Cell, Volume 138 Supplemental Data The Sequential Action of miR156 and miR172 Regulates Developmental Timing in Arabidopsis Gang Wu, Mee Yeon Park, Susan R. Conway, Jia-Wei Wang, Detlef Weigel, and R. Scott Poethig Table S1: Primer sequence (from 5’ to 3’, F: forward; R: reverse) Name 35S::TOE1-F 35::TOE1-R 35S::miR172b-F Sequence (5’ to 3’) CGGAATTCATGTTGGATCTTAACCTCAAC GCGGTACCTGAAAAAATTGATCATTGTA CGGGGTACCTCAAGTCAAGATCAAAGGCAA 35S::miR172b-R rSPL9-F rSPL9-R rSPL10-F rSPL10-R AGTCTTGTGTGCACCCATTT TGCGCACTGTCACTACTCTCTAATCCACATCAACCACATGA AGAGAGTAGTGACAGTGCGCAGTTTGAGTCGCCAATTCCCT AGCGCACTGTCACTACTCTCTACTACTTCGGATTCACAAGG AGAGAGTAGTGACAGTGCGCTGTAGAAATCATGAGATTCAT GR-F GR-R GR-rSPL9-F GF-rSPL9-R Gate-rSPL9-F Gate-rSPL9-R ATGGAAGCTCGAAAAACAAAGAAAAAAATCAAAGGG GGGGTACC CATGGACCTGATAGCGGCGTT TTTTTGATGAAACAGAAGCT GGGGTACCATGGAGATGGGTTCCAACTC GCTCTAGATCAGAGAGACCAGTTGGTAT CACCATCACATTTGAGCGTCCAAAGATATGGTCTCTGC GAGAGACCAGTTGGTATGGTGAGAAG 3XFLAG-F ATGGACTACAAGGACCACGACGGCGACTACAAGGACCACGACATCGAC TACAAGGATGACGATGACAAGATGGAGATGGGTTCCAACTCGGGTC 3XFLAG-R SPL9-F SPL9-R Gate-rSPL10-F Gate-rSPL10-R miR172b-F miR172b-R miR156a-F miR156a-R SPL3-F TCGTGGTCCTTGTAGTCCATGTTGGTTTCCTCTTACTCAG CACCAACACATGAAGTTGGAATGAGAGATTGGG CATGCCATGGTTACAAGAATCATACGGAAATTCATGTGGGC CACCTATTCAGTTTTGGTAAGTTTTTG GATGAAATGACTAGGGAAAGT TTTCTCAAGCTTTAGGTATTTGTAG TCGGCGGATCCATGGAAGAAAGCTC CTCTCCCTCCCTCTCTTTGATTC AGGCCAAAGAGATCAGCACCGG CTTAGCTGGACACAACGAGAGAAGGC Purpose 35S::TOE1 construct 35S::miR172b construct Mutation in miR156 binding sequence in SPL9 Mutation in miR156 binding sequence in SPL10 35S::GR-rSPL9 construct pSPL9::rSPL9-cMyc and pSPL9::rSPL9-FLAG pSPL9::3XFLAG-rSPL9 pSPL10::rSPL10-cMyc and pSPL9::rSPL9-FLAG miR172b qRT-PCR miR156a qRT-PCR SPL3 qRT-PCR SPL3-R SPL4-F SPL4-R SPL5-F SPL5-R TOE1-F TOE1-R TOE2-F TOE2-R eIF4A1-F eIF4A1-R S1-F S1-R S2-F S2-R S3-F S3-R eIF4A1-ChIP-F eIF4A1-ChIP-R SPL3-T7 SPL3-SP6 SPL9-T7 SPL9-SP6 GAGAAACAGACAGAGACACAGAGGA TCAAGGGTAGAGATGACACTTCCTATGC TCTCTCATCATAGCAAGTGATGGACCCTG CCAGACTCAAGAAAGAAACAGGGTAGACAG TCCGTGTAGGATTTAATACCATGACC CGAGTTATAATAATCCCGCCGAG TTAAGGGTGTGGATAAAAGT ATGGAGAACCACATGGCTGC GGTGCTGTAGCTGCTACGGC AAACTCAATGAAGTACTTGAGGGAC TCTCAAAACCATAAGCATAAATACCC GTGGGATCCATGAGAATGAAAT CCACACTTTCACCTGAAGATCA TCTTCTTCACTTGCACCTCTCA CTGTCGATCCAAAGTCTCACAC TTCCTCATATCCCACTGCTTTT AACGACAGATGAGCTTTCTTCC CACGCCCTGGAGTTCCAACAAC GCAAATTGAGAAGGTCATGAGG CAAGCTTAATACGACTCACTATAGGGAGAAACAGACAGAGACACAGAGGA CTCTAGATTTAGGTGACACTATAGAAGCTGTACACAACGAGAGAAGGCGG CAAGCTTAATACGACTCACTATAGGGAGATAAAACAACAAATCAAAATCA CTCTAGATTTAGGTGACACTATAGAAGTGGGTATTCAAGGACAATGATAA SPL4 qRT-PCR SPL5 qRT-PCR TOE1 qRT-PCR TOE2 qRT-PCR eIF4A qRT-PCR miR172b ChIP S1 site miR172b ChIP S2 site miR172b ChIP S3 site miR172b ChIP control miR172b ChIP control SPL3 in situ hybridization SPL9 in situ hybridization
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