Curriculum Vitae Dr. R. M. Udayangani Ratnayake, PhD BSc(Hons) (Colombo), MAgr (Japan), PhD (Japan) Senior Lecturer Department of Biochemistry Faculty of Medicine & Allied Sciences Rajarata University of Sri Lanka Saliyapura, Sri Lanka Phone: +94(0)25-2234461 (Office) Mobile: +94(0)71-8387014 E-mail: [email protected] LinkdIn : http://lk.linkedin.com/pub/udayangani-ratnayake/8/1a6/21b Researchgate: http://www.researchgate.net/profile/RM_Ratnayake PERSONAL: Full Name Date of Birth Nationality Gender Status Languages : Ratnayake Mudiyanselage Udayangani Ratnayake : 4th April 1976 : Sri Lankan : Female : Single : English & Sinhala (Fluent), Japanese (usage) EDUCATION: University Education Certificate in Medical Education, Staff Development Programme, Faculty of Medicine, University of Peradeniya, Kandy, Sri Lanka. 22nd Feb. 2013 PhD in Biochemistry & Molecular Biology, Ehime University, Japan under the Monbukagukusho (MEXT) Japanese Government Fellowship. 24th Sep. 2008 M.Agr. in Biochemistry (elective courses in Cell Physiology, Molecular Genetics, Applied Molecular Biology, and Proteomics – All Distinctions - Two year fulltime research with Thesis), Faculty of Agriculture, Ehime University, Japan under Monbukagakushop fellowship (MEXT) for Asia Africa & Pacific rim countries (AAP). 30th Sep. 2005 BSc (Hons), Biological Sciences; Botany Special with Zoology & Chemistry as subsidiary subjects, Second Class Honours Upper Division with Gold Medal (Prof. B.A. Abewickkrama Award), Faculty of Science, University of Colombo, Sri Lanka. 1st Dec. 2001 1 Undergraduate subject details: Chemistry: Organic Chemistry, Inorganic Chemistry, Physical Chemistry, Electrochemistry, Analytical Chemistry, Kinetics & Enzymology, Thermodynamics & Biochemistry ( 2yrs) (Distinctions) Human Biology: Clinical Biochemistry, Physiology, Anatomy, Clinical Genetics, Histology, Embryology, Nervous system, Reproductive system, Cardio vascular System, Endocrinology, Respiratory system, Digestion, Metabolism & GIT, Parasitology, Immunology, Pathology, (-2yrs) Kingdoms of Animal comparative studies: Systems Evolution Amoeba to Primates, Man & the Environment, Ecology (Modules -2yrs) Microbiology: General & Advance (4yrs) - (Distinctions) Plant Sciences: Pant Systematics & Taxonomy, Biodiversity, Environmental Science & management, Plant Physiology, Plant Pathology, Floriculture, Plant Molecular Biology & Genetic Engineering, Plant Biochemistry, Nutrition, Food preservation & Post-harvest management, Tissue Culture & Biotechnology (4yrs modules) Genetics, Cell Biology (Distinctions) Biostatistics & Research Methodologies 3rd year Assignment-Literature Survey: Use of organic matter for Agriculture(Distinction) rd 3 year Taxonomy Field Project (Distinction) 4th year Research Project & Thesis title: ‘Flavonoid & Molecular Markers for Cryptocoryne species in Sri Lanka’ –at Department of Biochemistry & Molecular Biology, Faculty of Medicine (Distinction) Secondary Education: Visakha Vidyalaya, Colombo 4, Sri Lanka (1988-96): General Certificate of Education -Advanced Level Examination -1995 (held by the Ministry of Education, Sri Lanka) Aggregate: 284 Chemistry Botany : Distinction - (A) : Distinction - (A) Zoology Physics : Very Good Pass - (B) : Very Good Pass - (B) General Certificate of Education - Ordinary Level Examination -1992 (held by the Ministry of Education, Sri Lanka): Distinctions for All the subjects Science Mathematics English Language Sinhala Language Buddhism Eastern Music Social Sciences & History Commerce & Accounts : Distinction : Distinction : Distinction : Distinction : Distinction : Distinction : Distinction : Distinction 2 Primary Education: Kottawa Dharmapala Vidyalaya Grade 5 Scholarship examination – held by the Ministry of Education Sri Lanka Marks: 178/200 (11th place in Colombo district, 65th place in Sri Lanka) Other: Diploma in Information Technology- ITMIN Business and Management Limited (2003) – Distinction Training (Pvt) OTHER PROFESSIONAL TRAININGS: 1) Training workshop on “Toxicology testing for industrial purposes and possible alternatives” organized by Sri Lanka Association for Laboratory Animal Sciences, held at Medical Research Institute, Colombo on 14th & 15th March 2014. 