⎇ⓥ⠪䉲䊷䉵⚫੺ ችፒᄢቇᎿቇㇱ‛⾰ⅣႺൻቇ⑼ ᄢፉ䇭㆐਽ ⎇ⓥಽ㊁䇭䇭ಽ㔌Ꮏቇ䋬 ಽሶ⹺⼂ൻቇ ⎇ⓥ᭎ⷐ䇭䇭ᣂⷙ෻ᔕಽ㔌᧚ᢱ 䋨᛽಴೷ 䊶 ๆ⌕೷䋩䈱㐿⊒ 㪠㪤㪘㪚 ᴺ䈮䉋䉎Ⴎಽ䉕฽䉃ᶧ䈎䉌䈱䉝䊮䉶䊥䊮䊶䉦䊦䊉䉲䊮䈱࿁෼ ᫢Լь߻ࡑ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅử‫ׅ‬ӓẴỦỆỊᾎ ᶏᵗᕈࡃࠗࠝࡑࠬߩਛߢ‫ߩߤߥࠝ࠷ࠞޔࡠࠣࡑޔ‬࿁ㆆ㝼߇᦭ߔࠆ ࠕࡦ࠮࡝ࡦ‫ߪࡦࠪࡁ࡞ࠞ߮ࠃ߅ޔ‬ఝࠇߚஜᐽᯏ⢻ࠍ᦭ߒ߹ߔ‫ޕ‬ H3N H N COOCH2 N NH Metal ion ᧄ⎇ⓥߢߪႮಽࠍ฽߻ᾚ᳝߆ࠄߢ߽ ࠕࡦ࠮࡝ࡦ࡮ࠞ࡞ࡁࠪࡦࠍๆ⌕࿁෼ ߢ߈ࠆ̌㊄ዻᜂᜬ᮸⢽̍ߦࠃߞߡ Cation Exchange Resin ࡍࡊ࠴࠼߇Ⴎ㘃ࠍ฽߻ᶧ߆ࠄ߽ ㆬᛯ⊛ߦ࿁෼ߢ߈ࠆߎߣࠍ Fig. 1 Schematic illustration of Car adsorption ᣿ࠄ߆ߦߒ߹ߒߚ‫ޕ‬ by immobilized metal affinity adsorption. (1.36 mmol/g of Cu(II) was immobilized on Cu-IDA resin.) CH2N Car(Cu-IDA,NaCl฽᦭) 100 㘩Ⴎ㕖ሽ࿷ਅ 80 40 CH2COOH Fig. 3 Cation exchange resins and the Cu(II) immobilized resins. 1. Ⴎ㘃฽᦭ṁᶧ߆ࠄߩࡅࠬ࠴ࠫࡦ฽᦭ࡍࡊ࠴࠼ߩ࿁෼ᣇᴺ̍․㗿㧘(2007) 2. T. Oshima, K. Kanemaru, K. Ohe, Y. Baba, Adsorption Behavior of Carnosine and Anserine on Copper (II) immobilized cation exchange resins, J. Ion Exch., in press (2007) Lys Arg Ans His Car Tyr Phe Ile Leu Met Val Cys Ala Gly Glu Pro Ser 0 Thr 20 CH2COOH Fig. 2 Molecular structure of IDA resin. 㘩Ⴎሽ࿷ਅ 60 Asp CH2 CH ㅢᏱߩࠗࠝࡦ੤឵᮸⢽ߪ 㕒㔚⊛⋧੕૞↪ࠍᒁജߣ Ans(Cu-IDA,NaCl฽᦭) ߔࠆߚ߼‫ޔ‬Ⴎ㘃߇ṁߌߚ 60 ṁᶧߢߪࡍࡊ࠴࠼ߩๆ⌕ 40 ജ߇⪺ߒߊૐਅߒ߹ߔ‫ޕ‬ ৻ᣇ‫ޔ‬㊄ዻᜂᜬ᮸⢽ߦࠃ 20 ࠆๆ⌕ߪ㈩૏⚿วߦࠃࠆ 0 ߚ߼‫ޔ‬Ⴎಽ߇౒ሽߔࠆਛ 2 4 6 8 10 12 ߆ࠄߢ߽ࠕࡦ࠮࡝ࡦ࡮ࠞ pHeq Fig. 4 Adsorption profiles of Car and Ans ࡞ࡁࠪࡦࠍๆ⌕ߢ߈߹ߔ on IDA and Cu-IDA ([NaCl] = 100 mM) 㧔(KI‫ޕ‬ Ans(IDA,NaCl฽᦭) 80 C H2 O Car(IDA,NaCl฽᦭) Ads/% H2 C + 100 % Adsorption [%] ᑄ᫈‛ࠍේᢱߣߒߡࠕࡦ࠮࡝ࡦ╬ࠍ ♖⵾ߒ೑↪ߔࠆߚ߼ߦߪ‫ޔ‬ ઁߩᚑಽ߆ࠄ⋡⊛ߩࠕࡦ࠮࡝ࡦ࡮ ࠞ࡞ࡁࠪࡦߛߌࠍ ㆬᛯ⊛ߦขࠅ಴ߔᛛⴚ߇ᔅⷐߢߔ‫ޕ‬ ‫ط‬ửԃớ෩ẦỤỴὅἍἼὅ Ὁ ỽἽἠἉὅử‫ׅ‬ӓẴỦ Fig. 5 Adsorption percent of amino acids and dipeptides from a mix on Cu-IDA (pH 8.0). ࠕࡒࡁ㉄㧝㧣⒳‫ ߮ࠃ߅ޔ‬#PU࡮%CT ߇ฦ O/ ฽߹ࠇߚᷙว ᶧ߆ࠄߩ %W+&# ᮸⢽ߦࠃࠆๆ⌕ታ㛎ߩ⚿ᨐ߇ (KI ߢߔ‫ޕ‬ 㧝㧥⒳ߩࠕࡒࡁ㉄࡮ࡍࡊ࠴࠼ߢ *KU#PU%CT ߇ㆬᛯ⊛ߦ ๆ⌕ߐࠇࠆߎߣ߇␜ߐࠇ߹ߒߚ‫ޕ‬0C%N ߇ O/ ฽߹ࠇࠆᶧ ߆ࠄ߽ߎࠇࠄߩࠕࡒࡁ㉄࡮ࡍࡊ࠴࠼ߪๆ⌕ߐࠇ߹ߔ‫ޕ‬ 䊋䉪䊁䊥䉝䉶䊦䊨䊷䉴⺃ዉ૕ 䈱⺞⵾䈫䉺䊮䊌䉪⾰䈱ๆ⌕ ἢἁἘἼỴἍἽἿὊἋỉࣇኬጞዜನᡯỂ ᭗ܾ᣽ỆἑὅἣἁឋửԈბẴỦ Fig. 7 SEM image of phosphorylated plant cellulose (PPC) at 25,000 magnification 40 O OH P CH2O OH CH2OH O OH O + O OH n H3PO4 (NH2)2CO DMF OH + O OH H2O 40 PPC PC 30 q [μmol g-1] ᧄ⎇ⓥߢߪ࡝ࡦ㉄ൻࡃࠢ࠹࡝ࠕ࠮࡞ࡠ࡯ࠬࠍ ⺞⵾ߒ‫⾰ࠢࡄࡦ࠲ޔ‬ๆ⌕೷ߣߒߡߩๆ⌕․ᕈࠍ ⹏ଔߒߚ‫ޕ‬ ࡝ࡦ㉄ൻࡃࠢ࠹࡝ࠕ࠮࡞ࡠ࡯ࠬߪ࡝ࡦ㉄ൻᐲߩ ඘ᢜߔࠆᬀ‛↱᧪ߩ࡝ࡦ㉄࠮࡞ࡠ࡯ࠬߣᲧセߒߡ ⚂㧞୚ߩๆ⌕ኈ㊂ࠍ␜ߒߚ‫ޕ‬Ყセ⊛ᄢ߈޿ ࠲ࡦࡄࠢ⾰ߪ❫⛽⴫㕙ߢๆ⌕ߐࠇࠆߚ߼‫ޔ‬ᓸ⚦ ❫⛽߆ࠄߥࠆๆ⌕೷߇㜞ኈ㊂ࠍ␜ߔߣ⠨߃ࠄࠇࠆ‫ޕ‬ Fig. 8 SEM image of phosphorylated bacterial cellulose (PBC) at 25,000 magnification PBC BC 30 q [μmol g-1] ㈶㉄⩶ߦઍ⴫ߐࠇࠆ⩶૕߇↥಴ߔࠆ̍ࡃࠢ࠹࡝ ࠕ࠮࡞ࡠ࡯ࠬ̍ߪ‫ޔ‬ᬀ‛↱᧪ߩ࠮࡞ࡠ࡯ࠬߣ Ყセߒߡ 㨪㧘❫⛽᏷ ǴO ߩᓸ⚦ ❫⛽߆ࠄߥࠆ‫ޕ‬ 20 10 20 10 0 0 0 50 100 150 Ceq [μmol dm-3] 200 0 50 100 150 Ceq [μmol dm-3] 200 Fig. 9 Adsorption isotherms of lysozyme onto Fig. 10 Adsorption isotherms of lysozyme onto PPC and PC at 30ºC PBC and BC at 30ºC a) b) n Fig. 6 The representative synthetic route of phosphorylated cellulose 3. T. Oshima, S. Taguchi, H. Fujiwara, K. Ohe, Y. Baba, Adsorption Behaviors of Bioactive Amines and Proteins on Phosphorylated Bacterial Cellulose, J. Ion Exch., in press (2007) Fig. 11 Conceptual illustration of protein adsorption on PPC (a) and PBC (b).