Journal of Experimental Botany
Sixteen cytosolic glutamine synthetase genes
identified in Brassica napus L. genome are
differentially regulated depending on nitrogen
regimes and leaf senescence
Mathilde Orsel, Michaël Moison, Vanessa Clouet, Justine Thomas, Françoise
Leprince, Anne-Sophie Canoy, Jérémy Just, Boulos Chalhoub, Céline MasclauxDaubresse.
5 Supplementary Tables
5 Supplementary Figures
5 supplementary Data Files
Supplementary Table S1: Clones from Genoplante and ADIS-MPIZ oilseedrape cDNA libraries used for BnaGLN1 mRNA sequencing.
A. thaliana
Gene
AtGLN1.1
AtGLN1.2
AtGLN1.3
AtGLN1.4
B. napus
Gene
BnaA.GLN1.1.a
BnaC.GLN1.1.a
BnaA.GLN1.2.a
BnaC.GLN1.2.a
BnaC.GLN1.3.a
BnaA.GLN1.3.a
BnaA.GLN1.3.c
BnaA.GLN1.4.a
BnaC.GLN1.4.b
BnaA.GLN1.4.b
AtGLN1.5
a
b
BnaC.GLN1.4.a
BnaC.GLN1.5.a
Accession (mRNA name)
X82997 (BnGSR2.2)
b
Y12460 (BnGSR2.2)
X76736 (BnGSR1.1)
Y12459 (BnGSR1.2)
JX306690
JX306693
JX306694
a
JX306696
JX306697
a
JX306692
b
JX306698
b
JX306699
b
JX306700
b
JX306701
JX306695
b
JX306691
Clone
Library (NCBI ID)
MPIZp1022G0813Q
BN20.052B22
BN40.043K19
ADIS-MPIZ 021 (Lib.16807)
Genoplante BN20 (Lib.13978)
Genoplante BN40 (Lib.13980)
MPIZp1022C0924Q
ADIS-MPIZ 021 (Lib.16807)
BN15.001A07
MPIZp1022A1123Q
Genoplante BN15 (Lib.13977)
ADIS-MPIZ 021 (Lib.16807)
Genotype
Arabella
Arabella
Arabella
Arabella
Express 617
JetNeuf
JetNeuf
Express
Tenor
Express 617
Express
Tenor
Express
Tenor
JetNeuf
Express 617
cDNA clone with SNP insertion disrupting the ORF when compared to A. thaliana cds reference sequence and BnaGLN1 contig.
Incomplete cds when compared to A. thaliana cds reference sequence.
Supplementary Table S2: Primers used for cloning and genetic mapping of BnaGLN1 gene.
A. thaliana
B. napus
Gene
Gene
AtGLN1.1
BnaA.GLN1.1.a
BnaC.GLN1.1.a
BnaA.GLN1.2.a
BnaC.GLN1.2.a
BnaC.GLN1.2.a
BnaC.GLN1.3.a
AtGLN1.2
AtGLN1.3
AtGLN1.4
Forward primer sequence
Reverse primer sequence
Usage
BnGS1.81 U / L
BnGS1.1 U / L
TCTTGGTTGGCCTATTGGC
TGGTATCTTTTGACCCAAAACCT
GAAGTGTGGATTGCTCGTTTT
CTACAAGGCTTGTTTGTATGCC
ATGAAGTGTGGATTGCTCGTTAC
GTGACATCGTGGATGCG
GCGTATAAGCAGGCCTTGTAG
CGGATTGATGCTCCACGA
ACCCCATTTGAAAGTGTTGATA
CGAGCAATCCACACTTCATC
GTGTGAGCACCAGCTCCA
CAAGAGAACGTGTTGATGTCC
BnaA.GLN1.3.a
BnGS1.2 U / L
GGGAGCTGACAAGGCAA
GCCTTCTTTCTCAGTGTCTCTC
BnaC.GLN1.3.b
BnaA.GLN1.3.b
BnaA.GLN1.3.c
BnGS1.31 UP2 / RP2
BnGS1.32 U / L
BnGS1.41 U / L
AGCTTCGACCCAAAACC
TTGAAGGGATTAGTGCC
ACGTTGCCAAGGAGGTG
GCTTTCTTTATCACCGCTAGT
TACTGTAGTTGCAGTGCG
CGGAACTGGTTTAGGGTCG
BnaC.GLN1.3.c
BnGS1.42 U / L
TTGCCAAAGAAGTGCCTT
AGGGAAGCCACCAACAG
BnaA.GLN1.4.a
BnaC.GLN1.4.a
BnGS1.61 5U / 5L
BnGS1.7 2U / 2L
BnGS1.7 4U / 5L
