
h
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n
e
s
i
so
fswineEE
I
ntroduction
Exudative e
p
i
d
e
r
m
i
t
i
s (EE) i
sas
k
i
nd
i
s
e
a
s
et
h
a
ta
f
f
e
c
t
smainly s
u
c
k
l
i
n
gp
i
g
so
f one
s commonly
month and downward， and i
r
e
fe
r
r
e
dt
oa
sg
r
e
a
s
yp
i
gd
i
s
e
a
s
e
. The d
i
s
e
a
s
e
'I
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"
O
L
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n
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ss
a
i
dt
ob
ei
n
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r
o
d
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c
e
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i
r
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t by 8I
nG
e
r
1842 a
st
h
e name o
f “Hautauschlag" i
fter t
h
a
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t
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c
c
u
r
r
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n
c
ehas b
e
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many6，7). A
r
e
p
o
r
t
e
d i
n e
a
c
h o
f European c
o
u
n
t
r
i
e
s，
A
u
s
t
r
a
l
i
aand t
h
eUnited 8
t
a
t
e
s
. I
nJapan1
!
)，
e
v
e
rs
i
n
c
et
h
eo
c
c
u
r
r
e
n
c
ei
nN
i
i
g
a
t
aP
r
e
f
e
c
t
u
r
e
ob
et
h
ef
i
r
s
t
. numeri
n1
9
6
6，whichseemed t
6
)haved
e
a
l
twitht
h
i
sd
i
s
o
u
si
n
v
e
s
t
i
g
a
t
o
r
s
l
5，1
1
2
s
e
a
s
ei
nt
h
e
i
rr
e
p
o
r
t
s
. We )a
l
s
oe
n
c
o
u
n
t
e
r
e
d
an outbreak o
f EE i
na l
i
t
t
e
ro
f 7 newborn
p
i
g
so
f 2 -3 days o
l
di
n a farm i
n Hyogo
s
o
l
a
t
e
dStα
p
h
y
l
o
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o
c
c
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sh
y
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c
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s
P
r
e
f
e
c
t
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r
e，andi
i
np
u
r
ec
u
l
t
u
r
e from t
h
es
k
i
nl
e
s
i
o
n
so
ft
h
e
h
e
a
f
f
e
c
t
e
d p
i
g
s， and furthermore， from t
i
v
e
r，
parenchymatous organs s
u
c
ha
sh
e
a
r
t，l
k
i
d
n
e
y
sand s
p
l
e
e
no
ft
h
ep
i
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i
e
d
.
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，6
，札 1
4
)i
n Europe have
80mei
n
v
e
s
t
i
g
a
t
o
r
sl
-
*Laboratoryo
fA
n
i
m
a
lf
l
y
g
i
c
n
c
*
*F
a
c
u
l
t
yo
fA
ε
r
i
c
u
l
t
u
r
c，l
w
a
t
aマ
しn
i
v
e
r
s
i
t
y
*
*
*Laboratory(
)
fA
n
i
m
a
lM
a
n
a
g
e
m
c
nt
.
attemptede
x
p
e
r
i
m
e
n
t
a
li
n
f
e
c
t
i
o
no
fS
.h
y
i
c
u
s
i
np
i
g
l
e
t
s
. I
n 1953，80MPOLINSKyI3) i
s
o
l
a
t
e
d
ag
r
a
m
p
o
s
i
t
i
v
ec
o
c
c
u
s from t
h
ep
i
g
s with
e s
u
c
c
e
e
d
e
d i
n r
e
p
r
o
d
u
c
i
n
g t
h
e
EE， and h
d
i
s
e
a
s
e by i
n
o
c
u
l
a
t
i
n
gc
u
l
t
u
r
es
u
s
p
e
n
s
i
o
no
f
t
h
i
s organism i
n
t
o t
h
e a
p
p
a
r
e
n
t
l
y h
e
a
l
t
h
y
p
i
g
s
. He named t
h
i
sg
r
a
m
p
o
s
i
t
i
v
ec
o
c
c
u
s
Micrococcus h
y
i
c
u
s
. Later i
n 1978，t
h
eo
r
:
p
h
y
ganism was c
l
a
s
s
i
f
i
e
da
st
h
e genus Stα
l
o
c
o
c
c
u
s， and was d
e
s
i
g
n
a
t
e
d Stα
:
p
h
y
l
o
c
o
c
c
u
s
tαl
.
