Proteasomes / Activators / Subunits Proteasomes / Activators / Subunits E1 Activating Enzymes E2 Conjugating Enzymes E3 Ubiquitin Ligases Ubiquitin / SUMO Chains Ubiquitin / Ubiquitin-like Proteins / Mutants Deubiquitinating Enzymes Ubiquitin / UBL Derivatives Proteasome / Deubiquitinase Inhibitors Fluorescent Peptide / Protein Substrates Ubiquitin Binding Proteins Antibodies Kits Protein Expression / Purification Reagents DNA/Protein Ladders UBPBio, LLC 12635 E. Montview Blvd. Aurora, CO 80045, USA [email protected] www.ubpbio.com HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES ABOUT UBPBIO The Ubiquitin-Proteasome Biotechnologies, LLC (UBPBio) was co-founded by experienced scientists and private investors in 2011. We are dedicated to providing the academic and industrial communities with high quality and affordable reagents to accelerate discoveries on the ubiquitin-proteasome system and related pathways. Our products were launched to the ubiquitin-proteasome community at the end of 2012. We currently carry 270 products in 15 categories. We plan to manufacture approximately 200 new products by the end of 2015. We greatly appreciate many scientists who have trusted our hard work and we are proud of our contribution to their important discoveries. Please visit www.ubpbio.com for our product details and special offers. HOW TO ORDER Method 1 To order online (for customers in the United States and Canada only) Method 2 Step 1: Log into your UBPBio account at www.ubpbio.com. If you are a first-time user, please take 3-5 minutes to register first. Step 2: Browse the Product List or search the website to find the products. Step 3: Edit the quantity and add all items to cart. Step 4: Review and submit your order at My Cart using credit card or PO. To order by Fax or Email Open the Order Center tab at www.ubpbio.com homepage to download the order form (PDF or MS Word ). Fax your order to 1-800-682-6801, or email to [email protected]. Method 3 To order by quotation Step 1: Open the Request A Quote tab at www.ubpbio.com home page. Step 2: Fill in and submit the online quotation form. Step 3: We will issue a quotation via email within one business day. Step 4: Complete the issued quotation form with payment information (credit card, PO or wire transfer). Fax your order to 1-800-682-6801 or email to [email protected]. Method 4 To order via distributors We have experienced distributors in Belgium/Netherlands/Luxembourg, China, France, Germany, India, Israel, Japan, Israel, Taiwan and United Kingdom (see last page of this booklet for contact information). www.ubpbio.com HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES FEATURED PRODUCTS Highly Purified Active Proteasomes Human 26S proteasome (catalog A1100/A1101); Bovine 26S proteasome (catalog A1200/A1201); Bovine 20S proteasome (catalog A1400/A1401); Bovine immuno 20S proteasome (catalog A1500/A1501); Bovine 19S regulatory particle (PA700) (catalog A1300/A1301) Applications: Assay proteasomal activities; Reconstitute proteasomal degradation; Screen proteasome