BnGS1.64 5U / 5L
BnGS1.64 2U / 2L
BnGS1.63 4U / 5L
BnGS1.63 2U / 3L
BnGS1.51 U / L
BnGS1.52 U / L
TTCAGATTCCTAGAAAAATGTCG
TGTCGGCTCTTGCAGATTTAAT
CTCCCGGCGATGATAGTG
ATCCCGGCAAACAAAAGG
TCTCGATCTCTCCGATTCCA
GTGAACCGATTCCGACGAAC
GATCAATCTCGATCTCTCCGATTA
AGGGAAGCCACCAACAG
CAGAAAAAATGTCTCCACTCTCC
TCAAGGTTTCTTCCTTCATGG
ATCATCGCCGGGAGCTTGC
TTCCTCATTCATGGTTTCCAG
TTTTGTTTGCCGGGATCG
CCGGCCATTTAATATCCTTTT
TTCTTCATTCATGGCTTCCA
CCTACCGGCCACTTAATATCC
TCATGCTTCCCAGTGAGG
CCATCCACAATGTCACGC
Panel , add
a
b
Panel , add
a
Panel
a
b
Panel , add
c
SD
a
b
Panel , add
d
a
DS , Panel ,
b
add
a
b
Panel , add
a
Panel
d
a
DS , Panel
c
a
SD ,Panel ,
b
add
a
Panel , cloning
a
Panel , cloning
Cloning
a
Panel , cloning
Cloning
a
Panel
Cloning
a
Panel
e
a
DY , Panel
BnaA.GLN1.4.b
BnaC.GLN1.4.b
AtGLN1.5
a
Primer couple
F / R primer name
BnGSR2.1 U / L
BnGS1.9 U / L
BnGS1.82 U / L
BnaC.GLN1.5.a
BnaA.GLN1.5.a
BnGSR1-2_Y12459_cDNA-M1-F / R
Panel of B. napus, B. oleracea and B. rapa genotypes.
Monnosomic and polysomic addition lines obtained from a cross Darmor-bzh x C1.3.
c
Mapping population Stellar x Drakar.
d
Mapping population Darmor x Smouraï.
e
Mapping population Darmor-bzh x Yudal.
b
a
b
Supplementary Table S3: Specific primers used for sequencing BnaGLN1 cDNAs.
A. thaliana
Gene
AtGLN1.3
AtGLN1.4
B. napus
Gene
BnaC.GLN1.3.a
Accession
JX306690
BnaA.GLN1.3.a
JX306693
BnaA.GLN1.3.c
JX306694
BnaA.GLN1.4.a
JX306692
BnaC.GLN1.4.a
JX306696
JX306697
JX306695
BnaC.GLN1.4.b
BnaA.GLN1.4.b
AtGLN1.5
BnaC.GLN1.5.a
JX306698
JX306699
JX306700
JX306701
JX306691
Primer name
GS1-1-13G8-1
GS1.1-rev-1
GS1.1-rev-2
BnGS_ContigEST2_1
GS1.2-52B22-1
GS1.2_EST_1
GS1.2-rev-1
GS1.2-rev-2
BnGS_ContigEST4_1
GS1.41_EST_1
GS1-41-rev-1
GS1-41-rev-2
BnGS_ContigEST6_1
GS1-61-24C9-1
GS1.61-rev-1
GS1.61-rev-2
GS1-61-6U
GS1-61-3L
BnGS_ContigEST7_1
GS1.7_EST_1
GS1-7-rev-1
GS1-7-rev-2
GS1-63-2U
GS1-63-L
GS1-64-2U
GS1-64-2L
GS1-51-23A11-1
GS1.51-rev-1
GS1.51-rev-2
Primer sequence
CGATGAGGAACGACGGAG
GAAACTAGGAAGAAAAAAAA
CCAGTTCACACCCTTTTG
GCTAGATACCTTCTCGAGAG
CGGTGAGAGTGGGGAGAGAC
TCAGCTTCGACCCTAAACCA
TTATTGAAACCGAAATAGAC
TTGTGCCTCTTGTTGGTC
AACCAGTTCCGGGTGATTGG
GTGATTGGAACGGAGCTGGA
CATCATGAAAATCCTTTATTT
CGTTAGGATGGCTAAAGA
GTAGATGCTCATTACAAAGC
TGGAAGACACGAGACTGCTG
TTATTTCTTGGAAAGAGTTT
GTAGTATGGTCCCTGAGGA
CACCAAGTCGATGAGGGAAG
CTTCCCTCATCGACTTGGTG
GTTGTTTTATCTCTTGACCC
CGATGAGAGAAGATGGAGGG
GACCGAGACTTGAATCTTGT
ATACCATGTTTCTTCGGC
GATCAATCTCGATCTCTCCGATTA
CCAACCTACCGGCCATTTA
TCTCGATCTCTCCGATTCCA
CCGGCCATTTAATATCCTTTT
GGAGCATCGGTGAGAGTGG
CAAACTGAAATTAAACCG
ACCAGGAAAGCCACCAAG
Supplementary Table S4: qPCR Primers used for BnaGLN1 gene expression analysis.
