Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements AAV (Adeno-associated) Viral Vectors For Neuroscience Applications Expression Vectors- by gene Griesbeck: Calcium Sensor: Twitch2B AV-1-49531P AAV1.hSyn1.Twitch2B.WPRE.SV40 (Addgene49531P) MTA for original source material Rose/Huebener/Bonhoeffer: Bicistronic Calcium Sensor: mRuby2-P2A-GCaMP6 AV-1-50943 AV-1-50942 AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6f.WPRE.SV40 (Addgene50943) AAV1.hSyn1.mRuby2.GSG.P2A.GCaMP6s.WPRE.SV40 ( Addgene50942) MTA for original source material MTA for original source material GENIE: Calcium Sensor: GCaMP6 AV-1-PV2822 AV-1-PV2824 AV-1-PV2823 AV-5-PV2824 AV-5-PV2823 AV-5-PV2822 AV-9-PV2823 AV-9-PV2822 AV-9-PV2824 AV-1-PV2833 AV-1-PV3081 AV-5-PV2833 AV-9-PV2833 AV-9-PV3080 AV-9-PV3081 AAV1.Syn.GCaMP6f.WPRE.SV40 AAV1.Syn.GCaMP6s.WPRE.SV40 AAV1.Syn.GCaMP6m.WPRE.SV40 AAV5.Syn.GCaMP6s.WPRE.SV40 AAV5.Syn.GCaMP6m.WPRE.SV40 AAV5.Syn.GCaMP6f.WPRE.SV40 AAV9.Syn.GCaMP6m.WPRE.SV40 AAV9.Syn.GCaMP6f.WPRE.SV40 AAV9.Syn.GCaMP6s.WPRE.SV40 AAV1.CAG.GCaMP6s.WPRE.SV40 AAV1.CAG.GCaMP6f.WPRE.SV40 AAV5.CAG.GCaMP6s.WPRE.SV40 AAV9.CAG.GCaMP6s.WPRE.SV40 AAV9.CAG.GCaMP6m.WPRE.SV40 AAV9.CAG.GCaMP6f.WPRE.SV40 GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GENIE: Calcium Sensor: GCaMP5G AV-1-PV2478 AV-5-PV2478 AV-9-PV2478 AV-9-PV2643 AAV1.hSyn.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV5.hSyn.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV9.hSyn.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV9.CAG.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 GCaMP5 acknowledgement GCaMP5 acknowledgement GCaMP5 acknowledgement GCaMP5 acknowledgement Looger: Calcium Sensor: GCaMP3 AV-1-PV1627 AV-5-PV1627 AV-9-PV1627 AAV1.hSyn.GCaMP3.WPRE.SV40 AAV5.hSyn.GCaMP3.WPRE.SV40 AAV9.hSyn.GCaMP3.WPRE.SV40 GCaMP3 acknowledgement GCaMP3 acknowledgement GCaMP3 acknowledgement Looger: Calcium Sensor: RCaMP AV-1-PV3010 AV-9-PV3010 AAV1.Syn.RCaMP1h.WPRE.SV40 AAV9.Syn.RCaMP1h.WPRE.SV40 L. Looger/HHMI acknowledgement L. Looger/HHMI acknowledgement Looger: Glutamate Sensor: iGluSnFr AV-1-PV2914 AV-9-PV2914 AV-1-PV2723 AV-5-PV2723 AV-5-PV2914 AV-9-PV2723 AAV1.GFAP.iGluSnFr.WPRE.SV40 AAV9.GFAP.iGluSnFr.WPRE.SV40 AAV1.hSyn.iGluSnFr.WPRE.SV40 AAV5.hSyn.iGluSnFr.WPRE.SV40 AAV5.GFAP.iGluSnFr.WPRE.SV40 AAV9.hSyn.iGluSnFr.WPRE.SV40 iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement Khakh: Calcium Sensor: GCaMP3 AV-5-44330 AV-5-44331 AV-5-PV3107 AAV5.GfaABC1D.PI.Lck-GCaMP3.SV40 (Addgene44330) AAV5.GfaABC1D.PI.cyto-GCaMP3.SV40 (Addgene44331) AAV5.GfaABC1D.Lck-GCaMP6f.SV40 MTA for original source material MTA for original source material Lagnado: Synaptophysin-Calcium Sensor: synGCaMP AV-1-PV2368 AAV1.CMV.PI.SynGCaMP3.SV40 L. Lagnado/Cambridge acknowledgement Pierebone: Fluorogenetic Voltage Sensor: ArcLightD AV-1-36857P AAV1.hSyn.ArcLightD.WPRE.SV40 (Addgene36857P) MTA for original source material Boyden: Optogenetic Inhibition: Arch and ArchT AV-1-PV2509 AV-9-PV2509 AV-1-PV2527 AV-5-PV2527 5/20/2014 AAV1.CAG.ArchT.GFP.WPRE.SV40 AAV9.CAG.ArchT.GFP.WPRE.SV40 AAV1.CAMKII.ArchT.GFP.WPRE.SV40 AAV5.CAMKII.ArchT.GFP.WPRE.SV40 E. Boyden / MIT acknowledgement E. Boyden / MIT acknowledgement E. Boyden / MIT acknowledgement Page 1 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements AV-9-PV2527 AAV9.CAMKII.ArchT.GFP.WPRE.SV40 E. Boyden / MIT acknowledgement Deisseroth: Red-Shifted Optical Excitation: C1V1(E122T/E162T) AV-9-35499 AAV9.CamKIIa.C1V1(E122T/E162T).TS.eYFP.WPRE.hGH (Addgene35499) MTA for original source material Deisseroth: Stabilized Step Function Opsins [SSFO]: ChR2 (C128S/D156A) AV-5-35501 AAV5.CaMKIIa.hChR2(C128S/D156A)-eYFP.WPRE.hGH(Addgene35501) MTA for original source material Deisseroth: 2nd Generation Ultrafast Optogenetic Control: ChR2(E123A) AV-2-35505 AV-5-35505 AV-9-35506 AV-9-35505 AV-9-35505 AAV2.CamKII.hChR2(E123A)-eYFP.WPRE.hGH (Addgene35505) AAV5.CamKII.hChR2 (E123A)-eYFP.WPRE.hGH (Addgene35505) AAV9.CaMKII.hChR2(E123A).mCherry.WPRE.hGH.