2) “Conflict resolution & Good Clinical Practices” training workshop organized by FERCSLSri Lanka Medical Association, Colombo -28th Feb. 2014. 3) Inaugural Scientific Conference on “Advancement in prevention & treatment of diseases through research on animal model & alternative models” & Post congress workshop on “Alternatives” organized by Sri Lanka Association for Laboratory Animal Sciences, 25th & 26th Jan 2014 – University of Colombo. 4) Ethnic Harmony & Social Cohesion – development of course syllabus for undergraduates national workshop conducted by Faculty of Humanities, Rajarata University, 28th-29th Jan. 2014. 5) Clinical Immunology training & Young Immunologists Forum, WISC 2012, World Allergy Organization Scientific Conference and workshop, Hyderabad, India, 6th -10th December 2012. 6) International training course & Participation as a SIDCER surveyor –“International Course on Surveying & Evaluating Ethical Review Practices” conducted by the Forum for Ethical Review Committee in the Asian & Western Pacific Region (FERCAP) from 30th June to 3rd July 2012 in Colombo, Sri Lanka. 7) Special workshop for Ethical Committee members and Clinical Trial researchers- full day workshop- Sri Lanka Medical Association, 4th July 2012, BMICH, Colombo. 8) Pre-congress Seminar-Workshop and Sessions of Anuradhapura Clinical Society-2012 organized with SLMA-FERCSL-ERC/FMAS/RUSL -17th Oct. 2012- Session Chair, organization & participation. 9) FERCAP Precongress & Conference-2012, Waters’ Edge, Colombo- 18th-21st Nov. 2012. 10) Student Councillor Training Programme –28th & 29th Nov. 2011- training conducted by Staff Development Centre RUSL & Career Guidance Unit, University of Peradeniya. 11) ‘Bio-Safety: Status Verification and Risk Assessment at National Level’ – workshop organized by Ministry of Environment and Natural Resources & Sri Lanka council for Research Policy. Held in Colombo. 24th -26th Nov. 2009. 12) 11th International Union of Biochemistry and Molecular Biology ( IUBMB), Kyoto, Japan (2006) 13) International Conference on “Organelle Protein”, Osaka, Japan (2005) 3 14) 1-3rd Matsuyama International Symposium on Cell-Free Sciences-Protein Island Matsuyama (PIM), Matsuyama, Japan (2005, 2006, 2007) PROFESSIONAL APPOINTMENTS AT UNIVERSITIES - (12 YEARS POST GRADUTE SERVICE): Senior Lecturer Grade 11: Department of Biochemistry, Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka (June 2011 –to date) Temporary Senior Lecturer Grade 11 (Full time): For Biochemistry, Molecular Biology, Chemical Biology, Pharmaceutical & Microbial Biochemistry, Department of Chemistry, Faculty of Science, University of Colombo (Feb. 2009- Oct. 2010) Visiting Lecturer in Biochemistry: Module – Tools in Biochemistry, Department of Chemistry, Faculty of Science, University of Kelaniya, Sri Lanka (2009) Post Graduate (Masters & Doctoral degree) Researcher – Monbukagakusho- Japanese Government Scholar, Ehime University, Matsuyama, Japan (Sep. 2003- Oct. 2008) Post Graduate Researcher – Medicinal Plants- Biochemical properties -Faculty of Medicine & Science, University of Colombo (Dec. 2001- Sep 2003) Temporary Demonstrator (Full time) – Biochemistry, Genetics & Microbiology (in charge) Department of Botany, University of Colombo (Dec. 2001- 2003) RESEARCH & PUBLICATIONS: Thesis: 1) R.M.U. Ratnayake, Characterization of chloroplast molecular chaperones; Hsp70s & Hsp90. Ph.D Thesis, United Graduate School. Ehime University, Matsuyama, Japan (2008) 2) R.M.U. Ratnayake, Characterization of chloroplastic Hsp70s in higher plants, Master’s Degree Thesis, Faculty