4
)
h
y
i
c
u
sby DEVHIESEe
T
h
i
s paper d
e
s
c
r
i
b
e
s an e
x
p
e
r
i
m
e
n
t
a
l
l
y
i
n
d
u
c
e
di
n
f
e
c
t
i
o
ni
np
i
g
l
e
t
s by u
s
i
n
gs
t
r
a
i
n
s
y
i
c
u
si
s
o
l
a
t
e
d from p
i
g
s with EE.
o
fS
.h
Materials and Methods
S
.h
y
i
c
u
ss
t
r
a
i
n
sand c
u
l
t
i
v
a
t
i
o
n
y
i
c
u
s (phage t
y
p
e
so
f
Two s
t
r
a
i
n
so
fS
.h
89/839/8188and 89/8188) i
s
o
l
a
t
e
d from p
i
g
s
I
2
with EE )were u
s
e
di
nt
h
i
se
x
p
e
r
i
m
e
n
t
. The
s
t
r
a
i
n
shad b
e
e
np
r
e
s
e
r
v
e
di
nt
r
y
p
t
o・soyagar
)s
t
a
bc
u
l
t
u
r
e
sa
tambient temperature
(
N
i
s
s
ui
u
n
t
i
lt
h
e
y were u
s
e
d
. I
no
r
d
e
rt
op
r
e
p
a
r
e
h
es
t
o
c
kc
u
l
t
u
r
e
so
fS
.h
y
i
c
u
s
t
h
e inoculum，t
were t
r
a
n
s
p
l
a
n
t
e
dt
o 5% s
h
e
e
p blood agar
o
r1
8hours，and t
h
e
n
and i
n
c
u
b
a
t
e
da
t3
7C f
t
h
e
y were c
u
l
t
i
v
a
t
e
di
nb
r
a
i
nh
e
a
r
ti
n
f
u
s
i
o
n
0
2
0
8
A
.SHIMIZU，K.OKADA，，
1
.KAWANO
，H
.TERANISHI，S
.KIMURAand K
.SUGIHARA
Table 1
. Experimentald
e
s
i
g
n
Inoculum
I
n
o
c
u
l
a
t
e
dp
ig
-s
Age
8
t
r
a
i
nNo.
Route本
Dose*
*
8ex
Weight
3
0
f
e
m
a
l
e
5
.
0kg
NO.9
(
8
9
/
8
3
9
/
8
1
8
8
)
8C
8CN
1ml
1ml
2
3
0
male
4.
4kg
No. 3
8
(
8
9
/
8
1
8
8
)
8C
8CN
1ml
1ml
3
3
0
f
e
m
a
l
e
5
.
0kg
8
NO.9+No.3
8C
8CN
1ml
1ml
No.
(Days)
(Phage t
y
p
e
)
*8C=8ubcutaneous， 8CN=Scarification.
川
Inoculum:1
8
h
o
u
rBHIb
r
o
t
hc
u
l
t
u
r
e
.
o
c
) broth (Nissui
) with shaking a
t 37
(BHI
f
o
r1
8h
o
u
r
s
.
Experimental p
i
g
s
Three Landrace p
i
g
l
e
t
so
f 30 days o
l
d wei
g
h
i
n
gabout5 kg were s
e
l
e
c
t
e
d from a l
i
t
t
e
r
and u
s
e
df
o
rt
h
i
se
x
p
e
r
i
m
e
n
t
. P
r
i
o
rt
ot
h
e
experiment， n
a
s
a
l and s
k
i
n swab samples
were obtained from e
a
c
hp
i
g
l
e
tt
ob
a
c
t
e
r
i
o
l
o
g
i
c
a
l
l
ya
s
c
e
r
t
a
i
nt
h
a
tt
h
ep
i
g
s were not i
n
f
e
c
t
e
d withS
.h
y
i
c
u
s
.
