IHC on DISCOVERY ULTRA Research Instrument
EZH2 (SP129), with DISCOVERY HQ-HRP Detection and DISCOVERY Amp HQ Kit in tonsil
Reagents Needed from Ventana
Name
Catalog number
Ordering code
DISCOVERY Anti-Rabbit HQ
760-4815
7017812001
DISCOVERY Anti-HQ HRP
760-4820
7017936001
DISCOVERY ChromoMap DAB Kit
(RUO)
760-159
5266645001
DISCOVERY CC1
950-500
6414575001
Hematoxylin II
790-2208
5277965001
Bluing Reagent
760-2037
5266769001
EZH2 (SP129) Rabbit Monoclonal
Primary Antibody
790-4651
6522858001
DISCOVERY Amp HQ Kit
760-052
6472320001
DISCOVERY Amplification anti-HQ HRP
Multimer
760-4602
6442544001
IHC with DISCOVERY HQ-HRP Detection and DISCOVERY Amp HQ Kit
RUO DISCOVERY Universal Staining procedure for DISCOVERY ULTRA Research
Instrument
1. Deparaffinization [Selected]
23. Linking Antibody [Selected]
2. Warmup Slide to [69 Deg C], and incubate
for [8 Minutes] (Cycle 1)
24. 2nd Antibody [Selected]
3. Incubate for [8 Minutes] (Cycle2)
4. Incubate for [8 Minutes] (Cycle3)
5. Pretreatment [Selected]
6. Cell Conditioning [Selected]
25. Warmup Slide to [36 Deg C] from Very Low
Temperatures (2nd Antibody)
26. Apply One Drop of [Anti-Rabbit HQ]
(Detection #1), and incubate for
[0 HR 12 Min]
27. Enzyme conjugate [Selected]
7. CC1 Resivoir [Selected]
8. Warmup Slide to [93 Deg C], and incubate
for 4 Minutes (Cell Conditioner #1)
28. Apply One Drop of [Anti-HQ HRP]
(Conjugate #1), and Incubate for
[12 Minutes]
9. CC1 8 Min [Selected]
29. DISCOVERY Amplification [Selected]
10. CC1 16 Min [Selected]
30. DISCOVERY Amplification HQ [Selected]
11. CC1 24 Min [Selected]
31. Apply One Drop of DISC AMP TSA HQ and
One Drop of DISC AMP H2O2 HQ, Incubate
for [4 Minutes]
12. CC1 32 Min [Selected]
13. CC1 40 Min [Selected]
32. Apply One Drop of [DISC anti-HQ HRP]
(DISCOVERY AMP Multimer), and Incubate
for [4 Minutes]
14. CC1 48 Min [Selected]
15. CC1 56 Min [Selected]
33. DAB [Selected]
16. CC1 64 Min [Selected]
34. Counterstain [Selected]
17. Inhibitor [Selected]
35. Use RB for Counterstain [Selected]
18. Inhibitor CM [Selected]
19. Apply One Drop of Inhibitor CM, and
Incubate for [12 Minutes]
36. Apply One Drop of [HEMATOXYLIN II]
(Counterstain), and incubate for
[8 minutes]
20. Antibody [Selected]
37. Post Counterstain [Selected]
21. Warmup Slide to [36 Deg C] from Very Low
Temperatures (Primary Antibody)
38. Use RB for Post Counterstain [Selected]
22. Apply One Drop of [EZH2 (SP129)]
(Antibody), and incubate for [24 Minutes]
39. Apply One Drop of [BLUING REAGENT]
(Post Counterstain), and incubate for
[4 minutes]
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