Cell Reports, Volume 7 Supplemental Information Free Radicals Mediate Systemic Acquired Resistance Caixia Wang, Mohamed El-Shetehy, M.B. Shine, Keshun Yu, Duroy Navarre, David Wendehenne, Aardra Kachroo, and Pradeep Kachroo A" #Mock# #avrRpt2( #Mock# #avrRpt2( Fig."S1" #α'NOA1# #Ponceau# 24#h# B" DAF'FM#DA# 48#h# DS'RED2# Transmission# Merged# Mock# avrRpt2( (6#h)# Local# avrRpt2( (12#h)# avrRpt2( (24#h)# avrRpt2( (6#h)# avrRpt2( (12#h)# avrRpt2( (24#h)# Distal# NO"(Rela1ve"fluorescence)" C" Fig."S1" 12# 10# 8# *# 6#h# 12#h# 24#h# 6# *# 4# *# 2# 0# Mock# avr( Local# D" Mock# Col'0# avrRpt2( Col'0# avrRpt2( noa1( avrRpt2( rbohD( avrRpt2( rbohF( avrRpt2( sid2( Cu'FL# DS'RED2# *# *# Mock# *# avr( Distal# Transmission# Merged# Fig."S1" E" Col'0# #####nox1( DAF'FM#DA# DS'RED2# Transmission# Merged# A" DS'RED2# DAF'FM#DA# Transmission# Fig."S2" Merged# SULFO'# NONATE# (24#h)# Local# DETA'# NONATE# (24#h)# DETA'# NONATE# (48#h)# SULFO'# NONATE# (24#h)# Distal# DETA'# NONATE# (24#h)# B" NO"(Rela1ve"fluorescence)# DETA'# NONATE# (48#h)# 4# *# SULFO'NONATE# DETA'NONATE# 3# *# 2# C" #SULFO# 24# 48# #DETA# 12# 24# #avr( 48# 24# PR51( 1# #rRNA# 0# Local# Col'0# Distal# D" DAF'FM#DA# Transmission# DS'RED2# Merged# Fig."S2" Mock# Col'0# #######Mock# noa1(nia2( Local# avrRpt2( Col'0# ((((avrRpt2( noa1(nia2( avrRpt2( Col'0# Distal# E" NO"levels"(Rela1ve"fluorescence)# ((((avrRpt2( noa1(nia2( 10# 8# *# Col'0# noa1(nia2( 6# *# 4# 2# 0# MgCl2# avrRpt2( Local# MgCl2# avrRpt2( Distal# Fig."S2" F" ModelAA" ModelAB" ModelAC" Pathogen# Pathogen# Pathogen# SA# NO# NO# SA# SAR# SAR# NO# SA# SAR# 6.8# 5.8# 7.8## avrRpt2( SA'#local# 3.8# 2.8# 1.8# noa1( nia2## Log"(CFU/leaf"disc)" C" avrRpt2( MeSA+#avrRpt2# 4.8## 3.8## 1.8## Col'0# 4.8# 3.8# noa1(nia2( Col'0# noa1(nia2( 0#dpi# 6.8# 5.8# *#*# *# MeSA#(Local)# 5.8## 2.8## noa1( nia2## 3#dpi# Col'0## 0#dpi# MgCl2# 6.8## ** 4.8# Col'0## Fig."S3" B" MgCl2# Log"(CFU/leaf"disc)" Log"(CFU/leaf"disc)" A" 7.8# MgCl2# * * * avrRpt2( 3#dpi# D" Water# PR51( SA'#sprayed# #rRNA# Water# 2.8# SA# 1.8# Col'0## noa1( nia2## Col'0## 0#dpi# F" 3#dpi# 7.8# 7.8# 6.8# 5.8# Log"(CFU/leaf"disc)" Log"(CFU/leaf"disc)" E" noa1( nia2## SULFO'NONATE# DETA'NONATE# * 4.8# 3.8# 6.8# 5.8# * * ** 4.8# * 3.8# 2.8# 1.8# 2.8# 1.8# 0#dpi# 3#dpi# MgCl2# SULFO'NONATE# avrRpt2( DETA'NONATE# Water# SA# SA+SULFO# SA+DETA# G DAF'FM#DA# Transmission# DS'RED2# Fig."S3" Merged# Col'0# avrRpt2( sid2( avrRpt2( *#*# 180## H2O2""(μmol/mg"protein)" J" K" MgCl2'#Local# MgCl2'#Distal# 150## 120## I" rbohD5M( rbohD5avr( rbohF'M( rbohF5avr( avrRpt2'#Local( avrRpt2'#Distal( *#*# 90## #SA# PR51( #rRNA# #rRNA# 14# *# 13# 30## *# 12# 11# 10# 60## #Water# PR51( 4APOBN"Free"Radical"Adduct" Forma1on" Col'0# H" LOCAL# 9# DISTAL# 8# 0## 24#h# 12#h# Col'0# 12#h# 24#h# noa1(nia2( 0hpi# 6hpi# 12hpi# 24hpi# 80# %"4APOBN"Adduct"aSer"Avr" Infec1on" L" Fig."S3" 60# 40# 20# 0# '20# Local# M" DAF'FM#DA# DS'RED2# Transmission# Distal# Merged# Mock# Col'0# Mock# rbohF( avrRpt2( Col'0# Local# avrRpt2( rbohF( N" Water# ######H2O2# #α'NOA1# avrRpt2( Col'0# #Ponceau# Distal# avrRpt2( rbohF( Fig."S4" A" AA'ME# B" 70000# DPM/"g"FW" 60000# 50000# 40000# 30000# 20000# 10000# 0# Local# Distal#
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