Current Status of Varietal Improvement in Specialty Rice Suitable for Food Processing and High-Functional Utility National Institute of Crop Science Hae Chun, Choi 7.0 6.5 6.0 5.5 5.0 4.5 4.0 3.5 3.0 2.5 Rice production Cultivated area 1960 1970 1980 1990 1700 1600 1500 1400 1300 1200 1100 1000 900 2000 Year Fig. Changes in amount of rice production and cultivated area during the last four decades in Korea 150 Self-sufficiency ratio Milled rice consumption per capita 140 130 120 110 100 90 1965 1970 1975 1980 1985 1990 1995 200 180 160 140 120 100 80 60 40 20 0 2000 Year Fig. Changes in milled rice consumption per capita and self-sufficiency ratio in rice production during the last four decades in Korea Grain length (Brown rice:min) Grain length/ width ratio (Brown rice) 3.3 1.10 4.6 1.63 6.2 9.5 2.60 4.50 1,000-Grain weight(g) 10 17 35 46 Pericarp color Light Light Light Red yellowish straw Brown Brown white Dark red Purple brown Purple Black purple Degree of chalkiness(0-9) 0 1 3 7 9 Fig. Varietal variation of grain appearance in world collection and Korean rice varieties : Varietal range of Korean rice Aroma Very weak Alkali Digestion (1-7) Gel consistency(mm) Weak 1 20 30 Fair 2 40 Considerable 3 50 4 5 60 70 Strong 6 80 90 7 100 Amylose content(%) 0 10 Protein content(%) (Brown rice) 6 Lipid content (%) (Brown rice) 2.3 6.5 16 21 27 11 3.0 35 15 3.6 Fig. Varietal variation of major physicochemical properties of milled or brown rice in world collections and Korean improved rice cultivars : Varietal range of Korean rice Various shape of seed rice Various color and shape of colored rice Table . Major characteristics of newly-developed specialty rice cultivars suitable for food processing and functional utility . Cultivar Bred Heading Culm Milled 1000- Brown WC Pericarp Aroma Amylose rice grain rice length /WB yield weigh L/W color (0∼9) (%) (cm) (0∼9) (t/ha) (g) ratio year date Daeribbyeo 1 ’93 Aug.15 88 4.45 34.8 1.94 1/0 YW 0 19.5 Hyangmibyeo 1 ’93 Aug.15 72 4.93 20.6 2.46 1/1 〃 5 18.3 Hyangnambyeo ’95 Aug.11 82 5.03 21.3 1.81 0/0 〃 3 17.7 Hyangmibyeo 2 ’96 Aug.4 77 6.14 22.8 2.44 1/2 〃 3 19.0 Yangjobyeo ’94 Aug.14 71 5.11 25.4 1.78 7/0 〃 0 20.2 Arranghyangchalbyeo ’97 Aug.13 88 5.37 20.5 1.90 wx 〃 3 0 Heugjinjubyeo ’97 July 25 80 4.05 17.0 2.22 - BP 1 15.1 Heugnambyeo ’97 Aug.13 73 4.97 23.5 2.14 - 〃 1 16.7 (Continued) Cultivar Bred Heading Culm Milled 1000- Brown length rice grain rice yield weigh L/W (cm) (t/ha) (g) ratio WC Pericarp Aroma Amylose /WB color (0∼9) (%) (0∼9) year date ’98 Aug.16 79 5.57 22.6 1.86 0/0 YW 3 19.0 Seolhyangchalbyeo’99 ’00 Goamibyeo Aug. 8 89 5.23 24.2 2.10 wx 〃 3 0 Aug.18 85 5.38 20.8 1.77 0/2 〃 0 26.7 Heughyang ’00 Aug.23 77 5.28 23.1 1.96 - BP 3 18.0 Jeogjiniu ’00 July 26 81 5.54 22.1 1.79 - RB 0 18.3 Baegjinju ’01 Aug.21 77 5.18 19.2 1.62 dull YW 0 9. 