inhibitors Doubly-capped 26S Singly-capped 26S Doubly-capped Singly-capped 26S 19S A Purified proteasomes on native gel A: Human 26S proteasome B: Bovine 26S proteasome C: Bovine 20S proteasome D: Bovine immuno 20S proteasome E: Bovine 19S regulatory particle (PA700) B C D E 26S Proteasome Purification Kit (Catalog J4310/J4320) Features: Rapid purification of endogenous 26S proteasome in cells or tissue lysates (approximately 8 hours) Suitable for small and large scale of purifications Produce active 26S proteasome for activity assays A. Coomassie stained SDS-PAGE of 3 µg purified human 26S proteasomes from 293T cells. B. Coomassie stained native-PAGE of 3 μg purified human 26S proteasomes from 293T cells. C. Solution peptidase assay using SUC-LLVY-AMC as the fluorogenic substrate to determine the chymotrypsin-like activity of purified human 26S proteasome. www.ubpbio.com HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Di-Ub Explorer Kit (Catalog J2000) (Containing highly purified linear, K6, K11, K27, K29, K33, K48 and K63 Ub2) Applications: Determination of Ub linkage specificity of deubiquitinating enzymes and Ub-binding proteins Coomassie-stained SDS-PAGE of 1 g each diUb A: Linear diUb B: K6-linked diUb C: K11-linked diUb D: K27-linked diUb E: K29-linked diUb F: K33-linked diUb G: K48-linked diUb H: K63-linked diUb I: Ubiquitin 17 kDa A B C D E F G H I PolyUb Chain Capture Kit (Catalog J4460) PolySUMO Capture and Release Kit (Catalog J4410) Applications: Rapid enrichment of the majority of polyubiquitinated proteins in cell or tissue lysates; Identification of polyubiquitinated proteins by immunoblotting or mass spectrometry after enrichment Applications: Rapid enrichment of polysumolyated proteins in cell or tissue lysates; Released polysumolyated proteins are at native states suitable for downstream assays kDa kDa 1 GST 2 3 GST – 4XSIM 1 2 3 170 100 IB: Ub 55 35 25 15 IB: K48-specific IB: SUMO 2/3 5 mg HeLa cell lysates were mixed with 0.2 mg GST (lane 1, negative control) or GST-tagged Ub binding proteins (lane 2). 10% eluates were separated on SDS-PAGE, followed by immunoblotting of Ub or K48-specific Ub chains. www.ubpbio.com GST pulldown was used as a negative control in these assays. Lane 1: 293T cell lysates before pulldown Lane 2: 293T cell lysates after pulldown Lane 3: Eluates HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Proteasome and Proteasomal DUB Inhibitors > 98% purity and low price MG132 (catalog F1100) - the mostly used proteasome inhibitor in basic science researches. PS341 (catalog F1200) – the first FDA approved proteasome inhibitor for the treatment of multiple myeloma. Carfilzomib (catalog F1300) – a derivative of epoxomicin, recently approved by the FDA for the treatment of multiple myeloma. Epoxomicin (catalog F1400) – a highly specific, potent and irreversible inhibitor of the proteasome. ONX-0914 (catalog F1410) – a selective immunoproteasome inhibitor at low concentrations (0.1 - 0.3 M). IU1 (catalog F4100)– an inhibitor of proteasome-associating DUB