PDF file - 東北大学

FBC Lett., No. 30 (2009)
2009 6 ' 生命化学研究との出会いそれから．．．
東北大学多元物質科学研究所
永次
史
' '人工核酸を用いたポリメラーゼ反応
～人工核酸アプタマー創出に向けて～
群馬大学大学院工学研究科
桒原正靖
'シャペロニンを利用した機能性物質の創製
東北大学多元物質科学研究所
金原
数
'光誘起電子移動に基づく蛍光プローブの精密開発
東京大学大学院薬学研究科
浦野泰照
九州大学大学院システム生命科学府
戸井田力
' 大阪大学大学院理学研究科
町田慎之介
東京医科歯科大学生体材料工学研究所
森谷優貴
' IIS 型制限酵素を利用した全自動プラスミドコンストラクション
北海道大学電子科学研究所
'
小寺一平
%#!$
2nd Switzerland-Japan Biomolecular Chemistry Symposium (SJBCS2009)／国
際バイオ EXPO 特別講演セッション／第 24 回生体機能関連化学シンポジウム・第 12
回バイオテクノロジー部会シンポジウム／FCCA セミナー FCCA グライコサイエン
ス若手フォーラム 2009
' &"&
受賞・会員異動のお知らせ
編集後記
t&6{~ąøĈ¸ ¸ No.30 (2009 June) 2
¸ `26ć/{Üje×Íº'Ýt&6{~ąøĈÜ=²Ü+Üdî¿ÑÒÅ¯>Û
a×ÍºÂî¿ÑÒ¿ÖáÎ¹¤ÛZÃëÑ=²îäÀAâyËÖ¿ÇØ¹ÙÜ=²Ãèä¹
xcÜt&6{~Û¬Íê1¹áÑ_{~ÜYèËÊîMÌèë¹+Û®Ë¯>ÛĀąùöăĈ
îMÌáËÑºØt&6{~ØÜÃÃíéÛÕ¿ÖÁÑXÛ¹ÉÜ{~ÛÊëÖ¿êW»Ø
Ü¿Ä2ÅÃÔÑÉØîRãÖJ¿Ë¹}Øt&6{~ØÜ¿ÛÕ¿ÖZÃÏÖ¿ÑÒÇ
ÉØÛËáËÑºĉÜ'³©ÜçÀÚ8×uË¾éáÏïĊOîÞäØ¿ÖâáÍØ¹1995 ?Û¹{
~7Ü ×¾êÊïĉ2@26ĊÛÇÔÕ¿Ö¹ãÖ(×Ü Bioorganic¸ Chemistry ÜõĈ
üĆóĆþðąĆ÷ÛËÑXÛ¹s)¹t&6{~ÜHØÚèëÖ¿êtWØÂ¿ËÑØJ
¿áÍºDX¹b§Û¬Íê{~î4ãÖČ?Ä¤ÆÉÜûĈā×-SîZ¿Ö¿áËÑÄ¹áÒá
Ò¡×\d#P6Ü±*îÁê{~ÄÛ×ÅêÜÃÙÀÃ¹2.Lï×¿áËÑºÓæÀ
ÙÐÜXÛ¹ÊïÃèíëËÑóĆþðąĆ÷×Ý¿{~ÜW»Ä2.ñôûòÿ×¾é¹
ÐÜDX}ÄMÌÖ¿Ñ¼±*Ü,½ÜçÀÚäÜÝáÔÑÇÚÇ¹t&6{~ÛÕ¿ÖmwÛÍê
Üî¿Ö¹áÊÛzÃèÀìÉ¹×ËÑºÉÜóĆþðąĆ÷Û×ÅÑÉØÝ¹EÜ}Ü{~tî2Å
Ç.ÁêÉØÛÚÔÑØJ¿áÍºĉ×ÍÄ¹0¹9×´âÍÆÖ¹eÜ]¹näÅÃÎÛ¹Ø¿Àç
éäÈêrN×ÝÚÇÊïØýĈĂúĄĈÜ^Ü×iîÜâÚÄèBÔÖ¿ÑÜä¿¿J¿×
ÍºĊÐÜE¹p¹-"î
F×Å¹óĆþðąĆ÷×!ÈÑoîÛ¹¦
5GÛ$ÈÑ{~î
;ªËÖ¿ÑÉì¹ÊïÃè¹_ÄwÍêÅÔÃÈØÚÔÑq_×ÜÜÂîÂÅË¹ä
äÚÇËÑ¿ØuËáËÑºØÉìÄ¹DXÝ3K×Ü|fĉ9lT¨Ä·ÇÚêĊØ¿À
Ñ¿ÂÛ¹îUIËÑOÄ¾éáÍºt&6{~ÄgCÛwËÖ¹ċ'¹2×
íëÑt&6{~ÛÊïÛÔÖ¿ÑÒÅ¹3K×ÊÏÖ¿ÑÒÅáËÑº¢Ý£¿
äÜÜ¹č?çé:ËÝ{~Ä¢â¹åÔØ¦
5GÛÁÐÀÚ¥ĉ<dKb§ĊÜµ
ÛÂÈêdîßÖ¿Ñ°×ËÑÄ¹ÉÜ×ä¿ì¿ìÚtWØÂîÊÏÖ¿ÑÒ¿ÑÉØîO
ËÖ¿áÍºÐÜE¹v6ÃèQé¹2003 ?Ût&6{~Û%ï×¿ÑÒÅ¹wÊÏÖ¿ÑÒÅáË
ÑºÉÜXä¿ÕäÜçÀÛÄk¶Ë¹[EÜnÒÔÑVvÊïÜwX«ÄàØïÙhÔÖ¿Ú
P#Y]½¸¾ No.30 (2009 June) 3
£r8¨P#Y]£±°@¥P#£&~-£M5
´;¢¤ £°¡ «J´«®¨2006 . 4 D£*!#®E!#£
¯Cx¬¡j¤Y]$ 9"´
bha v§°¶·¹¼´^¯¨
>.!# R¤Y]´¬°¡¤}´7¨[¤Y]¥{"SN´2
°?+"´9±´Qt`bh ¤s´l¢¡¤¢Y]¤
K±¢¤ #Pµ¥u\¤Â.|¡XB| ¤¢¹¾»£¯d©¨«¤
¯¿9À£¥°\/¤:m¡Ww3n °ª£¢¢VT¤«¤9 G
°²£¬¡9c°¡¡
¯¨¡?#Pµ£Iis
®l¢«®¡¤¥}f(9®·¸¾º°ª¨Á.¯¨=g¤oL
®«R ¥1°¡«
#Pµ¤Opy¢¡²
¯=g
z_£¢°¤ ¥¢¡5¡«¯¨
j¤Y]$´;Ã.eA6©¥'¢¡² P#Y] UH£k´
;°¢Y]9F´V<±®«0¯¡5¨,q¡¥¦³
¢%£¢¨¨±®«P#Y]¤¨¨¤S)´Z4¨
ogx¥§M:Q No.30 (2009 June) 4
* ! $/312 5 , :
+.-05&"%:
[email protected]
8(#)&
W}¿-ºa'
»ºakW}¿-JO;G71KQAO2!*T
À¶´*¿JO;G71KQAO2SELEX (Systematic Evolution of
Ligand by Exponential Enrichment)1,2 & Non-SELEX 8M15IO 3 SÄ8M15IO 4 )[ÅY±-%¿.D:FQ-X cvÇ:Q4=?È|eµ*
*ÇpÉÈ¿.D:FQ-*eb*
*&g
xl'RËÊ73QLy]µ¼*
U~^w*Â
*
$¥§¹²&h²P¸²!
+
*¿B1M.Q9Ç¿c·¾¬È
'[`{c·+#
z
gu*¨¯(h²P¸
²©!-¡¿sq½&«½KO¿
½-gx_Æ*'B1M.Q9°
-mTW}¿.D:FQ-\µ*´
Ã-³*
8
*!$/3125,
w¤'¿B1M0<@,+*q
iZ
KO¿6/7>Ll¢d®
c
v*Çp 2ÈB1M0<@sq½Z«½Z
KO¿½Z().O<8O7h²&¿DNQ
C!&VÀÁ·-¡ +(½Z-gx _ÆPt¦
W}¿
rn+¥§+
(½Z!_Æ
EKHJQ9kj"f
£¡EKHJQ9'*W}¿ºa'
»ºaª
9 64 7'
b\mD6F No.30 (2009 June) 5
3
' hf»N
SÁ_
(+)7@E
O
O
&393E=C@4Ehf 5 N/8;E&
2/;E>CEhf 7 N
O
NH
N
O
¤fsU
r?CABF5¶qp*(+
*y+(»N«Tf.sU<1
NH
N
O
O
O
O P O P O P O
O
O
O
O
TTP analog
OH
D03:HCE½±sP½«T³\°ª
v´+*Ãd 3ÄG§+(°ªk
O
O3S
SO3H
N
N
efµHCE½ÃdATPdGTPdCTPTTPÄW
lI*$«Tf DNA ¾Lºx~
S
U+**R«T³ TTP O
NH
±sP. 4 Àke dNTP WgaT NH2
U+*!,ke T U)SÁ
e T U)**«T³\
«TfU~n"%Vc¿
. SELEX
iJ¼a*y SELEX
z*gaj
N
O
O
O
O P O P O P O
O
O
O
N
O
N
dATP analog
OH
4 '
./'
z*ga
*SÁefµHCE½rz*kefµHCE½?CABF5]z.¬"
)TTP ±sP.Q*ke TTP .dATP ±sP.Q*ke
dATP .]z.¬+T U+*!,VSÁe T A U+*!,VSÁe A U+**
(
4 ÀkefµHCE½SÁefµ
HCE½.Z*Y·!
?CABF5]z[jOI
*
¥(SÁf*
SÁefµHCE½.a}+(.
?CABF5]z.£¯
*']zº\.°#
¢SÁf\m¹&?CABF5
À"+(¡#a-]
5 #+.,-0*&($
z^&{ow.ju`*
X
?CABF5À
_ © Ã Thermococcus
kodakaraensisÄ¦| DNA ?CA
BF5ÃKOD DashÄSÁfµ
rrz*Mt½.Â{ow
^¨K*X
(¥(?CABF5'*S
Á DNA }]z.¸w²®*
6 !12)"%+.,-0*
´qh¹½M<P No.30 (2009 June) 6
*kß¼
l³)é1j$-1À¤Ä¢]ë
w
DNA Yæå¾ZÆ-FLIKP;Ð|g³
É[S-]
ëw
ävå`ZÆ-Ð|g³%,[S
dît 4ïÄ¢
z±v DNA 1äv]ë DNA 1´-kî×cï*,(]ë DNA 1ävz
±v DNA 1´-kîÚ×cï
--1»-ît 5ï
( ÁáZ1]ëU¥ã1µFLI
KP;kVTÂa,Àf·Ë0.
-*KO=H:6LPBO7®
#µ)×cî!ÇÑï9:?H
FLIKP;k«¨Ï
¸·-¿È+-Á
áZ]ë3AN7²¥ãiÊ#
µ
Î.-£ªv¥ã (BNA:
Bridged Nucleic Acid) 7 1µFLIKP;
) &#'"$%
k1¦Òì£ªvC6M59
@RLOãÔ\îKTP, LTP, MTPï*"
£ªvC6M5>@îKLMKA ï1p
'ävå1µît 6ïÄ¢KTP
1µxn 3 wLTP 2 w
MTP 0 1 wYæÍ+.
ît 7ïQ£ªvC6M5>@
1p'ävåk1Ë/£ªv
* &#'"$%!
C6M5>@
ÝÅ~u-ävå
5ñ9 w¼Yæ
_¬
ð- 2 3 £ªv
C6M5>@1a(1ävåµ
/KOD Dash DNA FLIKP;
*"y¶\1µ.
<4E L £
ªvC6M5>@1èk
%Þ
ËbæYæå
ºÓ.ît 8ï
+ oàX}ÞhD2GLPB -
Vent(exo-) DNA FLIKP;(o©Yæ
ºÓ.
ÜÏ¡*,eÙâÃ*,(Õm
,Ø&îG:4O8PFMP9JOï1´)
»r.
U¥ã1µê¹½
Û 50 ^1Ö-íâÃ°)ðç1
Ö-ækç§¾ W
µ+.-
-¯1Ì
-s&
ìkç 5 dçâÃ°{° 10 ^Ë
µv½À]I` No.30 (2009 June) 7
'$(FCD6" [_ÝÛl%qæ$&SES^J=;`
L=[BO@]=JMêPS-ODNë.TZ
PQ<EQ :`L =[BO @]=JM
êBH3--ODNë#"2ê 9ë2
O@]=CMb[_Ý4¶3'ÜÆº
$30%h¦Ý4ÔÏ2!zÌ
2 930%h¦Ý4¶7_J
G_E.7RIV`#"%ªÌ¦Ý4n
Ï2{''-[_Ý%?Z[K
8å!#2sº$ÅÃ#7N^A
-â¹32èp
I# .8/84CD"#
2SE
S^J=;`L-TZPQ<EQ:`L[_ÝD;EK\Óm
2%i$-¿
%È{
%Ûl4É h¦Ý¡
2³$®Õº{¤#7WMÈ{.7W_È{#"
É =[BO@]=JM&¼
Î¦ÝêDNARNAë!%²%+#0%gÞà¨×êNMRë$-ÒÇ$?YZ
@IZ9F32DNA àf%30%È{U[XZ`Hy$x*ä$
&¬"~o#
ÁË0&RZ9V`à.ßà%[_ÝD;EK\È{4
7WMÈ{2&7W_È{$É ODN 4{304¶U[XZ`Hy4§
Ñê 9ë[_ÝÛl$230%É %t&Ûlqæ.ÄÛlqæ/1-
!káy4KOD(exo-). Vent(exo-) DNA
U[XZ`H&ßàc%tÉ 4ÖÙká
à4µ#0Ûlqæ.ÄÛlq
æ!¸#2±&ÜÆÖÙ-ßàc%
tÉ &d¯%káy4°!
