" #$ %&'()*+,-,./0 12 3456-,7! 89:;<=! ! !"#$%&'()*+,-*./0&'123!"$%45! 167389! >! ?@ABC! DEAFG! HI JK+L-+*+M N985-8537! BOPQROSTUV!VW!V E-mail: [email protected] ! !"123:;<=*>?1@A&'BCD3EFGHIJKHLJMNOPQRS0TUVW3X !"*#$%&'0YZ[VWZD3\I]^M_`Pteris vittataa@!":;<=*>?1bCc de&'*fghi3X:;j*!"@!kGlO`As(V)a0YZ\I]^M_1mnoW3p0 P[VWZD3PA:;jh*!"k?q*rs'*tu0v*QR@wxyVz1oWZDeDX {|@AQ}Se!"<=~•MK€•0YZA\I]^M_0!"k?rs'*‚ƒ„C123# Q}!"EFGHIJKHLJMNO…*5†B‡S0YZ89Bˆ‰ZD3X ! 1.! @ Š ‰ 1 2.! ‹ Œ • Ž ! XYZ([\]^_Z`abcdeafagh^i ! ÆÇlZö÷^_ø°^‹«ùXÕgXYqrú» j4kZlmbcdnopXYqrst\cu_vw ^_i1 û:8ü‹•pXY–ý‚ 1260 µg »¤‡c- xyz{\|}~•€•‚ƒ6„…_†‡[1]ˆ&'k \þÿ^i¶ÅÆÇph!bcdâ()þÿph!\ Zi2011 4 3 ‰ 11 &\Š(^‹ŒJK•Ž••‘’• c‡lmTXYpÚ¨g"#‹«â()p ¯$ú»^ “”\c•iXYg–—˜™š›œp•žŸXYg– _iXY–ýp¥†%lmgÆÇlp 5&.w/w7'ê — ¡š›)\cu_i¢o£¤lmTpXY¥¦§ ^‹«îï()*.+ ,-7bcdV,-iv,-. ¨‚©ª„…‹«¬pc-¤XY\c‡lmqrp® \ÆÇl»¶Å•{Mp/0g12^i Þ…3…p4 ¨\Zi¯)g°†‡±²³8´;<8µ;¶·¸‚ XY–ýg56^‹«Ò‹iÆÇlTpn7aXY b ¹º£»¼½¾…‡«¿\iXYp¥À›¯)»^_ cdÞ…\–Ò…‡ As(III)gÞ…3…56^ilmT Á¾…_†‡Â´ÃĶÅ.Pteris vittata7ZÆÇŽÈ n7aXYpØÙ8p 9*Ú¨g"#‹«XYÕ¨\ ‚©0„…_†‡¬»É¾iÊË\Ì°ÍΤ¯)p èéì‡â()Ziâ():;pùXÕÕ¨<Y=> ÏÐkч[2]«¬…ÒkpÓÔ\cu_ilmTpX ? aroA g@A»^_"#‹«¶Åàá/0ɾBC^ YZXÕ As(V)»^_´ÃĶÅ\Öׄ…‡¬» ‹4 DNA gDE»^_iùXÕÕ¨<Y=>? aroA ‚Á¾…_†‡[3]«^É^ilmTpXYØÙpÚÛ g]F»ì‡Gµ³ÄHI:8 [4]g°†_iPCR-DNA ‚¯)ÜÝ\cu_¤„…‡pÉiÞ…»ßlmàá µ³JµKHpLM» PCR-tRFLP NOgíu‹« pâ()\cu_¤„…‡pÉZÁ¾…_†¤†«' ÓÔZiXY¥À›¯)kч´ÃĶÅg°†‹ 3.! • R 0 T • XYp±²³8´;<8µ;¶·¸È\c‡®¨ãä \åæ^iÞ…\c‡XYÖ×AÀ›\bç‡â() (1) ´ÃĶÅ\c‡XYpÀ› pèép§ê†gë¾É\쇬»g棻^_íu ! îï()v,-.plmT 4XY–ýg56^‹» ‹«¶ÅpÆÇîïðg°†_i´ÃĶÅ\c‡ ¬Piîï AiB kZîï()*»QRÚ¨‚¤Éu XYÀ›p¿ñgë¾É\쇻»ß\i¬pXY¥ ‹‚iîï CiD.SîïTUZVV\5W7\b†_ À›\]ì‡â()èépÍÎa\І_òógíu ZiÞ…3…()*pXY4ýp 48%» 60&ÒkXY ‹ôºgõE쇫 ^‹¬»‚ZÉu‹.VV7«ÏŽiÆÇ()v,-. p´ÃĶÅZiV[ü‹• 334-406 µg pXYgÀ ! 