Experimental i
n
f
e
c
t
i
o
n
Experimental p
r
o
c
e
d
u
r
ei
s summarized i
n
Table 1
. A volume o
f 1 ml o
ft
h
e 18-hour
BHI broth c
u
l
t
u
r
e was subcutaneously i
n
o
c
u
h
i
l
e
l
a
t
e
di
n
t
ot
h
er
i
g
h
te
a
ro
fe
a
c
hp
i
g
l
e
t，w
t
h
esame amount was i
n
o
c
u
l
a
t
e
dbys
c
a
r
i
f
i
c
a
t
i
o
ni
n
t
ot
h
el
e
f
te
a
ro
f each p
i
g
l
e
t
. Pig
No.1 was g
i
v
e
nas
t
r
a
i
no
f phage t
y
p
e 89/
839/8188o
n
l
y，and p
i
g No.2 was g
i
v
e
n 89/
8188 o
n
l
y
. Pig No.3 was g
i
v
e
n a mixture
o
fe
q
u
a
lmeasureso
f phage t
y
p
e
s89/839/8188
and 89/8188. A
l
lt
h
ep
i
g
l
e
t
s were examined
f
o
rc
l
i
n
i
c
a
ls
i
g
n
sd
a
i
l
yf
o
r 7p
o
s
t
i
n
o
c
u
l
a
t
i
o
n
d
a
y
s
.
Recovery o
f orgamisms
Recoveryo
forganismswasconductedfrom
t
h
en
a
s
a
lc
a
v
i
t
y and s
k
i
ns
u
r
f
a
c
eo
f body o
f
e
a
c
hp
i
g
l
e
tu
s
i
n
gcotton swab on p
o
s
t
i
n
o
c
u
l
a
t
i
o
n day 2
. The c
o
t
t
o
n sawb samples taken
frome
a
c
hp
i
g
l
e
t was d
i
r
e
c
t
l
yt
r
a
n
s
p
l
a
n
t
e
dt
o
)andc
.h
y
i
c
u
ss
e
l
e
c
t
i
v
e agar mediuma
u
l
t
h
eS
7C f
o
r 48 h
o
u
r
s
. I
d
e
n
t
i
f
i
c
a
t
i
o
no
f
t
u
r
e
da
t3
t
h
er
e
c
o
v
e
r
e
d organisms was done c
o
n
forming
t
ot
h
ec
r
i
t
e
r
i
ad
e
s
c
r
i
b
e
dbyDBVHIESBe
tα1
.1)
A
l
lt
h
ep
i
g
l
e
t
s were exsanguinated f
o
r auto
psy on t
h
ep
o
s
t
i
n
o
c
u
l
a
t
i
o
n day 7
. Recovery
o
ft
h
e inoculum organisms was attempted
e
v
e
n from t
h
ei
n
t
e
r
n
a
l organs (
h
e
a
r
t，l
i
v
e
r，
l
u
n
g
s， s
p
l
e
e
n， and kidneys) and s
u
p
e
r
f
i
c
i
a
l
lymph nodes o
fe
a
c
hp
i
g
l
e
t
.
Hi
s
t
o
p
a
t
h
o
l
o
g
i
c
a
l examination
H
i
s
t
o
p
a
t
h
o
l
o
g
i
c
a
l examination o
ft
h
es
k
i
n
l
e
s
i
o
n
so
fe
a
c
hp
i
g
l
e
t was conducted on t
h
e
p
o
s
t
i
n
o
c
u
l
at
i
o
n day 7
. P
i
e
c
e
so
fs
k
i
nt
i
s
s
u
e
weref
i
x
e
di
n 10% n
e
u
t
r
a
l formalin s
o
l
u
t
i
o
n，
and t
h
e
n embedded i
np
a
r
a
f
f
i
n
.8
e
c
t
i
o
n
s were
s
t
a
i
n
e
d with hematoxylin and e
o
s
i
n (HE).
