Recovery of the genetically selected broiler line
by transferring cryopreserved circulating PGCs.
Atsushi Tajima 1) 2), Guy F. Barbato3), Palmer G. Cramer1),
Carol H. Crouch 3) , Takashi Kuwana 4), Roy H. Hammerstedt 1)
1) Department of Biochemistry and Molecular Biology,
Penn State University, University Park, PA, 16802, USA.
2) Institute of Agriculture and Forestry,
,
University of Tsukuba, Ibaraki 305-8572,
Japan.
3) Department of Poultry Science.
Penn State University, University Park, PA, 16802, USA.
4) National Institute for Minamata Disease,
Kumamoto 867-0008, Japan.
Materials and Methods 1
Fertilized Eggs
Incubation
(42L)
(37.8 oC)
Blood collection (Stage 13-15)
Freezing
LN2
Bicell (Nihon Freezer)
10% DMSO,
-80 oC
Materials and Methods 2
Brown Leghon (BL) Eggs
Dekalp, IL
Incubation (37.8 oC)
Blood removal (stage 13-15)
Injection
Hatch
Progeny testing
Thaw blood
(Ice water 4 oC)
Isolation of PGCs
Remove DMSO
Results
Female BL(42L)
Batch
Total number of
chick hatched
Number of
white chick
1
4
4
100.0
2
2
1
50.0
3
5
3
60.0
White chicks
%
Results
Male BL(42L)
Batch
Total number of
chick hatched
Number of
white chick
White chicks
%
1
12
6
50.0
2
21
10
47.6
Conclusion
A broiler line (42L) was recovered using
frozen/thawed circulating PGCs.
Genetically important lines (grand parents, transgenics etc)
can be recovered using frozen/thawed PCGs
Acknowlegments
Dr. Lary Vint (DeKalp)
for providing Brown Leghorn eggs
Financial support
Ministry of Education, Science, Culture
and Sports, Japan.
Ministry of Environment, Japan.

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