USP14. b-AP15 (catalog F2100) – an inhibitor that targets proteasome-associating DUBs USP14 and UCH37. Protein and Antibody Affinity Purification Resins Resin Binding Capacity Nickel XPure Agarose > 26 mg / ml gel Glutathione Agarose > 10 mg GST / ml gel Streptavidin XPure Agarose > 330 nmol free biotin / ml gel Protein A Agarose ~ 25 mg human IgG / ml gel Protein G XPure Agarose ~ 20 mg human IgG / ml gel Ub Derivatives Ub aldehyde (catalog F3100) – a reversible cysteine protease DUB inhibitor HA-Ub-VME (catalog M1010) – an irreversible cysteine protease DUB inhibitor Biotin-Ub-PA (catalog M1020) – an irreversible cysteine protease DUB inhibitor, but the formed covalent bond can be cleaved by acid treatment (5% TFA), well suitable for identification of modified DUBs by mass spectrometry. Ub-AMC (catalog M3010) – a fluorogenic substrate of DUBs. AMC fluorescence can be determined at excitation/emission wavelengths of 360 nm/460 nm. Ub-Rhodamine 110 (catalog M3020) – a fluorogenic substrate of DUBs. Rhodamine 110 fluorescence can be determined at excitation/emission wavelengths of 485 nm/535 nm. TAMRA-Lys(Ub)-Gly (catalog M3030) – A DUB substrate for fluorescence polarization assay using excitation/emission wavelengths at 530 nm/580 nm. www.ubpbio.com HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES CATALOGS Proteasomes / Activators / Subunits CAT. # A1100 A1101 A1200 A1201 A1300 A1301 A1400 A1401 A1500 A1501 A2100 A2101 A2200 A2201 A2300 PRODUCT NAME Human 26S proteasome Human 26S proteasome Bovine 26S proteasome Bovine 26S proteasome Bovine 19S (PA700) Bovine 19S (PA700) Bovine 20S proteasome Bovine 20S proteasome Bovine immuno 20S proteasome Bovine immuno 20S proteasome 6xHis-PA28alpha 6xHis-PA28alpha 6xHis-PA28beta 6xHis-PA28beta 6xHis-PA28gamma SIZE 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 100 µg 25 µg 100 µg 25 µg PRICE $225 $420 $180 $300 $180 $300 $105 $180 $150 $250 $80 $160 $80 $160 $80 CAT. # A2301 A3300 A3301 A3310 A3311 A3400 A3401 A3500 A3501 A3600 A3601 A3710 A3711 A3900 A3901 PRODUCT NAME 6xHis-PA28gamma GST-S5a GST-S5a S5a S5a 6xHis-Adrm1 6xHis-Adrm1 6xHis-Uch37 6xHis-Uch37 Adrm1/Uch37 Adrm1/Uch37 6xHis-Usp14 6xHis-Usp14 6xHis-PI31 6xHis-PI31 SIZE 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg PRICE $160 $80 $150 $80 $150 $80 $150 $80 $150 $80 $150 $80 $150 $95 $225 SIZE 25 µg 100 µg 25 µg 100 µg PRICE $95 $300 $95 $300 SIZE 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg PRICE $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 E1 - Activating Enzymes CAT. # B1100 B1101 B1110 B1111 PRODUCT NAME UBE1 UBE1 GST-UBE1 GST-UBE1 SIZE 25 µg 100 µg 25 µg 100 µg PRICE $95 $300 $95 $275 CAT. # B1300 B1301 B1400 B1401 PRODUCT NAME 6xHis-SAE1/SAE2 6xHis-SAE1/SAE2 APPBP1/6xHis-UBA3 APPBP1/6xHis-UBA3 E2 - Conjugating Enzymes CAT. # C1100 C1101 C1200 C1201 C1300 C1301 C1400 C1401 C1500 C1501 C1600 C1601 C1700 C1701 C1800 C1801 C1900 PRODUCT NAME 6xHis-UbE2A (Rad6A) 6xHis-UbE2A (Rad6A) 6xHis-UbE2B 6xHis-UbE2B 6xHis-UbE2C (UbcH10 ) 6xHis-UbE2C (UbcH10 ) 6xHis-UbE2D1 (UbcH5A) 