オリゴヌクレオチド d(T)2-20のLC/MS分析 LC/MS analysis of oligonucleotides d(T)2-20 F060118A Oligodeoxythymidylic acid [d(T)2-20] A) UV 100 T20 T10 Diode Aray 269 nm 5.50e4 % T2 0 0 5 10 15 20 min B) MS 100 Scan ESTIC 7.50e7 T10 T20 T2 % 0 0 5 Column 10 15 20 min : Hydrosphere C18 (3 mm, 12 nm) 50 X 2.0 mmI.D. Eluent : A) 5 mM di-n-butylamine - acetic acid (pH 6.0) B) 5 mM di-n-butylamine - acetic acid (pH 6.0) / methanol (20/80) 42-70%B (0-20 min) Flow rate : 0.2 mL/min Temperature : 35 °C Detection : A) UV at 269 nm B) ESI negative-mode Injection : 5 mL (5 nmol/mL)
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