THE LACTOSE OPERON Edited By JONATHAN R. BECKWITH Harvard Medical School and DAVID ZIPSER Cold Spring Harbor Laboratory Cold Spring Harbor Laboratory 1970 Table of Contents Preface J. R. Beckwith and D. Zipser v The Lactose Operon ix Chapters I. Introduction F. Jacob and J. Monod II. Lac: The Genetic System 1 J. R. Beckwith 5 III. /S-galactosidase and Thiogalactoside Transacetylase /. Zabin and A. V. Fowler 27 IV. The Lactose Permease System of E. coli V. The Lactose Repressor E. P. Kennedy W. Gilbert and B. Muller-Hill VI. Transcription of-'the Lactose Operon in £. coli G. Contesse, M. Crepin, and F. Gros VII. Complementation in /3-galactosidase A. Ullmann and D. Perrin ' 49 93 111 143 VIII. Transcription Starts and Stops in the lac Operon J. H. Miller 173 xlX. Glucose Effects: Inducer Exclusion and Repression B. Magasanik 189 X. Polarity and Translational Punctuation Research Articles D. Zipser 221 ~- 1. Isolation of /J-Thiogalactoside Binding Proteins of £. coli by Specific Adsorbents S. Tomino and K. Paigen 233 2. Isoenzymes of Bacterial /3-galactosidase: Renaturation of Heavier Isoenzymes from Dissociated £. coli /S-galactosidase R. P. Erickson and E. Steers, Jr. 251 VI Table of Contents b On the Stoichiometry and Kinetics of « Complementation of I E. coli /8-galactosidase A. Ullmann and J. Monod 265 ;. Structural Studies of w-complemented /?-galactosidase of I E. coli M. E. Goldberg . 273 i, Partial Loss of Activity of Individual Molecules of Aged > /?-galactosidase A/I. B. Rotman 279 i. Antibody Mediated Activation of a'Defective /3-galactosidase (AMEF). Characteristics of Binding and Activation Processes F. Celada, R. Strom, and K. Bodlund 291 '. The Activation of Mutant yS-galactosidase by Specific Antibodies W. Messer and F.Melchers 305 |; Is Subunit Assembly a Rate-Limiting Factor on Induction? [: L. Adamson, C. Gross, and A. Novick 317 i Superrepressors of the lab Operon 325 S. Bourgeois and A. Jobe 'i Strains with the Promoter Deletion LI Synthesize an Altered I lac Repressor J. H. Miller, J. Platt, and K. Weber 343 i Deletion of Translational Start Signals in the lac Operon of £. coli L Eron, J. R. Beckwith, and F. Jacob 353 , Some Properties of Insertion Mutations in the lac Operon M. H. Malamy 359 . Cell-Free Studies on the Regulation of the lac Operon G. Zubay, D. A. Chambers, and L. C, Cheong 375 . Cyclic Adenosine Monophosphate Diesterase Activity and Catabolite Repression in £. coli D. Monard, J. Janecek, and H. V. Rickenberg 393 . On the Mechanism of Catabolite Repression G. Contesse, M. Crepin, and F. Gros 401 ,.^ . Mutants Missing a Factor Necessary for the Expression of Catabolite-Sensitive Operons in £. coli D. Schwartz and J. R. Beckwith VII 417
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