info@ evotec.com www.evotec.com TRANSIENT BULK TRANSFECTION Expression of toxic or complex targets Quick validation of targets variant Instant access to cell based reagents in bulk quantities High target expression levels achievable WE PROVIDE WE PERFORM Host cells Bulk cell production Transient transfection YOU PROVIDE DNA YOU RECEIVE Frozen cells Membranes Proteins info@ evotec.com www.evotec.com TRANSIENT TRANSFECTION BY SCALABLE ELECTROPORATION USING MAXCYTE® STXTM Highly efficient transfection of 10 billion cells in a single batch Simultaneous co-transfection of multiple subunits, auxiliary factors, or reporter constructs. Adjustable expression levels for functional cell based assays or high yield protein expression. Downstream processing of transfected cells to assay ready frozen cells, membranes or purified proteins. EP OF HEK293 NEGATIVE CONTROL EP OF HEK293 WITH KV1.5 8.00 Current [nA] Current [nA] 8.00 4.00 0.00 A: Mock transfected HEK cells 60 mV 60 mV 40 mV 4.00 0.00 Evotec Cell Culture Services Over ten years of experience in custom cell, membrane and protein production Direct communication with project scientists and full access to raw data Reagent production services can be accessed as a standalone function or as integral part of wider collaborative programmes Cell bank management scheme, establishing, propagating, backup storage and distribution of customers cell lines between subsidiaries or partners worldwide 20 mV 0 mV -20 mV B: K V1.5 transfected HEK cells 1 billion HEK293 cells were electroporated either with mock DNA (A) or an expression vector for human potassium channel KV1.5 (B) using MaxCyte® STXTM. Transfection efficacy (top) was analysed by immuno fluorescence labelling of the recombinant ion channel (green). Nuclei were counterstained with DAPI (blue). The function of KV1.5 (bottom) was analysed by automatic patch-clamp using a Port-á-Patch (Nanion) measuring characteristic current profiles in transfected cells.