2
10 $%ao4¾ÜÆ$ KOD(exo-)2&
Vent(exo-)4¶{7WM[_>`4 1 },
ßàíêë/(îêë &É tÂu

%kár«&)!5"Ð03#%$7WM
[_>`4 2 },ßàïêë
tÂu
&1 ·»%É
)!5"ká&r«# 2 ·
»%tÂ%u
ðìòñìé-%kár«Ð
03w3& DNA U[XZ`H 2 ·»%7
WM[_>`c4ÖÙ2ã1 ·»%7WM[_
>`%káy4°2!¾
2¢´ 10 $/2!¥É 4 1 t2!$/
2 DNA gÞà%Í±vêTMë&-0.1ò+0.9
2%
É t$/2gÞà%ev&)!5"
#!Ê032a£|©%ç4ÚjØvQ6
W[`A % Taq DNA U[XZ`H4¶{
&
HG# ;@>CD<E:9:
5-1?CABE=#
J*K7;@>6:0,!7 ) !+7 37+
376 &% 321,#
²spº½O<Q No.30 (2009 June) 8
"çwè÷øùôõ)Éxb¿"k[é`¤Ó,/.
gÅ
ª¸&#çwè÷ùôõ1¨Ú1 ´¸"É
xb¿"kkË#Â 45ó"[é`¤"!Ë#Â 75ó)"[é`¤
Ó,//# 2 ´¸"bÉxS«"[éq1¬.1»t.
X Ç¢ÞÔ,# DNA FMILQ; 5 ¦x)T«"Éxbã\1Õ×.
#Êï,/# 2 ´¸"Éxb!+çwèc]!),/.o·o"{
p[éq!Uj!a(#DL3GQè/çwèÒr],Ôî DNA FMI
LQ;e%Òr]!Zr.q1bÉx}.(Ê.
çwè"EJ8Q6P9ôT "ßÆähõV!Éx1b.DNA FMILQ;"9M
=DðÈ!Ó,/,DL3GQè/çwèÒr]DNA FMILQ;ë"¹W^³!
MPæx#¥Ù"¥"+
l1.")/
±vDNA FMIL
Q;"{µ]1³¢Þ£Î"¹ì!À .
/'!¾Ë,#yxã1_ñX¡æ"L3CLM1³ SELEX ©!+82
MNL4?Q9*7M@G3@Ö]5N<HPæ
1mf
11-13
!¯µ·!År.X¡æ2D<GQ
/,"X¡æ2D<GQ#"®Ø"²]d*ÄÍd!.o*l
1º½.("³
g~>QN/.
/'"º½Å1Ê.FMILQ;qÁã\*MPæã\1_ñX¡æ
1åÃ·!r.#Y"0ïi/.ôu 5Aõ
/,"X¡æ1Ý"
SELEX ©!â³¡æ2D<GQmÑ1u."#'-±·#
ôu 11Aõ
RKOD Dash +% KOD "{µ]1³/$/,"X¡æ1çw|w DNA
"¹Ðè1n°+åÃ·!r.gôu 5BõÛSELEX ©1³
2D<GQ"94MQAP5©ôNon-SELEX :O48KP©öR§í:O48KP©
¶//,"©#:O48KP"ÜVá¼¡æg~1z.^
X¡æ1åÃ·!r.Ï¼
õê
(
ôu 11Bõ±v¾Ë,#/,"©!+yx
ã_ñ+-,!B4O2Q;Ì"òX¡æ2D<GQ"ê¶1à(.
¡æ
¦d\n¯µJ9M No.30 (2009 June) 9
Ó/C9DM!?3J/M8¹!cN»z"R}'ÔÄÆÊÞ
,k{W
mÉÍ».'Ó/C9DM.rÙ S,»Wmr§\,Náz
.'QxÓ"ljâY¬
,G0BGH!73HM>K4!Ö«
Ó!£Yn¬oq\1@I2M(Wm:0=E37
)Ðs¼f
ZQx
hÑ»!®×;M9!Û´
QxÓ/C9DM.ÇÅLXÂ]ªßeÁ¦¥ih°¯µ
WÕ$vÖ§-,|-,
¯µ.Ò&,
+¤¶¿Q³nÀ½äJSTå©¬XÑ¯µÒOTQg
¯µäPRESTOå
Ñ»Å W)+kl,~.`%%,~Ý
*#
~[Y.Ï+%¸àlnbÈ ¡P p U¦)#aln uw y U
¦lØlnbÈ RÃ U¦aln tÎ º U¦
)+^~±¨N%
1.
A.D. Ellington, J.W. Szostak, Nature, 346, 818 (1990).
2.
C. Tuerk, L. Gold, Science, 249, 505 (1990).
3.
M. Berezovski, M. Musheev, A. Drabovich, S. N. Krylov, J. Am. Chem. Soc., 128, 1410 (2006).
4.
A. Nitsche, A. Kurth, A. Dunkhorst, O. Pänke, H. Sielaff, W. Junge, D. Muth, F. Scheller, W. Stöcklein, C. Dahmen, G. Pauli,
A. Kage, BMC Biotechnol., 7, 48 (2007).
5.
M. Kuwahara, J. Nagashima, M. Hasegawa, T. Tamura, R. Kitagata, K. Hanawa, S. Hososhima, T. Kasamatsu, H. Ozaki, H.
Sawai, Nucleic Acids Res., 34, 5383 (2006).
6.
M. Kuwahara, S. Obika, J. Nagashima, Y. Ohta, Y. Suto, H. Ozaki, H. Sawai, T. Imanishi, Nucleic Acids Res., 36, 4257 (2008).
7.
S. Obika, D. Nanbu, Y. Hari, K. Morio, Y. In, T. Ishida, T. Imanishi, Tetrahedron Lett. 38, 8735 (1997).
8.
M. Kuwahara, H. Takeshima, J. Nagashima, S. Minezaki, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 17, 3782 (2009).
9.
¡Ppæ_Üæ6AF5E7<HMÌË²æ×VÚLã¾¢· 157 (2003).
10.
S. M. Freier, K. H. Altmann, Nucleic Acids Res., 25, 4429 (1997).
11.
M.M. Masud, M. Kuwahara, H. Ozaki, H. Sawai, Bioorg. Med. Chem., 12, 1111 (2004).
12.
A. Shoji, M. Kuwahara, H. Ozaki, H. Sawai, J. Am. Chem. Soc., 129, 1456 (2007).
13.
K. Ohsawa, T. Kasamatsu, J. Nagashima, K. Hanawa, M. Kuwahara, H. Ozaki, H. Sawai, Anal. Sci., 24, 167 (2008).
V!.^aÓ¾Ö No.30 (2009 June) 10
2546371(*,
×[email protected]Ø
98 -)/+
¾ÕÉ¶r¢IKHv°B¯¯Rm¡cHv %Jk°B®
¡«Mh¯HvJk°;W®1P¢7«ÆÈ½¸ÖÒ¡Jk8
HvP°®:¢¼ÎÌÔÇÕ
£¯®dQ¾ÕÉ¶r°WJk8Pr
¡|Z°oC_¢¡ÀÏÖËQ¾ÕÉ¶r°WJk8Pr¡|Z g
®
:8 254637
¼ÎÌÔÇÕ×chaperoninØ¢y dQ¡b,°B®dQ®¢T
Q¡2)¾ÕÉ¶r~V
+JG¡]¬¯®[1]
)lnXE¡GroEL¡&7¡»Ë
ÐÇÁÄ×60 kDaØÅÖÆÁÂ
Q¡UQ~°¬ ¯¬
`§z¿ËÒÃÁ´Ö$¡
dHv°49®d¡¢
e14 nm(6ªe14 nm* ¢
64.5 nm x ® 2 ) b , 3
9 254637 !%$ " '0. !%$ "&!%$ # ,
®×"ÙØ+{¢840 kD
¾ÕÉ¶r¡¢)y 0®fj¼ÎÌÔÇÕ¢'8¾ÕÉ¶r¡ÑÊ³Ö
ÒÃ²Õ·°;®]¬¯®¼ÎÌÔÇÕ¡b,
u¢YL8'8 «YL¾ÕÉ¶r°/@ =¬®®¥¡5°Fº¼ÎÌ
ÔÕGroES¡-#ATP°N®dHv'°s'8¾ÕÉ¶r¡ÑÊ³ÖÒÃ²Õ·
¬¤ b,¬¡ÑÑÖ½s®¼ÎÌÔÇÕ°D?<¡D? ¢q¬¯
[O¢ÙØ\64.5 nm2)ÆÈb} ¹½Ä°t¨®ÚØ ATP°®b,
¡4'P°>®ÚO®¡« JG[ ®2)Í½Ä+°
1[ 9®¢SAO¢kI¹½Ä°w¦©¡µÎÑ±¡p¼ÎÌ
ÔÇÕ°W«i
:@AY Y No.30 (2009 June) 11
-+"'$( [2]
Y 0G&-*nel%;g}mUNp6
sZrBq&Qns=p}
!8
eZi~ATPqzkl)
mb}`o^`Zn
]^cnq\}[icmZvh(xqZ(Wpbdr
DCdS
elZDr
}nnyqZATPq%flD)
g}Z>D
MrPLOwj[CdSCr
DsK
6 !0+2(35')$*3#
3!0+2(3.4"
&,g}jxZqz}|SwZ)

/
g}ra+m\}[9
qCdSIRqs25&aun
a)
b)
op]jxZ`
{)
d~jqs#q
c)
31nelEqV`~}c
na'd~}jxZ
nelTel]}nF_j[v
hZ25&5m\}
20 nm
mPLej
CdSD?$2.2 nmZ
Tris-HClpH 7.5
rHJ<.tqX"
7J<.
50 nm
- T.th. cpn,CdS )!* *
&%# T.th. cpn
GroEL, T.th. cpnn4eZ
7 /(%4 T.th. cpn5CdS ')2,& 1-a) ATP b)ATP Q!+WYÊ²Ív v No.30 (2009 June) 12
¬É¡q]dx^>wTEMÎ"ÓÏ}ª
a
ËÂ·«Ç¾¥ÍÎ"ÔÏVUÆÀËºÌÐ¹
a)
HO2C
trans
N
Photo Gate
»\(e
4 ¢XixwÆ
N
N
cis
N
Photo Gate
CO2H
UV Light
( = 350 ± 10 nm)
ÀËºÌ*#z=CdS¹»\(®Å³ÁÉ
GroEL
Visible Light
( > 400 nm)
GroEL
Ä¤Ã¸KH¢¼´¾£ÍL2GDQ
x4 e
NU,-ywGroELe
50 °CwT.th. cpne
80 °Cnw
b
cis
b)
Open
GroEL
&7wg¹»\(:l~x
[1,1]-state
J1wÁ¯·ÆÀËºÌbNJ1.6
+ ATP & UV
trans
}we
,-7ÆÀËºÌ,-7
Fz~xw{, Æ À Ë º Ì /CdS ¹ » \ ( e y w
Tris-HClÎpH 7.5Ï¼´¾£ÍEKHATPwMg2+¦¨Ìw
trans
+ ATP
Closed
GroEL
+ UV
cis
[0,0]-state
Open
GroEL
Closed
GroEL
[1,0]-state
[0,1]-state
+
K ¦¨Ì¢I¡w CdS¹»\(
2: ¢fxtwÁ¯·Æ
ÀËºÌ@o¢z~x
E %!5:<A9A0'&!5:<A9A
7?=@;A0/*-D06
>B8
'
{:l=wATPwMg2+¦¨ÌwK+¦¨Ì
a*#yw Q
ÆÀ
ËºÌ&7²Ì½ªkÈ¾§ÍÉ¶¥Ì«¢p
=
Ayx{wÆÀËºÌ0O
.&7²Ì½ªk.