A. thaliana
B. napus
Reference
gene
Target
gene(s)
At3g01150 (AtPTB)
At1g13320 (AtPP2A)
At1g07920 (AtEF1α)
At2g28390 (AtSAND)
At5g25760 (AtUBC21)
At5g37600 (AtGLN1.1)
BnaX.PTB.a
BnaX.PP2A.a
BnaX.EF1.a
BnaX.SAND.a
BnaX.UBC21.a
BnaA.GLN1.1.a
BnaC.GLN1.1.a
BnaA.GLN1.2.a
BnaC.GLN1.2.a
BnaC.GLN1.3.a
BnaA.GLN1.3.a
BnaC.GLN1.3.b
BnaA.GLN1.3.b
Bna.GLN1.3.c a
BnaA.GLN1.4.a
BnaC.GLN1.4.a
BnaC.GLN1.4.b
BnaA.GLN1.4.b
Bna.GLN1.5.a a
BnGSL1
BnGSL2
At1g66200 (AtGLN1.2)
At3g17820 (AtGLN1.3)
At5g16570 (AtGLN1.4)
At1g48470 (AtGLN1.5)
At5g35630 (AtGLN2)
a
Reference
sequence or
accession
EV058089
EE464526
CD827228
EV384210
EE493452
BnaGLN1.1_C1
BnaGLN1.1_C2
BnaGLN1.2_C1
BnaGLN1.2_C2
BnaGLN1.3_C1
BnaGLN1.3_C2
BnaGLN1.3_C3
BnaGLN1.3_C4
BnaGLN1.3_C5
BnaGLN1.4_C1
BnaGLN1.4_C2
BnaGLN1.4_C3
BnaGLN1.4_C4
BnaGLN1.5_C1
X72751
Y12458
Primer names forward / reverse
Forward primer sequence
Reverse primer sequence
BnPTB-UP / RP
BnPP2A-UP1 /RP1
Q-BnEF1-1-UP6 /RP6
BnSAND-UP / RP
UBC21-UP1 /RP1
BnGSR2-1_X82997_cDNA-M1-F / R
BnGSR2-2_Y12460_cDNA-M2-F / R
BnGSR1-1_X76736_cDNA-M1-F / R
BnGSR1-2_Y12459_cDNA-M1-F / R
BnGS_contigEST_1-M3-F / R
BnGS_contigEST_2-M1-F / R
BnGS1_31_UP2 / RP2
BnGS1_32_UP2 / RP2
BnGS_contigEST_4-M2-F / R
BnGS_contigEST_6-M1-F / R
BnGS_contigEST_7-M2-F / R
BnGS1_63_UP2 / RP2
BnGS1_64_UP2 / RP2
BnGS_contigEST_5-M2-F / R
BnGSL1_X72751_cDNA-M3-F / R
BnGSL2_Y12458_cDNA-M1-F / R
AGCGAAAGAAGCATTGGAAG
GCTTCAATCCCTCAATCCCTCATTCCAA
CGGTGTCATCAAGAGCGTTG
CTGCTATGCTGGGTCACTCC
GTCCTCTCAACTGCGACTCA
TCTTGGTTGGCCTATTGGC
CCTGTTGGTTGGCCTATTG
AAAGATGTGAATTGGCCTGTC
CTACAAGGCTTGTTTGTATGCC
GTGACATCGTGGATGCG
GAGCGATCCATCAAAGCTG
AGCTTCGACCCAAAACC
CTGTTGAAGGGATTAGTGCC
ATGCATACACACCGGCC
GAACCGATCCCAACCAAC
GCTGGAATCAATGTCAGTGG
GACATAGTAGATTCTCATTACAAAGCC
AGCTGGGAAAGGGTACTTC
TCGATCATCCCAATGTGAAG
GGAGCGTATCCTGGTCCC
ATTCCAAGTTGGCCCGA
CAGGAGTAGGCTCAGATCAGG
ATCCTCGCTTAGCTCCACAA
GGACTTTGATTCATCTGCGGAT
TGATTGCATATCTTTATCGCCATC
GTGTGTACATGCGTGCCATT
GCGTATAAGCAGGCCTTGTAG