of Agriculture, Ehime University, Japan (2005) 3) R.M.U. Ratnayake, Flavonoid and Molecular Markers for Cryptocoryne Species in Sri Lanka, BSc Sp. Degree Thesis, Faculty of Science, University of Colombo, Sri Lanka (2001) International publications: 1) R.M.U. Ratnayake, H. Inoue, H. Nonami and M. Akita, Alternative processing of Arabidopsis Hsp70 precursors during protein import into chloroplasts. Biosci. Biotechnol. Biochem., 72(11), 2926-2935 (2008) 4 2) H. Inoue, R.M.U. Ratnayake, H. Nonami and M. Akita, Development and optimization of an in vitro chloroplastic protein import assay using recombinant proteins. Plant Physiol. Biochem., 46(5-6), 541-549 (2008) 3) R.M.U. Ratnayake, Y. Kakinuma and M. Akita, Characterization of chloroplast Hsp90 homologue. The 2nd International Conference on Bioinformatics and Biomedical Engineering, ISBN: 978-1-4244-1747-6, 1, 144-147 (2008) 4) Y.S.G. Wimalasiri, R.M.U. Ratnayake*, T.D.N. Karunaratne & K.K.D.S. Ranaweera, Study of Prakriti - body constituents and temperament: its relationship with the development of food hypersensitivity; a common non communicable disease in Sri Lanka, Abstract & Oral Presentation. 1st International conference in Unani, Siddha, Ayurveda and Traditional Medicine, 19-21 Dec. 2013, Institute of Indigenous Medicine, University of Colombo, Sri Lanka. 5) Y.S.G. Wimalasiri, R.M.U. Ratnayake*, T.D.N. Karunaratne & K.K.D.S. Ranaweera, Identification of Specific Foods Causing Hypersensitivity among School Children in Colombo District- A Pilot Study, International Conference on Public Health Innovations. Abstract & Poster presentations. 2nd- 4th 2013, National Institute of Health Sciences, Kalutara, Sri Lanka. 6) Y.S.G. Wimalasiri, R.M.U. Ratnayake, T.D.N. Karunaratne & K.K.D.S. Ranaweera, Identification of Foods Causing Hypersensitivity/Allergy Among School Children in Two Sub-Urban Schools in Colombo District, Sri Lanka, WISC 2012, World Allergy Organization Scientific Conference, 6th -10th December 2012, Hyderabad, India. 7) R.M.U. Ratnayake*, H. Nonami and M. Akita, Characterization of chloroplast Hsp70s. B3-17, 32nd Federation of European Biochemical Societies Congress on Molecular Machines, July 7-12, 2007 (FEBS2007), Vienna, Austria 8) Mitsuru Akita, Hitoshi Inoue, Udayangani Ratnayake. M. U., Shuurou Hirano, Hiroki Fujisawa and Aki Nakaji, In Vitro Chloroplastic Protein Import Experiments with Recombinant Precursor Proteins. Abstract & Poster Presentation. Plant and Cell Physiology Supplement, vol. 2007, pp.832-832 (2007) National 9) K.J. Wijesinghe, R.M.U. Ratnayake* & N.V. Chandrasekaran, Use of Chelax-100 for a Rapid Extraction of DNA for PCR based Sexing of Domestic Chicken, Extended Abstract & Oral Presentation, RUSL-2013 Symposium, 7th Feb. 2013, Rajarata University of Sri Lanka. 10)A.N.I. Perumal, R.M.U. Ratnayake* & N.V. Chandrasekaran, Construction of recombinant E. coli signal peptidase 1 for in vitro organelle protein processing studies. Proceedings of Sri Lanka Association for the Advancement of Science- Section D, Extended Abstract & Oral Presentation on 12th Dec. 2012, 68th Annual Session (2012) 11) R.M.U. Ratnayake, T.N. Pieris, G.I. Seneviratne, T.L.S .Tirimanne and E.H. Karunanayake, Flavonoid and molecular markers for Cryptocoryne species in Sri Lanka. Extended Absract. Proceedings of Sri Lanka Association for the Advancement of Science58th Annual Session/Section D. 2002. Research Selection for Oral Presentations: 5 1) 2) 3) 4) 5) Topic: Study of Prakriti - body constituents and temperament: its relationship with the development of food hypersensitivity; a common non communicable disease in Sri Lanka 1st International conference in Unani, Siddha, Ayurveda and Traditional Medicine held at Institute of Indigenous Medicine, University of Colombo, Rajagiriya, Colombo (Dec. 2013)) Topic: Use of Chelax-100 for a Rapid Extraction of DNA for PCR based Sexing of Domestic Chicken. 