0
白
Results
C
Ii
n
i
c
a
lf
i
n
d
i
n
g
s
y
i
c
u
s
A
l
lt
h
ep
i
g
l
e
t
si
n
o
c
u
l
a
t
e
d with S
.h
s
t
r
a
i
n
s hadmildc
l
i
n
i
c
a
ls
i
g
n
so
fEE
. Exudat
i
o
n，hyperemia，and s
w
e
l
l
i
n
g were observed
i
nt
h
ei
n
o
c
u
l
a
t
e
ds
i
t
e
so
ft
h
es
k
i
ni
ne
a
c
h
p
i
g
l
e
t 1-2daysa
f
t
e
ri
n
o
c
u
l
a
t
i
o
n
. Thes
u
p
e
r
f
i
c
i
a
ll
a
y
e
ro
ft
h
es
k
i
n was s
of
r
a
i
la
st
ob
e
p
e
e
l
e
d even by rubbing with c
o
t
t
o
n swab.
h
e
Three t
o f
o
u
r days a
f
t
e
r i
n
o
c
u
l
a
t
i
o
n， t
幽
2
0
9
ExperimentalS
.h
y
i
c
u
sI
n
f
e
c
t
i
o
ni
nP
i
g
l
e
t
s
y
i
c
u
sfroms
k
i
n
so
fi
n
f
e
c
t
e
dp
i
g
s
Table2
. Recoveryo
fS
.h
o
l
o
n
i
e
sr
e
c
o
v
e
r
e
d*
No
.o
fS
. hyicusc
Pig
No.
E
y
e
l
i
d
Naris
Ear
Abdomen
L
e
f
t
2
+++
+++
3
+十+
Thigh
+
+
Dorsum
十
+
*No. ofS
.h
y
i
c
u
sc
o
l
o
n
i
e
s
/
o
n
ep
l
a
t
e，一:nor
e
c
o
v
e
r
y，十:l
e
s
sthan5
0c
o
l
o
n
i
e
s，
++:
5
0- 1
0
0c
o
l
o
n
i
e
s，++十:moret
h
a
n1
0
0c
o
l
o
n
i
e
s
.
Table3
. Recoveryo
fS
.h
y
i
c
u
sfromi
n
t
e
r
n
a
lorgansand lymphnodeso
fi
n
f
e
c
t
e
dp
i
g
s
No. o
fS
. hyicusc
o
l
o
n
i
e
sr
e
c
o
v
e
r
e
d*
Pig
No.
Lymphnode
Organ
Heart
Lung
L
i
v
e
r
Submaxi- P
t
i
d
S
p
l
e
e
n Kidney ~~ií~;;".
2
3
*For
十
C
e
r
v
i
- R
e
t
r
o
- Poplト
c
a
l pharynx t
e
a
l
+++
++
十+十
十++
+
+
+
+
t
o +十+s
e
eremarkso
fTable2
.
l
e
s
i
o
n
s were found t
a
k
i
n
ga t
u
r
nf
o
rr
e
s
t
o
r
a
h
i
n firm
t
i
o
n and d
r
y
i
n
gu
p
. Furthermore，t
s
c
a
b
s were observed c
o
v
e
r
i
n
gt
h
ei
n
o
c
u
l
a
t
e
d
s
i
t
e
s5-6 days a
f
t
e
ri
n
o
c
u
l
a
t
i
o
n
.
Autopsy
N0 g
r
o
s
sl
e
s
i
o
n
s were r
e
c
o
g
n
i
z
e
di
n t
h
e
i
n
t
e
r
n
a
l organs i
ne
i
t
h
e
ro
ft
h
ei
n
o
c
u
l
a
t
e
dp
i
g
l
e
t
s
.
