Cellular and Structural Neuroscience Markers SMI® Monoclonals and β-Tubulin Antibodies BioLegend is proud to offer SMI® monoclonal antibodies in our class of neuroscience antibody research tools. Originally developed by Dr. Ludwig Sternberger, these antibodies have become the gold standard as neural and glial markers and have an extensive publication history in scientific literature. In addition to our β-Tubulin antibodies, the SMI® product line offers researchers high quality reagents to support investigations into neurodegenerative diseases such as Alzheimer’s disease and Parkinson’s disease. Key Features: • Antibodies specific for phosphorylated and nonphosphorlylated neurofilaments, myelin basic proteins, and GFAP. • Excellent selection of specificities for oligodendrocytes, astrocytes, and other glial cells. • Each high quality antibody is quality tested for specific, robust, and consistent performance. • Experienced technical support staff and in-house generated evaluation data. Rat hippocampus stained with anti-GFAP (red) and anti-NF-H (PCK592P, green). Photo courtesy of Molecular Expressions. Rat cortical neurons and glia in mixed tissue culture stained with antiNF-H (red) and GFAP (green, PRB-571C). The nuclei are stained with Hoescht dye (blue). Photo courtesy of EnCor Biotechnology Inc. To explore the full line of products, visit: antibody.biolegend.com Human brain section stained with anti-GFAP (brown, SMI-21R), counterstained with Hematoxylin (blue). Toll-Free Tel (US & Canada): 1.800.223.0796 Tel:1.781.915.5200 Email: [email protected] antibody.biolegend.com 07-0092-00 World-Class Quality | Superior Customer Support | Outstanding Value Cellular and Structural Neuroscience Markers Phosphorylated Neurofilaments Non-Phosphorylated Neurofilaments Specificity Clone Reactivity Application(s) Cat. No. Specificity Clone Reactivity Application(s) Cat. No. Neurofilament H, Phosphorylated SMI 310 Mammal WB, ICC, ELISA SMI-310R Neurofilament H, Non-Phosphorylated Polyclonal Hu, Rat, Ms, Cat, Ckn WB, IHC PCK-592P Neurofilament H, Phosphorylated SMI 34 Mammal WB, ICC, IHC, ELISA SMI-34R Neurofilament H, Non-Phosphorylated SMI 32 Mammal WB, ICC, IHC, ELISA SMI-32R Neurofilament H, Phosphorylated SMI 36 Mammal WB, ICC, IHC, ELISA SMI-36R Neurofilament H, Non-Phosphorylated SMI 37 Mammal WB, ICC, IHC, ELISA SMI-37R Neurofilaments, Hypophosphorylated SMI 35 Mammal WB, ICC, IHC, ELISA SMI-35R Neurofilament H, Non-Phosphorylated SMI 38 Mammal WB, ICC, IHC, ELISA SMI-38R Neurofilaments, Phosphorylated SMI 31 Mammalian, Ckn, Xenopus WB, IHC, ELISA SMI-31R Neurofilament H, Non-Phosphorylated SMI 39 Mammal WB, ICC, IHC, ELISA SMI-39R Neurofilaments, Phosphorylated, Purified SMI 31 Mammalian, Ckn, Xenopus WB, IHC, ELISA SMI-31P SMI 32 Mammal WB, IHC, ELISA SMI-32P Pan-Axonal Neurofilament Marker SMI 312 Mammal WB, IHC, ELISA SMI-312R Neurofilament H, Non-Phosphorylated, Purified Neurofilaments H & M, NonPhosphorylated SMI 33 Mammalian, Xenopus WB, ICC, IHC, ELISA SMI-33R Pan-Neuronal Neurofilament Marker SMI 311 Mammal WB, IHC, ELISA SMI-311R Specificity Clone Reactivity Application(s) Cat. No. GFAP Polyclonal Hu, Rat, Ms, Cat WB, ICC, IHC PRB-571C GFAP SMI 21 Hu, Dog, Primate WB, IHC, ELISA SMI-21R GFAP SMI 22 Hu, Shp, Cow, Dog, Pig, Rat, Guinea Pig, Ms, Ckn WB, ICC, IHC, ELISA SMI-22R GFAP SMI 23 Hu WB, ICC, IHC, ELISA SMI-23R GFAP SMI 24 Hu WB, ICC, IHC, ELISA SMI-24R GFAP SMI 25 Hu WB, ICC, IHC, ELISA SMI-25R GFAP SMI 26 Hu, Shp, Cow, Dog, Pig, Rat, Guinea Pig, Ms, Ckn WB, ICC, IHC, ELISA SMI-26R Oligodendrocytes Specificity Clone Reactivity Application(s) Cat. No. Myelin Basic Protein SMI 94 Mammal WB, ICC, IHC, ELISA SMI-94R Myelin Basic Protein SMI 99 Mammal WB, ICC, IHC, ELISA SMI-99P Myelin CNPase SMI 91 Hu, Rat, Ms, Shp, Dog, Pig, Cow, Primate WB, ICC, IHC, ELISA SMI-91R β-Tubulin Specificity Clone Reactivity Application(s) Cat. No. Depolymerized β-Tubulin SMI 61 Mammal WB, ICC, IHC, ELISA SMI-61R Neuronal Class III β-Tubulin TUJ1 Mammal WB, IP, ICC, IHC MMS-435P Neuronal Class III β-Tubulin Polyclonal Mammal WB, ICC, IHC PRB-435P Neuronal Class III β-Tubulin (Rabbit) TUJ1 1-15-79 Mammal WB, IP, ICC, IHC MRB-435P Neuronal Class III β-Tubulin Peptide Peptide Peptide detected by TUJ1 Antibodies WB or IP (Competition) PEP-435P Neuronal Class III β-Tubulin, Alexa Fluor® 488 Labeled TUJ1 Mammal WB, IP, ICC, IHC A488-435L Polymerized β-Tubulin SMI 62 Mammal, Green Algae, Molds WB, ICC, ELISA Astrocytes Other Specificities SMI-62R Specificity Clone Reactivity Application(s) Cat. No. GonadotropinReleasing Hormone (LHRH) SMI 41 Hu ICC, RIA SMI-41R MAP2 SMI 52 Mammal, Xenopus WB, ICC, IHC, ELISA SMI-52R MicrotubuleAssociated Protein Tau SMI 51 Hu, Cow WB, ICC, IHC, ELISA SMI-51R Ckn = Chicken ICC = Immunocytochemistry Hmr = Hamster IHC = Immunohistochemistry Rat Blood-Brain Barrier SMI 71 Rat ICC, IHC, ELISA SMI-71R Hu = Human IP = Immunoprecipitation SNAP-25 SMI 81 Mammal WB, ICC, IHC, ELISA SMI-81R M = Mouse WB = Western Blot/Immunoblot Shp = Sheep Cat. No. suffix “R” = Ascites Cat. No. suffix “P” = Purified Alexa Fluor® is a registered trademark of Life Technologies. Toll-Free Tel (US & Canada): 1.800.223.0796 Tel:1.781.915.5200 Email: [email protected] antibody.biolegend.com 07-0092-00 World-Class Quality | Superior Customer Support | Outstanding Value
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