3rd RUSL symposium held at Faculty of Agriculture, Rajarata University, Pulliyankulama, Anuradhapura, Sri Lanka (Feb. 2013) Topic: Construction of recombinant E. coli signal peptidase 1 for in vitro organelle protein processing studies, Sri Lanka Association of Advancement of Science, Section D, held at University of Colombo, Colombo, Sri Lanka (Dec. 2012) Topic: Characterization of chloroplast Hsp90 homologue. The 2nd International Conference on Bioinformatics and Biomedical Engineering, ICBBE 2008, Venue: Shanghai, China ( May 2008) Topic: Flavonoid and molecular markers for Cryptocoryne species in Sri Lanka. SLAAS (58th Annual Session) / Section D, Venue: University of Colombo, Colombo, Sri Lanka (2002) AWARDS, GRANTS & SCHOLARSHIPS: 1) Travel grant – From Ehime University, Japan for promoting institute’s research at Federation of European Biochemical Societies (FEBS) conference in Vienna, Austria 2007 2) Monbukagakusho (Japanese Government –MEXT Scholarship) fellowship for Doctoral Studies at United Graduate School, Ehime, University Japan (Oct. 2005Sep.2008) 3) Recipient of Japanese Government (Monbukagakusho) Full Scholarship awarded for Asian African and Pacific rim countries (AAP Program) for Master’s degree studies at Faculty of Agriculture, Ehime University, Japan(Oct. 2003-Sep. 2005) 4) Gold Medal - Professor B. A. Abewickrama Award for Excellence, University of Colombo, Convocation 2002 5) Merit Scholarship - Ministry of Higher Education, Sri Lanka for attaining highest aggregate (Country-Best Results) among students in Biological Science stream in G.C.E. A/L examination, 1995. 6) School Prize - Distinctions for all the Eight Subjects in G.C.E. O/L Examination-Visakha Vidyalaya Prize Giving Ceremony-1992 7) Scholarship - Grade 5 Scholarship Examination, Ministry of Education, Sri Lanka -1987(Country Rank-65th, Colombo District Rank-11th) PROFESSIONAL SERVICES: Professional Services in National Level: Treasurer, Sri Lanka Association for Laboratory Animal Science (SLALAS) - Jan. 2014 onwards) 6 Council member and Founder member of College of Biochemists, Sri Lanka (Since 2013) National Coordinator for Fish Processing Program in Sri Lanka– Facilitated by Ehime Prefectural Government of Japan (2014 onwards) Executive Committee member of Japanese Graduates Alumni Association of Sri Lanka (JAGAAS) – (May 2013 to date) Management Committee Member - Forum of Ethics Review Committees of Sri Lanka (FERCSL) – Sri Lanka Medical Association – (2012 onwards) Rajarata University Coordinator for Japan - Sri Lanka Symposium organized by International Division- University of Peradeniya (2013) Chief Organizer, Medical Faculty Stall under RUSL-UGC, Dayata Kirula Exhibition, Oya Maduwa, Anuradhapura (2012) Professional Services at Rajarata University: Chief coordinator for Fish Processing Program Induction at Rajarata University & coordination with Ehime Prefecture government & Faculties of Applied Sciences, Agriculture & Medicine (from 2014) Panel member & Faculty representative for Social Cohesion program (from Jan 2014) Representation of VC for Japanese education institute introduction program held at Japanese Embassy & delivering main presentation about RUSL at Galadari Hotel, July 2013 Senate member (Faculty Representative), Rajarata University of Sri Lanka (Aug. 2012 onwards) Representative (2012-2013) & Member of the management committee (from 2012) to the Forum of Ethics Review Committee Sri Lanka (FERCSL) Founder Secretary (Oct. 2011- Oct. 2013) & Member (Oct. 2011 to the date) of Ethics Review Committee of Rajarata University Medical Faculty jointly with Anuradhapura Clinical Society (ERC,FMAS,RUSL) Secretary (Oct. 2011 –Oct 2013) & Member (to the date) of Research & Higher Degrees Committee (REHDC), Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka Member of Curriculum Development Committee (CDC), Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka (Oct. 2011 to the date) Member of the Student Discipline Committee, Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka (Oct. 2011 to date) Member of Food & Canteen Committee, Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka ( Oct. 2011 to the date) Founder Executive Committee Member of Medical Teachers’ Union, Faculty of Medicine & Allied Sciences, Rajarata University of Sri Lanka (Aug. 2011 to the date) Panel Judge/ Reviewer- Medicine - Rajarata University Symposium held at the Faculty of Applied Sciences, March 2012 Panel Judge/ Reviewer – Medicine- Rajarata University Symposium held at Faculty of Agriculture, RUSL, Feb 2013 Chief Coordinator – Medical Exhibition-Dayata Kirula National Exhibition 2012 organized by the RUSL, University Grant Commission (Won 5th place) 7 Reviewer & Session co-chair 1st National Conference in Archaeology, Organized by Department of Humanities, Rajarata University of Sri Lanka, 18th Dec. 2013. Inter-faculty teaching: Animal & Human Food Nutrition program, Faculty of Agriculture Guest Lecture – Recombinant DNA technology for Food quality improvement (2014) Assignment -Animal based food – nutrition values and health aspects (Feb – May 2014) Research supervision -2 projects (Jan –Aug.2014) At Department of Biochemistry, FMAS: Seminar Coordinator (2011 & 2012) Tutorial Coordinator (2011 & 2012) Lecturing, Designing & conducting practical & Examination MBBS Preclinical years (2011 on wards) Professional Services at University of Colombo: Secretary (2001-2002) & Executive member (2000-2001)Botanical Society (BotSoc), Department of Botany Biochemistry Seminar academic coordinator (2009-2010), Department of Chemistry Coordination of Proteomics course presentations (2009-2010), Department of Chemistry Lectures, Practicals & Examination : Introduction to metabolism, Advance Biochemistry, Proteomics, Pharmaceutical Biochemistry, Microbial Biochemistry (2009-2011) Research & thesis supervision : Seven final year undergraduate thesis (2009-2013) RESEARCH SUPERVISION: 1. MPhil/PhD candidate, Faculty of Graduate Studies, University of Colombo – Dr. YSG Wimalasiri, Probationary Lecturer, Institute of Indigenous Medicine, University of Colombo - Thesis advisor and main research Supervisor under HETC project (2012-2015) 2. BSc. Agr. Specials Projects in Food Science & Nutrition candidates; a. Evaluation of Glycemic Index and Nutrient values of Thriposha & Similar products (2014). b. Assessment of dietary patterns of adolescents in schools of Anuradhapura municipal council area (2014). c. Evaluation of life style, health and nutritional status of adolescents in schools of Anuradhapura Municipal Council area (2014). 3. BSc (Hons) in Biochemistry & Molecular Biology candidates of Department of Chemistry, University of Colombo – Main Research supervision & thesis advisor: 8 a. Amplification and Analysis of Microsatellite loci for Sex Determination of Birds (20092010) b. Characterization of organelle Signal Peptidase Type 1; construction of recombinant E. coli. Leader peptidase & protein assay (2009-2010) c. Development of a DNA Test method to Sex Sri Lankan Magpie(2009-2010) d. Amplified Fragment Length Polymorphism (AFLP) analysis of Sri Lankan Magpie for true Paternity and Relatedness determination(2009-2010) e. Cloning and characterization of E. coli Hsp90 homologue; HtpG (2009-2010) f. Expression of a recombinant E. coli Leader peptidase and protein assays (2012-2013) g. Testing of a developed DNA sex determination method (2012-13) SPECIAL SKILLS: Laboratory Management & Supervision: Biochemistry & Molecular biology, Genetics Laboratories, Advance Microbiology Labs / Biotechnology / Tissue Culture Laboratories & Basic Chemistry Laboratory management Good Microbial practices Clinical Biochemistry labs: Blood analysis, Urine analysis, Lipid Profiling, Protein analysis, tissue staining, blood pictures Advance Molecular