6xHis-UbE2D1 (UbcH5A) 6xHis-UbE2D2 (UbcH5B) 6xHis-UbE2D2 (UbcH5B) 6xHis-UbE2D3 (UbcH5C) 6xHis-UbE2D3 (UbcH5C) 6xHis-UbE2D4 (UbcH5D) 6xHis-UbE2D4 (UbcH5D) 6xHis-UbE2E1 (UbcH6) 6xHis-UbE2E1 (UbcH6) 6xHis-UbE2E2 (UbcH8) SIZE 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg PRICE $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 CAT. # C2401 C2500 C2501 C2800 C2801 C2900 C2901 C3000 C3001 C3100 C3101 C3200 C3201 C3400 C3401 C3500 C3501 www.ubpbio.com PRODUCT NAME 6xHis-UbE2H (UbcH2) 6xHis-UbE2I 6xHis-UbE2I GST-UbE2K (E2-25K) GST-Ube2K (E2-25K) 6xHis-UbE2L3 6xHis-UbE2L3 6xHis-UbE2L6 6xHis-UbE2L6 6xHis-UbE2M (UbcH12) 6xHis-UbE2M (UbcH12) 6xHis-UbE2N (6xHiUbc13/Uev1a) 6xHis-UbE2N (6xHisUbc13/Uev1a) 6xHis-UbE2Q2 6xHis-UbE2Q2 6xHis-UbE2R1 (UbcH3, Cdc34) 6xHis-UbE2R1 (UbcH3, Cdc34) HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES CAT. # C1901 C2000 C2001 C2100 C2101 C2200 C2201 C2300 C2301 C2400 PRODUCT NAME 6xHis-UbE2E2 (UbcH8) 6xHis-UbE2E3 (UbcH9) 6xHis-UbE2E3 (UbcH9) 6xHis-UbE2F 6xHis-UbE2F 6xHis-UbE2G1 6xHis-UbE2G1 6xHis-UbE2G2 6xHis-UbE2G2 6xHis-UbE2H (UbcH2) SIZE 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg PRICE $190 $85 $190 $85 $190 $85 $190 $85 $190 $85 CAT. # C3600 C3601 C3700 C3701 C3800 C3801 C3900 C3901 C4000 C4001 PRODUCT NAME 6xHis-UbE2R2 6xHis-UbE2R2 6xHis-UbE2S 6xHis-UbE2S 6xHis-UbE2T 6xHis-UbE2T 6xHis-UbE2V2 6xHis-UbE2V2 6xHis-UbE2W 6xHis-UbE2W SIZE 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg PRICE $85 $190 $85 $190 $85 $190 $85 $190 $85 $190 PRODUCT NAME HSP70 HSP70 ITCH GST-UbE3A (E6AP) SIZE 25 µg 100 µg 25 µg 100 µg PRICE $95 $195 $195 $195 SIZE 25 µg 100 µg 25 µg 100 µg 25 µg 50 µg 25 µg 25 µg 50 µg 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg 100 µg 25 ug 25 ug 25 ug 25 ug 25 µg 25 µg 25 µg PRICE $95 $200 $115 $220 $120 $210 $195 $120 $210 $95 $95 $95 $95 $95 $150 $150 $150 $95 $250 $250 $250 $250 $85 $150 $150 E3 - Ubiquitin Ligases CAT. # K1100 K1101 K1200 K1201 PRODUCT NAME GST-RNF4 GST-RNF4 6xHis-CHIP 6xHis-CHIP SIZE 50 µg 250 µg 50 µg 250 µg PRICE $95 $195 $95 $195 CAT. # K1300 K1301 K1400 K1410 Ubiquitin and SUMO Chains CAT. # D1100 D1101 D1200 D1201 D1300 D1301 D1400 D1500 D1501 D1600 D1601 D1700 D1701 D2100 D2101 D2200 D2201 D2300 D2301 D2400 D2401 D2500 D2501 D2600 D2601 PRODUCT NAME K48-Ub2 K48-Ub2 K48-Ub3 K48-Ub3 K48-Ub4 K48-Ub4 K48-Ub5 K48-Ub(2-8) K48-Ub(2-8) Non-cleavable K48 Ub (2-6) Non-cleavable K48 Ub (2-6) 6xHis-Non-cleavable K48 Ub (2-4) 6xHis-Non-cleavable K48 Ub (2-4) K63-Ub2 K63-Ub2 K63-Ub3 K63-Ub3 K63-Ub4 K63-Ub4 K63-Ub5 K63-Ub5 K63-Ub6 K63-Ub6 K63-Ub(2-12) K63-Ub(2-12) SIZE 25 µg 100 µg 25 µg 50 µg 25 µg 50 µg 25 µg 25 µg 50 µg 25 µg 100 µg 25 µg 100 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg 25 µg 50 µg PRICE $65 $125 $65 $125 $120 $210 $150 $65 $110 $95 $200 $115 $220 $65 $115 $120 $210 $120 $210 $120 $210 $120 $210 $65 $125 CAT. # D2700 D2701 D2800 D2801 D3100 D3101 D3200 D3300 D3301 D4100 D4110 D4210 D4220 D4230 D4400 D4410 D4430 D4300 D5110 D5210 D5310 D5410 D9100 D9110 D9130 www.ubpbio.com PRODUCT NAME