Leggere - Accademia della Felicità

A
TCR γδ
TCR αβ
28.9
1.22
20.7
49.2
FS
73.4
CD4
5.9
93.6
39.6
CD8
SSC
102
0.91
103
102
0
0
0
50K 100K 150K 200K 250K
SSC-A
0
50K 100K 150K 200K 250K
SSC-A
10
4
10
3
10
<AmCyan-A>: CD8
3.43
103
10
CD8+
12.9
5
104
10
12.9
3
3m
102
2
0
0
CD8+
1.31
5
102
0
SSC
CD8+
4.56
105
104
1.31
103
0
50K 100K 150K 200K 250K
SSC-A
104
10
0 stim50K
150K 200K 250K
0 stim50K
150K 200K 250K
V3 6m non
extra 100K
abÉCD8+
0 stim
50K
150K 200K 250K
V3 6m non
extra100K
abÉCD4+
V3 6m non
extra100K
abÉCD4-CD8SSC-A
SSC-A
SSC-A
102
0
CD8+
3.43
105
0
104
7.31
CD4-CD8V3 3m non stim extra abÉCD4-CD8-
104
4.56
103
102
50K 100K 150K 200K 250K
SSC-A
10.6
103
6m
102
0
0
CD8+
10.6
105
<AmCyan-A>: CD8
98.7
103
104
CD4+
7.31
105
<Pacific Blue-A>: CD4
<Pacific Blue-A>: CD4
104
CD4+
0.91
105
1.36
3
102
0
<Pacific Blue-A>: CD4
CD4+
98.7
10
102
0
50K 100K 150K 200K 250K
0 stim
50K
150K 200K 250K
0 stim50K
150K 200K 250K
V3 6m non
extra100K
abÉCD4-CD8V3 6m non
extra 100K
abÉCD4+
V3 6m non stim extra abÉCD8+
SSC-A
SSC-A
SSC-A
105
104
4.28
103
0
0
4
CD8+
1.36
5
<AmCyan-A>: CD8
102
102
CD4+
1.04
103
10
10
CD8+
99
103
104
CD4+
4.28
105
CD8+
V3 3m non stim extra abÉCD8+
V3 3m non stim extra abÉCD4+
<AmCyan-A>: CD8
104
CD4+
1.04
105
<Pacific Blue-A>: CD4
CD4+
99
5
<Pacific Blue-A>: CD4
10
CD4+
CD4-CD8V3 3m non stim extra abÉCD4-CD8-
<AmCyan-A>: CD8
CD8+
V3 3m non stim extra abÉCD8+
<AmCyan-A>: CD8
CD4+
V3 3m non stim extra abÉCD4+
<Pacific Blue-A>: CD4
B
0
0
50K 100K 150K 200K 250K
SSC-A
0
50K 100K 150K 200K 250K
SSC-A
Supplmentrary Figure S 1 A. Gating strategy in PBMCs from patients after aHSCT. DN T-cells were first gated on TCRγδ- T-cells, then on TCRαβ+ T-
cells, followed by CD4+ and CD8+ gating. B. CD4+ , CD8+ or CD4-CD8- T-cells were fixed with PFA and intracellular staining was performed for CD4
molecules (left panel) or CD8 molecules (right panel) coupled with a different dye as compared to the cell surface staining. Cells were obtained from a
patients 3 and 6 month after aHSCT. CD4+ contain intracelluar CD4 molecules, yet low CD8. CD8+ T-cells contain intracellular CD8. CD4-CD8- T-cells
contained intracellular CD4+ as well as CD8 molecules. SS = side scatter

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