Sr. o.
List of plates
I. Plate I. Cnllu-. devclopcc.l in D
s11pp lcmentc<l
.,
a.l'pcr.
\\hen MS medium
"ith 2.4-D I mp.II.
l'lntc 2 C111l11s dcn.:lopcd in D 111/du, \\·hrn \1S 111nli111n
supplc111cnt.:d with 2.4 D I mg/I.
1. l'latl' 1. Callus dcvt:loped i11
n
mp<'r ''"lwn MS 1111:diu m
4Xa
s 11 pplc111c11IC'd ''ith 2.·I I> I 111µ/I :rnd kinctm I 111g1l
·I
Plate I C':ill ui. dl'vC'lnpcd i11 D mldn, wl11:11 MS n1cJiLUu
s11ppl\!lllC11tcd with 2, 1- 1) I 111µ/1 (Incl l..111ctit1 I 111g/I.
5.
l'l:nc '\.Callus clcvd o pcd lmrn L> llS!'•'r \\hen MS
5011
medium '11pplc111c:111cd \\1th 2.-1 I> I mµ / l :uid KAP l mg.ti
o
Plate<>- C'allus de\ eloped frrn11 L> 11ilrl11, when M S
11wcli111n supplc111c111c1l '' ith 2.
7.
I 111~ ·1 nml ll,\1' I m1Yl.
l' lntt.: 7. Callu'i clcvclop;:d i11 D a1p1•1· \\h.:11 to.ts
111cdium
!I
t-n
SO:i
~11pplcmcn1ed
\\ith NAA l mg/I ,111d 8/\1' I 111}!/I
Plate X. C;ilh•" de, duped in D lu!tl" "hen J\IS medium
~11pplcme111ctl \\~th N1\J\
9. Plate 9. Mu-.i111u111 callus developed from/)
MIJll)(emcntcd \\llh 2.4-L> I mg/I,
mp<'r
\\hcn MS medium
)·fa
NAA I mg/I and llAP I mg/I.
10. Plate 10. Maximum callus developed from[) asper and n. 111/da. wh~n
MS medium supplemented with 2,4-D Img/I. + Nt\1\ I mgll and
HA P I mgi1.
'i2.1
I 111¥ I nnd H \P I 1111• I
54a
57n.
11. Plate 11 . Fmbryugcnic callus developed in/). al~,. \\11cn
MS medium 'supplcn11.:uted with 2,4 D I mg/1. NAA l mg.II und BAI' I mg/I.
I % surrnsc. -"lOO tux light intt·nsitv :11
n°c i
2 °C'.
57:i
, ., l'latc , ., I 111b1yoyenir <':tllu' dC\'dnf)(<l in /) 111/du When ~I~
1ncdrum
o,upplcmc111cd \\llh ", 1-D I 111~/l NAA l 111p/1 mad OAP I mg.II.
~ % ~~w111f.c. 3000 lux ligh1 i111.!n~ it) u1
'i'c
+ :?
11
c
'\)(a
11 Plate I'\ l ·111h1)1.>g.cnk c::illu.-. Jcyclnpt·tl 111 /J. t1Sp.·1 111111 I.I 111/da
When M<; 111.:Jown s upf)kmc111c1.I \\ 1lh ., I I) I 111Qll on.I
HAP :> ml' ll ••{ u.• -:11no•.c. '000 111' lipl11 1111.:11:>1\\ 01 "7''< ·
14
.! ''(. .
f'llth: 1•1. IVlulltpk <;hMM d t"vclopcd 111 I> '"/><'•when IVIS lwll ~ll!'ll(!lh
ll!>tt
""·d111111 q11 pr lc:m c nlcd "ill! H AI' .' . -."'!.'/!.
1-.
l'l nh! I ~. l\ lu llipk ~1 ..... 1 ~ drv<'IClflCU
ht/) 111!1!0
\\ln·11 :\I' hnll •.1rcnp.1h
'''"
111.:,1111111 ' "l'l'h-mc:1uc:d \\ ilh II \I' '..,'" I' I
"·'!'"'
1>>h
17. l'lak 17. /\1111111.1 11111tc111t a!> \\.:II 11> nl\llttph.• s hool9 d.:vi.'1111w1t 111 I> 11ildC1
h'lh
l o. Plate lo. All>1110 n111tnn1 n~ wc-1111"' mullipk :<hool:< 1.11.:\dvpc,1111 I>
when lull Hl1.:11gth M:-- nwilium ~11rrh·11H'nh·n \'>ilh ll/\I' :?.~ 1111f/I .
\Vh!;'n t\111 'lrt'llJ\lh MS 111\'Chllm '.llppl.-111l'llll'.d \\, llh II \I' I '111p;I
I It
r1a11· I H M11lllpk , Jmoh dt'vdopnl Ill I)
U\(Jt'I' \\
111'11
6811
MS 1m'di11111 ~ upplc111c11tc:d wrth llAI' '.'1111£)101\d 2.·I I) 0.2 u1g/I.