Biology techniques: Expertise: rDNA technology, DNA sequencing, Primer designing, PCR, cDNA, Molecular cloning, Mapping, protein expression & purification, SDS-PAGE, Native PAGE, bloting methods, Insilco studies; Bioinformatics tools, DNA finger printing methods Chromatography techniques ELISA, Immunology protocols Tissue culture, Cell culture Teaching & Research Interests: Molecular Medicine, Clinical Biochemistry/ Clinical Chemistry Molecular Biology: Proteomics, Genomics, & Clinical Molecular Biology, Molecular Genetics & Molecular Physiology Cellular Biochemistry, Cell Biology & Cell Physiology, Molecular Cell Biology Biotechnology & Genetic Engineering Food & Nutrition Genetics and Molecular Taxonomy (Prokaryotic & Eukaryotic) Chemical Biology, Microbial Biochemistry & Plant Biochemistry Allergy & Immunology (Research) Pharmaceutical Biochemistry & Pharmacology (research) Nutrition – epidemiological studies (research) Medical & Science education methodologies (research) Medicinal Plants / Herbal / Ayurvedic remedies as treatment options (research) 9 ONGOING PROJECTS: Pharmacological properties of active metabolites/analysts of native medicinal herbs/plants Membrane proteins characterization Proteomics studies of pre-proteins and cellular house - keeping enzymes Nutritional values and health aspects of rice, vegetable and cereal based products Food habits, food menus and life style on growth of children and adolescents Effects of heavy metals on sub-fertility levels in Sri Lanka Epidemiology of contact & food hypersensitivity in Sri Lanka Evaluation of the efficacy of indigenous medicine treatment options for food based hypersensitivity Fish processing methods & aspects on nutrition Molecular sexing of indigenous monomorphic birds Educational methodologies and psychometric assessments of students PROFESSSIONAL MEMBERSHIPS: Forum of Ethical Review Committees of Asian Western Pacific Region- FERCAP World Allergy Organization (WAO) - Junior Members Group Indian Society of Allergy Allergy & Immunology Society of Sri Lanka Institute of Biology, Sri Lanka Sri Lanka Association for Laboratory Animal Science-SLALAS Sri Lanka Association for the Advancement of Science (SLAAS) Japanese Graduates Alumni Association of Sri Lanka -JAGAAS College of Biochemists Sri Lanka – CBSL JASSO Alumni The Biochemical Society, United Kingdom (2006-09) EXTRA CURRICULAR ACTIVITES & COMMUNITY SERVICES: Member of the School Visakha Vidyalaya, Colombo Basketball team(1987-1989) Under 13 Girls All Island - National Basketball Championship-1988 Member of School Visakha Vidyalaya Netball team (1989-1991) School Sports Meets – Weerasooriya House, Visakha Vidyalaya - 100 m X 4 Relay team, ( 3rd place; 1989-1991), Basket Ball team (3rd Place; 1989) Participated in high jump, long jump, 200 m running, shot put throw & Net Ball team (1988-1993) School Drama competition participation - Weerasooriya house drama-1994, Visaska Vidyalaya Eastern music orchestra- Violin- ‘Kala Ulela’ – 1992 & Singing welcome song Science Day, Visakha Vidyalaya- 1992 10 Committee member of the Botanical Society (1994-95), member of Sinhala Literature Society, Buddhist Society, Commerce Society and Science Society (1990 - 1992) of Visakha Vidyalaya, Colombo 5 Organizing guest lectures & fund raising activities /mug sales, post card sale as the secretary (2001-02) of the Botanical Society (BotSoc), University of Colombo Teaching International & Sri Lankan Culture and English, to the Japanese school children as a service to the Japan Student Services Organization (JASSO) (20032008) –member of JASSO Exhibitor, presenter & organizer of various cultural activities at Ehime District, JapanIyoshi International Fair (2007&8) - Sri Lankan stall, EPIC centre activities (20042007), Ehime University International centre activities Dr. R.M. Udayangani Ratnayake - CV- 2014 11
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