1 Seolgaeng ’01 Aug.19 83 5.27 20.3 1.63 opaque YW 0 19.3 Yeongan ’01 Aug.13 83 5.45 22.4 1.79 0/1 〃 HL 18.9 Goami 2 ’02 Aug.22 75 4.24 14.6 1.89 4/4 〃 HF 28.1 Manmi ’02 Aug.17 86 4.46 19.9 1.84 0/0 〃 0 12.9 Heuggwang ’03 Aug.14 89 5.05 14.8 1.73 - BP 0 18.0 Mihyangbyeo WC/WB : White core/ White belly, YW : Yellow white, BP : Black purple, RB : Reddish brown , HL : High lysine, HF : High fiber Table . Cultivation area of specialty rice cultivars during ’96∼2000 Main trait Cultivation area (ha) Major rice cultivars '96 '97 '98 '99 2000 13,515 11,983 Waxy Shinseonchal, Hwaseonchal 15,324 13,138 14,173 Aromatic Hyangmibyeo 1, Hyangnam 45 163 387 800 103 Heugjinju, Heugnam 2 2,153 1,726 1,103 727 Aranghyangchal, Seolhyangchal 2 - 11 118 140 15,373 15,454 16,297 1.5 1.5 1.5 Colored Aromatic waxy Total area (ha) Proportion ratio to total rice cultivation area (%) 15,536 12,953 1.5 1.2 Daeribbyeo 1 Nagdongbyeo Yongjubyeo IR841-76-1 Daeribbyeo 1 IR41999 T(N) 1 (H.Amy, H.GC, I) Suwonjo (H.Amy, M.GC, J) Hangangchalbyeo (Waxy, L.GC,I) Nagdongbyeo (L. Amy, L.GC,J) Fig. Varietal difference in cross-sectional figure of popped rice kernel observed through the scanning electron microscope (H : High, L : Low, Amy : Amylose, I : Inidica, J : Japonica). Table. Correlation coefficients between popping characteristics and morphological or physicochemical properties of rice grain Relevant characters Chalkiness (WB or WC) Translucency Hardness Gel consistency 〃 〃 Amylose content 〃 〃 〃 - Ratio of intact popped brown rice - 〃 Ratio of popped rice Bulk density of popped brown rice Bulk density of popped milled rice Ratio of popped rice Bulk density of popped milled rice Ratio of popped rice Expansion rate in length of milled rice Expansion rate in volume of milled rice WB : white-belly, WC : white-core in rice grain Correlation coefficients -0.585** -0.456* 0.472* -0.706** -0.776** 0.792** 0.512* -0.484* -0.477* -0.475* Table. Correlation coefficients between suitability for rice noodle and physicochemical properties of milled rice Relevant Characters K content Mg content Extracted solid amount Doughing time Toughness of dried noodle - Gross score of noodle making 〃 〃 - Acceptability to noodle formation 〃 *, ** : Significant at 5% and 1% levels, respectively. Gross score of noodle making is mean estimate of acceptability to noodle formation and toughness of dried noodle. Correlation coefficients -0.603* -0.553* -0.522 0.905** 0.798** Table. Relationships between some physicochemical properties of rice grain and cooked rice noodle characteristics Relevant characters Correlation coefficients Solid amount of cooking extract - 0.819 ** ˝ 0.720 ** 0.712 ** Turbidity of noodle-boiled water - Solid amount of cooking extract - Amylose Gel consistency Potassium Amylose Gel consistency Potassium Magnesium ˝ Total acceptability of cooked noodle - 0.717 ** ˝ 0.700 ** ˝ ˝ 0.677 ** ˝ 0.459 ˝ 0.800 ** 0.547 * *, ** : Significant at 5% and 1% levels, respectively. Total acceptability of