TCCATTGATGCCACTAATGG
GCATCAACAATGTCTCTTCCA
CGAGCAATCCACACTTCATC
AACAGGACCGACTTGGAAC
TGAACGGATCACGGAATATC
GCTTTCTTTATCACCGCTAGT
GGACTGGTTTTGGGTCG
TCTTGCTCAATCCCATACCA
GGCCACTTAGTATCCTTTTGG
CGACCCAGACCTGATCG
CGAGCGACCCAGACTTG
ATGGCTTCCAGAGGATTGT
CAATAGTACGGTCCCTGAGG
GTCCTGGCATAACTTCACCA
AACCAGCACCGTTCCAGT
Primers are not specific to one target gene but towards both BnaA.GLN1 and BnaC.GLN1 genes.
Supplementary Table S5: Induction of BnaGLN1 genes expression under low N fertilisation in field grown plants. BnaGLN1 genes
expression level were monitored in limbs and stems from plants grown in field, supplemented (HN) or not (LN) with nitrogen, in young and old
tissues harvested at early flowering and seed filling stages (60 and 71 to 73 on BBCH scale). Data are presented as Log Ratio defined as
log2(LN/HN). * indicate significant differences (Student Test, p<0.05), mean of four plants repeat.
BnaA.GLN1.1.a
BnaC.GLN1.1.a
BnaA.GLN1.2.a
BnaC.GLN1.2.a
BnaA.GLN1.3.a
BnaC.GLN1.3.a
BnaA.GLN1.3.b
BnaC.GLN1.3.b
BnaGLN1.3.c
BnaA.GLN1.4.a
BnaC.GLN1.4.a
BnaA.GLN1.4.b
BnaC.GLN1.4.b
BnGSL1
BnGSL2
Early flowering
Limb
Stem
Old
Young
Old
Young
0.06
0.44*
0.18
1.71*
-0.12
0.23
0.46
1.22*
-0.06
-0.43*
-0.09
-0.06
-0.21
-0.49*
0.11
0.00
0.20
-0.29
-0.33
-0.18
0.27*
-0.05
-0.37
-0.07
1.00*
-0.51*
-0.19
-0.01
0.90
-0.38
-0.32
-0.12
-0.18
-0.05
0.01
-0.01
0.27
0.20
0.47
0.96*
0.28
-0.03
0.65*
0.70*
-0.52
0.22
0.95
0.84*
-0.48
-0.48*
1.97*
0.72*
0.29
-0.13
0.92
0.23
0.14
-0.14
0.86
0.10
Seed filling
Limb
Stem
Old
Young
Old
Young
-0.01
-0.03
-0.83
-0.26
-0.25
0.00
-0.84
-0.38
-0.50
-0.26
-1.53
-0.33
-0.40
0.04
-1.22*
0.12
0.26
0.16
-0.76*
-0.33
0.43*
0.26
-0.74*
0.02
-0.07
0.09
-1.38
-0.21
0.14
0.25
-0.35
0.19
0.37
-0.14
-0.86
-0.17
0.44
0.13
-0.88
-0.41
0.21
0.05
-0.40
-0.23
0.20
-0.45
-0.01
-0.06
0.47
-0.02
0.39*
0.07
0.26
0.34
0.40*
-0.01
0.25
0.17
0.04*
-0.24
* Statistically significant induction under LN (bilateral t test, p-value < 0.05).
