ESM Table 1. qPCR Primer sequences. Gene Forward primer (5’to3’) Reverse primer (5’to3’) Ppargc1a ACC ATG ACT ACT GTC AGT CAC TC GTC ACA GGA GGC ATC TTT GAA G Cox5b CAG CTT GTA ATG GGT TCC ACA GT GCT GCA TCT GTG AAG AGG ACA AC Ndufv2 CAA GCC GCT GCT GTG CTT AGC CAT CCA TTC TGC CTT TG mt-Cytb ATT CCT TCA TGT CGG ACG AG AGA AGC CCC CTC AAA TTC AT AGG CCC TTT GCC AAG CTT TTC TCC TTA GAT GCA GAG TAC A Myoglobin ATC CAG CCT CTA GCC CAA TC AAA GTC CCC GGA ATG TCT CT Creb1 AAT GGT ACG GAT GGG GTA CA TCC ATC AGT GGT CTG TGC AT Glut4 ACG GTC TTC ACG TTG GTC TC CTC AAA GAA GGC CAC AAA GC CAMK4 AGG TTG TGC CTA TGG ACC TG CCA CCA GGG GGA GAT AAA Cd36 ATT GGT CAA GCC AGC T TGT AGG CTC ATC CAC TAC Dgat1 GTT CCG CCT CTG GGC ATT GAA TCG GCC CAC AAT CCA Fndc5 GGA CTC TTG GAA AAC ACC ACT G TCC ACA CAG ATG ATC TCA CCA C GTG GCA GGT AGA GCA GGA AG CCA CCT GAA AGG CAC TCT GT GAG GCC AAG AAG AGT TCT GGA T TGC CCA GGT AAG AGC TTC AA CAG CAC GGT GAA GCC ATT C GCG TGC ATC CGC TTG TG Fgf21 CTG GGG GTC TAC CAA GCA TA CAC CCA GGA TTT GAA TGA CC Vegfb TAT CTC CCA GAG CTG CCA TCT A AGC CAG AAG ATG CTC ACT TGA C Atp5b Il6 Il15 Prdm16 Peroxisome proliferator-activated receptor gamma coactivator-1 (Ppargc1a), cytochrome c oxidase subunit Vb (Cox5b), NADH dehydrogenase (ubiquinone) flavoprotein 2 (Ndufv2), cytochrome b, mitochondrial (mt-Cytb), ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit (Atp5b), cAMP response element-binding protein 1(Creb1), glucose transporter 4 (Glut4), human Ca2+/calmodulin-dependent kinase IV (CAMK4), diacylglycerol Oacyltransferase 1 (Dgat1), fibronectin type III domain containing 5 (Fndc5), interleukin 6 (Il6), interleukin 15 (Il15) PR domain containing 16 (Prdm16), fibroblast growth factor 21 (Fgf21), vascular endothelial growth factor B (Vegfb).
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