Recovery o
f organisms
I
n each o
ft
h
e 3p
i
g
l
e
t
s， t
h
e inoculum
organisms were r
e
c
o
v
e
r
e
di
nl
a
r
g
e numbers
from t
h
es
k
i
no
ft
h
el
e
f
te
a
r，but not from
t
h
es
k
i
no
ft
h
er
i
g
h
te
a
r (Table 2
)
. The i
n
oculum organisms were a
l
s
or
e
c
o
v
e
r
e
d from
t
h
e hypogastric s
k
i
no
fp
i
g
s No.1and No.2
i
n small numbers. Table 3 i
n
d
i
c
a
t
e
st
h
er
e
s
u
l
t
so
fr
e
c
o
v
e
r
yo
ft
h
e inoculum organisms
from t
h
e i
n
t
e
r
n
a
l organs and s
u
p
e
r
f
i
c
i
a
l
lymph n
o
d
e
s
. The inoculum organisms were
not r
e
c
o
v
e
r
e
d from t
h
ei
n
t
e
r
n
a
l organs s
u
c
h
倫
刷
a
sh
e
a
r
t，l
u
n
g
s，s
p
l
e
e
n，k
i
d
n
e
y
s，and l
i
v
e
ri
n
h
i
l
ec
o
n
e
i
t
h
e
ro
ft
h
ei
n
o
c
u
l
a
t
e
dp
i
g
l
e
t
s， w
s
i
d
e
r
a
b
l
e numbers o
f them were i
s
o
l
a
t
e
d
from t
h
e p
a
r
o
t
i
d lymph nodes i
n a
l
l t
h
e
p
i
g
l
e
t
s
.
Hi
s
t
o
p
a
t
h
o
l
o
g
y
Locationsands
e
v
e
r
i
t
y of h
i
s
t
o
p
a
t
h
o
l
o
g
i
c
a
l
1t
h
ep
i
g
sa
r
e summarized
s
k
i
nl
e
s
i
o
n
si
n al
i
n Table4
.
8
i
t
eo
fi
n
o
c
u
l
a
t
i
o
n
: There was no remarka
b
l
ed
i
f
f
e
r
e
n
c
ei
ns
e
v
e
r
i
t
y of t
h
es
k
i
nl
e
s
i
o
n
s
o
ft
h
ee
a
r between t
h
ep
i
g
si
n
o
c
u
l
a
t
e
d with
phaget
y
p
e
s 89/S39/8188and t
h
o
s
ei
n
o
c
u
l
a
t
e
d
with 8
9
/
8
1
8
8
. 8
i
m
i
l
a
rh
i
s
t
o
p
a
t
h
o
l
o
g
i
c
a
lf
i
n
d
i
n
g
s were obtained from t
h
el
e
s
i
o
n
si
nt
h
e
r
i
g
h
t and l
e
f
te
a
rs
k
i
n， and t
h
ee
a
rl
e
s
i
o
n
s
were c
h
a
r
a
c
t
e
r
i
z
e
d by t
h
e following f
i
n
d
i
n
g
s
.
1u
l
a
r d
e
b
r
i
s i
n
c
l
u
d
i
n
g
Accumulation o
f c
el
c
o
c
c
o
i
d organisms was observed on t
h
e
，2
)
.
p
a
r
a
k
e
r
a
t
o
t
i
c epidermal s
u
r
f
a
c
e
'(
F
i
g
s
.1
2
1
0
A
.SHIMIZU，K.OKADA，J
.KAWANO
，H
.T
E
R
A
N
I
S
I
I
J，S
.KJMUHAand K
.S
L
'G
I
I
I
AHA
Table4
. H
i
s
t
o
l
o
g
i
c
a
ls
e
v
e
r
i
t
yo
fe
x
u
d
a
t
i
v
ee
p
i
d
e
r
m
i
t
i
si
np
i
g
si
n
o
c
u
l
a
t
e
d
w
i
t
hS
.h
y
i
c
u
s*
Pig
8
t
r
a
i
nNo.
No.
(Phaget
y
p
e
)
No.9
(89/839/8188)
2
3
NO.38
(
8
9
/
8
1
8
8
)
NO.9+No.3
8
Ear
Abdomen
E
y
e
l
i
d
L
e
f
t本*
Right**ホ
十十
+十十***ホ
十十+
十++****
ND
+++
+十十****
+
Dorsum
Fore
p
a
r
t
Middle
p
a
r
t
Rear
p
a
r
t
+
十
十十
* 十十 +:s
e
v
e
r
echanges，十十:moderate，十 :mild， - :noremarkable，ND:n
o
td
o
n
e
.
*
*Inoculatedbys
c
a
r
i
f
i
c
a
t
i
o
n
.
*
*
*8ubcutaneousinoculation
*
*
*
*Accompaniedwithabscessformationa
ts
i
t
eo
fi
n
o
c
u
l
a
t
i
o
n
.
