FluoProbes®

FluoProbes®
FT-MS5800
HAA – Biotin
Product Information
Name :
Catalog Number :
Pure Helix aspersa lectin (HAA) from Garden Snail, Biotin conjugated.
FP-MS5800
1mg
Carbohydrate Specificity:
Inhibitor Carbohydrate:
Activity:
N-Acetylgalactosamine
N-Acetylgalactosamine>N-Acetylglucosamine
Less than 0.5 µg/ml will agglutinate A1 or A2 cells. Neuraminidase
treated B and O cells react as strongly as type A cells.
0.01M Tris-HCl -0.15M NaCl, pH 8.0
Buffer:
Chemical Used for Conjugation: Biotinyl N -hydroxysuccinimide ester (BNOHSE)
Stability :
The liquid material is stable for several years when stored in aliquots
with 0.05%sodium azide added as a preservative.
Storage:
Store liquid refrigerated at 5-8°C in aliquots. DO NOT FREEZE!
Directions for use
1.
Wash and block tissue section. We recommend that 1% purified Bovine Serum Albumin (BSA) or
defatted milk powder be used for blocking to prevent non-specific binding. Do not use serum products, they
contain glycoproteins which may lead to high levels of non specific background. After blocking, rinse briefly
with Buffer.
2.
Dilute Biotin Labeled Lectin to desired concentration 5-50 µg/ml using recommended Buffer.
Incubate section or blot for 30-90 minutes at room temperature in a moist chamber. Slightly longer incubation
times may be required if incubation is done at 2-8ºC. Rinse 3 times, 5 minutes each time with recommended
Buffer.
3.
Dilute and incubate Avidin Conjugate according to manufacturer directions.
Notes: Inhibition of lectin binding may be accomplished byusing one of two procedures:
A. Before proceeding to Step #3 incubate lectin treated section or blot with inhibitory carbohydrate for 30-60
minutes at room temperature. NOTE: Complete inhibition may not occur.
B. Preincubate diluted Biotin Labeled Lectin with inhibitory carbohydrate for 30-60 minutes at room
temperature before applying to section or blot.
TROUBLE SHOOTING GUIDE
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References
-
Ishiyama, I., et al. Zeitschrift fur die Gesante Blutforschung. 24: 178-179 (1972)
Related products
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Other conjugated lectins,
Biotin, FITC or AP conjugated, from:
Abrus Precatorius Lectin (Jequirity Bean)
Aegopodium Podagraria Lectin (Ground Elder)
Agaricus Bisporus Lectin (Mushroom)
Allium Sativum Lectin (Garlic)
Anguilla Anguilla Lectin (Fresh Water Eel)
Arachis Hypogaea Lectin (Peanut)
Artocarpus Integrifolia Lectin (Jackfruit) –Jacalin
Bauhinia Purpurea Lectin (Camel's Foot Tree)
Bauhinia Purpurea Lectin
Bryonia Dioica Lectin (White Bryony)
Calystega Sepiem Lectin (Hedge Bindweed Rhizomes)
Canavalia Ensiformis Lectin (Jackbean)
Cancer Antennarius Lectin (California Crab)
Caragana Arborescens Lectin (Pea Tree)
Cicer Arietinum Lectin (Chick Pea)
Colchicum Autumnale Lectin (Meadow Saffron)
Cytisus Sessilifolius Lectin (Portugal Broom)
Datura Stramonium Lectin (Jimson Weed)
Dioclea Grandiflora Lectin (Legume)
Dolichos Biflorus Lectin (Horse Gram)
Erythrina Cristagalli Lectin (Coral Tree)
Euonymus Europaeus Lectin (Spindle Tree)
Galanthus Nivalis Lectin (Snowdrop Bulb)
Glechoma Hederacea Lectin (Ground Ivy)
Glycine Max Lectin (Soybean)
Griffonia Simplicifolia Lectin
Griffonia Simplicifolia Lectin
Helix Aspersa Lectin (Garden Snail)
Helix Pomatia Lectin (Edible Snail)
Hippeastrum Hybrid Lectin (Amaryllis)
Other reagents:
BSA, UPQ84170,
Fluoro-Gel mounting medium, FP-AL2561
-APA–APP-ABA-ASA-AAA-PNA-
Lens Culinaris Lectin (Lentil)
Limax Flavus Lectin (Garden Slug)
Limulus Polyphemus Lectin (Horseshoe Crab)
Lotus Tetragonolobus Lectin (Asparagus Pea)
Lris Hybrid Lectin (Dutch Iris)
Lycopersicon Esculentum Lectin (Tomato)
Maackia Amurensis Lectin
-BPAMaclura Pomifera Lectin (Osage Orange)
-BPAMarasmium Oreades Agglutinin Lectin (Mushroom)
-BDAMorniga G Lectin (Black Mulberry)
–CALSEPA- Morniga M Lectin (Black Mulberry)
-CON ANarcissus Pseudonarcissus Lectin (Daffodil)
-CCAPhaseolus Lunatus Lectin (Lima Bean)
–CAAPhaseolus Vulgaris Lectin (Red Kidney Bean)
-CPAPhaseolus Vulgaris Lectin (Red Kidney Bean)
-CAPhytolacca Americana Lectin (Pokeweed)
-CSAPisum Sativum Lectin (Garden Pea)
-DSAPolygonatum Mulitiflorum Lectin (Commom
Solomon's Seal)
-DGLPolyporus Squamosus Lectin (Mushroom)
-DBARicinus Communis Lectin (Castor Bean)
-ECARicinus Communis Lectin (Castor Bean)
-EEATriticum Vulgare Lectin (Wheat Germ)
-GNATulipa Sp. Lectin (Tulip)
-GHAUlex Europaeus Lectin (Gorse)
-SBAUlex Europaeus Lectin (Gorse)
-GS-IUrtica Dioica Lectin (Stinging Nettle)
-GS-IIVicia Fava Lectin (Fava Bean)
-HAAVicia Villosa Lectin (Hairy Vetch)
-HPAViscum Album Lectin (Mistletoe)
-HHAWisteria Floribunda Lectin (Japanese Wisteria)
-LCH-LFA-LPA-LOTUS-IRA-LEA-MAA-MPA-MOA-MNA-G-MNA-M-NPA-LBA-PHA-E-PHA-L–PWM-PEA-PMA-PSL-RCA-I-RCA-II-WGA-TL-UDA-VFA-VVA-VAA-WFA-
I.e. WGA-biotin, FP-MS5730, WGA-ConA, FP-MS9690 and ConA-FITC, FP-47496A
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FluoProbes®
FT-MS5800
Ordering information
Catalog size quantities and prices may be found at http://www.interchim.com
Please inquire for higher quantities (availability, shipment conditions).
For any information, please ask : FluoProbes® / Interchim; Hotline : +33(0)4 70 03 73 06
Disclaimer : Materials from FluoProbes® are sold for research use only, and are not intended for food, drug, household, or cosmetic use.
FluoProbes® is not liable for any damage resulting from handling or contact with this product.
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