(Addgene35506) AAV9.CamKII.hChR2(E123A)-eYFP.WPRE.hGH (Addgene35505) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material Deisseroth: 2nd Generation Ultrafast Optogenetic Control: ChR2 (E123T/T159C) AV-1-35512 AV-9-35512 AV-9-35511 AAV1.CamKIIa.hChR2(E123T/T159C).mCherry.WPRE.hGH(Addgene35512) AAV9.CamKIIa.hChR2(E123T/T159C).mCherry.WPRE.hGH(Addgene35512) AAV9.CaMKIIa.hChR2 (E123T/T159C)-eYFP.hGH (Addgene35511) MTA for original source material MTA for original source material MTA for original source material Deisseroth: Ultrafast Optogenetic Control: ChETA(E123T/H134R) AV-1-26967M AV-2-26967M AV-5-26967M AV-9-26967M AV-1-26967P AV-2-26967P AV-5-26967P AV-9-26967P AAV1.hSyn.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967M) AAV2.hSyn.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967M) AAV5.hSyn.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967M) AAV9.hSyn.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967M) AAV1.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967P) AAV2.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967P) AAV5.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967P) AAV9.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26967P) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material Deisseroth: Optical Excitation: Channelrhodopsin ChR2(H134R) AV-1-26973P AV-5-26973P AV-9-26973P AV-1-20938M AV-5-20938M AV-9-20938M AV-1-26975 AV-1-26969P AV-2-26969P AV-5-26975 AV-5-26969P AV-9-26969P AV-9-26975 AV-9-PV1522 AAV1.hSyn.ChR2(H134R)-eYFP.WPRE.hGH (Addgene26973P) AAV5.hSyn.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26973P) AAV9.hSyn.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26973P) AAV1.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (Addgene20938M) AAV5.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (Addgene20938M) AAV9.CAG.hChR2(H134R)-mCherry.WPRE.SV40 (Addgene20938M) AAV1.CaMKIIa.hChR2(H134R)-mCherry.WPRE.hGH (Addgene26975) AAV1.CamKIIa.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26969P) AAV2.CamKIIa.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26969P) AAV5.CaMKIIa.hChR2(H134R)-mCherry.WPRE.hGH (Addgene26975) AAV5.CaMKIIa.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26969P) AAV9.CamKIIa.hChR2(H134R)-eYFP.WPRE.hGH (Addgene26969P) AAV9.CaMKIIa.hChR2(H134R)-mCherry.WPRE.hGH (Addgene26975) AAV9.EF1a.ChR2-YFP.WPRE.hGH (Stanford) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material K. Deisseroth / Stanford MTA Svoboda: Optical Excitation: Channelrhodopsin ChR2 AV-1-20071P AV-5-20071P AV-9-20071P AAV1.CAG.ChR2-Venus.WPRE.SV40 (Addgene20071) AAV5.CAG.ChR2-Venus.WPRE.SV40 (Addgene20071) AAV9.CAG.ChR2-Venus.WPRE.SV40 (Addgene20071) MTA for original source material MTA for original source material MTA for original source material Deisseroth: 3rd Generation Optogenetic Inhibition: eNpHR3.0 AV-1-26972P AV-9-26972P AV-1-26971P AV-9-26971P AAV1.hSyn.eNpHR3.0-eYFP.WPRE.hGH (Addgene26972P) AAV9.hSyn.eNpHR3.0-eYFP.WPRE.hGH (Addgene26972P) AAV1.CamKIIa.eNpHR3.0-eYFP.WPRE.hGH (Addgene26971P) AAV9.CamKIIa.eNpHR3.0-eYFP.WPRE.hGH (Addgene26971P) MTA for original source material MTA for original source material MTA for original source material MTA for original source material Deisseroth: Optical Inhibition: Halorhodopsin NpHR Cre-Dependent Expression Vectors - by gene Allen Institute: Cre-dependent Control Fluorophore AV-5-ALL854 AV-2-ALL854 AV-1-ALL854 AV-1-ALL856 AV-1-ALL864 5/20/2014 AAV5.CAG.Flex.eGFP.WPRE.bGH (AllenInstitute854) AAV2.CAG.Flex.eGFP.WPRE.bGH (AllenInstitute854) AAV1.CAG.Flex.eGFP.WPRE.bGH (AllenInstitute854) AAV1.CAG.LSL.tdTomato.bGH (AllenInstitute856) AAV1.CAG.Flex.tdTomato.WPRE.bGH (AllenInstitute864) Allen Institute MTA Amendment Allen Institute MTA Amendment Allen Institute MTA Amendment Allen Institute MTA Amendment Allen Institute MTA Amendment Page 2 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements AV-1-ALL851 AV-9-ALL854 AV-9-ALL864 AV-9-ALL856 AV-9-ALL851 AAV1.CAG.LSL.eGFP.bGH(AllenInstitute851) AAV9.CAG.Flex.eGFP.WPRE.bGH (AllenInstitute854) AAV9.CAG.Flex.tdTomato.WPRE.bGH (AllenInstitute864) AAV9.CAG.LSL.tdTomato.bGH (AllenInstitute856) AAV9.CAG.LSL.eGFP.bGH (AllenInstitute851) Allen Institute MTA Amendment Allen Institute MTA Amendment Allen Institute MTA Amendment Allen Institute MTA Amendment Griesbeck: Cre-dependent Calcium Sensor: Twitch2B AV-1-49531M AAV1.CAG.Flex.Twitch2B.WPRE.SV40 (Addgene 49531M) MTA for original source material GENIE: Cre-dependent Calcium Sensor: GCaMP6 AV-1-PV2819 AV-1-PV2821 AV-1-PV2820 AV-5-PV2819 AV-5-PV2821 AV-5-PV2820 AV-9-PV2820 AV-9-PV2821 AV-9-PV2819 AV-1-PV2816 AV-1-PV2817 AV-1-PV2818 AV-5-PV2816 AV-5-PV2817 AV-5-PV2818 AV-9-PV2818 AV-9-PV2817 AV-9-PV2816 AAV1.Syn.Flex.GCaMP6f.WPRE.SV40 AAV1.Syn.Flex.GCaMP6s.WPRE.SV40 AAV1.Syn.Flex.GCaMP6m.WPRE.SV40 AAV5.Syn.Flex.GCaMP6f.WPRE.SV40 AAV5.Syn.Flex.GCaMP6s.WPRE.SV40 AAV5.Syn.Flex.GCaMP6m.WPRE.SV40 AAV9.Syn.Flex.GCaMP6m.WPRE.SV40 AAV9.Syn.Flex.GCaMP6s.WPRE.SV40 AAV9.Syn.Flex.GCaMP6f.WPRE.SV40 AAV1.CAG.Flex.GCaMP6f.WPRE.SV40 AAV1.CAG.Flex.GCaMP6m.WPRE.SV40 AAV1.CAG.Flex.GCaMP6s.WPRE.SV40 AAV5.CAG.Flex.GCaMP6f.WPRE.SV40 AAV5.CAG.Flex.GCaMP6m.WPRE.SV40 AAV5.CAG.Flex.GCaMP6s.WPRE.SV40 AAV9.CAG.Flex.GCaMP6s.WPRE.SV40 AAV9.CAG.Flex.GCaMP6m.WPRE.SV40 AAV9.CAG.Flex.GCaMP6f.WPRE.SV40 GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GCaMP6 acknowledgement GENIE: Cre-dependent Calcium Sensor: GCaMP5G AV-1-PV2540 AV-5-PV2540 AV-9-PV2540 AV-1-PV2541 AV-9-PV2541 AAV1.hSyn.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV5.hSynFlex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV9.hSyn.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV1.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 AAV9.CAG.Flex.GCaMP5G(GCaMP3-T302L.R303P.D380Y).WPRE.SV40 GCaMP5 acknowledgement GCaMP5 acknowledgement GCaMP5 acknowledgement GCaMP5 acknowledgement GCaMP5 acknowledgement Looger: Cre-dependent Calcium Sensor: GCaMP3 AV-1-PV1921 AV-5-PV1921 AV-9-PV1921 AV-9-PV1921 AAV1.hSyn.Flex.GCaMP3.WPRE.SV40 AAV5.hSyn.Flex.GCaMP3.WPRE.SV40 AAV9.hSyn.Flex.GCaMP3.WPRE.SV40 AAV9.hSyn.Flex.GCaMP3.3.SV40 GCaMP3 acknowledgement GCaMP3 acknowledgement GCaMP3 acknowledgement GCaMP3 acknowledgement Looger: Cre-dependent Glutamate Sensor: iGluSnFr AV-1-PV2724 AV-5-PV2724 AV-9-PV2724 AV-1-PV2725 AV-5-PV2725 AV-9-PV2725 AAV1.hSyn.Flex.iGluSnFr.WPRE.SV40 AAV5.hSyn.Flex.iGluSnFr.WPRE.SV40 AAV9.hSyn.Flex.iGluSnFr.WPRE.SV40 AAV1.CAG.Flex.iGluSnFr.WPRE.SV40 AAV5.CAG.Flex.iGluSnFr.WPRE.SV40 AAV9.CAG.Flex.iGluSnFr.WPRE.SV40 K. Deisseroth / Stanford MTA iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement iGluSnFr acknowledgement Cohen: Cre-dependent Fluorogenetic Voltage Sensor: ArcLightD Lentiviral Vectors For General Applications LV-G--PV1300 LV-G--PV1298 LV-G--PV1301 LV-G--PV1339 LV-G--PV1249 VSVG.HIV.SIN.cPPT.CMV.eGFP.WPRE VSVG.HIV.SIN.cPPT.CMV.ffLuciferase.WPRE VSVG.HIV.SIN.cPPT.CMV.LacZ.WPRE VSVG.HIV.SIN.cPPT.EF1a.eGFP.WPRE VSVG.HIV.SIN.cPPT.CMV.eGFP.WPRE.PGK.bla LV-G-20944P LV-G-14750P LV-G-15814P VSVG.HIV.CaMKIIa.hChR2(H134R)-eYFP.WPRE (Addgene20944) VSVG.HIV.cPPT.CamKII.Halo-GFP.WPRE (Addgene14750) VSVG.HIV.cPPT.CamKII.ChR2-GFP.WPRE (Addgene15814) For CNS Applications 5/20/2014 MTA for original source material MTA for original source material MTA for original source material Page 3 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements LV-G-26967P LV-G-PV2001 LV-G-20945P VSVG.CaMKIIa.ChETA(E123T/H134R)-eYFP.WPRE (Addgene26967P) VSVG.HIV.SIN.cPPT.CaMKII.eGFP.WPRE VSVG.HIV.SIN.Syn.ChR2(H134R)-eYFP.WPRE(Addgene20945) MTA for original source material MTA for original source material For iPSC Applications LV-G-15949P LV-G-15950P LV-G-15951P LV-G-15952P LV-G-15953P LV-G-20328P VSVG.HIV.LOVE.cPPT.CMV.GFP.WPRE (Addgene15949) VSVG.LOVE.cPPT.CMV.mKlf4.WPRE (Addgene15950) VSVG..LOVE.cPPT.CMV.mMyc.WPRE (Addgene15951) VSVG..LOVE.cPPT.CMV.mOct4.WPRE (Addgene15952) VSVG.HIV.LOVE.cPPT.CMV.mSox2.WPRE (Addgene15953) VSVG.HIV.SIN.FUW.Ubc.Oct4.P2A.Sox2.T2A.Klf4.E2A.cMyc.WPRE (Addgene20328) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material Adenoviral Vectors AD-5-PV3424 AD-5-PV3425 H5.040.CMV.GFP-DF508.CFTR H5'.040.CMV.GFP-CFTR AD-5-PV0504 AD-5-PV1028 AD-5-PV0671 AD-5-PV1096 H5'.040.CMV.eGFP H5'.040.CMV.ffluciferase H5'.040.CMV.LacZ H5'.040.CMV.PI.Cre For General Applications Pierebone: Cre-dependent Fluorogenetic Voltage Sensor: ArcLightD AV-1-36857M AAV1.hSyn.Flex.ArcLightD.WPRE.SV40(Addgene36857M) MTA for original source material Boyden: Cre-dependent Optogenetic Inhibition: Arch and ArchT AV-1-PV2432 AV-5-PV2432 AV-9-PV2432 AAV1.CBA.Flex.Arch-GFP.WPRE.SV40 (Addgene22222) AAV5.CBA.Flex.Arch-GFP.WPRE.SV40 (Addgene22222) AAV9.CBA.Flex.Arch-GFP.WPRE.SV40 (Addgene22222) E. Boyden / MIT acknowledgement E. Boyden / MIT acknowledgement E. Boyden / MIT acknowledgement Deisseroth: Cre-dependent Red-Shifted Optical Excitation C1V1(E122T/E162T) AV-9-35498M AV-9-35497 AAV9.Ef1a.DIO.C1V1(E122T/E162T).TS.mCherry.hGH(Addgene35498M) AAV9.Ef1a.DIO.C1V1 (E122T/E162T).TS.eYFP.WPRE.hGH (Addgene35497) MTA for original source material MTA for original source material Deisseroth: Cre-dependent Stablized Step Function Opsins: ChR2(C128S/D156A) AV-1-35503 AV-1-35504 AV-9-35503 AAV1.EF1a.DIO.hChR2(C128S/D156A)-eYFP.WPRE.hGH (Addgene35503) AAV1.Ef1a.DIO hChR2 (C128S/D156A).mCherry.WPRE.hGH.(Addgene35504) AAV9.EF1a.DIO.hChR2(C128S/D156A)-eYFP.WPRE.hGH(Addgene35503) MTA for original source material MTA for original source material MTA for original source material Deisseroth: Cre-dependent 2nd Generation Ultrafast Optogenetic Control: ChR2(E123A) AV-1-35507 AV-5-35506 AAV1.EF1a.DIO.ChR2 (E123A).EYFP.WPRE.hGH (Addgene35507) AAV5.CaMKII.hChR2(E123A).mCherry.WPRE.hGH.(Addgene35506) MTA for original source material MTA for original source material Deisseroth: Cre-dependent 2nd Generation Ultrafast Optogenetic Control: ChR2 (E123T/T159C) Deisseroth: Cre-dependent Ultrafast Optogenetic Control: ChETA(E123T/H134R) AV-1-26968P AV-5-26968P AV-9-26968P AAV1.Ef1a.DIO.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26968) AAV5.EF1a.DIO.