26 ! "#$%&'()*+$,%+-./01-2345 ô^?õGFSöds÷£Hïð'øH, .6789:;<=>?@ABCDE"*+FGH% &'I-1&JK$,L$2M3NOP#$QR D . =>82OP#FGJGQR C .=>@AS67.E "'T1K$, (2) 345UVW67.XYZ[ 345.UVW67N\6] As(III)&6] As(V)? ^_#F`a#$/02QRbcdefgh2345 .UVW\6]8ijJkJHLhlm#$%&'( )*+$nop(I)q,UVW.6]82QRbcr1st*+2ufgd?vGF82QR A2B h8wx 1Jlm'yz*+$'2QR C2D h8{|Jlm 'yz*+$nop(II)q,%.%&1-2Z}*+$6 oe! ëìQR?vœH34v@˜9:;<=>. ]'=>?@KFBC*+$%&'~k•€*+$, •67‚ƒ.»rZ[ QRbcr&ufdh2QR A2B .•67‚ƒ. Z['ij„-+JG'noeq2UVW67.…no (I) pq'†‡klm#$%&1-2OP#$\6]8ˆ V[*+$%&'•€*+$,L$2\6]@AV‰ Š.‹G6]'QRbcr1-st*+$%&1-2 OP#$\6]87Œk6]?Z}*+2*-?ˆV [#$%&'•Ž-+H, (3) =>••‘’.\6]][“7”•–.‰— 67.XYZ[v@˜=>™.š›?vœH•žŸ . ¡N¢‰£H$¤2=>•• DNA 5?¥¦£H\ 6]][“7”•– aroA .‰—N›K$,PCR-§¨© (II) ª‰—./02M3NOP#$QR B2D h.« aroA . PCR ¬Ÿ'()h‡2-®Ws¯./02Firmicutes v@˜°-2±-2²-Proteobacteria ?³£H•žŸ‘’ . aroA ”•–´µ'¶·kst*+$,L$2t-RFLP ‰—?@A2%+-. aroA ”•–N¸¹£H•žŸ. º-82rg.»¼&&S?Z[#$, 4.! ! " # $ % o 2! ëì345.UVW\6]ƒ(I)v@˜UVW 6]ƒ(II).rZ[ ! ½¾82¿ÀÁÂÃÄÅÆÁÇÈÉ?@KFÊ‡Ë %*+$·GÌÍ.3467ÎÏ?Ð#F2Ña.6 7ÒE"=>ÓŸNÔG$67Õ[Ö×NØÔ£H$ ! &'()! ¤.ÙÚNbc#$,9:;<=>.@ÛJÜÝÞ< [1] Nordstom. D.K.: Science, 296, 2143-2145 (2000) ߃.†‡G=>àÓŸ.ØÔ82·ÌÍ.67ÎÏ [2] Ma, L.Q., et al.: Nature, 409, 579 (2001) áâ?¹ã&•Ž-+H'2ãäå67áâÖ×.Ø [3] Huang, Y., et al : Planta, 243, 1275-1284 (2011) Ô?8267ÒE"=>àÓŸ&\6]][•žŸ& [4] Hamamura. N. et al.: Environ. Microbiol. 11, 421-431 .žŸ-æçÔNI-1?#$ÛŽh2è.-æçÔ (2009) N~[*éH%&Jê.=>ëìÂí.(î'ïðñ &òw+H,L$=>?E"#$67.ó•Jô¢D ! 27 !
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