A
WT
(h)
0
2
bub2Δ
4
6
(h)
Securin
Securin
CDK
G6PDH
B
CEN-GFP
bub2Δ
securin-db
bub2Δ
securin-dkb
C
%
0
DAPI
1
bub2Δ cdh1Δ
3
5
Merge
0
1
3
DIC
5