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26968) AAV9.Ef1a.DIO.ChETA(E123T/H134R)-eYFP.WPRE.hGH (Addgene26968) MTA for original source material MTA for original source material MTA for original source material Deisseroth: Cre-dependent Optical Excitation: ChR2(H134R) AV-1-20297P AV-1-20298P AV-5-20297P AV-5-20298P AV-9-20297P AV-9-20298P AAV1.EF1.dflox.hChR2(H134R)-mCherry.WPRE.hGH (Addgene20297) AAV1.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH(Addgene20298) AAV5.EF1.dflox.hChR2(H134R)-mCherry.WPRE.hGH (Addgene20297) AAV5.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH(Addgene20298P) AAV9.EF1.dflox.hChR2(H134R)-mCherry.WPRE.hGH (Addgene20297) AAV9.EF1a.DIO.hChR2(H134R)-eYFP.WPRE.hGH(Addgene20298) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material Sternson: Cre-dependent Optical Excitation: ChR2, ChR2(H134R) AV-1-18916P AV-1-18917P AV-5-18917P AV-9-18916P AV-9-18917P AAV1.CBA.Flex.ChR2(H134R)-mCherry.WPRE.SV40 (Addgene18916) AAV1.CAGGS.Flex.ChR2-tdTomato.WPRE.SV40 (Addgene18917) AAV5.CAGGS.Flex.ChR2-tdTomato.WPRE.SV40 (Addgene18917) AAV9.CBA.Flex.ChR2(H134R)-mCherry.WPRE.SV40 (Addgene18916) AAV9.CAGGS.Flex.ChR2-tdTomato.WPRE.SV40 (Addgene18917) MTA for original source material MTA for original source material MTA for original source material MTA for original source material MTA for original source material Deisseroth: Cre-dependent 3rd Generation Optogenetic Inhibition: eNpHR3.0 AV-1-26966P AV-9-26966P AAV1.EF1a.DIO.eNpHR3.0-eYFP.WPRE.hGH (Addgene26966) AAV9.EF1a.DIO.eNpHR3.0-eYFP.WPRE.hGH (Addgene26966) MTA for original source material MTA for original source material Deisseroth: Cre-dependent Optical Inhibition: NpHR 5/20/2014 Page 4 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements AV-1-20949P AV-5-20949P AV-9-20949P AAV1.EF1a.DIO.eNpHR-eYFP.WPRE.hGH (Addgene20949) AAV5.EF1a.DIO.eNpHR-eYFP.WPRE.hGH (Addgene20949) AAV9.EF1a.DIO.eNpHR-eYFP.WPRE.hGH (Addgene20949) MTA for original source material MTA for original source material MTA for original source material Deisseroth: Cre-dependent Control Fluorophore AV-2-27056 AV-5-27056 AV-9-27056 AV-1-27056 AAV2.EF1a.DIO.eYFP.WPRE.hGH (Addgene27056) AAV5.EF1a.DIO.eYFP.WPRE.hGH (Addgene27056) AAV9.EF1a.DIO.eYFP.WPRE.hGH (Addgene27056) AAV1.EF1a.DIO.eYFP.WPRE.hGH (Addgene27056) MTA for original source material MTA for original source material MTA for original source material Sanes: Brainbow AAV Vectors AV-9-PV2453 AV-9-PV2454 AAV9.hEF1a.lox.TagBFP.lox.eYFP.lox.WPRE.hGH-InvBYF(Harvard) AAV9.hEF1a.lox.mCherry.lox.mTFP1.lox.WPRE.hGH-InvCheTF(Harvard) For Cre Recombinase Delivery Cre Vectors - by Gene or Gene Fusion Cre AV-1-PV2676 AV-1-PV2396 AV-9-PV2396 AV-8-PV1091 AV-1-PV1090 AV-2-PV1090 AV-5-PV1090 AV-6-PV1090 AV-8-PV1090 AV-9-PV1090 AV-10-PV1090 AAV1.hSyn.Cre.WPRE.hGH AAV1.CamKII0.4.Cre.SV40 AAV9.CamKII0.4.Cre.SV40 AAV8.TBG.PI.Cre.rBG AAV1.CMV.PI.Cre.rBG AAV2.CMV.PI.Cre.rBG AAV5.CMV.PI.Cre.rBG AAV6.2.CMV.PI.Cre.rBG AAV8.CMV.PI.Cre.rBG AAV9.CMV.PI.Cre.rBG AAVrh10.CMV.PI.Cre.rBG eGFP-Cre AV-1-PV1848 AV-9-PV1848 AV-1-PV2521 AV-9-PV2521 AV-1-PV2004 AV-2-PV2004 AV-5-PV2004 AV-8-PV2004 AV-9-PV2004 AAV1.