AB@w
¬¯·mw:¢ATP5
~x
DC7?=@;A0/12 [3]
v ÆÀËºÌATPwOkmw:¢
5wBc7Ok©ÆÈ¤ÍR¢`
|wOPUM5{'zx
w ATP6[7¢<ÆÀËºÌ6
[7¢wBc5z?h
Î"ÕÏx
v GroELÓ¯µ¦ÌC%¢<zw
¢¤ÇºÌ_93wZ)
nÎÒÓÑSÏ¯µ¦ÌC%¢;/
&T
¢8x&T
.wÂÊ¦Ã¸r¢
<¤±¿Ì°Ìj/
¢Rw
6[7¬Í·¤±¿Ì°Ì¢/u
ÆÀËºÌ¢48xuÆÀËº
Ì&7GFP¢|¡wsQ$ÆÀËºÌ
A ¢m¢Xix
F #"$ 00'%!+3,3
D0' &!).4
(-'
a)8ikñÚô No.30 (2009 June) 13
ÊÙæóØó·p2]<µÁÃÞîóÖ¡ÂÔÖº#|]<µÁÃÔÖ¡ÂÞîóÖº³
]<WÈ¹¥³µÁÃ#fµdFÈD²£ÄÞîóÖ²·5¢Vf¤ÔÖ²
·Vfh¤´Ä¾p2#|]<µÁÃÑôÞ¶¦È#fµÒóÞòôð²£Ä¥³µ´Ä
w(Y¥³µ¥¶ÊÙæóØóÔëçòàóÈbÄ¥³²ÑÖÞ³§±b1FGFP¶J
AÈ]<µÁÃD²£Ä¥³¢¡¯õ+úö¼©ATP¶Bµ°±Q~§¢p2
#|©ÅÈ]<§0&µ¿ATP¶7-§´0&µ·ÑÖÞ¶JA·R¾±¥³¢
¡¯¥Åµ;§ATP7-p2È]<§±ÊÙæóØóÈÔÖµdF¨Ä³À¡
µ1FGFPÈJ§¥¥²#|È]<§±ÊÙæóØóÈÞîóÖµdF¨Ä³J
A¢x§¤¨Ä¥³¢¡¯¥ÅÂ¶qO·ATP³p2È$³§±{³£«¶ø
°¢ÂÅ³£µ¶½õ1FGFP¶À¡´Jö¢¥ÄAND`*¶Á´En³½´
ªÄNo·5SP¶
È`*µÁ¯±D²£¾±¶µ´Ä
$"'(%)! [4]
\¶e{²Ä¢ùµr¤ÔëçòàóSu¶Dµ¨Äik¶²8§1
dÔëçòàó¢?ÌÎó¶ZµÁÃ÷TfµK§Ûìôå_¶&ÈÄ¥³È{
®§«¶qOµ°±>§§¤»Ä
µ ±EnF³§±ÊÙæóØóÈb±¥Å·]<µÁÃÑôÞ¶^`
f´¦È3£¤1¦ªÄ¿¶²¯¥Åµ;§]<µÁÃRF¢3£¤1¨ÄäÍÞÏòéÜ
Ï5³§±ÖãòãîóÈ=¨Ä¥³Èt¼©4\.ÔëçòàóGroEL¶%ÓåíàÜÞ¶
ÔÖÝÌóÈ¨»±ÊîàóµsI§Lµ311cg¶ïÕó³314cg¶òÌÔóÈÔÖÝÌóµsI§
GroEL1d(+û)È
z§¥¶1d²·#u´ÔÖÝÌó¢}28m¶l6¶Ã
"µ7-¨Ä¥³µ´Ä¥¶Ôëçò
àóGroEL1dµ;§èñÌéß
ÈM¨ÄÖãòãîó=ÈZ
¨Ä¥³²õ+üöÔÖÝÌóU/µÖ
ãòãîóÈ=§Cp2#
14.6 nm
4.5 nm 13.7 nm
|'!ÖçÏÞðÈ[9§³¥ÆÖ
ãòãîó·N:o²b§±Ä
áÜäÉô²¢vefµêòÔ
Êàó.µdF§o²·@µÖ
+ GroEL ãòãîó³êòÔÊàó¢7§_
GµÄ¥³¢Ç¡¯

O
E¶j·ÓÌ×H.
,XÏòèÞÐîäËô(GPC)
N O
µÁÃy¯¥¥²ÚóâÏ¶'!
W²Ä280 nm Á¸Öãòãîó
O
.5¶'!W²Ä350 nmL
µêòÔÊàó.5¶eW²
Ä654 nm ( 381 nm)²«Å¬
N
O
365 nm
NO2
> 400 nm
N
O
N
NO2
O
, ' &(*#'(
5 9<§ªa a No.30 (2009 June) 14
J a)29B9ACG>B0+6C0?;B6C"</70-=* (EF%$EF#.B<4/
A=9C.K&" EDTA *!#.B<4/A=b) "%'&)!</70-=
#EF%$EF
IH 0?;B6C583@D:# ,>D1
¨ ¥¡3!ob
_*j|NBowemy:Josc
_'}$3!|b
GroEL7}©}hr³²«j
_nwemyjiusc
a _'}m}7GroEL| ¡2osymbGPC¨ ¥¡x`S,
}jAokos¬³cn|MO\^(V|b
_GroELj@#Z;obWe¤
ª4}j%,owemyEos(°¯)cI=|0Gos>/bm}f{¤ª4
}%,|~bq¨¥©®¢¨©|
_j)]xdmyjiuscn|2
pT"|vew0GBus>/b ¡tlx{kb¦¡U{z}±
}T"©}2j/8xdmyEetoscm}myib¤ª%,|~b¨
¥©®¢¨©PyT"©y}QjXowey?gcmmxb¤ª%
,osH.|EDTA2pyb¤ªjF[pmyjius¬³cm~bEDTAy}Q
| ¡©jYisxdy?gb¤ª}%,|hewQjR
D{&-uwemyClwec
a 1|b£¨©j+©KLmy6owb£¨©¤ª|
j%5tyëØî No.30 (2009 June) 15
:´Ê±¼ÎÄ·¢
Ãµ¡ÑÖmV¿/E
-lactalbumin Î éáê³ð¿<
ÐÒí¿4)³½§U
»¡×ßìßéíïå
ìÖÏÜíGroEL
¾©·¼±Ì¡2c
*GroEL¿-"¼#[
¾ÉÃËÊ±¼¬
33 a)!-lactalbumin #,&+'$-#%0,
)&/-b)!,&+'$-#-lactalbumin #%0
,)&/-4
.*(14-lactalbumin ".*(1
«¹·¢±¿eG¾.
ãÔÝÖÑäb³
· ¼ ± Ì ¡ / E -lactalbumin ¾ m V ´
Ê»æÜØî³·ÓìãÚÔéä¬¡|0$»æÜØî³·ÛÞÙèîà¿ÓìãÚÔéä¼½Ê
±¼¬«¹·¢´½Í¸¡/E -lactalbumin¬ÉÃË·ÃÃ¡ÛÞÙèîà>H¬±Ê±¼¬
«¹·¢±¿Ç¨¾ÖçâìÜí¿R1¿dC»¦ÊÕ×ÚÉÄÎk³ºÛÞÙèîà¿¾Õ×
Ú3ÎÉÃ¶Ê±¼¾H³·¢
2
ÖçâìÜí¡d¾GroELÀ¡QZ¬Os»¦É¡j¾ª¯Ê?¬}¾Y²Ëº§Ê·
Å¡[£F,Î9P¾AÊ±¼¬»Ê¢²È¾¡67E¬®ÉI§Æ_q9P»¦É¡1Î
¹·1piÆ!»¦Ê·Å¡[£½/oÎ1¾AÊ±¼¬»Ê¢'~³·/o

ï5¼§¨¿aË¾ÇÉ¡ÖçâìÜí¿Zqd¡ATPDzE¼§¹·f¿q½E
Î`«³·¡N³§q]TM¬!»¦Ê±¼Îu´±¼¬»·¢@À¡ÖçâìÜí¿
j&EÎ`«³·8kq½DkÂ¿;Î(¹º§·§¼©º§Ê¢
Sx»~³·9À¡W1515=5{tyw¿rlKJ¼Æ¾½¹·tyHX»´¢
Ã·¡#ty¿W=1515=5tyw¿hl^LjªÇÁW1515N
+Hw5tyw¿l
\j¾±¿-ÎÉºBvn³°Ã´¢
¤Lg¥
[1] Braig, K.; Otwinowski, Z.; Hegde, R.; Boisvert, D. C.; Joachimiak A.; Horwich A. L.; Sigler P. B.
Nature 1994, 371, 578-586.
[2] Ishii D.; Kinbara K.; Ishida Y.; Ishii N.; Okochi M.; Yohda M.; Aida T. Nature 2003, 423, 628–632.
[3] Muramatsu S.; Kinbara K.; Taguchi H.; Ishii N.; Aida T. J. Am. Chem. Soc. 2006, 128, 3764–3769.
[4] Shuvendu Biswas S.; Kinbara, K.; Oya N.; Ishii N.; Taguchi, H.; Aida T., J. Am. Chem. Soc. 2009, in
press.
¥sm|¬¯WL[ No.30 (2009 June) 16
+-1"(
%/
0'&#!
.
([email protected])
32
Ä¥s£Â5¾=¨»t5¾
324/
¥"/@¢5¥¡
¿4$@®5¥¤¡Ã5l
CUEVLFR4½(@%1!;
/Ë»0@%1!#ÀÁ&A@>
43-/@%5>3½C}£
(@1'/£v½ÂizCÐ
4p¼m(@¸fQX[PC¦/
¸fÎÌ]0¥²µ°C½(@
!#¦&A/@uÓ\4¸
fQX[P¸fÎÌC¦/¥"
/@²µC¥"+::½(@
5n¤C±4:1;+½Â
1(@¥¤izÑÒ5hC´
@1"81D25¥¤¡Ã6 ·
0@+;f|ÎÌ0+,½~'/
<*5l"C«@%16h3!
9 3462(-),.$*&:78 *
0153462 "! ,#015+%'/
8
6e6 ¸f0?1oZ³r(@%10j;/¸fC©(@izÑ¸fQX[PÒ
C²µg4{v&)@%105l"C¸f5xm1'/Ð
.UEVLFR4Æ%1Cp
¶1&)@<50@
b7FGY!²µg5w`ÈCq@_ºª3KHYNSMKYJT[1'/d/@%
164§5 ^}0@!%A6 FGYCÉª4¸fhp¶3¸fQX[P5Í©
4>@1%B!;/y"_ºª3 FGYh¸fQX[P0@ 5Ç1 FGY
h5n¤Cu ]4'+ 6OVGWKFYÏ1 FGYIW[L[0@ Êa
1!¹r'+Ç0@!OU[5¢0689 ¸f0?%A! FGY1³r(@%
10*5¸f! c4\(@+;FGYh¸fQX[P1'/¶(@ 42+-1"(
,%*)
$
\Å5>4¥²µ½4;/Ë»3kC+(¸fQX[P0@!%A:04Í©&A/
ÌÔÚh\l No.30 (2009 June) 17
(4·¾£ç~dilc>B:O;? ´¨9ÿÐ-L8(8%J¸E>¾äN
É/Kç~dilcNÇ68ÿÐ/K,:?¼F8Ă9!64,>ÊÎ:.8És>¶³>ü
íÞNĆx.8G³4<·¾Ä>ç~Åq9G3>µK-Ĉç~üÈĉN¿Ö=
rÂ/K,:?BCpä9!J3LI³ëÄõNā=®.ç~ÅNÂ.8EKD9~
K&~I<"&?M&I<",:'!+ILKH68,LD9}
?Ãç~9!J:¬k
á/K,:9F8ç~NÐ/Kç~dilcNÿÐ/K=?ðèþò=7),:'mÏ>øú
9!64',>´Á9?³4<»¡ôNì/Kç~dilcNÿÐ9(Kzï?)Ã)
ā,LD9=ÿÐ-L8(4ç~dilc>\lV]`?Ùý¥PRj<;Ą§=ĀIL4
G>9!64
3,9ÜãI?,>H#<ÆÀN±Õ.ÒÑ>¾äN·/Kç~dilcNóÊÑ=à =îí/
K,:NÒ½:.4ÔÚNè68(4áº~ñöăØ
NîíÊ:/Kç~dilc>óÊÑ<_XPjÁNÖÛ/K,:=®.4/<M5y$@s
éÑ<ç~9!KbgRhZPj?Nej[jËûu:ç~9!KSWj^jËûu>Ċ
ûu=*8â$K,:'ä9!J =HJ3>ç~ÅNà =«ä9!K,:N
ê.4Ĉ ¦nĉwÑ=?ej[jËûu> QagTlheg'!K|HJGć"bg
RhZPjñ¢w?8BCÃç~9!J,L'v"ñ¢w?8bgRhZPj:Ý>©"ç~
NÐ/K,:'µI&:<64ĈĊĉ<2mæ=rÂpä9!64ç~ÅN¯
NÍ"4îí9?rÂä:<64&Nß=ùAK<I@¯
>°Á
>°Á9?ç~9!KSWj
^jË=?){ąN$8%I0ç~:?ăª.8"<"ej[jËûu={ąN$8
"K4Fç~å÷>¹Ñ<Å?²<ML8"<",:'!+ILKā,>°Á=7)
îí9?3>kç~YfU`g?B:O;10üÈ>E'/K<;ç~
å÷>¹Ñ<Å?z4L8"K,:'µI&:<68"K
tn>ÓêNÐ¤-1K,:9ÜãI? ¦o=×.4ç~dilc>óÊÑîíÁ>ÖÛ=®
a1 'G//
,&E7MVZQ[SO]JaBI-H=D#8M<F9E:_`5?I&
.JY]T]-I 2434 PUZR^[YZH=@*,0IHLAD5"H
>M<F9%E7M_`6!$9<NKEH =@5*,0HC;&X\^WI+")(
_`61
ÁÊÍk]o No.30 (2009 June) 18
.3!/<L5
"JÛºá=/J×{glof>ìÆMÓ%Jï
1>Ûºá:¿
ÃÇ=Òn¥. '71>¥¡9â> PbjUokhj()*wr/J¥õ
{Ç<?p¤Ù<#ö-%É68#K@
,KM¹Â.8¥? =GIAB½×{9"
I ¥¡= (ã,H<*<J,:9#×{MÆ/JglofMàÀÇ=ìÆ/J,:(Ö:
<63!
*)$'(&!#"
¹¦êÐÌõö?
»Å
Sm<;*>Ä§=îLJ:-K
â:.8> F7:>®¯F"I
QTY`
æê¶Ð
K¿£Ç<¥¦M7,:'H
C3ä9?ÑÕ}¨vç¾
C/C/¸ÈMðE8#J!p= :Ü68F
cO`lTYjiZRj
%
pëóêÐ<;*>Ì(.
