Plasmid DNA (pNCBE-Kan-GFP) Safety data sheet

National Centre for Biotechnology Education
Plasmid DNA (pNCBE-Kan-GFP)
Safety data sheet
Prepared in accordance with Regulation (EC) No. 1907/2006 (REACH)
Version 1.4 | Created: 27 March 2014 | Revised: 14 October 2014
SECTION 1. Identification of the substance/mixture and of the company/undertaking
Product identifier
Trade name/Brand
Synonym(s)
REACH number
CAS number
EC number
Plasmid DNA (pNCBE-Kan-GFP)
Not applicable
Deoxyribonucleic acid, aqueous solution
Mixture, not applicable
Mixture, not applicable
Mixture, not applicable
Relevant identified uses
This product is a laboratory preparation for educational use only.
It should be used only for transforming E. coli strain DH10B following the
instructions in the NCBE Bacterial transformation kit (see Section 16).
Uses advised against
This plasmid should NOT be used for transforming other strains of E. coli or
other species of microorganisms because so doing may not be successful and could contravene the Genetically Modified Organisms (Contained Use) Regulations, 2014.
Supplier of the product and
National Centre for Biotechnology Education (NCBE)
of this safety data sheet
University of Reading
2 Earley Gate
Whiteknights
READING
RG6 6AU
United Kingdom
T: 0118 9873743
F: 0118 9750140
E: [email protected]
W: www.ncbe.reading.ac.uk
Manufacturer of the product
National Centre for Biotechnology Education (NCBE)
University of Reading
2 Earley Gate
Whiteknights
READING
RG6 6AU
United Kingdom
T: 0118 9873743
F: 0118 9750140
E: [email protected]
W: www.ncbe.reading.ac.uk
Emergency telephone number
www.ncbe.reading.ac.uk
0118 9873743 (NCBE, University of Reading. 08.30–17.00 weekdays only)
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Safety Data Sheet
SECTION 2. Hazards identification
Classification according to
Regulation (EC) No. 1272/2008 [CLP] Unclassified
Classification according to
Directive 67/548/EEC [DSD] or Directive 1999/45/EC [DPD]Unclassified
Label elements
None required
Other hazardsNone
SECTION 3. Composition/information on ingredients
Name of component
(Synonym)
[CLP index number]
Weight
(%)
EC
(EINECS)
number
CAS
number
REACH
registration
number
Classification
according to
67/548/EEC
[DSD]
Classification
under
Regulation (EC)
No 1272/2008
[CLP]
Water
95
231-791-2
7732-18-5
–
–
–
DNA
(Deoxyribonucleic acid)
5
–
9007-49-2
–
–
–
SECTION 4. First aid measures
Description of first aid measures
General advice
If symptoms persist or in all cases of doubt, seek medical advice. Show this Safety Data Sheet to the doctor in attendance.
If breathed in
Move the victim to fresh air. If the victim has stopped breathing artificial respiration and/or oxygen may be necessary. Call a doctor immediately.
Skin contact
Rinse the affected area with water.
Eye contact
No action necessary
(An eyewash will probably cause more irritation than the DNA solution).
If swallowed
Do NOT induce vomiting. Never give an unconscious person anything to
drink. Most important symptoms, both acute and delayed
Not applicable.
Indication of any immediate medical attention and special treatment needed
Not applicable.
Safety Data Sheet
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www.ncbe.reading.ac.uk
SECTION 5. Firefighting measures
The product is 95% water.
Extinguishing media
Not applicable.
Special hazards arising from the substance or mixture
None known.
Advice for firefighters
Not applicable.
SECTION 6. Accidental release measures
Personal precautions, protective equipment and emergency procedures
Not applicable.
Environmental precautions
Prevent further leakage or spillage if safe to do so.
Methods and material for containment and cleaning up
Wash down a foul water drain. After cleaning, wash away traces with water.
Reference to other sections
See Section 13 for disposal instructions.
SECTION 7. Handling and storage
Precautions for safe handling
Wear a lab coat to prevent spills from damaging clothing.
Conditions for safe storage, including any incompatibilities
Store container tightly closed and frozen at –18 to –20 °C to prevent deterioration of the DNA.
Specific end use(s)
In the NCBE Bacterial transformation kit, the plasmid solution is used to transform bacterial cells.
SECTION 8. Exposure controls/personal protection
Control parameters
Exposure limits
Biological limit values
Derived no effect level
Predicated no effect level
www.ncbe.reading.ac.uk
The product as supplied does not contain any hazardous materials with occupational exposure limits established by regulatory bodies.
The product as supplied does not contain any hazardous materials with occupational exposure limits established by regulatory bodies.
No information available.
No information available.
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Safety Data Sheet
Exposure controls
Exposure limits
Biological limit values
Derived no effect level
Predicated no effect level
The product as supplied does not contain any hazardous materials with occupational exposure limits established by regulatory bodies.
The product as supplied does not contain any hazardous materials with occupational exposure limits established by regulatory bodies.
No information available.
No information available.
SECTION 9. Physical and chemical properties
Physical stateLiquid.
Appearence
Colourless aqueous solution.
OdourOdourless.
pH7.0
Flash point
Does not flash.
Solubility
Not applicable.
SECTION 10. Stability and reactivity
Reactivity
Chemical stability
Possibility of hazardous reactions
Conditions to avoid
Incompatible materials
Hazardous decomposition products
No information available.
When stored at –18 to –20 °C, the product is stable.
No known hazardous reactions.
Do not freeze and thaw repeatedly and avoid excess heat,
as both can lead to degradation of the DNA.
No information available.
No hazardous decomposition products.
SECTION 11. Toxicological information
Accute toxicity
Irritation
Corrosivity
Sensitisation
Repeated dose toxicity
Carcinogenicity
Mutagenicity
Toxicity for reproduction
No information available.
No information available.
No information available.
No information available.
No information available.
No information available.
No information available.
No information available.
SECTION 12. Ecological information
Toxicity
Persistance and degradability
Bioaccumulative potential
Mobility in soil
Results of PBT and vPVB assessment
Other adverse effects
Safety Data Sheet
No information available.
DNA is biodegradable.
No information available.
No information available.
No information available.
None known.
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SECTION 13. Disposal considerations
Waste from residues/unused product
Contaminated packaging
Wash down a foul water drain with plenty of water.
Dispose of in normal waste according to local regulations. The bottle is made of polypropylene and can be recycled.
SECTION 14. Transport information
UN number
Not applicable.
UN proper shipping name
Not applicable.
Transport hazard class
Not applicable.
Packaging group
Not applicable.
Environmental hazards
Not applicable.
SECTION 15. Regulatory information
Not regulated.
Schools and colleges in the UK should refer to Topics in Safety, which includes chapters on both practical microbiology and
work with DNA: Topics in safety (2001) [Third edition] Association for Science Education. ISBN: 0863573169.
An updated (October 2014) version of Chapter 16, covering work with DNA, can be found on the NCBE’s web site: www.
ncbe.reading.ac.uk/transformation and on the Association for Science Education’s web site: www.ase.org.uk
SECTION 16. Other information
Please refer to the Teacher’s guide which accompanies the NCBE Bacterial transformation kit. This can be downloaded from
the NCBE’s Web site: www.ncbe.reading.ac.uk/transformation
Any revisions to this safety data sheet have been to correct the section headings and to improve the layout.
The information given in this safety data sheet is based on the present state of our knowledge.
This safety data sheet has been compiled and is solely intended for this product.
END OF SAFETY DATA SHEET
www.ncbe.reading.ac.uk
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Safety Data Sheet

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