cooked noodle is average acceptability of tensility and palatability of cooked noodle, and turbidity and solid amounts of noodle - boiled water. Eating quality of cooked noodle 9 Hangangchal Taebaek Nampung 7 Suweonjo S.232 5 BG276-5 Hanyangjo IR44593 San-Li-Cun Chokoto 3 1 Pusa-33-30 IR44 Wheat 1 3 5 7 9 Acceptability to noodle - making Fig. Distribution of rice varieties based on total acceptability to noodle - making and eating quality of cooked noodle. White rice Brown rice Hangangchalbyeo White rice Brown rice IR841-76-1 White rice Brown rice Suwon230 White rice Brown rice AC 27 White rice Brown rice Suwon232 White rice Brown rice Suwonjo Fig. Traditional difference in processing quality for rice bread, (A) : milled rice, (B) Brown rice. Table. Correlation coefficients among chemical properties of rice grain and various characteristics related to processing quality of rice bread Relevant characters Protein content Fat content Expansion of batters Amylose content Gel consistency Alkali digestibility - Moisture of rice bread - Uniformity of porous cell size in rice bread - Size of porous cell in rice bread - Springiness of rice bread - Loaf formation of rice bread - Size of porous cell in rice bread - Springiness of rice bread - Springiness of rice bread - Springiness of rice bread - Retrogradation of rice bread 〃 *, ** : Significant at 5% and 1% levels, respectively. Correlation coefficients Brown rice Milled rice -0.547 0.537 -0.783* 0.824* -0.637 -0.351 0.859** 0.301 0.761* -0.898** -0.875** 0.720* 0.703* 0.204 - 0.571* -0.652* 0.572* -0.625* Beckjinju (half-glutinous-rice) Shyulgang Suwon 428 (white-nonglutinous-rice) (glutinous-rice) Ilpumbyeo (white-nonglutinous-rice) Ilpumbyeo Mutant Special Rice [Top is Brown Rice, bottom is white rice, middle is iodine tested] Table. Brewing characteristics of some specialty rice varieties Koji quality Variety Saccharogenic Mycelium power density (mg glucose/hr/g) and flavor Dasanbyeo Daeripbyeo 1 Baegjinju Seolgaeng Yangjobyeo Ilpumbyeo 32.8 30.1 41.2 54.8 31.2 30.4 Good Vary good Good Very good Very good Good Mash quality Acidity (0.1N -NaOH㎖ /10㎖) 3.2 3.1 2.8 2.8 3.4 3.5 Sensory Amino acidity Alcohol accepta(0.1N -NaOH㎖ bility (%) /10㎖) 0.6 0.8 0.9 0.8 0.7 0.6 17.2 17.0 17.1 17.0 16.4 16.9 Good Very good Excellent 〃 Very good Good Table. Mycelia rooting density and saccharogenic power during the processing of fermented rice using Aspergillus oryzae Variety Baegjinju Selgaeng Ilpumbyeo Endosperm characteristics milky, amylose ; 9% opague, amylose ; 19.3% translucent, amylose ; 18.9% Rooting density of mycelia Saccharogenic power (mg/glucose/hr/g) Brown rice Milled rice Brown rice Milled rice ++ ++ 37.4 38.2 +++ +++ 41.6 45.4 ++ +++ 39.3 42.6 Table. Pigment concentration of fermented rice using Monascus anka Absorbance Variety Baejinju Selgaeng Ilpumbyeo 400nm (yellow) 470nm (orange) 500nm (red) 7.32 13.72 14.34 4.21 11.50 