Supplementary Table 1 siRNA oligonucleotides siXEDAR sip53 siFAS siEGFP Sense GCCUGCUAGUGGUGUUUACTT GACUCCAGUGGUAAUCUACTT GAAGCGUAUGACACAUUGATT GCAGCACGACUUCUUCAAGTT Antisense GUAAACACCACUAGCAGGCTT GUAGAUUACCACUGGAGUCTT UCAAUGUGUCAUACGCUUCTT CUUGAAGAAGUCGUGCUGCTT Quantitative real-time PCR (SYBR Green I Master) XEDAR b2-microglobulin Forward GGTCCTGGACAGGAGCTATC TCTCTCTTTCTGGCCTGGAG Reverse ACGATTGATGACAGCACAGG AATGTCGGATGGATGAAACC Quantitative real-time PCR (Probes Master) XEDAR b2-microglobulin Forward CCAACTTTTTGAGGTTCAGCA TAGGAGGGCTGGCAACTTAG Reverse TCACTTCACCTTTACCCGAAA CCAAGATGTTGATGTTGGATAAGA ChIP assay p53BR1 p53BR2 Forward GCGCTTTCTGAGGTATCAGG GCAAAGCAAGAGGTTTCAGG Reverse TTTCCTAGCCTGGTCTCTGG ATGCGTACGTGCACAAAGAC Gene reporter assays p53BR1 p53BR2 Forward AAAACGCGTGCGCTTTCTGAGGTATCAGG AAAACGCGTGCAAAGCAAGAGGTTTCAGG Reverse AAACTCGAGTTTCCTAGCCTGGTCTCTGG AAACTCGAGATGCGTACGTGCACAAAGAC Bisulfite sequencing analysis Forward CpG island in the XEDAR promoter GGAATTTTGATTATAAGGAATTTTATATAT Reverse ACACCAAAAAATATTCCCACACT Semi-quantitative RT-PCR XEDAR b2-microglobulin Forward TCTTCAACAGACATTGCCAG CACCCCCACTGAAAAAGATGA Reverse CAGTATAGGAGGCAGAGCTG TACCTGTGGAGCAACCTGC Mutation analysis in p53 gene p53_115F p53_596F p53_634R p53_1004F p53_1052R p53_1522R AAGTCTAGAGCCACCGTCCA TTCTGGGACAGCCAAGTCTG AAACATCTTGTTGAGGGCAG CCTCACCATCATCACACTGG CACGCACCTCAAAGCTGTTC CAAGGGTTCAAAGACCCAAA Mutation analysis in XEDAR gene exon 1 exon 2 exon 3 exon 4 exon 5 exon 6 Forward AAGCCAAGTGGCATGGAAG ACAGGTGATCCCATCTCCAA CCAAGTGCATTGCTTGTTTG TGACTGTTTACTGCCCATGC GGCAAGGGAGATGGTATCAA GGAAAGAATCAGCTCCCAGA Reverse GTTTTACCCGCTGAACTGGA GATGGGAGAAACCTCCAACA GGGATGAGGAGAAAGGGAAG ACCCCAGAACATCCCTCTCT AAACCTCCTGCAACTCGAAG ATGACAACCTTGCCAGTTCC Supplementary Table 2 No p53 status 1 mt 2 mt 3 mt 4 mt 5 mt 6 mt 7 mt 8 mt 9 mt 10 mt 11 mt 12 mt 13 mt 14 wt 15 wt 16 wt 17 wt 18 wt 19 wt 20 wt No 1 2 3 4 5 6 Colon cancer cell lines DLD-1 HCT-15 HT-29 KM12C KM12SM NCI-H508 NCI-H716 SNU-C2A SNU C5 SW480 SW620 SW948 WiDr HCT 116 LoVo LS 174T NCI-H498 RKO SNU C4 SW48 Normal cell lines HEK293 HMEC HUVEC NHDF NHEK SAEC No 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 No p53 status 1 mt 2 mt 3 mt 4 mt 5 mt 6 mt 7 mt 8 mt 9 mt 10 mt 11 mt 12 mt 13 mt 14 mt 15 mt 16 mt 17 wt 18 wt 19 wt 20 wt 21 wt 22 wt Normal tissues Brain Heart Liver Lung Kidney Stomach Pancreas Testis Placenta Prostate Bone Marrow Spleen Trachea Thymus Colon Small Intestine Ovary Bladder Mammary Gland Spinal Cord Lymph Node Skeletal Muscle Thyroid Pituitary Gland Aorta peripheral blood Breast cancer cell lines MDA-MB-157 MDA-MB-435S MDA-MB-453 OCUB-F BT-474 BT-20 BT-549 HCC1395 HCC1143 HCC1599 HCC1937 SK-BR-3 T-47D BSY1 HBC5 MDA-MB-231 (HTB-26) HCC1500 ZR-75-1 YMB-1 HBC4 HBL-100 MCF7 No p53 status 1 mt 2 mt 3 mt 4 mt 5 mt 6 mt 7 mt 8 mt 9 mt 10 mt 11 mt 12 mt 13 mt 14 mt 15 mt 16 mt 17 mt 18 mt 19 mt 20 mt 21 mt 22 mt 23 mt 24 mt 25 mt 26 mt 27 mt 28 wt 29 wt 30 wt 31 wt 32 wt 33 wt 34 wt 35 wt Lung cancer cell lines DMS 114 DMS 273 LC174 NCI-H358 NCI-H522 Pc-3 SK-LU-1 SK-MES-1 PC14 PERF-LC-AI NCI-H1703 EBC-1 LU61 SBC-5 NCI-H1299 NCI-H446 NCI-H196 NCI-H1650 NCI-H1781 NCI-H2170 NCI-H23 NCI-H520 NCI-H647 LX1 PC14PE6 RERF-LC-AI SW900 A549 LC176 LU99A LU99B NCI-H460 SBC-3 A427 SW1573
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