Non-cleavable K63 PolyUb Chain (2-10) K63 PolyUb Non-cleavable Chain (2-10) 6xHis-Non-cleavable K63 PolyUb Chain (2-4) K63 6xHis-Non-cleavable PolyUb K11-Ub2Chain (2-4) K11-Ub2 K11-Ub3 K11-Ub(n≥3) K11-Ub(n≥3) GST-linear Ub2 GST-linear Ub4 6xHis-linear Ub4 6xHis-linear Ub6 6xHis-linear Ub8 Non-cleavable Linear Ub6 6xHis-Non-cleavable Lin. Ub6 6xHis-Non-cleavable Lin. Ub4 Linear Ub2 K6-linked diUb K27-linked diUb K29-linked diUb K33-linked diUb Di-SUMO2 SUMO2 Chain (2-6) Non-cleavable SUMO2 Chain (2-6) HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Ubiquitin / Ubiquitin-like Proteins / Mutants CAT. # E1100 E1110 E1120 E1200 E1210 E1220 E1300 E1310 E1320 E1400 E1500 E1501 E1610 E1620 E1630 E1640 E1650 E1660 E1670 E1680 E1710 E1720 E1730 E1740 E1750 E1760 E1770 E1780 PRODUCT NAME Ubiquitin Ubiquitin(1-75) Ubiquitin (+1) GST-Ubiquitin GST-Ubiquitin(1-75) GST-Ubiquitin (+1) 6xHis-Ubiquitin 6xHis-Ubiquitin(1-75) 6xHis-Ubiquitin (+1) HA-Ubiquitin 6xHis-Ub-GFP 6xHis-Ub-GFP Ubiquitin (K0) Ubiquitin (K6R) Ubiquitin (K11R) Ubiquitin (K27R) Ubiquitin (K29R) Ubiquitin (K33R) Ubiquitin (K48R) Ubiquitin (K63R) 6xHis-Ubiquitin (K0) 6xHis-Ubiquitin (K6R) 6xHis-Ubiquitin (K11R) 6xHis-Ubiquitin (K27R) 6xHis-Ubiquitin (K29R) 6xHis-Ubiquitin (K33R) 6xHis-Ubiquitin (K48R) 6xHis-Ubiquitin (K63R) SIZE 5 mg 1 mg 100 µg 1 mg 1 mg 100 µg 1 mg 1 mg 100 µg 1 mg 25 µg 100 µg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg PRICE $55 $70 $195 $95 $90 $225 $55 $70 $195 $55 $85 $195 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 CAT. # E1820 E1830 E1840 E1850 E1860 E1870 E1880 E1900 E1910 E1920 E1930 E1940 E2100 E2200 E2201 E3100 E3110 E3120 E3200 E3210 E3220 E3230 E3240 E3250 E3300 E3310 E3320 E4100 PRODUCT NAME 6xHis-Ubiquitin (K6 only) 6xHis-Ubiquitin (K11 only) 6xHis-Ubiquitin (K27 only) 6xHis-Ubiquitin (K29 only) 6xHis-Ubiquitin (K33 only) 6xHis-Ubiquitin (K48 only) 6xHis-Ubiquitin (K63 only) Ubiquitin (I44A) 6xHis-Ubiquitin (I44A) GST-Ubiquitin (I44A) Ubiquitin (L73P) 6xHis-Ubiquitin (L73P) 6xHis-ISG15 6xHis-mISG15-GFP 6xHis-mISG15-GFP SUMO1 GST-SUMO1 6xHis-SUMO1 SUMO2 GST-SUMO2 6xHis-SUMO2 SUMO2 (Q90P) GST-SUMO2 (Q90P) 6xHis-SUMO2 (Q90P) SUMO3 GST-SUMO3 6xHis-SUMO3 6xHis-Nedd8 SIZE 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 1 mg 500 µg 25 µg 100 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg 500 µg PRICE $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $70 $150 $85 $195 $150 $100 $150 $150 $100 $150 $150 $150 $150 $150 $100 $150 $135 SIZE 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 25 µg 25 µg 50 µg PRICE $150 $80 $150 $80 $150 $80 $150 $235 $195 $195 $195 Deubiquitinating Enzymes CAT. # H1100 H1101 H1110 H1111 H1200 H1201 H1210 H1211 H3000 H3001 H3010 PRODUCT NAME UCHL1 UCHL1 GST-UCHL1 GST-UCHL1 UCHL3 UCHL3 GST-UCHL3 GST-UCHL3 OTUB1 OTUB1 GST-OTUB1 SIZE 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg PRICE $80 $150 $80 $150 $80 $150 $80 $150 $80 $150 $80 CAT. # H3011 H3100 H3101 H3110 H3111 H3210 H3211 H5700 H5710 H6110 H6500 www.ubpbio.com PRODUCT NAME GST-OTUB1 OTUB2 OTUB2 GST-OTUB2 