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV9.hSyn.HI.eGFP-Cre.WPRE.SV40 AAV1.CamKII.HI.eGFP-Cre.WPRE.SV40 AAV9.CamKII.HI.eGFP-Cre.WPRE.SV40 AAV1.CMV.HI.eGFP-Cre.WPRE.SV40 AAV2.CMV.HI.eGFP-Cre.WPRE.SV40 AAV5.CMV.HI.eGFP-Cre.WPRE.SV40 AAV8.CMV.HI.eGFP-Cre.WPRE.SV40 AAV9.CMV.HI.eGFP-Cre.WPRE.SV40 For Tissue-Specific Applications Expression Vectors - by Promoter Synapsin (neuron-specific) AV-1-PV1696 AV-1-PV2642 AV-5-PV1696 AV-9-PV1696 AV-9-PV2642 AAV1.hSyn.eGFP.WPRE.bGH AAV1.hSyn.TurboRFP.WPRE.rBG AAV5.hSyn.eGFP.WPRE.bGH AAV9.hSyn.eGFP.WPRE.bGH AAV9.hSyn.TurboRFP.WPRE.rBG CamKII (excitatory glutamatergic neuron-specific) AV-1-PV1917 AV-2-PV1917 AV-5-PV1917 AV-9-PV1917 AAV1.CamKII0.4.eGFP.WPRE.rBG AAV2.CamKII0.4.eGFP.WPRE.rBG AAV5.CamKII0.4.eGFP.WPRE.rBG AAV9.CamKII0.4.eGFP.WPRE.rBG TH (tyrosine hydroxylase) GFAP (astrocyte-specific) AV-5-PV2380 AAV5.GFA104.eGFP.WPRE.bGH TBG (liver-specific) AV-8-PV1302 AV-8-PV0146 AV-8-PV0142 AV-8-PV0148 5/20/2014 AAV8.TBG.PI.ffLuciferase.rBG AAV8.TBG.PI.eGFP.WPRE.bGH AAV8.TBG.PI.LacZ.bGH AAV8.TBG.PI.Null.bGH Page 5 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name AV-9-PV0146 AAV9.TBG.PI.eGFP.WPRE.bGH Additional Requirements cTNT (heart specific) AV-9-PV1967 AAV9.cTnT.PI.eGFP.WPRE.rBG tMCK (muscle-specific) AV-9-PV1845 AAV9.tMCK.PI.eGFP.WPRE.bGH Ins (pancreas-specific) Adp (adipose-specific) For General Applications Expression Vectors - by Promoter-Gene CMV-eGFP AV-1-PV0101 AV-2-PV0101 AV-5-PV0101 AV-6-PV0101 AV-6.2-PV0101 AV-7-PV0101 AV-8-PV0101 AV-9-PV0101 AV-10-PV0101 AAV1.CMV.PI.eGFP.WPRE.bGH AAV2.CMV.PI.eGFP.WPRE.bGH AAV5.CMV.PI.eGFP.WPRE.bGH AAV6.CMV.PI.eGFP.WPRE.bGH AAV6.2.CMV.PI.eGFP.WPRE.bGH AAV7.CMV.PI.eGFP.WPRE.bGH AAV8.CMV.PI.eGFP.WPRE.bGH AAV9.CMV.PI.eGFP.WPRE.bGH AAVrh10.CMV.PI.eGFP.WPRE.bGH CMV-TurboRFP AV-1-PV2177 AV-5-PV2177 AV-8-PV2177 AV-9-PV2177 AAV1.CMV.TurboRFP.WPRE.rBG AAV5.CMV.TurboRFP.WPRE.rBG AAV8.CMV.TurboRFP.WPRE.rBG AAV9.CMV.TurboRFP.WPRE.rBG CMV-Luciferase AV-1-PV0105 AV-2-PV0105 AV-5-PV0105 AV-8-PV0105 AV-8-PV0105 AV-9-PV0105 AAV1.CMV.ffLuciferase.SV40 AAV2.CMV.ffLuciferase.SV40 AAV5.CMV.ffLuciferase.SV40 AAV8.CMV.ffLuciferase.SV40 AAV9.CMV.ffLuciferase.SV40 CMV-LacZ AV-1-PV0102 AV-2-PV0102 AV-5-PV0102 AV-6-PV0102 AV-7-PV0102 AV-8-PV0102 AV-9-PV0102 AAV1.CMV.LacZ.bGH AAV2.CMV.LacZ.bGH AAV5.CMV.LacZ.bGH AAV6.CMV.LacZ.bGH AAV7.CMV.LacZ.bGH AAV8.CMV.LacZ.bGH AAV9.CMV.LacZ.bGH CAG(CB7)-eGFP AV-2-PV1963 AV-1-PV1046 AV-1-PV1963 AV-2-PV1046 AV-5-PV1046 AV-5-PV1963 AV-7-PV1046 AV-8-PV1046 AV-8-PV1963 AV-9-PV1046 AV-9-PV1963 AV-10-PV1963 AV-10-PV1046 AAV2.CB7.CI.eGFP.WPRE.rBG AAV1.CB7.CI.eGFP.rBG AAV1.CB7.CI.eGFP.WPRE.rBG AAV2.CB7.CI.eGFP.rBG AAV5.CB7.CI.eGFP.rBG AAV5.CB7.CI.eGFP.WPRE.rBG AAV7.CB7.CI.eGFP.rBG AAV8.CB7.CI.eGFP.rBG AAV8.CB7.CI.eGFP.WPRE.rBG AAV9.CB7.CI.eGFP.rBG AAV9.CB7.CI.eGFP.WPRE.rBG AAVrh10.CB7.CI.eGFP.WPRE.rBG AAVrh10.CB7.CI.eGFP.rBG CAG(CB7)-mCherry AV-1-PV1969 AV-9-PV1969 5/20/2014 AAV1.CB7.CI.mCherry.WPRE.rBG AAV9.CB7.CI.mCherry.WPRE.rBG Page 6 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name Additional Requirements CAG(CB7)-mCerulean AV-1-PV1970 AV-9-PV1970 AAV1.CB7.CI.mCerulean.WPRE.rBG AAV9.CB7.CI.mCerulean.WPRE.rBG CAG(CB7)-TurboRFP AV-1-PV2055 AV-9-PV2055 AAV1.CB7.CI.TurboRFP.WPRE.rBG AAV9.CB7.CI.TurboRFP.WPRE.rBG CAG(CB7)-tdTomato AV-1-PV2126 AAV1.CAG.tdtomato.WPRE.SV40 EF1a-eGFP AV-5-PV2131 AV-9-PV2131 AAV5.EF1a.eGFP.WPRE.rBG AAV9.EF1a.eGFP.WPRE.rBG For Gene Silencing AV-9-PV0111 AAV9.U6.shRLuc.CMV.ZsGreen.SV40 For Bicistronic Gene Expression AV-9-PV1262 AV-9-PV1263 AV-9-PV1265 AV-9-PV1429 AV-9-PV1814 AV-9-PV1960 AAV9.CMV.eGFP.T2A.ffLuciferase.SV40 AAV9.TBG.PI.eGFP.T2A.ffLuciferase.rBG AAV9.TBG.ffLuciferase.T2A.hA1AT.rBG AAV9.cTNT.PI.eGFP.T2A.ffLuciferase.rBG AAV9.tMCK.PI.eGFP.T2A.ffLuciferase.SV40 AAV9.tMCK.eGFP.T2A.ffLuciferase.WPRE.SV40 For Regulated Expression Plasmids AAV Plasmids AAV AD HELPER PLASMID PL-F-PVADF6 pAd DeltaF6 AAV REP CAP PLASMIDS (TRANS PLASMIDS) PL-T-PV0001 