[odo
,KH?1K2
Áx}=&#8FÃ<J M7Ö¦Fô#! ´~Â
×{glof? ÎÔH>ÊÍu=F#*7'ìÆ-K s9FZVllejQk\Om>÷òé
zx9"J (*Â#HK8)3!.'.<(H =? í
>¿Ã¦?|*<* C3ã{M8J4+9 >²½=''LH0)*×{(.8.C$
µ¼M68&I
Á¾Ç=©"J^o]M¢J,:?ñ9"63!
1,9ÎÔH? qå>×{glof^XOm·M¹Â. "J¿>¹¦êÐÌ>DM´~Ö<
×{glof>ÏÞÝMßD3Ò³ °>±Ú×{glof>ìÆ=«.3 !ø=#*7'
>tØyMË.3( y%@pëóêÐ:doQTYaO_iO_:M1K2Kôè¬Ç'7ôª=
´~Ö<×{glof?
Ô?Nm_i\m'HPm`doQTWO`MÁ«/J¥
M
R5 #%>@-,GB<3!G>@9$2'$M$ "G*F+OPQN):$ 768 G
0"F
M+1G&G(I/J=ED9EH
GKAC "G+;4."G
?L9H$ 768 +OPQN)H3!G>@:5
Ô¡Þãq^u No.30 (2009 June) 19
¬ð;gPeup:car° L-J
.JĖ¡°6)4ÌÕ*I'65Ć
ÒÙ9-J.J:÷iruhLĘØ*
I'69µ)/
(G9öÏ}ć°:æ{Û°L
Iēé6)4ÝGJA/Č§Ôêò5
â^sfVĉ6úL«µ*I'6
5-JG:Ì±L®)4I'6D·
º(J4IWp^_Rs aos[g
Pou]:ēéÌ±Lþ*I÷
iruh:ĘØLø1/ ;Zca
rVrrjs]sEks^gpRr¢²
ĝĔě87ĚÉ¹ÀÈÏLô
ĉ6*I'6"ÝGJ4
HÐ9 Zcar¢²ĝĔNmb; :đ¶Ù8
ôĉ68Hēé°:ëÃĤ2:c
ar"Wp^_Rs5í¸(J/°
ÔµÏLxI'6"½G!681//
C':Ð¯I¡°LÌÕ)4Ô
bc!#&
LA;JR^UVQ` 0*W\`SXP\aT
(-01)Z_aY ,/+1*.2 E%D:3E
-01 E]N^UO[ D@H?:<4Z_aYL
E7MD$:<A9K358>6E7MFE -01
7"DG59ACB7H?I6AC=<4'
êò5:Ì±ÆLó6*I÷i
ruh:ĂĀLø1/Ù9;vč)/Ěá6;Ď#:*8K0car:ªĚ
©±93$÷!Gjs]sÓĒ~=: LÌÕ)ĥ9à*»ý Ì±Æ÷i
ruh L`XOstĘØ)/ ĘØ9µ)/ ; 6°*I; QdpUu:9Zcar¢²ĝĔN
mbĒ~"9 *I/C 9FH?@Î÷5I'J" 9F14Wp^_Rs(
JI'69FH "Ċ'G8$8H
÷ª¨"ĜċÙ9*I'6"çù LÕ/ ¤Ğ!Gß!CGJ/Ð9ªą*>#Í6)4Áiruh;Wp^_Rs:B6;?6M7°,
+ " )4C4Wp^_Rs°"Đø*IÐėLÀ)4
HÜ9 Ì±L6GI
'6"ÂIyÖ:÷iruh5IÍ"&GJI-'5¿¬9 LÔêò9Ĉõ)
Ô#4Iêòz: Ì±:ÆLăB/-:ëÃêò:âě9F14Ô#4IÑ´5: Ì±;#$×8I'6A/ 87"Mêò5;ĥw9à)/F9êòĉ9Ê>4Ä
9ª Ì±"I'6"½G!681/':F8¾äęÿó:ġ³;Ôêò5È
ó*I Ì±Æ÷iruh: "14C4GJID:5HÔÏ¡Þã9
%I÷
iruh:ĕû±LåÙ9à*ëÃ5I
+* )$
!&'"%#(
(G9¿ČgpRq\Os:ĠÅÇďLñ9üÚ*'65»/8÷iruh`XOsË9ì
"IĄ¥î:ù9µ)/*8K0vā: :ï¼9FJ<SplTY;|:£ó9
¸*I'6"ó8;+5I6ïn_pnaTY87|:£ó9í¸)/Ą¥:Ę
Ø9µ)/ĢĦ¦vģğ/'69'JG:è8gpRq\OsĄ¥;»ýÏ5H
mdv £THX No.30 (2009 June) 20
3%][-´¶#96-!:7/4),«_OUXN+,95.*'%%4!:7.
«_½< ¾][ +#¿+ml"% -!:7 .«_}<¤"%¨
PVGV >MSBXTPS.Z-68«_ºugw#9+ .e-Y©"%
¨{7:%08*,
«_ .s/ADVJV·^.¹q=L?Vp*9r
k+aup*9rk*t
,9!+57+,'%¾oÀiZ¿¬/OUXN³².
¯7¼y-*9#,;&oÀiZ.?TVF.*n'% QKAEKS>V<PVG
V·^+")( /ADVJV·^=L?Vp./z«_}*9Yaup-,9+
12«_*9+,}µ<#9ADVJV¥ª¦+±4),«_OUXN
+,8{9!+<¡")9][}<")»-~b"%j¢ca°¸¦Wf|h®d
«_OUXN<§\#9
3$@RCKEIXG}`«_OUXN ¾oÀx[¿<§\#9
/
J >~A S[X^[P\VVZ]u&Drku+2s=+agu+"u=+}
)/il@?(tof`%ypIdn.794qu/e:s0u_M$=
SPM_PTY uF1t
ilaxl@?(})/i`%ypsc68*Etzmp`!Gu=
)}-j{ > NTWVU =;5KNTWVUJSP HLuF1t
ilaSPMQRO }B<il79qv`Ch|p
c{32u NTWVU =;5t'wpsc*Eq`I,t.79u=#;e:qbmlaJ
äªíò}o No.30 (2009 June) 21
yrhkr|eaANwnãĝJ?] ĪI1\/;Nv~uĂĔNhjqãĝN
Õğ¤O/gzirmJû=H2]eq|ÏĘJLEH4\/ZEH ) NÕÛÙIX
©¡aJ]ûË/STÜĆ»I1]0;^6.gzirkpnMZ\ßçéMÕĊ<^];
JI/hjqãĝNÕğ¤Ot{JL]6/AN ) 6ø I1]BWĀv~r=Hc
sf¡JL]BW/ä¾ÞI1] "" Oµ2Ćaè?]0?L`DÉv~uO.g
zirkpnØ»ÍĆv~uJ=HÑÿ?]0¬Ħ/Éº¸INĆ¥¨áOø M
Xě=/ÎWHĠÀM.gzirkpnØ»Í6ÿI1]0
.gzirkpnO}xoĞùJ=HÆXÖå<^H2]6/;^UINÍ×O % ÌĄ
LKN×aâJ?]XN6SJbKI1\/âéMĭ½´I1]O@NĆ×6å2[^];
JOĨ³M±L5EB0ġèM¾=B % O©MöĝaİF=5¿B@/;^6Õ
Ċ<^]İÒĥNºI/¹Ê5[ë[^H2]Ćv~uĮ%-(*"+ "$'!$#% ,(+$!"/
Ĳ²įNıÒĥMê¶?]Æ¨NĆµ´¦aä>]Z3MČċ<^H2]BW/Ĩ³M½´
ă8.gzirkpnØ»aÍ?];J6ÿJLEB0Év~uOäúþ÷QNĜå6ÿ
I1\/¬Ħ Ĳ²Mð=BZ3M/äúþ÷M4:].gzirkpnNĭ½´ÍMæI
WH¾=B 0
ĖNČċ×Iå2]c|h|¤ĝO/XD_bgzirmMģ[^]XNIOL20;^a
ĊĤ»Đ¤I1]sr~wl|¤J?^P/2`Y] #"! ĆĄù6Ďä?]0¬ĦÉČċ×M
¤G2Hġè<^B O/A^ĂĔO© " NûËSTÜĆI1\/;;M '& NĊĤ
aÝ¯?];JI¨7LĆ¥¨að? #"! ĆĄùJ=HÑÿ?]0 OĖN %
JÐ/İÒĥNĊĤ»Đ¤NĀħMZ\Æ¨NĆµ´¦aä>]BW/¹ÊĄùMÓRÎW
HìÅĢNĊĤÝ¯IúþNĆÌĄ6ÿI1];JXð<^B Ĳ0
ôý[6Æĕîó?];JM¾=BÇÑ°©Ćv~uNďâéLČċ×MZ\/ġèÿL
Ćv~uNñĪOīēéM¥¨=/ä7H2]äÞčÂNIĒ;]ñdwraĉ?]Ã
ĈĆv~uüNġèM¾=B0ġè=BĆv~uüO/úþ£ĬÙMÚ?]C:I/Ä
MúþāaėĚ=Hä7H2]úþMđą?];J6ÿI1\/?9MIXäÞªĩ¢íòMĜå
ÿL¬å»a¿EH2]0;NZ3M/ªa¤ïJ=BĆv~u©aõ®MČċġè?];
JI/äÞªĩ¢íò6ÔMęV;J6¨2MÈ·<^];J5[/¸X§ñ§ÐLÑÿa¿FĆ
v~uNġèaĠ¼ćEH28«I1]0
Áà
1. Akwasi Minta et al., J. Biol. Chem., 264, 8171-8178 (1989).
2. Tetsuo Miura et al., J. Am. Chem. Soc., 125, 8666-8671 (2003).
3. Kumi Tanaka et al., J. Am. Chem. Soc., 123, 2530-2536 (2001).
4. Naoki Umezawa et al., Angew. Chem. Int. Ed., 38, 2899-2901 (1999).
5. Ken-ichi Setsukinai et al., J. Biol. Chem., 278, 3170-3175 (2003).
6. Tasuku Ueno et al. J. Am. Chem. Soc., 128, 10640-10641 (2006).
7. Suguru Kenmoku et al., J. Am. Chem. Soc., 129, 7313-7318 (2007).
8. Yuuta Fujikawa et al., J. Am. Chem. Soc., 130, 14533-14543 (2008).
No.30 (2009 June) 22
9. Yasuteru Urano et al., J. Am. Chem. Soc., 127, 4888-4894 (2005).
10. Mako Kamiya et al., J. Am. Chem. Soc., 129, 3918-3929 (2007).
11. Tomonori Kobayashi et al., J. Am. Chem. Soc.,129, 6696-6697 (2007).
12. , , 381, 44-50 (2002).
13. , 37, 370-374 (2005).
14. , 57, 699-706 (2006).
15. , 61(9), 23-27 (2006).
¾ulÊÐJ8N No.30 (2009 June) 23
UÀ jĂ (ă
T~~û46:D¾uÎ 46:D¾uÎ Oîm{ëÏ 4 ¸ÊÐ
[email protected]
¾ulÊÐJ8Nì¢ÙX2N=N
yÒ[,ÿ&(íâ!
»xÍT~~û46:D¾uÎ¸_¬ QØÝcHLöl
õÖĂ/=N7ăÓÞÄ)46:DùÄ,ç
(!¨Æá
¿qÞô\/FHGNºõÖåb-EN5L146:D
¿,ç
Û!WvÃØÝCN.Nªe46:DÃi¤ÈégY
zĀØÝ,`¿ä¹×46:Dì¢,**ÙXÿ!
Integrated Barcode Chips for Rapid, Multiplexed Analysis of Protein in Microliter Quantities of Blood
R. Fan, O. Vermesh, A. Srivastava, B. K. H. Yen, L. Qin, H. Ahmad, G. A. Kwong, C. C. Liu, J. Gould, L.
Hood, J. R. Heath, Nat. Biotechnol., 26, 1373-1378 (2008).
§ì¢C-0K³ñ,¿>-6INB9;]26;āÃØÝCN.Nf
ªe²,ËÑ!ÃÜæSº8L?0ïĂÃCN.Nfăf±ø
~sP
(æSø,µ)Ô¥ÆÃê£ç+*!a
hÑàÃÜhÑàºÁo (Prostate Specific Antigen; PSA) nMÕßÂÜ@;
Ú¯3=<;KAL (Human Chorionic Gonadotropin; hGC) æS·Vó!p
æ´S8L?0ïRÉ¦úd½)°#'*!!&CN.
Nf}¡(r¦}¡ª^,d½)$r«÷è(!
w 1(A)ãÜ'ùÄ IBBC
(Integrated Blood Barcode Chip),Ì
!æ´SÇÆC
N.NfZ|þ}
ü¹x!!8
L?0ï®òþ~
ðæ¼%Åæ¼Zweifach-Fung
k©&(ÇÆCN.Nf,
t"æ¶ffý)q
Þ*C-0K³ñ,`
«f^qµ»C6G No.30 (2009 June) 24
HX¨'"&Ì¥WK&³±@G0GWp+V(Î
'"
@G0GWpu(P+ètzSæ DEAL (DNA-Encoded Antibody Library) ;G3
G:¡%')+Þ"éh 1A ×êDEAL ¡mºP+HzSæ(#
m@G0GWpW ß+Hz¤r(
`Åh 1(B)Z¼È¯Ã&_
Ì¢4E>BK PSA % 11 º4.90.EW C?B¤rÁ12 º@G0
GWp
äx)WãFV(
W'"h 1(C)Ð 22 MZ¼È¯
ÃéP01-P11ê%L¯ÃéB01-B11êÌ¢4E>BK PSA W C?B+¤rÁ
L¯Ã4E>B!, PSA ¶Ó)uZ¼È¯Ã4E>B
èW (
W'"& IBBC %(¤rÁ6E<1ÖV¡
¬)( ELISA (Enzyme-Linked ImmunoSorbent Assay)¡bV>D=-.B%
z
~&)V¡¬
·)""¤rÎÌ¢4E>Bß
μL /G7Gäxßv±¿Q¯Ñ¡¬
})(jd
'"
Stimulation of Tumor Growth and Angiogenesis by Low Concentrations of RGD-Mimetic Integrin
Inhibitor
A. R. Reynolds, I. R. Hart, A. R. Watson, J. C. Welti, R. G. Silva, S. D. Robinson, G. D. Violante, M.
Gourlaouen, M. Salih, M. C. Jones, D. T. Jones, G. Saunders, V. Kostourou, F. P. Sierra, J. C. Norman, G. C.