19. Plut~· 19. Multiple shoots deH:lupnl in D wlr/11 \\he11 l\1S mnlium
Mia
:>upplcmcntcd \\Ith ll1\P 2 5 mt!fl and ~A-1> 0.] 111)!/I.
20. Ph11c 20. Multiple shoots <lt:vclopcd in D. a.vpl!r when MS medium
supplemented with OAP 2 5 111~ 'I and '\A.\ 0.2 mg/I
7011
'.!I. Plate 21 Multiple shoots developed in /J 111/dn when ~ I S medium
70a
supplemented with BA I' 2. '\ m1tll r111d NAA 0.2 mg/I
:?:?.
Piute '?J. M11lt1plc llhouts 1kvclopc<l 111 /J n.<:J>N' when MS 111~<liu111
72u
>111rrlcmc111crt with HAI' I 11tv/I I '.!.4-1) I 111g / l a11cl N/\/\ I m p/I.
.,, !'lute 23. Multiple shoms <le\ eloped 111
s uppl e mented
:?·I.
HA I' 1 m e/I
\Vllh
'
I
n
I)
r11ld<1
!'vi~
when
1111:<liu111
I 111i0 und NJ\A I m v / I.
!'1:11.- 7 I Multipk :-h uo1,, d c\ doped lrum
n
"'l"'r \\ lw11
74u
IVl'S 11u•d iw11 ~upplc:mellled "llh B A P ., 0 1111:/1 1111d l "1. \ 0 !I m g/I.
25. l'lnlc 25. l\1111tipl..:
1-l1ot11 " cll'\ <'lnpl'd
.. upplcmcm c u wi1h HA I '
) (,
l'lute
1,,0
; u, M u, imum 11111ll ipk
lrnm r> rule/a
\\hc11
MS """ 1;.,..,
/la
mt•.ll 111ul I \ /\ 0 . 5 m g / I.
-<lwol' dn 1,•k•ped m J.),
' '·'P"' \\hen
7R;I
I\ I ~ m crt i11n1 <:n p1•ll' 1111,·111nl '"llh LI J\ I' 2..5 11111/ I uml en1,;om 11 wnh ' • '.'00111'/ I
">7
Pl<tk 17 l\ l.i:..1111u111 111ult1plc: i.ho oh de\ eloped
in /) 111/t/11
'\!'> m ediwn supplcmcnu:d "1th HA I' 2 . ~ mg'l :uul
28
l'lot~
28.
'"""'ll
<'0<'1"1t\ll1 \q\1~1
:?OOml/l
wlwn I\ 1S mc-d111m .,11ppkmcn1cd
80:i
Plmc ?9. Roots 1nch1cccl in/). F11/da, \\hen lvlS mccli11m -:11pplcmcntcd
ROa
R1ml!.
i111.Jucc<l in {),
fl.\'/WI'
\\ ith N/\A l. .'i 111g/l.
11)
"ith NAA U mg.II.
10. Plate 30. Roots induced in D aspc>r\\ h~n ti.IS 111cdium supplemented
81a
with JBJ\ 1.0 mg/I.
31. Plate l 1. Roots induced in D. tulda when MS medium
81a
~uriplemented
with IBA 1.0 mg/I.
32. Pl ate 32. Rnols induce from D. n.1·1wr \\'h.;n lrnlf stl'ength MS medium
83n
s uppkmc ntc d w ith J/\/\ 1.0 m g/I.
33 . Plate 33. R oots induce fro m D. tu/do, \\he11 half s lTc n g lh MS medium
83a
s u p pkmc n tcd w ith l/\.J\ I .0 mg/ I.
.14 .
P l111e 14. Roo1 s induced from /) asper w h en MS m edi u111 s uppleanc 11te<l
!Uh
w i1h I AA 1.0 m g/I
15.
l'l111 c
"itla
>7 .
~'5.
R nnls induced fro m D . 111/da. " lie n MS 111ed iL1111 s 11pplemen1ed
!nh
I A/\ 1.0 mg/ I.
P int ..- 17 Plu11l:. lru11s krL d i11 pol y huµ,s . \\'hich wc1<' m ni n1:oi1 ,.·d i11
0
8 /u
8.IC'\.~ 11 IJ LH l t-.C .
'I X
{9
l'l mc 3X
/11 \'t/ro .-uis<:d ph111tkls uf fJ. 111/da u flc r I 5 d11ys
!'late 39. l'lants 1rnns!C1c d in pol) baµ:,_ \\ liidt were 111 u i11 Lui11e<l in
!!1 ('('I) h ull!-1C.
X7:a
x /11
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