Chapter 6 Appendices Chapter 6 6. Appendices Appendix I - List of instruments Instruments Source Aluminium foil Nice Chemicals Pvt. Ltd. India Animal weighing balance Durga Scientific Pvt. Ltd. India Biosafety cabinet class II Esco, Singapore CO2 incubator RS Biotech, Mini galaxy A, Scotland Cup vortex Devnandan Enterprise, India Cytotoxic safety cabinet Esco, Singapore Deep freezer Skylab Instruments and Engineering Pvt. Ltd. India Digital plethysmometer Medicaid Systems, India Dry Bath Genei, India Electronic balance Shimadzu Corporation, Japan Electrophoresis Power Supply Genei, India ELISA plate reader Thermo, USA Flame photometer Elico, India Fluorescence inverted microscope Leica DM IL, Germany Gas chromatography SIGMA gas chromatograph model-606, Sigma Instruments Pvt. Ltd. India Gel Doc System Genei, India Gel Electrophoresis AssemblyHorizontal Genei, India Heating mantle Durga Scientific Pvt. Ltd. India Hot air oven EIE Instruments Pvt. Ltd. India Hot Plate Nova Instruments Pvt. Ltd. India HPLC HPLC system (JASCO, Japan) 307 Appendices Instruments Source IR Spectrometer FT IR 5300, JASCO, Japan Light microscope OLYUMPUS, trinocular-CX21FS1, USA Magnetic stirrer with hot plate Popular India Pvt. Ltd. India Mass Spectrometer Micromass Q-TOF Micro (Waters Corporation, Milford, MA) Microcentrifuge tube Tarsons Ltd. India Micropipette Accupette, USA Microtips Tarsons Ltd. India Mini Spin Eppendorff, India NMR Spectrometer Bruker AVANCE-400 MHz (Bruker BioSpin, Rheinstetten, Germany) pH meter Lutron Electronic Enterprise Co., Ltd. Taiwan Rat operating table INCORP, India Refrigerated centrifuge Remi Motors Pvt. Ltd. India Refrigerator Samsung, India RO water system Millipore, USA Rotary vaccum evaporator EIE Instruments Pvt. Ltd. India/ Nutronics, India Tissue homogenizer Remi Motors Pvt. Ltd. India Transilluminator Genei, India Ultrasonicator bath EIE Instruments Pvt. Ltd. India Ultrasonicator probe Trans-o-sonic Pvt. Ltd. India UV Spectrophotometer UV-2450, Shimadzu, Japan Water bath Patel Scientific Instruments, India 308 Chapter 6 Appendix II - List of drugs/chemicals Drugs/Chemicals Source 0.25% Trypsin 1X MP Biomedicals, India 1,2-dimethyl hydrazine MP Biologicals, USA 5-fluorouracil Biochem Pharmaceutical Industries, India Agarose Medox Biotech India Pvt. Ltd. India All cell culture media MP Biomedicals, India Animal diet Pranav Agro, India Carbon ink suspension Flow ink and coating Pvt. Ltd. India Cyclophosphamide (Endoxan-N) injection Cadila Healthcare Ltd. India Diagnostic kits Accuare diagnostics Pvt. Ltd. India DMSO cell culture grade Medox Biotech India Pvt. Ltd. India Doxorubicin Sigma Aldrich, India Ethidium Bromide Medox Biotech India Pvt. Ltd. India Glibenclamide Torrent Research Centre, India High purity cholesterol Hi-Media Laboratories, India Millipore polycarbonate membranes Millipore Corporation, Ireland MTT dye MP Biomedicals, India N-nitrosodiethylamine Sigma Aldrich, India Oryzanol Tokyo Chemical Industry Co., Ltd. Tokyo, Japan Penicillin-G and Streptomycin Hi-Media labs, India Soybean phosphatidylcholine Sigma Aldrich, USA Streptozotocin Sigma Aldrich, India Triton X100 Bioworld, USA Trypan blue Sigma Aldrich, India All other chemicals were purchased from CDH Ltd. India 309 Appendices Appendix III - Institutional Animal Ethics Committee (IAEC) approval 310 Chapter 6 Appendix IV- Retro-orbital blood collection from rats 311
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