Tucker, K. M. H. Dilke, Nat. Med., 15, 392-400 (2009).
mi{¯Ì½T²ÀÄlgp(Vascular endothelial growth factor; VEGF) Ý\°©
'¯Ì½«+Ôw¯Ø¸+RÜ""ÀÄ´gp(.E8
2AE$Ì½«£áJ'.E82AEâs[ (Cliengitide) ¯®Ë
ÉyÒç
Í*)(K'"
&Cliengitide ÆÇuHr
®]+~"
N¯ÊoÁ
~&)",Õè§z¯
F
F
F
®]
¶Ó)( Cliengtide%OWpâs[ S36578 O§zÛ¯à+
RÜ"+ÏV"&)&áJ(6E<1ÖVEGF
C5>6G6E<1Ö (VEGFR2)v3 .E82AE(v3)6E<1ÖA4.1AE2+Ôw
( RabA4 +"h 2(A)O§z (2 nM) S36578 Uª%( VEGFR2 % v3
ÀÄeÚ
§Â]+¶Ó"VEGF %'ÀÄ+Y¦(Y¦$Ù
VEGFR2 ÀÄeÚ°©ß
cI"&)+O§z S36578 Uª(
UªkaÙncI(
¶Ó)"O§z S36578
«Ì½¾J(|å+
¶Ó"éh 2Bê"À
Ä+ VEGF Y¦(Ì½
«
¶Ó)"VEGF Y¦
ÀÄ+è§z(50 μM) S36578 Uª(Ì½« VEGF
Y¦ÀÄb¹z"(
`ÅHO§z
ÕxâæR>V No.30 (2009 June) 25
S36578 lÔ+VEGF sÍïö(*ËÞāé³ÕĂ.,+n
*$¶
N@QK2;/ÖÝ¦ĜY+w¾/ãċ$Ýñô S36578 /Å
ÎlÔ-1 mg/mL ^XÝ¦Ĝ/©r_Î(0.1 mg/mL)lÔ
~9TASVQÇĐ¦ĜeĔ,+ãċ,$,)ò¾
U
U
U
(*_ÎĠu(*(1) Rab4A Ë¢x Ī@V>ºđī(2) RabA4 (+ VEGFR2
(! v3 ïöĒ#Îó ( 2 A)(3) VEGF (+³ÕāéÄêeĔ ( 2 B)(4) Ý
¦ĜČ ( 2 C),+WēM4C<L/®$ġÈÜþÐ
ù×«¯(*ÝĒþÐÎÆè_ÎÝ£x+¥¡,$
½ßÝĒþÐÎ/x+¨ă+¼²ć+
'
,$0
High-Content Single-Cell Drug Screening with Phosphospecific Flow Cytometry
P. O. Krutzik, J. M. Crane, M. R. Clutter, G. P. Nolan, Nat. Chem. Biol., 4, 132-142 (2008).
ÑÚÛïöÑ>TE6ďPTĘxėíĪ5BV=īË¢Ù\Ĕ+
,$&,)ėíĠuÚÛÈÜþ+¹$
ÑÑėí(!ÑïöĢ%aÖ+ĠuvaÖ/Ė+XĤ
·Ö$,)ėíĠu;6PVCT7ìĝÞ'Â&ęć
105 gz"hĄþÐëąėíïög`.+ĉdĂ.,¶ðß°g
þÐøĊĨ$.,$WÆ;6PVCT7[ëąėí(+Ġ÷ĉdĂ.
,$ëąėíĉdúò¾),þÐ'Õ`k·w.
*$0$ëąėí(+ĉdüµĞĎěć*$&(*w
ÒßþÐ¬î/àßïö/qÖD1;QVI?A;6PVCT7ìo¸,
$»č±GSV:1AMAPVĪFCMīÑ¢/qÖ+µåïö
>TE6ďË¢/ĉdD1;QVI?AïöJV;;6PVCT7ì/Ó$
3(A)§Ø/äÃ¡ĪPhosphoflowī*$p&þu(*àß>TE6ď
Ë¢/\Ĕ$ÆþuhĄx~Ð/lÔàß>TE6ď ÿifĦª`
ĪĆ°>TE6ď>V8?A~fĦ+ÿiÿiÊĜ/+¶ 15 å$|
÷ī/aÖ¿û$¶ FCM Àm/Ă$tª`Î¿ûµ
ĞEOMV>V/¶ĕx*23;>THS?Aò¾Ĥĩáğ/$
ĪCytometry A, 55, 61-70ī
$235 gx~Ð Phosphoflow (+ħïök Stat1Stat5p38 Ë¢Ġ÷ÀĈ
/Ă$ 3(B)Z 12 gF?AþÐò¾/ä$c
HHT
p38 ·wĠuStriatin E Stat1Stat5p38 j·wĠu+n
*$
(WÌ),+hĄþÐ¤Ěĩ;QVI?Axć¨ă
*$
)ýõ)tãç FCBĪFluorescent Cell BarcodeīÉ ( 3(C), Nat. Methods, 3,
361-368) Phosphoflow /Ā~ Phosphoflow/FCB É/p]ħïö Ö$Ñ
ñô
)yWp]ħïö/+&ÏģëąaÁĥW´}ïö
U
U
Ñ{`ÞÓ*,)+ÿifĦª`/bÖ+ëą+
WO`8/; No.30 (2009 June) 26
a(x
L%S'Phosphoflow "$]£
¤bNw(FCB }"$]c%¤bNw(%
<X~ag#
&%i[¨¡U=Y 3(D)Phosphoflow/FCB }#kRu
M?Z©«B T Q{V&%¬K Stat1 c% STRPN ¤
bNw(~a$B K Stat1 cdD_¤bNw\^%
&<qCD4+ T K Stat1 cJ¤bNw(>)
w+,./:4901nTh!ªE¦
%wsf#
STRPN ¤bNw(FA867yIM?Z©§@wg#
&
"Phosphoflow/FCB }(C%G]/:3- "
(VM?Z©c%¤bNw(¥Bz(2*.7;501
~a%
vo¢)vj}(1) IC50 H(|
a%(2)mPF(<r~a%!SpM?Z©(C
l}te&
No.30 (2009 June) 27
$ %
"#
! 3 [email protected]
²Ã9Ä¤]%1ioêÚî¦TÂÁ¼»G§Ñ*s·ÎSÑ«¾ ¯¤Â¨Í®Àª³µ©È
·¥lÄ..1^Om1io@ &1ioÂ¾FD¤.k`X/FDÃ<C
4ÃÊÀioÑ¼¾¬ÍÈ·¥/ GTP z#ÚìãÕÄ
/e[¤w~$LÌd:?í
n À 6 7 ° ¶ ¤ ² Ï Ì Ã | ; ¢ Ñ B ª prenyltransferase Ã farnesyltransferase À type-I
geranylgeranyltransferase ÄA®ÒÃRfvÀ¶¾Vg´Ï¾©È·¥lÃioÄ²ÏÌÃ½Ã
vÂËÎ|;¢Ñya¶¾S¶¤·©®ÒÃ 90%Â-c®´Ï¾©Î K-Ras4B Ã|
;¢ÃÉÑNfÂ2·Î prenyltransferase dual 2Ãe¿·¥
'Ä¤ÚìãÕÃQ-Âhg¶»H»ÁÙÕèîàìÖUe¤RNA Â¬±ÎèæáÕêÔ
ÛÞÃH»Á#?y¤/ÂËÎØÖßé
Ã_S8ÃIÃ½Ñ³x¶È·¥
A New Screening Assay for Allosteric Inhibitors of cSrc
J. R. Simard, S. Klüter, C. Grütter, M. Getlik, M. Rabiller, H. B. Rode, D. Rauh, Nat. Chem. Biol., 5,
394-397 (2009)
kinase 2Ä i) ATP Àr#¿¯¤£°0´Ï» kinase domain Ã hinge region Âz#·Î type I À¤
ii) hinge region ¬ËÅ¨ÈÍ0´Ï¾©Á© allosteric site Âz#¶¾ kinase ÑW>)Â·Î type II À
III Â¡¿¯È·¥©¸ÏÃ+#Ê¤.¯Á library ÌÃ#ZÃÂÄâÓÙéîåÜÝÙÕèîàì
ÖïHTSð®Nf¿Ä¨ÍÈ·®¤#ZÃRfÚìãÕÂ3¶¾Ãz#Ã¡ÂÄ HTS Ä¶¾©
È¹Ò¥²²¿x·ÎGÄ¤kinase 2Ã type Ñ¡¿¯ÎH¶©ÙÕèîàìÖÑe¶»À©ª
G¿·¥
kinase ÃW>)Ä(Ã"qÂj·ËªÂ active loop ®©¾ type II/III ®z#·Î allosteric site Ñ
K¶¤ATP ®z#¿¯Îç×ÜÝÑÍ·ÃÂ3¶¾ï( 1 a b"ð¤W>)¿Ä active loop ® ATP
Ãz#Ñ2¿¯ÎËªÁ{Ñ!ÎJÐÍÂ¤allosteric site ÑE·ÎpÑ]É¶È·ï( 1 a 5ð¥
s}ÌÄ²Ã active loop ÃQ-Âh
g¶¤kinase Ã½¿¨Î cSrc Â\,=
u)ÃåëîäÃ acrylodan Ñ4
¶» cSrc Ñ_©¾¤W>)Ã cSrc Âz#
·Î type I ÀW>)Âz#·Î type
II/III Ñ¿¯ÎÑÉÈ¶»¥2
ÃÁ©M¿Ã aclyrodan-cSrc Ä½
ÃeP.Ñj¶È·ï( 1 c 475 À 505
nmð¥º²Â¤type II/III Ã2Ñ_
´¹ÎÀ½ÃeP.®ÆÇt¶°Y
%#'
& !" % $ ') % '(
No.30 (2009 June) 28
=ºÄ®µÉÈ<ºÄ«type I É ;Øq¹½À1)ÅÊ«475 nm Ét`6ÉÎ¯³ºµj=º«
505 nm Ét`6³ 510 nm ÌßèãºÏ¼¬·ÉÒ¯È active loop Éa4#È®êðóéÉo
3±ÒËIÈ4#³p»Õ·ÆØqºÄ«type I Æ type II/III Ø$³Å´Õ·ÆØwºÄ®Ï¼¬
© ÏÀ«yÓ³tºÀ-É assay {ØqºÄ«p38 kinase È nM Æ¨® affinitiy ØMÃ type III
;É scaffold ÆºÄ(ÔÖÓÖÄ®Õ pyrazolourea Ø^Æ¼Õ 4 ÃÉ#)mØ)Jº«cSrc È<ºÄ
ÃÉ#)mÉ IC50 ³| 30~65 M ÉhIØwº«type II/II Æ*» binder ÆºÄc¼Õ·ÆØºÄ
®Ï¼¬¹ÓÈ«¾ÖÓ#)mØq®Ä cSrc ÆÉYØJº«typIII ;Æ*»)b@ÅÕ·ÆÑ
wºÄ±Ô«-tºÀáÜíóåñÝ³Tº® tool ÆºÄ["ÅÕ·ÆØwºÄ®Ï¼¬
Synthesis of Activated Pyrimidine Ribonucleotides in Prebiotically Plausible Conditions
M. W. Powner, B. Gerland, J. D. Sutherland, Nature, 459, 239-242 (2009)
íëæÜïÛâä³)ºÄ RNA ³BJ¹ÖÕ·ÆÊ:
¹ÖÄ´ÏºÀ³«¾ÖØaJ¼ÕÀÐÉhI#¹ÖÀçíì
àñíëæÜïÛâä 1 ÉBJÊWÓ²È¹ÖÄ®Ï½ÙÅº
