5'- AAAAAA(A)n-3' ↓ 1st strand synthesis by Reverse transcriptase 5'- AAAAAA(A)n-3' 3'- TTTTTT ↓ Removed RNA by NaOH 3'- TTTTTT -5' -5' ↓ 2nd strand synthesis by Taq DNA polymerase 5'9N1B 3'- AAAAAA -3' TTTTTT -5' ↓ Amplification of PCR by Taq DNA polymerase 5'- AAAAAA -3' 3'- TTTTTT -5' ↓ Cloning into T-vector ↓ Sequencing Homology search for database
© Copyright 2024 ExpyDoc