There was a
c
a
n
t
h
o
s
i
so
ft
h
ee
p
i
d
e
r
m
i
s
. The
dermis was i
n
f
i
l
t
r
a
t
e
d by h
i
s
t
i
o
c
y
t
e， lympho
c
y
t
e，ande
o
s
i
n
o
p
h
i
lwitha
b
s
c
e
s
s
e
sformation
a
tt
h
es
i
t
eo
fi
n
o
c
u
l
a
t
i
o
n(
F
i
g
.
3
)
.
Others
k
i
n
:P
a
s
t
u
l
a
rl
e
s
i
o
n
s were o
b
s
e
r
v
e
d
ont
h
eepidermal s
u
r
fa
c
eo
ft
h
es
k
i
no
f lower
abdomen C
F
i
g
.
4
)
.
Discussion
EE i
se
x
p
e
r
i
m
e
n
t
a
l
l
yr
e
p
r
o
d
u
c
i
b
l
ebyi
n
o
c
u
l
a
t
i
n
gp
i
g
l
e
t
swithS
.h
y
i
c
u
s1.a
，6，
8，14). I
nt
h
i
s
.h
y
i
c
u
si
s
o
l
a
t
e
d from
study，two s
t
r
a
i
n
so
fS
p
i
g
s with EE were u
s
e
da
si
n
o
c
u
l
ai
no
r
d
e
r
t
or
e
p
r
o
d
u
c
et
h
ed
i
s
e
a
s
e
. The p
i
g
si
n
o
c
u
l
a
t
e
d
withe
a
c
ho
ft
h
es
t
r
a
i
n
sd
e
v
e
l
o
p
e
d both g
r
o
s
s
and h
i
s
t
o
l
o
g
i
c
a
l l
e
s
i
o
n
s i
n t
h
e i
n
o
c
u
l
a
t
e
d
s
it
e
s
. These g
r
o
s
s and h
i
s
t
o
l
o
g
i
c
a
ll
e
s
i
o
n
s
o
b
s
e
r
v
e
di
nt
h
i
s study were s
i
m
i
l
a
rt
ot
h
o
s
e
6
9
r
e
p
o
r
t
e
dbyJONES
)，L
)
'ECCYERandJERICH(
1
0
and MEBUSe
ta
l
.)
Concerning t
h
ep
a
t
h
o
g
e
n
i
c
i
t
yo
f swine S
.
h
y
i
c
u
ss
t
r
a
i
n
s f
o
r p
i
g
s， HU~TER e
t α.
l5)
s
t
a
t
e
di
nt
h
e
i
rr
e
p
o
r
tt
h
a
tt
h
es
t
r
a
i
n
si
s
o
l
a
t
e
d
from p
i
g
s with EE showed p
a
t
h
o
g
e
n
i
c
i
t
y
when i
t was i
n
o
c
u
l
a
t
e
di
n
t
op
i
g
l
e
t
s，but t
h
e
s
t
r
a
i
n
si
s
o
l
a
t
e
d from a
p
p
a
r
e
n
t
l
yh
e
a
l
t
h
yp
i
g
s
3
d
i
dn
o
t
. On t
h
e o
t
h
e
r hand， DIWH
.
IE
S
E)
s
t
a
t
e
dt
h
a
tt
h
e
r
e was no remarkable d
i
f
f
e
r
e
n
c
ei
np
a
t
h
o
g
e
n
i
c
i
t
yf
o
rp
i
g
s between t
h
e
inoculum s
t
r
a
i
n
so
fS
.h
y
i
c
u
si
s
o
l
a
t
e
d from
p
i
g
s with EE and t
h
o
s
ei
s
o
l
a
t
e
d from p
i
g
s
without EE.
