Polysaccharides analysis
Purification of oligosaccharides
Glycosidic composition
High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector
(HPAEC-PAD)
High Performance Anion Exchange Chromatography with Pulsed Amperometric Detector
(HPAEC-PAD)
Glc A
Glc
Fuc
Gal
Glc
Ara
DP3
Rha
Man Xyl
DP5
Rib
DP4
Gal A
DP6
DP2
DP7
Separation of monosaccharides on a CarboPac PA1 column (Dionex)
Separation of cellodextrins from DP 1 to 7 on a CarboPac PA1 column (Dionex)
Gas Chromatography with Flam Ionisation Detector (FID)
Exclusion Size Chromatography (SEC) with Differential Refractometric Detector
Myo
DP1
GalA
GlcA
DP5
DP7
Man
DP6
DP3
DP 8,9,10
DP2
DP4
GlcA
Glc
GalA
Gal
GlcA
Glc
Gal
Man
GlcA
Gal
GlcNAc
GalA
GlcNAc
Separation and purification of maltodextrins from DP 1 to 10 on a Biogel P2 column (BioRad)
Separation of methyl glycoside trimethylsilyl derivatives of monosaccharides on a HP-5MS column
Glycosidic linkages
High Performance Liquid Chromatography (HPLC) with Differential Refractometric Detector
and UV Detector
Gas Chromatography with Mass Spectrometry Detector (GC/MS)
DP3
PMAA derivatives
1,3,5-tri-O-acetyl-1deuterio-6 deoxy 2,4di-O-methyl Drhamnitol
1,3,5-tri-O-acetyl-1deuterio-2,4,6-tri-Omethyl D-mannitol
1,5-di-O-acetyl-1deuterio-6 deoxy
2,3,4-di-O-methyl Drhamnitol
1,4,5-tri-O-acetyl-1deuterio-2,3,6-tri-Omethyl D-glucitol
DP2
!3)-Man-(1!
DP6
-Rha-(1!
Separation of oligochitins from DP 2 to 6 on a NH2 column
!4)-Glc-(1!
Missions of the Service
Molecular weight and polydispersity of polysaccharides
212 000 Da
with
double
DP5
!3)-Rha-(1!
PMAA derivatives, separated on a Supelco 2380 column
Size Exclusion Chromatography (SEC) equipped
Refractometer, Multi- Angles Light Scattering
DP4
Corresponding
substituted
residue
detection:
Differential
22 800 Da
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Contacts :
[email protected]
[email protected]
Tel : 04 76 03 76 23
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Analysis of a mixture of pullulans on a OH-Pak SB-803HQ Shodex column
Interaction with other
analytical Services
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Application Data No. 1063