Supplementary Figure legends
Supplementary Figure S1: BnaGLN1 genes localisation on A or C genome
using a panel of various Brassica genotypes. Amplification of DNA fragments for
each BnaGLN1 gene on Brassica napus (1, Darmor-bzh; 2, Yudal; 3, Stellar; 4,
Drakkar; 5, Samouraï; 6, Aburamasari; 7, Aviso; 8, Tenor; 9, Express; 10, Montego),
Brassica rapa (11, Z1; 12, C1.3; 13, Chiifu) and Brassica oleracea (14, HDEM; 15,
C102) were performed using specific primers designed for each gene
(Supplementary Table S1) in order to reveal a presence / absence polymorphism
between B. rapa (A) and B. oleracea (C) genomes. Water control is presented (lane
16). Genes in bold letters are also mapped on the genetic map (Supplementary
Figure S3). Stars indicate genes for which there is no agreement with the genetic
mapping performed on segregating populations or for which some results remain
uncertain.
Supplementary Figure S2: BnaGLN1 genes localisation on LG using mono- and
polysomic additional lines. Amplification of specific DNA fragment for each
BnaGLN1 gene was performed on mono- and polysomic additional lines selected
among the descendants from a cross between Brassica napus Darmor-bzh and
Brassica rapa C1.3. Each line is carrying the full A genome (mixture from B. napus
and B. rapa) and one or several C chromosomes originating from Brassica napus
Darmor-bzh. Specific primers designed for each gene (Supplementary Table S1)
were used in order to reveal the presence or absence of specific amplicon on
additional C chromosomes carried by the plant lines (indicated above each lane,
Auger et al., 2009).
Supplementary Figure S3: Positions of BnaGLN1 genes on B. napus genetic
map. Markers corresponding to BnaGLN1 genes mapping positions are indicated
with stars. Cumulative distances between each marker are given in cM on left side of
the chromosome representation. DS, SD and DY are referring to Darmor x Samouraï,
Stellar x Drakkar and Darmor-bzh x Yudal double haploid mapping populations and
reference maps (Lombard and Delourme, 2001; Delourme et al., 2006). Markers are
coloured according to the localisation of their homologous region identified in A.
thaliana (chromosome C1 to C5).
Supplementary Figure S4: Alignments of genomic sequence, deduced mRNA
sequence and contigs’ sequence are reported for each BnaGLN1 gene.
Supplementary Figure S5: Expression of BnaGLN1 genes is modified
depending on nitrate availability and ageing in primary veins of vegetative B.
napus plants.
BnaGLN1 genes relative expression level was monitored in primary veins of six leaf
ranks harvested on vegetative plants grown under low (white bars) or high (black
bars) nitrate conditions. Leaf ranks represented as number 1 (bottom and older leaf)
to 6 (top and younger leaf) showed differential senescence symptoms. Mean and
standard deviation of four plant repeats are shown.
B. napus
1
2
3
B. rapa
4
5
6
7
8
B. oleracea
9 10 11 12 13 14 15 16
BnaA.GLN1.1.a
BnaA.GLN1.2.a
BnaA.GLN1.3.a
BnaA.GLN1.3.b
BnaA.GLN1.3.c
BnaA.GLN1.4.a
BnaA.GLN1.4.b
BnaA.GLN1.5.a
BnaC.GLN1.1.a
BnaC.GLN1.2.a
BnaC.GLN1.3.a
BnaC.GLN1.3.b
BnaC.GLN1.3.c
BnaC.GLN1.4.a
BnaC.GLN1.4.b
BnaC.GLN1.5.a
Supplementary Figure S1: BnaGLN1
genes localisation on A or C genome
using a panel of various Brassica
genotypes:
Amplification of DNA fragments for each
BnaGLN1 gene on Brassica napus (1,
Darmor-bzh; 2, Yudal; 3, Stellar; 4, Drakkar;
5, Samouraï; 6, Aburamasari; 7, Aviso; 8,
Tenor; 9, Express; 10, Montego), Brassica
rapa (11, Z1; 12, C1.3; 13, Chiifu) and
Brassica oleracea (14, HDEM; 15, C102)
were performed using specific primers
designed for each gene (Table S1) in order
to reveal a presence / absence
polymorphism between B. rapa (A) and B.
oleracea (C) genomes.
Water control is presented (lane 16). Genes
in bold letters are also mapped on the
genetic map (supplemental figure S1). Stars
indicate genes for which there is no
agreement with the genetic mapping
performed on segregating populations or for
which some results remain uncertain.