I
ti
sw
e
l
l known t
h
a
t EE f
r
e
q
u
e
n
t
l
ya
t
t
a
c
k
ss
u
c
k
l
i
n
gp
i
g
sbetween 1and 20 days o
f
o e
x
p
e
r
i
a
g
e
. T
h
e
r
e
f
o
r
e， we attempted t
mentallyi
n
d
u
c
eani
n
f
e
c
t
i
o
nu
s
i
n
gtwop
i
g
l
e
t
s
o
f2days o
f age i
n our p
r
e
v
i
o
u
s study CData
a
r
e n
o
t shown). Although t
h
e inoculum
s
t
r
a
i
n
su
s
e
d were d
i
f
f
e
r
e
n
t from t
h
o
s
ei
nt
h
e
p
r
e
s
e
n
t study，none o
ft
h
ep
i
g
l
e
t
sg
i
v
e
nt
h
e
s
t
r
a
i
n
sshowed c
l
i
n
i
c
a
ls
i
g
n
so
fEE.
t was estimated t
h
a
t
From t
h
e
s
er
e
s
u
l
t
s，i
t
h
e
r
ea
r
es
e
v
e
r
a
lf
a
c
t
o
r
si
n
v
o
l
v
e
di
ni
n
f
e
c
t
i
o
n
o
f EE s
u
c
ha
sv
i
r
u
l
e
n
c
eo
fs
t
r
a
i
n
s， and
s
u
s
c
e
p
t
i
b
i
l
i
t
yo
fp
i
g
s
. At a
l
le
v
e
n
t
s， i
ti
s
c
e
r
t
a
i
n thatS
.h
y
i
c
u
s has pat
h
o
g
e
n
i
ce
f
fe
c
t
onp
l
g
S
.
References
1
) A¥
1T
SBEHG，G
.
:D
t
s
c
h
.T
i
e
r
αe
r
z
t.
l Wo
c
h
e
n
s
c
h
l
・
.
， 8
5， 433-438， 1
9
7
8
.
1T
SBEHG，G
. andS
. HAlEM: B
e
T
・
.
lT
i
e
r
2
) A¥
・
.
， 9
1， 2
9
9
3
0
1， 1
9
7
8
.
a
e
r
z
t
l
. WochenschT
、T
H
I
E
S
E， L
.A
.
: Am. J
.V
e
t
.R
e
s
.， 38，
3
) D氏
7
8
7
7
9
2，1
9
7
7
.
4
) DI
刊 H
I
E
S
E， L
.A
.， V
. HA，
J
E
K， P
. OE J) I~G ，
8.A.M日YEHandK.H
.S
C
H
L
E
I
F
E
l
t
:I
n
t
..
1
.
S
y
s
t
. Bαc
t
e
r
i
ol
.
， 2
8， 482-490， 1
9
7
8
.
E
x
p
e
r
i
m
e
n
t
a
lS
.h
y
i
c
u
sI
n
f
e
c
t
i
o
ni
nP
i
g
l
e
t
s
5
) HUNTEH，D
.，J
.N.TODD and 恥1
.LAHKIN:
B
r
.V
e
t
.J
.，126，225-229，1970.
6
) JONES，L
.D
.
: Am. J
.V
e
t
.R
e
s
.，17，179
193，1
9
5
6
.
.D
.
:V
e
t
. Med.，56，95-1ω ，1
0
0
1
.
7
) JOKES，L
8
)L
'ECUYEH，C
.
:C
αn
.J
. Comp. Med. V
e
t
.
S
c
i
.，30，9-16，1
9
6
6
.
9
)L
'ECUYEH，C
. and K.JEHICHO: C
αn
.J
.
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Comp. Med. V
e
t
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c
i
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1
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J
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h
o
l
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e
t
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9
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in Japanese).
1
2
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:
v
1I
Z
C， A.， K.OKAD九 J
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冊
，
2
1
1
TEHANISHI，S
.KIMUHA，T
.YABIKI and 1
.
NANJYO: S
c
i
.R
e
p
t
.，
Fαc
.A
g
r
.KobeU
n
i
v
.，
17，259267，1987 C
in Japanese with Eng
l
i
s
h summary).
.
