Ammonia breath analyzer demonstrator Dr.Ir. Marieke Snijder-van As 02-04-2014 Page 1 Relitech – co-creator 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 2 ABA demonstrator 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 3 NH3 in the human body (1) Wu et al, 2013, Molecules 18(4) 4689-4702 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 4 NH3 in the human body (2) Emily Hays – physio-pedia.com Wikimedia Commons 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 5 Measurement principle Air sample flow Demi water flow Conductivity sensor + NH3 NH4 + NH4 Acid flow (pH 3.5) 02-04-2014 Base flow (pH 12) Relitech – Marieke Snijder-van As – Sense of Contact 16 Waste Page 6 Design 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 7 Characterisation & optimization 100 3500 80 3000 60 2500 40 2000 1500 0 2000 4000 Time (s) 6000 8000 Sample tube temperature 40 40 38 38 02-04-2014 150 41 0.3 0.2 40 0.1 500 1000 1500 Time (s) 2000 39 2500 Multiple measurements of 60 ppb sample 650 Concentration (adc values) 42 Temperature (C) Temperature (C) 46 42 50 100 Distance from connector (cm) 0.4 700 44 0 0.5 -0.1 0 20 44 42 0 Sample tube temperature 46 Temperature stability 0.6 NH3 concentration (ppm) 4000 Demi-water flow (ul/min) NH3 concentration (ADC values) 120 Temperature climate chamber (C) Demi-water flow stability 4500 600 550 500 450 400 28 30 32 34 Time (min) Relitech – Marieke Snijder-van As – Sense of Contact 16 36 38 40 Page 8 NH3 during exercise Workload [W] NH3 concentration [ppm] Exercise experiment 15 0.2 0.1 100 100 5 50 0 0 02-04-2014 Heart rate [bpm] Lactate [mmol/l] 200 150 10 5 10 15 Time [min] 20 Relitech – Marieke Snijder-van As – Sense of Contact 16 25 0 Page 9 NH3 during exercise Exercise experiment 15 Heart rate [bpm] Lactate [mmol/l] Workload [W] NH3 concentration [ppm] 0.8 200 150 10 0.4 100 100 5 50 0 0 02-04-2014 5 10 15 20 Time [min] 25 Relitech – Marieke Snijder-van As – Sense of Contact 16 30 0 35 Page 10 NH3 in stomach ulcer Helicobacter Pylori Experiment 0.8 NH3 (ppm) 0.6 0.4 0.2 0 40 02-04-2014 60 80 Time (min) 100 Relitech – Marieke Snijder-van As – Sense of Contact 16 120 Page 11 Conclusions ABA Demonstrator specifications: • • • • Sensitivity: 60 -100 ppb Measurement range: 0.1-10 ppm Response time: 10-15s Standard deviation: 5% Bench-top application of microfluidics gives challenges Keep checking science and literature Co-creation is essential 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 12 Co-creation Innovative solutions created by the teamwork of involved people 02-04-2014 Relitech – Marieke Snijder-van As – Sense of Contact 16 Page 13
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