À¬·ÉRÊ«·ÖÏÅÈ°ÓÖÄ´À~ô£õÆ*»
8§Øq®Ä«ÉØ~rºÄhI#¹Ö
ÀçíìàñæÜïâäØvµ«¨"nÇîóãôõØtº
ÀÆ®¯ÑÉÅ¼¬
1 ÊçíìàñæÜïÛßä 2 Éíñ#ÈÒÂÄ«2 Êçíì
àñ 3 Æíëóá 4 É)ÈÒÂÄp»Õ·ÆÈ®ÊÇ®
Æ°ÓÖÄ®ÀÉÊ«3 Æ 4 ³)J¹ÖÕÆ®¯:ÈÒÂ
ÄPM¹ÖÄ´ÀÀÐÅ¼ô£É~õ¬º²ºÇ³Ó«2 Ø
)J¼ÕÈÀÔ«3 É N-1 É¤[¢8<É¤>/#±
ÒËfkiÅ&G³)ÒÔÑ!f³7ÏÖÕÆ®
ÂÀ·Æ³«·É~É,¦ÆÇÂÄ®ÏºÀ¬¾·Å«y
ÓÊ.ÉÅw¼TÀÇ~ØN+ºÄ«¾Ö¿ÖÉÆÇÕ 11 ²Ó 12«13 ÈÃ®ÄÉ&G\
É_Ø®«¾ÖÓÉØ~ÄhI#¹ÖÀíëæÜïïÛâäØ)J¼Õ·ÆÈJ
ºÏºÀ¬8 Æ
10 ÈÒÕ 11 É)JÊ«A20ÅÉ)³uÇKgÅÂÀÉÈ<ºÄ«I\
ÅEÕÀÐÈ
lcíñ2Øq®Õ·ÆÅ>80%Å 11 ØEÕ·ÆÈJ
ºÏºÀ¬ÏÀ«·É&GÅ 8 ØÈ¼Õ·
ÆÅ 3 É%SÆÇÕ urea 6 ³EÓÖÕ·ÆØºÄ®Ï¼¬dÈ«9 Æ 11 ²ÓÊ 4 ÃÉsI³BJ¹Ö
Õ³«lyxose«xylose Ê¤?È'n³µ«¹ÓÈíñ29/ÅXQºÄ±µÆz³oºÀ
BÌÆ4O¹ÖÄºÏ® arabinose 12 Æ ribose ³ major ÅpJ¹ÖÏ¼³«ribose Ê·ÉÆÉíñ
#³p»Ç®ÀÐÈ«]ÆºÄÆÇÕ 12 ³pJmÆÇÔÏ¼¬¾ÉD«12 ²ÓËlcíñ
2Øq®Ä«&GÉ pH ÉVØL°Õ·ÆÅ anhyrdousnucleoside13 Ø¨'nÅEÕ·Æ³Å´«13 ²Ó
1 ÌÉíñ#È±®ÄÊ 5’É 1 }ÚîÞóîÈuÈp»ÕÉÅÊÇµ«3’É 2 }ÚîÞóîØ
ºÄp»Õ·ÆØYa²Ó 5’Éf0³ 3ªÆeºÄxuÇ"]ÈÒÕÑÉÆº«&GÉ
kiÅÑ¾É"]³C¥ºÄ®ÕÆÍÄ®Ï¼¬¾ºÄ«3’Éíñ0ÉØ~rºÄ«hI#¹
ÖÀçíìàñæÜïÛâäÉ)JØJºÏºÀ¬:¡È¸ÈÇÓÖÀU³5®ÉÅÊòòòÆH®Ï¼³«
íñ2ÊZDÉæÜïÛâäBJÈ×ÖÕÁ¶ÅÇµ«ÆÇÕ&GØF¼ÕmÆºÄhºÄ®
No.30 (2009 June) 29
zyRNiPNS(¤*&¤¡} £{£y+t¡}7§k
RNA
;¥8}¡}g?z
Sialylated Multivalent Antigens Engage CD22 in trans and Inhibit B Cell Activation
A. H. Courtney, E. B. Puffer, J. K. Pontrello, Z.-Q. Yang, L. L. Kiessling, Proc. Natl. Acad. Sci. USA, 106,
2500-2505 (2009)
x B Y^=¹³®
A
B
»¼BCR; B cell antigen
receptor½ B Y^§I
9¤ BCR ¬«°¸
§Y^~¤4
§
>

acid-binding
y
sialic
Ig-lectin
3 A: polymer B:
Polymer 1-3 2+
[Ca ] (Siglec) ² ¨ µ · »
CD22 BCR ¬
«°¸§q,¤
-V¢¥£yCD22 BCR 0&¡ BCR ¬«°¸I9§<~¤A¢
|z@yCD22 ligand §>X®º±ªi CD22 !Y^avUQ{¤ cis
interaction BCR 0&|yB Y^I9P¤'"¥|z
y¥/MT CD22
B Y^avX®º±ªiUQ{¤ trans
interaction y¢P¤¬«°¸3wCBzZ¤g? B Y^
I95¤ CD22 cis trans interaction $Fp§A¢g?z
x % 3 A W¡}yBCR antigen DNP polymer 1yCD22 ligand polymer 2 ¡
antigen ligand §[
¦ polymer 3 polymer §Q| B Y^I9O6{¤[Ca2+]
)c.§`|z% 3 B W¡}yBCR =§> 1 BCR \
Y^avX®º±ªi\
yCD22 B
z¡ 1 §QyCD22 BCR ¢su¥2§
¤ B Y^I9<nb{¤0&j¢I9§lzG 2 BCR
antigen §>| B Y^I9§l||}:k£\F§Wz3 |
_#K|yantigen ligand 2 §> 1 2 QoI9§W]~¢¥
yB Y^I9§l||}\F§Wz¥ 2 BCR antigen L| B Y^
I9l¥||}\F/y3 \F antigen ¡ ligand ¥¥eh¥
CD22 BCR 0&§zy¥ polymer ¥¥ antigen ligand §
D|¤{¤]~¢¥y1 2 Q B Y^I9§l¤|}\F¢y3
trans interaction BCR CD22 Q0&§1jyB Y^I9§<¤§W
zy1 3 BCR antigen §> B Y^I9 BCR signaling EHrl
¥zytrans interaction B Y^I9<§dY¤yBCR ·ºm¯¶©º
kinase {¤ Lyn y¢J SykyPLC2 ·ºm¹´¸§f\FyLyn ¢J Syky
PLC2 I9§<¤§A¢z
No.30 (2009 June) 30

R Ý¼Á«³ ¾£Þ
PmWm*, aNE0,jp< W,ujpm 'nß2
[email protected]
¥´3µa!,jpÙÐÜ´Ds´V
Å£³9z§¸¨_#kµPmWm
*,´l;CA´°[rvÐÛÒÉ;³¿ÂxÐÛÒÉt×ÕÏÜÖ´}±Vw
\³xÐÛÒÉ¢Vw¨Â]:°^{¤×ÕÏÜÖ³t¹º=Y´jp³BÄ¯ Á¸¨
¥Ã¸°µxÐÛÒÉt×ÕÏÜÖ´}Y±§¯µ³rv³hf´xÐÛÒÉÅ4e_¦
©dZ8qÅc¯>§«¼´Å×ÕÏÜÖ³T;¨Â=Y¢cÀÃ¯£¸§«¢
2[rvÐÛÒÉ;³¿¯;§«xÐÛÒÉÅ×ÕÏÜÖ³t¹º=Y¢e¦ÃU²
xÐÛÒÉt×ÕÏÜÖÅ5;¨Â¹¢²¦Ã¯¸¨¥´¿²=Y´e/³¿Á¥Ã¸°Li
²´)xÐÛÒÉ´TQ½Vw´G³¨Âjp¢º¥±³.H³xÐÛÒÉ
t×ÕÏÜÖÅ}°£Â«»bS7c¼gÆ³|ÄÃÂ¥±¢K6¦Ã¸¨
Mo°µ[rvÐÛÒÉ;³¿¯xÐÛÒÉÅ;§×ÕÏÜÖ·´t¹¹Å-F§¯
ÂD³®¯$s¦©¯«¬£¸¨
A Synthetic Biology Approach to the Constitution of Membrane Proteins in Semi-Synthetic Minimal
Cells
Y. Kuruma, P. Stano, T. Ueda, P.L. Luisi, Biochim. Biophys. Acta, 1788, 567-574 (2009).
×ÕÏÜÖµª´5]8¡À0rvÅ5;¨ÂNE±§¯Jc~¦Ã¯ Árv´?®U²
Vw¶ÐÛÒÉ;wqÅ×ÕÏÜÖ³?¦©Âjp¢-F¦Ã¯¸¨MD°µ×ÕÏ
ÜÖ³y1&XwÅ?«©Â¥±Åh@§×ÕÏÜ
Öx´T;;Å×ÕÏÜÖ°;¨Â«»³
¡ÀÔÍÓÇÑÌÛÅ;¨ÂxÐÛÒÉ
Å[rvÐÛÒÉ;³¿¯×ÕÏÜÖ³
t¹º¥±Å¹¯¸¨Ý"1Þ¥Ã¸°³¼[
rvÐÛÒÉ;Åc¯xÐÛÒÉt×ÕÏ
ÜÖÅ5;§xÐÛÒÉÅVw¦©Â¥±³;
§«$
µ+#§¸¨¢MD°µÊ×ÎÚÜ
Ø-3-×Û±ÈËØCoA¡À×ÏÔÍÓÇÑÌÛÅ
; ¨ Â x Ð Û Ò É ° Â GPAT
Ýsn-glycerol-3-phosphate acyltransferaseÞ±×ÏÔÍÓ
ÇÑÌÛ±ÈËØCoA¡ÀÔÍÓÇÑÌÛÅ;
¨ Â x Ð Û Ò É ° Â LPAAT Ý lysophosphatidic
acid acyltransferaseÞÅt¹Æ¬×ÕÏÜÖÅ
´×ÕÏÜÖ³t¹¹`%OÅ°(I
No.30 (2009 June) 31
¨®°¡2ftÍâÖÄ¼ÉãÆâÉÝà¬W¢¨·@lV8T©¹ØË
×¾ÎÊâ¼!8¡¹ª¬8¨°¡}¡¹w<tÍâÖÄ¼ßÙÌãÛ¬m±²
ª
r¬«¹ª©¶¸w«Kr¼9¤ßÙÌãÛ8©¹¤³J«5WC3º°
¡°¤ßÙÌãÛ¼I8¡¹sf¬¶¦¨tÍâÖÄ!8!8¤tÍâÖÄßÙ
ÌãÛ¯tÍâÖÄO7[«¹ªµ!8?¬ßÙÌãÛ,'¡¹ª©tÍâÖ
ÄO7«¹ª¦¤R´e%º¨¸1¬u$PnG©¹
Cell-free Synthesis of the Torque-Generating Membrane Proteins, PomA and PomB, of the Na+-driven
Flagellar Motor in Vibrio alginolyticus
H. Terashima, R.A. Yoshizumi, S. Kojima, M. Homma, J. Biochem., 144, 635-642 (2008).
1(`¬k¤~=©®ÉãÆâ
ÉÝà¬}¡¹2ftÍâ
ÖÄ¼ßÙÌãÛ¬m±½©°
¤D~=©®aW¨1§
Kr¼-U¡¹2ftÍâÖÄ¼
SjqÍâÖÄ!8ßÙÌ
ãÛ¬m±²ª¼{±¨°¡Í
ãÇÏÒª¨¹tÍâÖÄ®Vibrio
alginolyticusXEPomAªPomB©¸
4+PomAª2+PomBt
2 1>4, ! PomA # PomB $ ;70?8
"$@Ni-NTA (+=?/*:8' !5
:,.9>$6=23)>-A SynBFTB5=?/
<?WBE1E2B@%&A
©w!¼28¡¹ª©MÜã
Íã¼I8¡¹ªb·º¨°¡~=©®ßÙÌãÛ,'©SjqÍâÖÄ^U¬¶¦¨2
ftÍâÖÄ¼"?¬!8¡¹ª©p¼¬^U¢¹ª¬^U¤tÍâÖÄßÙÌ
ãÛt¬m±°ºpaW¨¹ª¼e%¡¹-ÑãÍeº¨°¡_¬
®PomB¬his6ÍÅ¼
¤´¼^UNi-NTA¿ÃáãËÂÞÛ¼¡ª©ÍÅ¼
¨«
PomAPomBª" Y©Hº¹ªeº¨°¡ä&2åL6«·ßÙÌãÛt¬m±
°º¤tÍâÖÄKr¡¹ª®d|©«¦¤ªª©¸£R¬§¨®4^0B
°º°¡aW¡¹w<tÍâÖÄ¼Kr¡¹2©ßÙÌãÛt¬m±²ª®w!
