Engineering meets Biology - Technische Universität München

Engineering meets Biology:
Opportunities of Thermoacoustic Imaging
Vasilis Ntziachristos
IBMI
Institute for Biological and Medical Imaging
Chair for Biological Imaging
Technische Universität München & Helmholtz Zentrum München
http://money.cnn.com/pf/best-jobs/
2013
2012
Biology meets Engineering
DISCOVERY
DESIGN & SOLUTIONS
A biologist is a scientist who attempts to discover
underlying mechanisms that govern how
organisms work.
An engineer is concerned with applying scientific knowledge
to develop solutions for problems. Engineers design.
The word engineer is derived from the Latin roots ingeniare
("to devise") and ingenium ("cleverness").
Engineers link scientific discoveries to human needs and
quality of life.[1] In short, engineers are versatile minds
who create links between science, technology, and
society.[5]
Wikipedia , U.S. Department of Labor
Institute for Biological & Medical Imaging (IBMI)
So:
If you have a problem – you call an engineer!
If your problem is :
Bridges and Buildings
Civil Engineers
New Automotive concepts
Mech. / Electrical Engineers
Improving Healthcare
Bio-Engineer
Institute for Biological & Medical Imaging (IBMI)
Biology meets Engineering
Courtesy Prof. Gil Westmeyer
Institute for Biological & Medical Imaging (IBMI)
Biology meets Engineering
Massachusetts Institute of Technology
Cell Design
Compiler
Robotic Gene Synthesis
Smart Cells
Design
if: infection
then: secrete antibiotic
if: cancer cell
then: report or kill
if: atherosclerosis
then: clean
Courtesy Prof. Gil Westmeyer
Institute for Biological & Medical Imaging (IBMI)
NEW JOURNAL – to promote a Photoacoustics community
http://ees.elsevier.com/pacs/
Editor-in-Chief
Vasilis Ntziachristos (Munich, Germany)
Section-Editors
Stanislav Emelianov (US)
Advances in Technology
Sanjiv Sam Gambhir (US)
Nanoparticles and Probes
Daniel Razansky (Germany) Imaging Applications
Editorial Board
• Mark A. Anastasio,
• Bertrand Audoin,
• Paul C. Beard,
• Gerald Diebold
• Rinat O. Esenaliev,
• Matthias Fink
• Martin Frenz,
• Christ Glorieux,
ISSN 2213 – 5979, 2013, Volume 1
http://elsevier.com/locate/pacs
• Pai-Chi Li,
• Matthew O'Donnell,
• Alexander A. Oraevsky,
• Wiendelt Steenbergen,
• Xueding Wang,
• Roger James Zemp,
• Vladimir P. Zharov,
• Quing Zhu
CELLULAR ENGINEERING: Melanin in optoacoustic imaging
A549 tumor bearing mice
Tyrosinase as an MSOT reporter gene
GLV-1h68
GLV-1h324
1
0
7.5
12
18
24
melanin absorption 850nm
Optoacoustic signal
2
PBS
GLV-1h68
GLV-1h324
cryosections
Time post GLV-1h324 infection (h)
tumor
tumor
GLV-1h327
control-rVACV
Vaccinia virus carrying the tyrosinase gene for melanin production
Stritzker J, et.al. PNAS 110(9); 3316-3320 (2013).
Institute for Biological & Medical Imaging (IBMI)
melanin-rVACV
Real-time MSOT handheld imaging
Institute for Biological & Medical Imaging (IBMI)
MSOT: Unprecedented optical imaging performance
3D rendering of Vasculature
HEAD
Spleen
Right
kidney
1
LIVER
Left
kidney
KIDNEY
Feet
Gateau J., et. al. IEEE TMI 2013
Nature Photonics 3, 412-417 (2009)
ACR Chemical Review, 110(5); 2783-2794 (2010)
Nature Methods 7(8); 603-614, (2010)
Nature Protocols 6(8):1121-9 (2011).
Institute for Biological & Medical Imaging (IBMI)
Multi-spectral optoacoustic mesoscopy (MSOM)
<30 micron resolution through 9mm of tissue
Developmental Biology
Mesoscopy
MSOT of the adult zebrafish expressing mCherry in the notochord
In-vivo MSOT data
Histology
590nm
610nm
630nm
In-vivo non-invasive MSOT
500 µm
Rui Ma
Nature Photonics 3, 412-417 (2009)
Institute for Biological & Medical Imaging (IBMI)
ENGINEERING MEETS BIOLOGY
Institute for Biological & Medical Imaging (IBMI)
Systems theory
r +
e
-
C
u
P
y
Systems biology
r +
e
-
C
u
P
y
Thermoacoustic imaging
Reinhart Koselleck-Projekt
Institute for Biological & Medical Imaging (IBMI)
Near-field Radiofrequency Thermoacoustics (NRT)
impulse shape
impulse shape - fitted
1
capacitor
output
water tank
transducer
(a)
voltage (a.u.)
rotation
stage
0.5
0
-0.5
0
20
40
impulse generator
Razansky et al, Med. Phys. 37, 4602-4607 (2010)
Kellnberger et al, Phys. Med. Biol. 56, 3433-3444 (2011)
Institute for Biological & Medical Imaging (IBMI)
60
80
time [ns]
100
120
140
Near-field thermo-acoustic tomography
Resolution characterization
rotation
stage
capacitor
output
water tank
transducer
impulse generator
<100µm
Phys Med Biol. ;56(11):3433-44 (2011)
Medical Physics 37(9); 4602-4607 (2010)
Kellnberger et al, Phys. Med. Biol. 56, 3433-3444 (2011)
Institute for Biological & Medical Imaging (IBMI)
Near-field thermo-acoustic tomography
4
4
4
3
rotation
stage
capacitor
output
4
3
1
water tank
1
1
1
2
2
transducer
4
4
4
4
3
3
1
1
2
1
1
2
impulse generator
Omar M, Kellnberger S, Sergiadis G, Razansky D, Ntziachristos V.
“Near-field thermoacoustic imaging with transmission line pulsers”
Med. Phys. 39(7):4460-6 (2012).
Institute for Biological & Medical Imaging (IBMI)
Frequency domain thermoacoustics
15
Quasi CW
excitation
5
3
-5
-10
-15
1
0
0
5
10
FFT
2
15
(2) Thermoacoustic response – biological tissue
Coil1:
2nd harmonic
response
0
amplitude
amplitude (mV)
10
acoustic response:
2
4
6
frequency (MHz)
8
20
25
time (µs)
10
30
35
40
Kellnberger et al, Opt. Lett. 37, 3423-3425 (2012)
Kellnberger et al, APL in press (2013)
Institute for Biological & Medical Imaging (IBMI)

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