GST-OTUB2 GST-A20(1-365) GST-A20(1-365) USP7 GST-USP7 GST-USP11 GST-USP15 HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Proteasome / Deubiquitinase Inhibitors CAT. # F1100 F1101 F1102 F1200 F1201 F1202 F1300 F1301 F1400 PRODUCT NAME MG-132 MG-132 MG-132 PS-341 PS-341 PS-341 Carfilzomib (PR-171) Carfilzomib (PR-171) Epoxomicin SIZE 5 mg 25 mg 100 mg 5 mg 25 mg 100 mg 5 mg 25 mg 100 µg PRICE $55 $200 $600 $70 $220 $640 $90 $295 $115 CAT. # F1401 F1410 F1411 F2100 F2101 F3100 F4100 F4101 PRODUCT NAME Epoxomicin ONX-0914 (PR-957) ONX-0914 (PR-957) b-AP15 b-AP15 Ub aldehyde IU1 IU1 SIZE 500 µg 5 mg 25 mg 2 mg 10 mg 50 µg 5 mg 25 mg PRICE $350 $125 $395 $55 $135 $105 $65 $240 SIZE 25 mg 50 mg 250 mg 50 ug 50 ug PRICE $240 $120 $350 $195 $195 SIZE 25 µg 100 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg PRICE $80 $150 $105 $215 $105 $215 $105 $215 $105 $215 $105 $215 $105 $215 SIZE 50 µg 1 mg 1 mg 1 mg 1 mg PRICE $205 Inquiry Inquiry Inquiry Inquiry Fluorescent Peptide / Protein Substrates CAT. # G1100 G1101 G2100 G2101 G3100 PRODUCT NAME Suc-LLVY-AMC Suc-LLVY-AMC Z-LLE-AMC Z-LLE-AMC Boc-LRR-AMC SIZE 5 mg 25 mg 5 mg 25 mg 5 mg PRICE $55 $240 $95 $320 $60 CAT. # G3101 G4100 G4101 G7000 G7010 PRODUCT NAME Boc-LRR-AMC Gly-AMC Gly-AMC Polybiquitinated E2-25K Polyubiquitinated UbE2S Ubiquitin Binding Proteins CAT. # I1100 PRODUCT NAME GST I1110 I1111 I1200 I1201 I1300 I1301 I1310 I1311 I1400 I1401 I1410 I1411 I1500 I1501 GST-hHR23B (Rad23B) GST-hHR23B (Rad23B) VCP (bovine) VCP (bovine) GST-4xSIM GST-4xSIM 6xHis-4xSIM 6xHis-4xSIM GST-ZnF-UBP GST-ZnF-UBP 6xHis -ZnF-UBP 6xHis -ZnF-UBP GST-NEMO GST-NEMO SIZE 1 mg 25 µg 100 µg 25 µg 50 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg 100 µg 500 µg 25 µg 100 µg PRICE $95 $80 $150 $120 $210 $95 $195 $95 $195 $95 $195 $95 $195 $80 $150 CAT. # I1510 I1511 I1520 I1521 I1530 I1531 I1610 I1611 I1620 I1621 I1710 I1711 I1720 I1721 PRODUCT NAME GST-NEMO (L329P) GST-NEMO (L329P) 6xHis-NEMO (UBAN) 6xHis-NEMO (UBAN) GST-NEMO (UBAN) GST-NEMO (UBAN) 6xHis-S5a (UIM) 6xHis-S5a (UIM) GST-S5a (UIM) GST-S5a (UIM) 6xHis-TAB2 (NZF) 6xHis-TAB2 (NZF) GST-TAB2 (NZF) GST-TAB2 (NZF) Ubiquitin / UBL Derivatives CAT. # M1010 PRODUCT NAME HA-Ub-VME M1020 F3100 M3010 M3020 Biotin-Ub-PA Ub aldehyde Ub-Amc Ub-Rhodamine 110 SIZE 25 µg 25 µg 50 µg 50 µg 50 µg PRICE $205 $205 $105 $200 $240 CAT. # M3030 M3040 M3050 M3060 M3070 www.ubpbio.com PRODUCT NAME TAMRA-Lys(Ub)-Gly TAMRA-Lys(Nedd8)-Gly TAMRA-Lys(SUMO1)-Gly TAMRA-Lys(SUMO2)-Gly TAMRA-Lys(ISG15)-Gly HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Antibodies CAT. # Y1010 Y1011 Y1020 Y1021 Y1030 Y1031 Y1040 Y1041 Y1050 Y1051 Y1060 PRODUCT NAME Anti-6xHis Anti-6xHis Anti-GST Anti-GST Anti-GFP Anti-GFP Anti-GAPDH Anti-GAPDH Anti-beta-Actin Anti-beta-Actin Anti-beta-Tubulin SIZE 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg PRICE $90 $245 $90 $245 $90 $245 $90 $245 $90 $245 $90 CAT. # Y1061 Y1070 Y1071 Y1080 Y1081 Y1090 Y1091 Y1100 Y1101 Y2010 Y2011 PRODUCT NAME Anti-beta-Tubulin Anti-HA Anti-HA Anti-V5 Anti-V5 Anti-Myc