:S
c
h
w
e
i
z
.A
r
c
h
. T
i
e
r
1
3
) SOMPOLINSKY， D
9
5
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e
i
l
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d
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1
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) UNDEHDAHL，N.R
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e
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) WATANABE， T
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s
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凶
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Explanation o
ff
i
g
u
r
e
s
F
i
g
.1
. Inoculateds
i
t
e 7days a
f
t
e
rs
c
a
r
i
f
i
c
a
t
i
o
nC
le
f
t ear s
k
i
no
f No.1
)
. Accumulation o
f
p
a
r
a
k
e
r
a
t
o
t
i
cc
e
l
l
u
l
a
rd
e
b
r
i
si
n
c
l
u
d
i
n
gc
o
c
c
o
i
d organisms i
ss
e
e
n on t
h
e epidermal
s
u
r
f
a
c
ewitha
c
a
n
t
h
o
s
i
so
ft
h
ee
p
i
d
e
r
m
i
s
. I
n
f
i
l
t
r
a
t
i
o
no
fh
i
s
t
o
c
y
t
i
cc
e
l
l，lymphocyte
0
.
ande
o
s
i
n
o
p
h
i
li
ss
e
e
ni
nt
h
ed
e
r
m
i
s
. HEs
t
a
i
n，X 6
F
i
g
.2
. I
n
o
c
u
l
a
t
e
ds
i
t
e 7 days a
f
t
e
r subcutaneous i
n
o
c
u
l
a
t
i
o
n C
r
i
g
h
t ear s
k
i
no
f No. 2
)
.
Accumulation o
f degenerated n
e
u
t
r
o
p
h
i
l
s and c
e
l
l
u
l
a
rd
e
b
r
i
s i
n
c
l
u
d
i
n
g c
o
c
c
o
i
d
organismsont
h
eepidermals
u
r
f
a
c
e，a
c
a
n
t
h
o
s
i
so
ft
h
eepidermis，andi
n
f
i1
tr
a
t
i
o
no
f
0
.
inframatoryc
e
l
li
nt
h
edermisa
r
eo
b
s
e
r
v
e
d
. HEs
t
a
i
n，X 6
F
i
g
.3
. I
n
o
c
u
l
a
t
e
ds
i
t
e 7 days a
f
t
e
r subcutaneous i
n
o
c
u
l
a
t
i
o
nC
r
i
g
h
t ear s
k
i
no
f No. 3
)
.
Abscess i
s formed i
ni
n
o
c
u
l
a
t
e
ds
i
t
eo
ft
h
ed
e
r
m
i
s
. There a
r
en
e
c
r
o
t
i
c areas with
colony o
f organisms i
nt
h
ec
e
n
t
e
r
. The l
e
s
i
o
ni
s surround by i
n
f
i
l
t
r
a
t
i
o
no
f
n
e
u
t
r
o
p
h
i
landgranulart
i
s
s
u
e
. HEs
t
a
i
n，X 3
0
.
F
i
g
.4
. Abdominal s
k
i
n 7 days p
o
s
t
i
n
o
c
u
l
a
t
i
o
n (No. 2
)
. P
u
s
t
u
l
e
sa
r
e observed on t
h
e
epidermals
u
r
f
a
c
eo
ft
h
elowerabdominals
k
i
n
. HEs
t
a
i
n，X 6
0
.
Stα
phylococcus hyicusのブタへの感染実験
清水
晃・岡田幸助・河野潤一・寺西
木村
永
重・杉原一二
要約
S
t
αp
h
y
l
o
c
o
c
c
u
s!
りn
c
u
sのブタに対する病原性について検討した。ブタ穆出性表皮炎から分離された
S
.h
y
i
c
u
s菌株(ファージ型 89/839/8188 と 89/8188の 2株)の培養菌液を、 3
0日齢のプタ 3頭に皮下接
種あるいは皮膚擦過接種して感染実験を行ったところ、 3頭いずれも接種部位に自然発生例と同様の接出性皮
膚病変が形成された口しかし、その皮膚病変像は自然発生例のそれに比べると軽度であった口病理組織学には、
皮膚の表皮表層に球菌塊を伴う細胞崩壊物の堆積が認められた。以上のことより、
皮炎との関連が示唆された口
S
.!
りn
c
u
sとブタの海出性
2
1
2
A.SHIMIZU，K.OKADA，J
.KAWANO，H.TEHANISHI，S
.KIMUHA and K.SUGIHAHA
ぬ)ザ帯構
..へ ""r~ 穐込J
γ事
態 :
w
で
tf-

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