Instrument Name
_______________________________________
Start Date
_______________________________________
Flow Cell (A or B)
Flow Cell A
Flow Cell ID
__________________________________________
Run Folder Name
_______________________________________
Run Type
Single-Read Run ____________
Indexed Single-Read Run ___________
Paired-End Run ____________
Indexed Paired-End Run ___________

Flow Cell B

For access to reagent lot numbers, see my.illumina.com/MyIllumina/CertificateSearch.
C
1
Box 1 (15° to 30°C) Kit ID (RGT-#####)
_______________________
Box 2 (-25° to -15°C) Kit ID (RGT-#####)
_______________________
Box (-25° to -15°C) Kit ID (RGT-#####)
_______________________
Box (-25° to -15°C) Kit ID (RGT-#####)
_______________________
Gaskets (package of 4) ID (RGT-#####)
_______________________
Do not prime SBS reagents if the flow cell is clustered on the HiSeq 2500 or HiSeq 1500.
Lane 1
-100.0%
2.5 ml
Lane 2
-100.0%
2.5 ml
Part # 15059756 Rev. A
Page 1 of 3
Expected volumes include 1 ml priming volume per reagent position.

1
IMT
2
Water
3
USM
4
Water
5
0.00

0.124 ml/cycle
26.24 ml
63.44 ml
0.00
0.15 ml/cycle
31.3 ml
76.3 ml
USB
0.00
0.368 ml/cycle
78.206 ml
188.606 ml
6
USB
0.00
0.28 ml/cycle
57 ml
141 ml
7
CRM
0.00
0.22 ml/cycle
45.22 ml
111.22 ml
CWM
0.00
0.238 ml/cycle
48.888 ml
120.288 ml
8
Load 25 ml PW1 or laboratory-grade water
Load 25 ml PW1 or laboratory-grade water


10
PW1
Load 10 ml PW1 or laboratory-grade water
11
PW1
Load 10 ml PW1 or laboratory-grade water
12
PW1
Load 10 ml PW1 or laboratory-grade water
13
AMS
0.00
5.18 ml
14
FPM
0.00
2.57 ml
15
FDR
0.00
2.97 ml*
16
HP9
0.00
1.20 ml
17
HP12
0.00
1.20 ml
18
HP10
0.00
1.20 ml
19
FLS
0.00
1.25 ml


*
Expected volume of FDR is less than 2.97 ml for single-indexed or non-indexed reads.
10
FRM
0.00
1.20 ml
11
FLM2
0.00
1.25 ml
12
FLM1
0.00
1.25 ml
13
AMS
0.00
6.91 ml
FPM
0.00
3.24 ml
15
FDR
0.00
4.04 ml*
16
HP11
0.00
1.20 ml
17
HP12
0.00
1.20 ml
18
HP10
0.00
1.20 ml
19
PW1
14
*
Load 10 ml PW1 or laboratory-grade water
Expected volume of FDR is less than 4.04 ml for single-indexed or non-indexed reads.
Part # 15059756 Rev. A
Page 2 of 3
Wash volumes include positions on the library loading station. Allow ±10% delivered volumes.
1–20
60.2 ml
1–20
19 ml
Name:
____________________
Date:
____________________
Signature:
____________________
Part # 15059756 Rev. A
Page 3 of 3

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