IxFµKrziAW«Ðãà¬«¹ªo·º°¡°¤~=©®Dg©k¤tÍâ
ÖÄßÙÌãÛ¯m±±)¬´ZO7,'©SjqÍâÖÄ!8¬¶¹tÍâÖ
Ä!8µ!8¤tÍâÖÄ
QaWHy´v»º¨¸¥·´u$P.©
¡
Liposomes-Mediated Delivery of Pro-Apoptotic Therapeutic Membrane Proteins
L. Liguori, B. Marques, A.V. Mendez, R. Rothe, J. L. Lenormand, J. Control. Release, 126, 217-227 (2008).
D~=®SjqÍâÖÄ!8¤tÍâÖÄ¼ßÙÌãÛ¬m±±jq¬ÑßÕßã¨t
ÍâÖÄ¼W¢¹ª©]iN\¬W¡¹ª¼`:¤ch¼->¨°¡_¬®
ÚÒÈâÓß¿tÍâÖÄ7#¿ÙÒãÉË*Rª«¹ª·ÚÒÈâÓß¿)t¬,
'¡¹tÍâÖÄ©¸ÚÒÈâÓß¿)t·ÉÒÄáÛC;¬/¡¹,7ÀÁâÎÝÔ
No.30 (2009 June) 32
µ¹ Voltage-dependent anionic channel»VDACº¯§¸
d?vqoA°§·¨´
X!{X£·
«bqBak´¯¢¸±TcoLSW¦´ª´
¸z¯§¸_M{x]r{p
=a4o:HSW£·«b0VDAC
tBak0zo´¯¢¸±!0{x
u6Tc~´¯¢¸±tVTc
~´¯¢¸±`Y{x0z}xvyr
{p9o³R#'hf-Krxo.*GE
uX£·«bTc´¯¢¸±QY{x0zr
{p
BoQY*X£·«bTc´¯¢¸±mSWF
z68k·²¸§{oSWg°§·¨´
X£·«bTc´¯¢¸±¦´ª´¸wrsl,
j[$U?vPyr{¹3ºp}oX£·«bTc´
¯¢¸±°§·¨´¦´ª´¸{xoSW
¯§¸v_Myu@\{}oSWu1z}
°§·¨´%zX£·«bTc´¯¢¸±Ky|o
¯§¸£·«bq§¶±C2ioZ3
«¸¡D/o¯§¸vMIz}x©µq®
¬¤µ ´·IJzrSWioSWIC
r}¥ "7zoX£·«bTc´¯¢¸±¦´ª´
¸?O^zr{p
zoX£·«bT
c´¯¢¸±Ky|xHCT116p53+/+SWtr
! 41.63+ "
(%&'$ !78#2/-504,
78#78+ 7)*
8
«¸¡9¹46kDaºvy}v[$yto¯
§¸©µvezrxvO^wr{p}o"nrA¯§¸
©µvezrrsU?v+r{p
=a4oHSW£·«b0(0z}X£·«bTc´¯¢¸±vLSWX£·«
b¦´ª´¸{>5z;NqxPz}qo*M&v<)y{p
W-]`Ê½Î No.30 (2009 June) 33
--/&%
913"CG=D@;H=?G:<FH)
$
'AB<=>E
) ([email protected])
JI7285
ÙÜ×³i$¢d=¯v£ÙÜ×d=<l¤B-
'³:¯§ £6¡CMs
®U$¤ÝØÞ«ÙÜ×»Î¸Ì»Ì·a¦,WQ-£(^\;M¡¯ÙÜ×d
=<l£{n¡nb¯zb¤ÒÖÓÕ3¢ÀÄÉ¾ÌÍ¼Ä¼¢¬)°zb¤
R.£ÙÜ×y³qtA¯zb¯7)°zb£+x¤ÁÈÌ¿ËÎÅ
Ï"@Ðy³qtA¯ÛÛÝ&zb¥°¯ºÂ½´Ã¢0¯K\hFª®
ÛÛÝ&zb¤Z£]`/4X°¯U$N£ÙÜ×d=CM£T

#¤ÁÈÌ¿ËÎÅy¢¬¯C9£¡ÙÜ×ve¢¬¯±+H_¤ÛÛÝ&z
b£
P°¢G²¯ÛÛß&zb£P³prÛÛß&zb³X¯T³+
2¢O¯ÙÜ×³d=¯£I¯Bd=;MÏÚ×ßàÞMÐ£c³k

KIMNL!0#64(
WQ¢~¯24-
'¢
ÙÜ×d=<l¤D1\¢X
°¯{n¡;M¯U$4
²°¯ÙÜ×d=M¤ÒÖÔÑ
3¢|[°I(H\¢¤
a£ÃË¾¹ÉU$ª²°®
>!£%¤*L°¯d=
T£oP°¥
Gateway®¡ U$¨¢d=
T³w:<l¤?*|[°
¯d=zb³X
<l¤d=¼µÎÅ£iY
5°¨©8I£ÙÜ
×d=CM³f=¯¢¤j
¡VYm¡ÙÜ×
¹Ì¼¾Ç¶»ÆÌ³k]`g*£
E}³d=J¢u«¯US
*) 6 ,.+ !0)
s+:x{óã÷§ § No.30 (2009 June) 34
³Ô©¸ÒÇ¨iÆ<¦JKÉ²ÒÈ
Ç?¹ÂÎ2ÄÆÀÂ®Ô©

8ÈÛô÷èõÜ×ÉºÍÄ»ÔÿĂýÈ]ª
ÆQÇÉ¨ëÛãÄ*ÊÕÔrmÈêðàíçÿĂý
³t®ÒÕÔø/ú¨`ù©ëÛãÇÉ¨=~Ã
âóÛßïõ×¯½ÍÈì÷ÚÏ¨6g¨ì
òäÛô÷èõÜÝÙæøāþĄùÆÅ³)ËÕÔ©ëÛ
ã×}Ãq»Ô·ÄÃāþĄÈ}
ÝÙæ×S¹¨rmÿĂý×®ÂwmÈÿĂý×
CÔ©']Ç¨Ûô÷èõÜ»Ô
8øÙõÝ
÷æùÎ}Ãq¹¨DÆ
8Sj×«
Ó»¬Q×¯ø/ú¨`ù©·¯¹ÂCÒÕ½w
mÈëÛãÄÙõÝ÷æ×ÿĂý}Ã&¸
¼Ô·ÄÃ¨vuÇEº½T½ÆêðàíçÿĂý×C
Ô·Ä³Ã´Ôø/1¨`ù©ÈqÃt®Ô
}×R&¼Ô·ÄÃ¨ÙõÝ÷æ×nÀ½U
(ÇÌÕÔ·ÄÎ%ÃÔ©¹²¹¨·¯¹½
AÈÿĂýO°QÉO°o³®½Í¨y
<ÇO°×CÔ½ÍÇÉ]ªÆ@5×¯D
³Ô©°Ê¨}q¹½ÿĂýSj×a
d¹¨vuÈÝÙáÈÿĂýSj×-#¹Â|¹
½Ó¨ÿĂý"EÈÈëÛãÄÙõÝ÷æÈ"E
Y×W¹½Ó»Ô·Ä³uÇÖÕÔ©·¯¹½JÈN²Ô\È.ÇÁ®Â>¹ÂÌÔ
Ä¨éñõçô÷îÆĀĀă1}ÈÓ$³,¤È[fÃÔ·Ä³²Ô©
BµV!¹Â®ÔĀĀă1}ÃÔBamHI×Çéñõçô÷îÈ,¤g×°Ôø/ûù©ÿĂý×¢
öÈÅ¿Ò²ÒØÃÎ'ºÄÆÔÈ³éñõçô÷îÃÔø/û¨`ù©¸ÒÇĀĀă1}
ÇÑÓS¸Õ½Ó$Îéñõçô÷îÄÆÔ½Í¨°ÊBamHIÃS¹½ÿĂýSj×¨BamHIS¡
×LÁÈÿĂýÇ&¸¼Ô·Ä³%ÃÔ©}È·¯¹½lF×t»Ô·ÄÃ¨ÿĂýO°
KÉ<p»Ô©¹²¹éñõçô÷îS¡×cHeµ>»ÔÄ¨BamHIS¡×X»ÔÿĂýÉ¨'º
S¡×LÁÒÐÔÿĂýÄ&%ÃÔ·Ä³²Ô©ÁËÓ¨éñõçô÷îS¡Å¯¹È"
EÇÉU(F³90¹Æ®©°ÊÙõÝ÷æÅ¯¹ø/ü¨`ùÏëÛãÅ¯¹Èø/ü¨`ùÎ%
ÃÓ¨<Ç·¯¹½Sj³sI¸Õ,¤ÄÆÔ·Ä³Ô©vuÈêðàíç3ÇÎëÛãÄÙõÝ
÷æÈhRÈ&¼³90»Ô½Í¨vuÈO°È#o×W4»Ô½ÍÇÈÑ¯ÆQ³D
ÇÆÔ©
·¯¹½éñõçô÷îÈ^gÇÁ®ÂÉ²ÒMP¸ÕÂ±Ó¨ éñõçô÷îÈS¡
×X»ÔĀĀĄ1}ÈtÎ²ÒÖÕÂ®Ô©½¾¹¨8sk:ÈUbÇ»Ô;uÆ
7ÎÓ¨ĀĀă1}3È}Ép0_Åt¸ÕÂ®Æ®©UÃ¨ĀĀĄ1}³
ÔS¡ÉHÇåÞÙõ»Ô·Ä³%ÃÓ¨ÇU(F×L½¼ÂëÛãÏÙõÝ÷æÅ¯¹È
×¶·Ä³ZÔø/û¨`ù©Ë½1z¥È}ÃRÈS¡×Ô·ÄÎ%ÃÓ¨ĀĀă1
}ÈÑ¯Ç¨R}È&¼Ç£×GË¼ÔDÎÆ®©
V+^bé×ì No.30 (2009 June) 35
÷÷û$f¼=ÊXª¶©Å»T¼uõøój@¡8Ê<ª²¼¤F`·h¬
ÇöóûüúJíöĊăăċ ýĊĉĆĄýĉĂÿ ĈĂąāăĀïĉĊþĀ ćĀÿĆĄþĂąýĉĂĆąî·Çí"ñîF>J·½õøó¼B
P¸÷÷û$fõøókfÊ
¶L¯É®ÓëÐèÕÚÙà·j@¡8Ê<ª¶Ç
¼Ä ºõøó¼4UÃey¤7z·ÆE]ðò_2·
/_Êi¡Ç¨¸¤·¥Ç¿²j
@¡TÃj@¡ÕÎìä¼tY2¼|O£ÅÂ5G¼CJÊ»o¥@¡Ç¨¸¼GÇ>J·
Ç
öóûüúJ½÷÷û$f¼ÞçëÜêìä9¸B¸¼ÈÊXª¶j
@¡TÊ<ª¶ÇùôúJ·ÍëÒìÛ¸áÏ×¼BPÊ°È±È(0ªàæÍã»ËØà×
Êª¶¢¦¨¸·ùôúWQ»÷÷û$f¼í"ñv!}îÊ§Ç÷÷û$f
¤õøóÊ¬Çc=¸B¬Çc=½ZºÇ·Ç²Á£Å,¼'%A³§È²
c=¼:ÊB¬Ç¨¸¤GÇí"ñlmî¨¼8MK»½õøókfÂ¿ÈÇ²Á
DÆ©È²ËØà×¤¼õøóBP»k¬Ç8Â¨Ç¨¼Ä »õøóBP¼
ËØà×»ª¶½B¸kÊnÆ¬1x8¸ºÇ¨¼S;·ÍëÒìÛBP¸áÏ×BP
¤k©ÈÇ¸¨¼õøóBP»½Â½Ã÷÷û$f¼¤*#ªº¨¼²ÁÍëÒìÛ
¸áÏ×¼kWQ½1x8£ÅrªEiWQ¼a¤¨Ç¨ ª²1x8£ÅEiWQ¾
¼C[º8¤öóûüúJ¼E)¼R6·Æ¨È»ÄÆÓëÐèÕÚÙà·j@¡TÊ
<¬Ç¨¸¤GÇÄ »ºµ²
-»¨ ª²8ÊI~ª¶ÀÇ¸ùôúdWQÊX²&»½õøóâçåæìÖ¼?´À»Ä
ÆõøóBP¼1N¤¨Æõøók8¼RZ9¤¬Çº¹¼
¤V«²¿²ùôúde
yWQ»½ùôú¼ÚëàéìÛõøóÂ*#¬Ç²ÁöóûüúWQ·)swÊ3@¬Ç¸Úëà
éìÛõøóYG¼ÑêÝ¤3<©ÈÇ
Âµ²p¼
»ª¶½\Hgq¼õøóâçåæì
Ö¼.·ÇËßÌÔÑçë¤gwYG¼ùôúXõøóâçåæìÖÊTÄ¦.¬Ç¨¸Ê{
a%2koïÛó No.30 (2009 June) 36
§¹³¢P
>À©Ï±½¼þăü9YÁ&ÂUÑÇ¸£Æ¸¢ßñåïóàþăüÀ4³»Â¢ê
ÝîþăüÁr¼¦Ï Ă¼Ąýąb\Ñ`´Ï±½¼¢ßñåïóàþăüÁ;ÑE´Ï
±½«Q¸£ĄýąÁßñåïóàÀc§»§ÏåìÙèáþăüÂ¢/{}¼"B²Ð¸ÊÁ¼¦Ï¸É
êÝî²Ð»§Ï«¢ĀüĆćą«uG©ÏÁÂĄýą¼-8²Ð¸þăüI[¼¦Ï¸ÉêÝîÂ²Ð»
§¿§£±¨³¸þăüÁÁ7Ñc³»=¿þăü1ÁÇÑ´Ï±½ÀÌÎ¢ĀüĆćąVÁ
_Â/¬®$³¸£
Mtb\ÃK$gÀ
>«ÈJ³§uG©
>À¢Ąýąqb\ÑiFc§Ï¸ÉÁ60Ñ©
Ï±½¼¢¤WµÏ¹¯¥¼þăüuG©Ñ~¨ĀüĆćąVÑf´Ï±½«Q¸£rxÑ©»øúÅ
¾ÁwÑ~¨¹¯¼û÷ôm:ÁuG©_ÑB³»ªÎ¢PCVÁ§|d:ÊyA´Ï½¢<QÁ
uG©KVÑw¬G©Ï¿?zÑN´Ï½y©»§Ï£
±±Æ¼ÁÑÆ½É»¢3ÀþăüuG©Ñ~¨¸ÉÁåðàÖîÑÀs´Ï£¤WµÏ¹¯¥Á
åðàÖî¿Á¼RC½³»Âj§«¢ÁZÀe@´Ï=«¦Ï£¼ÊÄ¸½ªÎ¢ĂĂĆ
rÁI«ãíñáðóéÀ¿Ï½¢Óñ×óàËæÔÛ¾¨³ÁìÓÕóØëñ«±Ï¸É¢±Ð
Ñ'´Ï«=¼¦Ï£Æ¸¢ìÓÕóØëñX:Ñ°´¸Î¢Ąÿā¿¾ÁìÓÕóØëñ
Ñ
º¸Î´Ï½¢èÙçÞÝòìÓÕóØëñ«-©»hgb\Á!««º»³Æ¨£ìÓÕóØëñ
_Ñ.5^]À³»Ê¢ìÓÕóØëñÁTl?Ñ´ÏÄ¬¼¦Ï£ åìÓçÁ åìÓçÁ*PS½³»¢ú¡OpÀH,*m:Á½ĂĂĆ)rÁ½ÑÄ¢²
ÍÀIÑDÒ¼ù¡ÀßñåïóàÃÁâÓäíÜÓÚóØëñÑ¯Ïõ(úö£Óñ×ó
à½æÔÛÀ4³»¶Ð·ÐåìÓçÑ³¢ĂĂĆ)rÀÌÏI¾¨³«¢¨Æ®v"´ÏÌ¨À
´Ï£HI[Á#LìÓÕóØëñÑ~¨+"ÀÂ¢IÑHnc@³¢¶Ð·Ð«ÔðÙ
àóÔ³¿§Ì¨Àe@´Ï£ /(1 No.30 (2009 June) 37
;9>?=
42 4! <:=$
52 <:=$5! # 47A83"
62 ;9>?=
7μ@ 73μ@!&0+)0*$*.0',%1-"
μ'
GAK<CF7EW+(*'μ'X
μ'
?JCIK@ W)%μ'X
μ'
0+'/-A>B4
μ'
#-W(X
μ'
%
W(X
μ'
+'/-W*1*"*X
μ'
VT
μ'
NU
μ'
2
M
:78H
2
S
2
S
Y :78H
2
M"+
2
M
:78H
! !)%* A>B4W$,($).!-X
0.5μ'
&%!-$ A>B4WX
0.5μ'
RQW7J:K@X
1μ'
RQWD8=X
1μ'
5B6;9GJW(3OL X 0.2μ'
W(X
1μ'
%/W$,($).!-X
0.2μ'
+)WX
0.2μ'
'&%!-$(&''&*) ('WX
0.2μ'
VTP
5.2μ'
NU
10μ'
1. Kotera, I. et al., J. Biotechnol. (2008)137:1-7