Anti-Myc Anti-DYKDDDDK (FLAG) Anti-DYKDDDDK (FLAG) Anti-20S Proteasome Anti-20S Proteasome SIZE 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg 25 µg 100 µg PRICE $245 $90 $245 $90 $245 $90 $245 $90 $245 $90 $245 SIZE 1 Kit 1 Kit 1 kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit 1 Kit PRICE $980 $495 $380 $390 $520 $450 Inquiry $350 $350 $300 $275 $275 $275 $425 $275 $340 $340 $340 $325 $425 $525 $780 $195 $595 $345 $360 $320 $360 $360 $360 $360 $285 $520 Kits CAT. # J1100 J1200 J2000 J2100 J2200 J2300 J2400 J2500 J2510 J2520 J3110 J3120 J3130 J3140 J3150 J3200 J3210 J3220 J4110 J4120 J4210 J4220 J4310 J4320 J4330 J4410 J4420 J4430 J4440 J4450 J4460 J5110 J5200 PRODUCT NAME E2 Screening Kit Customized E2 Screening Kit Di-ubiquitin Explorer Kit K48-linked Ub Chain Kit K63-linked Ub Chain Kit Mixed Ub Chain Kit Customized Ub Chain Kit K48 polyUb Chain-binding Protein Identification / Validation Kit K63 polyUb Chain-binding Protein Identification / Validation Kit Linear polyUb Chain-binding Protein Identification / Validation Kit SUMO1 Conjugation Kit SUMO2 Conjugation Kit SUMO3 Conjugation Kit SUMO1, SUMO2, SUMO3 Conjugation Kit Nedd8 Conjugation Kit Ubiquitin Linkage Screening Kit I Ubiquitin Linkage Screening Kit II Ubiquitin Linkage Screening Kit III Proteasome Activity Fluorometric Assay Kit I Proteasome Activity Fluorometric Assay Kit II Proteasome Inhibitor Screening Kit I Proteasome Inhibitor Screening Kit II Rapid 26S Proteasome Purification Kit-S Rapid 26S Proteasome Purification Kit-L 26S Proteasome-mediated Protein Degradation Kit PolySUMO Capture & Release Kit Unconjugated (Free) Polyubiquitin Chain Capture Kit K48 Polyubiquitin Chain Capture Kit K63 Polyubiquitin Chain Capture Kit Linear Polyubiquitin Chain Capture Kit Polyubiquitin Chain Capture Kit Misfolded Protein Ubiquitination Kit Loading Control Antibody Kit www.ubpbio.com HIGH QUALITY REAGENTS AND SERVICES, ACCELERATED DISCOVERIES Protein Expression / Purification Reagents CAT. # P1010-10 P1010-25 P1010-100 P3010-5 P3010-25 P3010-100 P3060-10 P3060-100 P3070-2 PRODUCT NAME IPTG IPTG IPTG Nickel XPure Agarose Nickel XPure Agarose Nickel XPure Agarose Glutathione Agarose Glutathione Agarose Streptavidin XPure Agarose SIZE 10 g 25 g 100 g 5 ml 25 ml 100 ml 10 ml 100 ml 2 ml PRICE $50 $100 $300 $50 $200 $500 $120 $900 $150 CAT. # P3070-5 P5010-5 P5010-25 P5040-1 P5040-5 P5040-25 P9010 P9020 P9030 PRODUCT NAME Streptavidin XPure Agarose Protein A Agarose Protein A Agarose Protein G XPure Agarose Resin Protein G XPure Agarose Resin G XPure Agarose Protein Resin Centrifuge Column Plastic cCOLUMN Plastic 1ML Column Plastic 5ML Column SIZE 5 ml 5 ml 25 ml 1 ml 5 ml 25 ml 25/Pack 5/Pack 5/Pack PRICE $350 $200 $850 $90 $285 $995 $90 $40 $50 SIZE 500 µl 6X500 µl 500 µl 6X500 µl 500 µl 6X500 µl 500 µl 6X500 µl PRICE $42 $214 $32 $163 $108 $540 $120 $595 DNA / Protein Ladders CAT. # L1010 L1011 L1020 L1021 L2010 L2011 L2020 L2021 PRODUCT NAME 100 bp DNA Ladder 100 bp DNA Ladder 1 Kb DNA Ladder 1 Kb DNA Ladder M-XStable Prestained Protein Ladder