PL-T-PV0002 PL-T-PV0003 PL-T-PV0004 PL-T-PV0005 PL-T-PV0006 PL-T-PV0007 PL-T-PV0008 PL-T-PV0009 PT-T-PV0016 pAAV2/1 pAAV2/2 pAAV2/5 pAAV2/6 pAAV2/6.2 pAAV2/7 pAAV2/8 pAAV2/9 pAAV2/rh10 pAAV2/hu11 AAV EXPRESSION PLASMIDS FOR INSERTION (CIS PLASMIDS) PL-C-PV0100 PL-C-PV2057 PL-C-PV1044 PL-C-PV1015 PL-C-PV1045 pZac2.1(pAAV.CMV.PI.SV40) pENN.AAV.CMVs.PI.WPRE.RBG pENN.AAV.CB7.CI.rBG pENN.AAV.TBG.PI.rBG pENN AAV CB6 PI MASTER AAV EXPRESSION PLASMIDS FOR REPLACEMENT (CIS PLASMIDS) PL-C-PV2396 PL-C-PV2676 PL-C-PV1969 PL-C-PV1888 PL-C-PV2380 PL-C-PV2004 PL-C-PV0101 PL-C-PV2004 PL-C-PV0142 PL-C-PV1091 PL-C-PV1091 PL-C-PV1848 PL-C-PV0146 5/20/2014 pENN.AAV.CamKII 0.4.Cre.SV40 (p2396) pENN.AAV.hSynapsin.Cre.WPRE.hGH (p2676) pENN.AAV.CB7.CI.mCherry.WPRE.RBG (p1969) pZac2.1-luc-IRES-eGFP.SV40 (P1888-R) pAAV.GFA104.PI.eGFP.WPRE.bGH (p2380) pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 pAAV.CMV.PI.EGFP.WPRE.bGH (pZac2.1.EGFP3) MASTER pAAV.CMV.HI.eGFP-Cre.WPRE.SV40 (P2004-2) pAAV.TBG.PI.LacZ MASTER pENN AAV TBG PI.Cre.rBG MASTER pENN AAV TBG PI.Cre.rBG pENN.AAV.hSyn.HI.eGFP-Cre.WPRE.SV40 pAAV.TBG.PI.eGFP.WPRE.bGH(p0146) MASTER Page 7 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Catalog Number Vector Name PL-C-PV1046 PL-C-PV1963 PL-C-PV1014 PL-C-PV1372 PL-C-PV1967 PL-C-PV1696 PL-C-PV1474 PL-C-PV1917 PL-C-PV1646 PL-C-PV1060 pENN.AAV.CB7.CI.eGFP.rBG(p1046) pENN.AAV.CB7.CI.eGFP.WPRE.rBG pENN.AAV.TBG.PI.eGFP.rBG(p1014-R) pENN.AAV.cTnT.PI.eGFP.RBG (p1372-2) pENN.AAV.cTNT.PI.eGFP.WPRE.rBG pAAV.hSynap.eGFP.WPRE.bGH (p1696) pENN.AAV.CaMKII0.4.eGFP.rBG pENN.AAV.CamKII0.4.eGFP.WPRE.rBG pENN.AAV.TH.eGFP.rBG pENN.AAV.CMVs.PI.eGFP.rBG Additional Requirements AAV SIRNA PLASMIDS FOR CLONING PL-C-PV0111 PL-C-PV1867 PL-C-PV1781 PL-C-PV1782 pAAV.U6.shRLuc.CMV.ZsGreen1.SV40 (p0111) pENN.AAV.U6.ShRLuc.CMV.eGFP.SV40 pAAV.H1.shRLuc.CMV.ZsGreen.SV40 pAAV.H1.shRLuc.CMV.TurboRFP.SV40 GENIE: AAV GCAMP6 PLASMIDS PL-C-PV3081 PL-C-PV3080 pAAV.CAG.GCaMP6f.WPRE.SV40 (P3081) pAAV.CAG.GCaMP6m.WPRE.SV40 (P3080) Lentiviral Plasmids PL-L-PV1300 PL-L-PV1249 LV-G--PV1621 PL-L-PV1324 pHIV.SIN.cPPT.CMV.EGFP.WPRE (p1300) pENN.HIV.SIN.cPPT.CMV.EGFP.WP.PGK.bla (p1249) pENN.HIV/SIN.cPPT.CMV.nLacZ.WPRE pLenti6.2-GW.SIN.cPPT.WP Adenoviral & Shuttle Plasmids 5/20/2014 Page 8 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 Additional Requirements Detail GENIE acknowledgement Janelia Farm has generously allowed this material to be distributed with the understanding that requesting investigators need to acknowledge the Janelia Farm program in any publication in which the material was used, specifically Loren L. Looger, Ph.D., Jasper Akerboom, Ph.D., Douglas S. Kim, Ph.D. and the Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project at Janelia Farm Research Campus Howard Hughes Medical Institute GCaMP3 acknowledgement Loren L. Looger, Ph.D. and the Janelia Farm Research Campus of the Howard Hughes Medical Institute has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. iGluSnFr acknowledgement Loren L. Looger, Ph.D. and the Janelia Farm Research Campus of the Howard Hughes Medical Institute has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. GCaMP5 acknowledgement The Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Farm Research Campus of the Howard Hughes Medical Institute has generously allowed this material to be distributed with the understanding that requesting investigators need to acknowledge the GENIE Program and the Janelia Farm Research Campus in any publication in which the material