17:uM M No.30 (2009 June) 38
2nd Switzerland-Japan Biomolecular Chemistry Symposium SJBCS2009
http://www.cbl.rcast.u-tokyo.ac.jp/FBC/SJBCS2009
*;8"?7:tu%)~xih17:
(2009 9 ' 11 %(F)12 %()
*Lpvm}soqAn +
http://www.cbl.rcast.u-tokyo.ac.jp/FBC/SJBCS2009MAP.html
twq
1. Mechanisms and Engineering of Biomolecules
2. Biological Probes and Therapeutic Methods
3. Natural Product Synthesis and Engineering
4. Biological Sensors and Systems
^C0=#92
sjsStefan Matile, Howard Riezman (U. Geneva), Hilal Lashuel, Karl Gademann, Kai Johnsson, Bart
Deplancke, Christian Heinis, Horst Vogel, Ursula Röthlisberger (EPFL), Jean-Louis Reymond (U. Bern),
Don Hilvert, Dario Neri, Karl-Hein Altmann (ETH), Nathan Ludtke (U. Zurich), Helma Wennemers, Marc
Creus (U. Basel)
%)Toshihiro Ihara (Kumamoto U.), Shinsuke Sando (Kyushu U.), Takashi Morii (Kyoto U.), Junko
Ohkanda, Koichi Fukase, Tomoaki Matsuura (Osaka U.), Teruhiko Matsubara (Keio U.), Takeaki Ozawa,
Hiroshi Murakami (U. Tokyo), Takayuki Doi (Tohoku U.), Takeharu Nagai (Hokkaido U.), Mikiko Sodeoka
(RIKEN)
sjsN%)_RP[N1z{|jkrNnl|jkrK\&;`7:g
HW[Pf-G`7:=S_WN~xih7:g<Xf]]d_
`K3_ZP[D
BXfT]g64]W[PbXO17:gyXfI`.g/cN
_EA^$!g
Qf_^Y[PbXO>3@]W[su3@dJW[RebXO5,afY[UV
PO
L )TW«®x x No.30 (2009 June) 39
¦ ®O cN m 3 m | ³8 < 7 7 ¯p °
¦ ®O c s ³
_jI¦®Oc
{Oc.=^zA4 KMY±¤®]¯, 2.5 cm
Q°zvROc^¯o&Oc^\°2+2#$¯o[°ze8>4fl
zt(¨®ª¯Microsoft wordz PDF £ªH
;w°fNmn~{
k¯e 8s Xm °³
¯_°3m 10,000 z07 11,000 ¯)L°3m 2,000 z07 3,000 3mzPq6Cf¡«Ui~{
¡«³http://www.cbl.rcast.u-tokyo.ac.jp/FBC/SJBCS2009_registration
" N m ³
}277y8562x aS@-@a²y±y²x L
B 301
?')')r5u%1V)TWVx x D>x F/
tg³04-7136-5402x FAX³04-7136-3601x
E-mail³[email protected]
hZ¥®§¤®³http://www.cbl.rcast.u-tokyo.ac.jp/FBC/SJBCS2009x
g³`x d9¯c°zD>F/¯*°x
©¬®¢®³b:zE$x AzGJFz!
o3#@z~ç×ë¦ ¦ No.30 (2009 June) 40
% !"(*
https://apply.reedexpo.co.jp/IPBI/jp/form_con/step1.phtml?ex_code=TOKU
^`ï2009 I 7 a 2 ^¦ 10:30ð12:00
9UïfàØÒÓÌÚ
êq ï https://apply.reedexpo.co.jp/IPBI/jp/form_con/step1.phtml?ex_code=TOKU
!"(*-¦ ¥±o&u»°Éku¾# ðÛÝÞÌÏz~°W±ce&uð
Jï0-D;@ ìz~VLíêG@z~|\Z¦ ¤9 6
¦ ¦ ©°É¾ÌãëÕéÒ*À°Éku½1²¶ÛÝÞÌÏz~ª
f$;@;@ ¦ &@z~| :|sR@J t:|@¦ \Z¦ ; S_
¦ ¦ ©^dv ÔëÚ ÙËØÔåÎéÕÜÊæéÒ¦ ðo&upg
Á¾YTðª
f=?&|;@¦ o3&|@z~U Uê\Z¦ E <
*& '+ %#$) ,*& '
http://seitai.csj.jp/div_sympo/
ï^d#@oh#@§^d#@ÞÌÏÙÑÝèÕë
ï^d#@§#@G@ÞÌÏ§F;@ GîCOE ce?ÔÖÙâ#@\z~Xn
bï9 a 13 ^ì^íì13 `íð15 ^ìmí
9ïF;@&ÐäéßÖêxIQ8ì«812-8582 {EHf%¤ 3-1-1í¬{EH57
¤;s Oi 5 )! q ï 8 a 3 ^
v q ï6 a 22 ^ìaí
} (} ï7 a 21 ^ìmí
)! w ì í ï8 a 3 ^ìaí
oh#@ÔéáÕÍâï
vMKï,¢ì15 v§5 r§2 9í¯ÄÀáÖ×ëv
*,¢v¿(»´¹ 1 z~B 1 Âº¨´§q¿ 2 Âº.¨³¾9/¿§v¡È¸²§
2 y¾[2¿A>4¾]½ÄÆ¨
lïoh*ÀÞÌÏÙÑÝèÕë¾4½¼·¹ 1 I
°´§+
` 40 j
¾4°C¨P"q¿vq`nºw¨
ÞÌÏÙÑÝèÕëÔéáÕÍâï
vMKï£l¯ÄÀáÖ×ëv
*9 a 13 ^ï'N 1 `ÄÅlì£l¾Ã§¾,¢v¿Ç¼®í
)!w¿»¼·¹¯ÅÂµ¾º§ÔéáÕÍâ½)!.ºµ¨
4;?nhpR R No.30 (2009 June) 41
HR KsB 5,000 S4 3,000 SQKsB 7,000 S4 5,000 S8 . 3 -NS
=\ 2,000 jmg
'Fs9 . 14 -q.rTH5 6,000 q%Y\7J]rT
7J,1seokfcl[_SWEB dbiqhttp://seitai.csj.jp/div_sympo/rV`9D7JS>>]
+S:L]X^Z]*AWaCUT
7J s
812-8581 <#/@
6-10-1R !
3I;?)R R (6 $R
PGs(092) 642-2731R
FAXs(092) 642-2715
E-mail: [email protected]
http://seitai.csj.jp/div_sympo/
s
819-0395 <#E 744 8R !";?O&5KMR R 20
PG/FAXs(092)802-2850
E-mail: [email protected]
http://seitai.csj.jp/div_sympo/
R
RR RR
R
R
{/!?ĕĆęÈ È No.30 (2009 June) 42
http://www.geocities.jp/y_glycosci/
nçÊ½¶æ!?ĘK?Ę{!?Ę{y?õE¯ãÛó^Òðé?{æ
væ
âÛËisç
væäÐqÌä¸æåðòÊ±õ?Óà;¤å«âÕôèãYßá
ÒòëÛËæ_V¬wõÒÕÚÝWÊ¬Næ{bõÒ4ìÚÝ[£Ê$åðó(Â%çď
ĄĆęěUTçFêXÙÐË?ãçäòëÛæâÊ¸æbåïz¥âÕóðÑ
åÒÄÐÚëÛËĜÊBç®DâÛËëÝÊoåÒ
îæbâÚÝñ31æ©eâïpÐëÜöË
ÆæÏóæãÚáæ$ïr´ÚáÒòëÛË±õýęĖęĈåaÝäõH¼ÚÊaÚ
Ðõ6ÑÕßÓÖåäôèQÐâÛËgÁÊÒt²åX$ÙÐËÈ
ĥFCCA
dhĥġğğĤPģjġģdě¹ĜīġĤdě5Ĝ
9ĥàóëÐČĒāěZ
ÅĜÈ ġ ¡A
Z-)GLf0"ÇĠġ~Ģġ+ěÀ§ĥ(052)733-1141Ĝ
ěhttp://www.ailabor.or.jp/tmplaza/Ĝ
BĥÈĠĞ_V¬wÈ
È
ºmS=È {ě·<ĜÍ÷ėĊĔùėāø÷ĔĄã»ÎÈ
È
|uÈ {ěI¾<ĜÍ¿äpµõkÛóüėþēûĂĈæC,\ÎÈ
È ġĞ^ě?{ï.íĜåðó¬w&éďĄĆęÈ
È ĢĞ
vÈ
Ć÷đćęċĔĥ ĦĠdħ
10:15īÈ '¼>
#È _V¬w
#WÈ (Â
:bÈ ďĄĆęĚ[£
È
Ħġdħ
#È _V¬wĝ(Âě12:00 @Ĝ
@2ĥ50 $°ĥFCCA 2È x`ÊÁ2È 2,000 Ê?{ 1,000 c°}ªĥnĪĩĩĨąĐĉęêæ$çJ(7¹ěhttp://www.gak.co.jp/AN/kkfundJ.htmlĜÓñæc°
æ}ªÔ*âÛË
}³ ĥO\ 21 P 7 j 31 dě¹Ĝ
É(ÂÏóÐçďĄĆęæMlÊÃõe¦æÊ¦æRê E-mail âÒ}Ú
³ìÙÐË
}³ ĥ·<?È {/S?]È |ìÞóěE-mail: [email protected]Ĝ
¨çĎęđčęăěhttp://www.geocities.jp/y_glycosci/ĜâïØ¢åäó×ãÔâÕëÛË
^ĊúęĒđÈ 8È ě·<ĜÊþĒ÷ÿĀ÷ùėĄ^æÈ R |ìÞóě·<Ĝ
@
FIyx{i i No.30 (2009 June) 43
|LedFIHi *}i
+_HPU#S&HM]i
k598rXZOv/pu,W4O$1aVtFIli
|20094.14-j%21!H'Tct+_HPU#"sonq]}i
b R*N_ Q<H*Nwzx{
Y
m228-8555 GED2b 1-15-1
E-mail: [email protected]
0di K+
FIHi
H)i @?HJi *(
m153-8902 0`Cgf 3-8-1
Tel: 03-5454-4458
E-mail: [email protected]
h[ >_ =^
Hi *(
m520-2194i :\E7;A6B3[ 1-5
Tel: 077-543-7469
Fax: 077-543-7483
E-mail: [email protected]
^"0fjÑËÓ No.30 (2009 June) 44
+6+°4Ã»½s-#Â<r& ±·©FBCÑËÓ°sÂF=¡©¤¥µ
¤ÀÀ«D¿¤·®*°K¼°ÆÎÓÌÂ¤¥µ¤® No. 30 Â3ª¾
»®½s-#
%Ãª½µ'mt°K®±A¢¿£¿¤³Ãv!W
2°{
ÂG¤¥¯©gÂ`µ
5ª±R½¤®¸Á¼ ¿µª´«Ã¬¼¸¼ )°U~Â@
©½µ¤¹§«°JLc®Y½µ¤°J9°5°)°±ÃC
ª¿¸acT(°>ªº5±¸±¹]7®½¨¨¾»ª°/o
pÁ½)¶M®±0ÇÓÊÒ¢°ZÂ¾°PÍÐÓÉÑËÓª¸e¼¡©½µ
«½Q+ wÃVPÃ°|ªPfj¾lÖ&ÉÄÉLP^"0ÇÒÎÈÅÏ
Ô2nd Switzerland-Japan Biomolecular Chemistry Symposium ÔSJBCS2009ÕÕªdÃ,§©
¥µ»`µ
SÔNo. 31Õ±}Ã°F=®»½2009910N°bxÂ1©½µÍÐÓÉÑËÓH$
°¤·dÃ¼°yOBznÂ?¦©½µ{sF=µªqÂ¤¥¿²
:ª
8E2196N24L
I
\P+0+0u[i0fji
Ô[email protected]Õ
sF=
} Ô+;k+0Õ
+h_X.Ô++0Õ

Download Report

PDF file - 東北大学

expydoc.com

Your ExpyDoc