M-XStable Prestained Protein Ladder H-XStable Prestained Protein Ladder H-XStable Prestained Protein Ladder DNA / Protein Ladders Stock-to-Save Program Lowest price in market for superior quality DNA and protein ladders No shipping charge for addresses inside US and Canada Choose your combination of four products, a minimal order of $1,200 is required Orders are processed by Email or Fax only. Email: [email protected]; Fax: 1-800-682-6801 Download the order form at www.ubpbio.com/index.php/product/dna-protein-ladders.html 100 bp DNA Ladder 1 Kb DNA Ladder L1010 500 µl $29 L1020 500 µl $22 M-XStable Prestained Protein Ladder L2010 500 µl $84 www.ubpbio.com H-XStable Prestained Protein Ladder L2020 500 µl $90 DISTRIBUTORS BELGIUM, NETHERLANDS AND LUXEMBOURG CHINA Bio-Connect Services Begonialaan 3a, 6851 TE Huissen The Netherlands Tel: +31 (0)26 326 4450, +32 (0)2 503 0348 (From Belgium) Fax: +31 (0)26 326 4451, +32 (0)2 503 0327 (From Belgium) E-Mail: [email protected], [email protected] Web: http://www.bio-connectservices.com Beijing Biocoen Biotechnology Co,LTD Building 3, A301 Zhongli Tech Park, No.1,Shangdi street 3 Beijing,China ,100085 Tel: +86-10-82815185, 4000518158 Fax: +86-10-62973121 E-Mail: [email protected] Web: http://www.biocoen.com FRANCE GERMANY EUROMEDEX 24, rue des Tuileries BP 684 67460 Souffelweyersheim Cedex Tel: +33(0)3 88 18 07 22 Fax: +33(0)3 88 18 07 25 E-Mail:[email protected] Web: http://www.euromedex.com MoBiTec GmbH Lotzestrasse 22a 37083 Göttingen, Germany Tel: +49 -551-707-220, +49-551-707-2210 (Sales) Fax: +49-551-707-2222 E-Mail: [email protected] Web: http://www.mobitec.com INDIA ISRAEL GAVO No.27, 4S Square, Thanthai Periyar Nagar G.V.Resdiency Phase II, Sowripalayam Coimbatore-641 028, Tamilnadu, India Tel: +91-94422-00732, 82200-01731 E-Mail: [email protected] Web: http://www.gavohealthcare.com BioTAG P.O. Box 4067 Israel 40300 Tel: +052-5522748 Fax: +09-8989665 E-Mail: [email protected] Web: http://www.biotag.co.il JAPAN KOREA Iwai Chemicals Company Ltd. Strategic Sales and Marketing Department 2-10, 3-chome, Nihonbashi-Honcho, Chou-Ku Tokyo 103-0023, JAPAN Tel: 81-3-3241-2570 Fax: 81-3-3270-2960 E-Mail: [email protected] Web: http://www.iwai-chem.co.jp Bioclone Corp 903, 9F Hanwha bizmetro A-dong, 551-17 YangcheonRo Gangseo-Gu, Seoul, KOREA Tel: +82-2-2690-0058, +82-2-2690-0396 (Sales) Fax: +82-2-2690-0397 E-Mail: [email protected] Web: http://www.bioclone.co.kr TAIWAN UNITED KINGDOM FoliBio Technology Co., Ltd. 5F, No. 200, Sec. 3, Nanjing East Road Taipei 104, Taiwan Tel: +886-2-2731-9370 Fax: +886-2-2731-9371 E-Mail: [email protected] Web: http://www.folibio.com.tw Caltag-Medsystems Ltd. Whiteleaf Business Centre, 11, Little Balmer Buckingham, MK18 1TF, United Kingdom Tel: +44 (0)1280 827460 Fax: +44 (0)1280 827466 E-Mail: [email protected] Web: http://www.caltagmedsystems.co.uk We can deliver our products to most countries via FedEx, please send us a quotation request for your interested products. www.ubpbio.com
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