Water
C4
C5
C1 C2 C2
C6
C4 C3 C8
C1 C2 C3 C9 C5 C7 C8 C1 C5 C7 C8 C8 C9 C9
BnaA.GLN1.3.a
A
BnaC.GLN1.3.c
C03
BnaC.GLN1.3.a
C05
BnaC.GLN1.1.a
C06
Water
C1.3
C4
C5
C2 C2 C1
C6
C8 C3 C4
C8 C7 C5 C9 C2 C3 C1 C9 C9 C8 C8 C7 C1 C5
BnaA.GLN1.1.a
A
BnaC.GLN1.3.b
C01
Supplementary Figure S2: BnaGLN1 genes localisation on linkage group using mono- and
polysomic additional lines.
Amplification of specific DNA fragment for each BnaGLN1 gene was performed on mono- and polysomic
additional lines selected among the descendants from a cross between Brassica napus Darmor-bzh and
Brassica rapa C1.3. Each line is carrying the full A genome (mixture from B. napus and B. rapa) and one
or several C chromosomes originating from Brassica napus Darmor-bzh. Specific primers designed for
each gene (Table S1) were used in order to reveal the presence or absence of specific amplicon on
additional C chromosomes carried by the plant lines (indicated above each lane, Auger et al. 2009).
A03_DS
0
4
6
12
14
17
27
31
43
46
50
53
61
67
74
75
76
78
83
87
92
94
103
117
128
129
135
146
W05.1800
E3M3.03
E1M7.08
PFM228
T11.1250
a4NF5c
E4M4.F
E1M7.18
W08.2360
Y02.CD1
PFM193
BnaA.GLN1.3.c
BN466
BN594
O20.1300
BN358b
E1M4.D
C04.1500
A16.3250
E3M6.11
W05.630
E2M4.J
E2M4.I
J04.1295
E2M7.08
E1M3.10
Y04.830
T17.CD1
BN711a
IGF5272c
A05_DS
A06_DY
0
0
26
31
45
52
53
57
58
62
65
68
70
84
94
109
a4NF4b
O19.480
Y01.1640
Y05.1300
At3g22500
At3g22490
P05.2500
BN248
X12.1200
K10.800
BN396b
E1M7.22
E1M3.B
E3M3.06
E1M4.21
BnaA.GLN1.3.a
BN614
E1M5.03
W03.500
PFM175
22
26
29
40
43
51
53
55
60
68
70
71
74
75
76
80
88
99
108
111
112
115
121
134
141
146
149
157
BnaA.GLN1.5.a
BN52344a
BN57463
PFM576
PFM581
sN2837a
Na12D08
sR9411
Bras052
BN51296
sN12750c
Ra1F06b
Ra2D04
BRMS252b
BN57360
CB10065
sN2189b
Na12H06
BN60657
BN50801X
Na10B04b
Na12A08c
Na12G11
sN1939Fb
sN1939Rb
BN50903
BN51495
Bras028
BRMS309b
ChiA.2
PFM191a
Na12G05b
Na12B08
Na10E02b
sS1949a
sNRB93a
Bras115
PFM023
sR12156a
Gai
Bzh
C02_SD
C03_SD
0
0
16
41
50
59
64
68
71
75
80
85
88
93
99
106
110
119
134
136
140
Supplementary Figure S3: Positions of BnaGLN1 genes on B. napus genetic map.
Markers corresponding to BnaGLN1 genes mapping positions are indicated with stars.
Cumulative distances between each marker are given in cM on left side of the
chromosome representation. DS, SD and DY are referring to Darmor x Samouraï, Stellar
x Drakkar and Darmor-bzh x Yudal double haploid mapping populations and reference
maps (Lombard and Delourme, 2001; Delourme et al. 2006). Markers are coloured
according to the localisation of their homologous region identified in A. thaliana
(chromosome C1 to C5).