9.31 10.42 19.70 14.84 Table . Varietal variation in frequency ratios among short, intermediate and long glucose chains composing amylopectin structure of glutinous rice endosperms. Trait Mean Fr. II (%) (Fr.III+IV)/Fr. II Fr. IV/Fr. II Fr. III/Fr. II Fr. IV/Fr. III 18.6 4.47 1.78 2.70 0.68 Standard deviation Coefficients of variation (%) Range 2.684 0.729 0.460 0.505 0.234 14.4 16.3 25.9 18.7 34.3 14.7∼25.2 2.97∼5.79 1.09∼2.72 1.42∼3.59 0.41∼1.31 Fr. II, III and IV are the long, intermediate and short glucose chains of amylopectin, respectively. Z2 1.0 II 38 2 VIII III 0.5 35 1 3 -0.5 34 I 30 21 33 10 16 15 37 20 22 19 9 -1.0 14 17 V 18 23 -1.5 36 IX 28 25 4 11 12 IV VI 5 0.0 0.5 31 8 7 24 13 -0.5 1.0 1.5 2.0 Z1 6 32 29 VII 27 -1.0 26 -1.5 Fig. 1. Scatter diagram of tested glutinous rice cultivars on the plane of 1st (Z1) and 2nd (Z2) principal conponents contracted from eleven qulity properties. (○ : Japonica, ● : Indica, ▲ : Javanica) Table . Correlation coefficients between some indicaters of amylopectin structure and other physicochemical properties of glutinous rice Relevant Characters Fr. II (%) Fr. III (%) Fr. IV (Fr. IV+III) Fr. IV /Fr.II /Fr. II (%) Fr. III /Fr. II Fr. IV /Fr. III WBA 0.492* 0.054 -0.270 -0.197 -0.476* -0.441* -0.284 -0.151 GVE 0.467* 0.009 -0.453* -0.381 -0.307 -0.105 Breakdown -0.339 0.657** -0.498* 0.340 -0.216 0.687** -0.541* Setback 0.376 -0.683** 0.509* -0.369 0.206 -0.719** 0.568** Adhesiveness -0.234 0.430* -0.322 0.208 -0.153 0.439* -0.372 Balance 0.543* -0.417 0.246 -0.206 0.541* -0.473* -0.275 *, ** : significant at 5% and 1% levels, respectively. Table . Suitability of glutinous rice for processing oil-puffed rice cake Variety Bulk density Hardness Sensory evalutaion (1∼5) (㎖/g) (kg) Crispness Total IR29 2.880 0.810 2.10 2.45 Hangangchalbyeo 2.930 1.030 1.80 1.90 Malakitsinaguing 4.525 0.806 3.25 2.75 Onnemochi 5.985 0.705 3.90 3.35 Gangwonna 2.900 0.382 4.25 3.80 Rikuto Norinmochi 1 5.120 0.220 3.70 3.90 Jinbuchalbyeo 5.950 0.668 3.00 2.60 Hwaseonchalbyeo 7.665 0.644 4.05 3.85 Ilpumbyeo (Nonwaxy) 2.435 1.684 1.70 1.45 Table . Correlation coefficients between some physicochemical properties of glutinous rice grain and suitability for processing an oil-puffed rice cake Relevant characters Gel consistency Correlation coefficients - Water absorption 0.690** - Water absorption 0.635* 〃 - Reducing sugar 0.616* 〃 - Crispness 0.863** Sensory evaluation Total *, ** : significant at 5% and 1% levels, respectively. Various sugary Rice Ordinary Rice Ordinary Rice Huge whole-rice 㐘 用途 and 機能性 多樣化 㐘 粒大 粒形 種皮色 香臭性 理化學的 特性 小粒 ~ 大粒, 短圓 ~ 細長, 黃褐 ~ 黑紫, 低香 ~ 高香 低 ~ 高 糊化溫度 , 低 ~ 高 amylose, 低 ~ 高 蛋白質, 低 ~ 高 脂肪 低 ~ 高 纖維質 等 澱粉構造 and 健康機能性 突然變異 誘發 and 生物工學 技法 活用 Amylopectin 構造 變更, 澱粉形態 變更 低 앨러진 , 高 glycine, 高 微量元素 高 各種 Vitamin, 高 各種必須 Amino acid 巨大胚芽米 , 糖質, 粉質, 高食餌纖維 etc 㐘 食品 and 健康 機能性 of 多樣化 開發 資源變異 擴大
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