was used, specifically Loren L. Looger, Ph.D., Jasper Akerboom, Ph.D., Douglas S. Kim, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute. GCaMP6 acknowledgement The Genetically-Encoded Neuronal Indicator and Effector (GENIE) Project and the Janelia Farm Research Campus of the Howard Hughes Medical Institute has generously allowed this material to be distributed with the understanding that requesting investigators need to acknowledge the GENIE Program and the Janelia Farm Research Campus in any publication in which the material was used, specifically Vivek Jayaraman, Ph.D., Rex A. Kerr, Ph.D., Douglas S. Kim, Ph.D., Loren L. Looger, Ph.D., Karel Svoboda, Ph.D. from the GENIE Project, Janelia Farm Research Campus, Howard Hughes Medical Institute. L. Cohen / KIST acknowledgement Doug Storace, Uhna Sung, Larry Cohen and the Korea Institute of Science and Technology have provided this material for distribution to the research community and would be grateful for an acknowledgement in any publication resulting from its use L. Looger/HHMI acknowledgement Loren L. Looger, Ph.D. and Janelia Farm Research Campus of the Howard Hughes Medical Institute has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material MTA for original source material An MTA for the original source material is required. An MTA can be obtained through the process of ordering the original source material through Addgene at www.addgene.org or can be obtained directly from the original providing laboratory / institution. Confirmation of an MTA must be provided to the Vector Core prior to release of the material. 5/20/2014 Page 9 of 10 Penn Vector Core Gene Therapy Program Department of Pathology and Laboratory Medicine Division of Transfusion Medicine TRL Suite 2000, 125 S 31st Street | Philadelphia PA 19104-3403 | Tel: 215-573-0633 |Fax: 215-261-2442 K. Deisseroth / Stanford MTA An MTA for the original source material is required. The Deisseroth Lab policy for sharing materials starts with an email request from the PI of the lab addressed to Dr. Karl Deisseroth (and copied to Charu Ramakrishnan). The details for requesting MTAs for Deisseroth lab materials can be found at http://www.stanford.edu/group/dlab/optogenetics/request_dna.html. Confirmation of the MTA must be provided to the Vector Core prior to release of the material. P. Haydon / Tufts MTA An MTA or "Permission for Use" for the original source material is required. An MTA or Permission can be obtained directly from the Haydon laboratory. Confirmation of an MTA / Permission must be provided to the Vector Core prior to release of the material. E. Boyden / MIT acknowledgement Ed Boyden, Ph.D. and the Massachusetts Institute of Technology has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material Allen Institute MTA Amendment An MTA Amendment for the original source material is required (follow the link to locate the appropriate amendment form) R. Campbell / U.Alberta acknowledgement Robert Campbell, Ph.D. and the University of Alberta, Canada, has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. Dr. Campbell may be contacted directly for the use of vectors based on additional materials generated in his laboratory. L. Lagnado/Cambridge acknowledgement Leon Lagnado, Ph.D. and Cambridge University has kindly provided this material for distribution to the research community and should be acknowledged in any publication resulting from the use of the material. 5/20/2014 Page 10 of 10
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