W19.1450
C18.1250
PFM504
BnaC.GLN1.2.a
J15.1200
T11.1000
A01.1000
S13.450
E3M1.C
E1M5.D
E1M5.E
P14.1720
Y01.2100
P08.1200
L12.800
I11.510
S18.1300
PFM408
N01.1300
E2M7.14
E2M4.E
J04.1400
PFM293
PFM257
12
23
33
39
43
57
58
64
102
119
120
122
125
139
153
158
165
178
204
212
219
221
222
228
246
252
264
288
305
313
320
323
326
327
332
340
354
370
Lap.1C
H07.1700
Bn40C1
E4M4.02
FAE1.C
B15.1130
E3M1.02
E2M6.16
E2M6.17
S4No0233
IGF3222b
PFM92
PFM491
BN04C
At3g21260
BnaC.GLN1.3.c
S2No0522
At3g19920
IGF0193c
C04.1040
IGF5376b
E2M7.E
B07.1200
E2M4.06
E1M1.M
E4M6.05
PFM309
E3M1.05
E1M7.21
E2M6.08
E2M7.10
E1M5.H
E4M4.L
E4M4.16
W09.1500
C09.1500
PFM436
X01.600
B15.900
At3g15690
R06.1360
Y04.870
E1M5.08
E2M4.02
E2M1.05a
R16.1500
Y15.850
PFM243
C18.900
R12.2000
F14.2200
PFM431a
PFM431b
At C1
At C2
At C3
At C4
At C5
Supplementary Figure S4: Alignments of genomic sequence, deduced mRNA
sequence and contig of ESTs sequence are reported for each BnaGLN1 gene.
file:///C:/Users/Camille/Desktop/1380102297.gif[25/09/2013 11:45:38]
file:///C:/Users/Camille/Desktop/1380102297.gif[25/09/2013 11:45:38]
file:///C:/Users/Camille/Desktop/C1a.gif[25/09/2013 11:48:14]
file:///C:/Users/Camille/Desktop/C1a.gif[25/09/2013 11:48:14]
file:///C:/Users/Camille/Desktop/C1a.gif[25/09/2013 11:48:14]
file:///C:/Users/Camille/Desktop/A2a.gif[25/09/2013 11:51:24]
file:///C:/Users/Camille/Desktop/A2a.gif[25/09/2013 11:51:24]
file:///C:/Users/Camille/Desktop/A2a.gif[25/09/2013 11:51:24]
file:///C:/Users/Camille/Desktop/C2a.gif[25/09/2013 11:54:20]
file:///C:/Users/Camille/Desktop/C2a.gif[25/09/2013 11:54:20]
file:///C:/Users/Camille/Desktop/C2a.gif[25/09/2013 11:54:20]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3a.gif[25/09/2013 13:26:32]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3a.gif[25/09/2013 13:26:32]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3a.gif[25/09/2013 13:29:05]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3a.gif[25/09/2013 13:29:05]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3b.gif[25/09/2013 13:27:18]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3b.gif[25/09/2013 13:27:18]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3b.gif[25/09/2013 13:27:18]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3b.gif[25/09/2013 13:29:18]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3b.gif[25/09/2013 13:29:18]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3c.gif[25/09/2013 13:27:46]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A3c.gif[25/09/2013 13:27:46]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3c.gif[25/09/2013 13:29:32]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C3c.gif[25/09/2013 13:29:32]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A4a.gif[25/09/2013 13:28:12]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A4a.gif[25/09/2013 13:28:12]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C4a.gif[25/09/2013 13:29:57]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C4a.gif[25/09/2013 13:29:57]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A4b.gif[25/09/2013 13:28:26]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A4b.gif[25/09/2013 13:28:26]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A4b.gif[25/09/2013 13:28:26]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C4b.gif[25/09/2013 13:30:10]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C4b.gif[25/09/2013 13:30:10]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A5a.gif[25/09/2013 13:28:44]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/A5a.gif[25/09/2013 13:28:44]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C5a.gif[25/09/2013 13:30:22]
file:///C:/Users/Camille/Desktop/Supp%20Figure%20alignements/C5a.gif[25/09/2013 13:30:22]
A
Relative expression units
E
B
C
D
F
G
H
I
J
K
M
N
O
Primary vein ranks
Primary vein ranks
Primary vein ranks
L
P
Primary vein ranks
Supplementary Figure S5: Expression of BnaGLN1 genes is modified depending on nitrate availability and ageing in primary veins of vegetative
B. napus plants.
BnaGLN1 genes relative expression level was monitored in primary veins of six leaf ranks harvested on vegetative plants grown under low (white bars) or
high (black bars) nitrate conditions. Leaf ranks represented as number 1 (bottom and older leaf) to 6 (top and younger leaf) showed differential senescence
symptoms. Mean and standard deviation of four plant repeats are shown.

Classifica assoluta

2014 Results - Satori Camel Run

(2803394)AX31264 後連桿下保護片組