Non-pathogen scheme: distribution NP048 report

Summary of Results
External Quality Assessment of Food Microbiology
Non-Pathogen Scheme
Distribution Number:
NP048
Sample Numbers:
NP0142, NP0143, NP0144
Distribution Date:
Results Due:
Report Date:
May 2014
27 June 2014
18 July 2014
Samples prepared and QC
tested by:
Morolake Adedeji
Stephanie Foster
Thamayanthy Ramesh
Aneta Stranc
Anitha Tallam
Data Analysed by:
Heena Shah
Nita Patel
Report Compiled by:
Heena Shah
Nita Patel
Authorised by:
Heena Shah
Nita Patel
This report must not be reproduced without permission of the organisers.
Public Health England
Food and Environmental Proficiency Testing Unit (FEPTU)
61 Colindale Avenue
London
NW9 5EQ
Tel:
+44 (0)20 8327 7119
Fax:
+44 (0)20 8200 8264
Email: [email protected]
The data in FEPTU reports is confidential
Page 1 of 23
Printed at 17:29 on Friday, 18 July, 2014
If you require general information about the scheme please refer to:
Scheme Guide:
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1265637229388
For more specific information about results assessments, scoring
analysing your results for the proficiency testing samples please refer to:
Guide to Scoring and Statistics:
systems,
statistics,
and
guidance
on
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947373355
General guidance for z-scores:
Participants' enumeration results are converted into z-scores using the following formula:
Z = (x-X)
σ
x = participants' result (expressed as a log 10 value)
X = assigned value (participants' consensus median (expressed as a log 10 value))
σ = the fixed standard deviation for the examination (calculated by FEPTU)
The σ-value expresses the acceptable difference between the individual participant 's result and the participants'
consensus median. The σ-value used for calculating z-scores for all parameters in the Non-Pathogen Scheme
is 0.35. A guide to interpreting z-scores follows, although laboratories must interpret their scores in the context
of their own laboratory situation.
z = -1.99 to +1.99
z = -2 to -2.99 or +2 to +2.99
z= < -3.00 or > + 3.00
satisfactory
questionable
unsatisfactory
It is usually recommended that z-scores exceeding ±2 are investigated to establish the possible cause.
general rule, PHE recommends that all questionable and unsatisfactory results are investigated.
As a
FEPTU Quality Control: A minimum of 10 vials, selected randomly from the batch, are examined for the test
parameters indicated on the request/report form. The FEPTU results are determined using methods based on
ISO methods and are included in the 'intended results' letters which provide guidance for participants regarding
the assigned values.
Intended results letters are posted on the website immediately after every distribution ;
electronic notification of their availablilty is sent to all participants.
If you experience difficulties with any of the examinations please refer to section 17.0 of the Scheme Guide
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1265637229388
All participants are reminded that reporting an incorrect or incomplete identification of pathogen from food
samples could have serious public health implications. Similarly, the levels of mirco-organisms reported in the
sample may affect the subsequent outcome for the product .
Please contact FEPTU staff for advice and information:
Repeat samples
Carmen Gomes or Kermin Daruwalla
Data Analysis
Heena Shah or Nita Patel
Microbiological advice
Morolake Adedeji or Heena Shah
General comments and complaints
Heena Shah or Nita Patel
Scheme consultants
Melody Greenwood and Julie E.Russell
Scheme Co-ordinator
Nita Patel
Tel:
Fax:
Email:
+44 (0)20 8327 7119
+44 (0)20 8200 8264
[email protected]
Accreditation: PHE Non-pathogen EQA Scheme is accredited by the United Kingdom Accreditation Service
(UKAS) to ISO/IEC Guide 17043:2010
0006
The data in FEPTU reports is confidential
Page 2 of 23
Printed at 17:29 on Friday, 18 July, 2014
Sample: NP0142
Contents:
Pseudomonas fluorescens (10⁴) (wild type strain), Penicillium sp. (10⁴) (wild type strain), Klebsiella
aerogenes (10⁵) (wild type strain), Escherichia coli (10³) (wild type strain)
Expected Results:
All counts are expressed as log10 colony forming units (cfu) per mL reconstituted sample.
No. results
returned
Assigned value
(Participants'
median)
42
76
1.3x10⁵
0
(5.13 log₁₀)
0
4.2x10⁵
4.5x10⁵
1.6x10³
(5.62 log₁₀)
(5.65 log₁₀)
(3.2 log₁₀)
76
41
45
48
29
30
0
0
2.6x10⁴
3.1x10⁵
3.1x10⁵
6.1x10³
(4.41 log₁₀)
(5.49 log₁₀)
(5.49 log₁₀)
(3.79 log₁₀)
Colony count 22ºC
0
2.0x10⁴
(4.3 log₁₀)
Colony count 30ºC
Escherichia coli
Enterobacteriaceae
Coliforms
Moulds
Yeasts
0
Lactic acid bacteria
6.6x10⁴
(4.82 log₁₀)
Enterococci
FEPTU median
Pseudomonas spp.
Parameter
The fixed standard deviation value (σ value) used for calculation of the z-scores is 0.35 for all parameters.
4.5x10⁵
4.5x10⁵
(5.65 log₁₀)
(5.65 log₁₀)
53
23
9.2x10⁵
1.1x10⁶
(5.96 log₁₀)
(6.02 log₁₀)
Uncertainty of
assigned value
(Ux)
0.06
N/A
0.02
0.06
0.05
0.06
N/A
N/A
0.04
0.06
Standard
deviation*
0.29
N/A
0.16
0.33
0.31
0.34
N/A
N/A
0.2
0.22
3.5x10⁴ -
N/A
8.1x10³ -
6.8x10⁴ -
7.5x10⁴ -
1.3x10³ -
N/A
N/A
2.9x10⁵ -
3.3x10⁵ -
8.1x10⁴
1.4x10⁶
1.3x10⁶
3.0x10⁴
2.9x10⁶
3.3x10⁶
Expected Range
5.2x10⁵
Participants' mean
1.2x10⁵
N/A
(5.06 log₁₀)
No of outlying
counts
7
False positives
False negatives
N/A
2.5x10⁴
3.1x10⁵
3.1x10⁵
5.1x10³
(4.39 log₁₀)
(5.49 log₁₀)
(5.49 log₁₀)
(3.71 log₁₀)
8
5
6
10
7
2
0
0
0
N/A
N/A
N/A
N/A
1
3
9.1x10⁵
9.2x10⁵
(5.96 log₁₀)
(5.96 log₁₀)
6
3
0
0
4
Your result
Score for
performance
assessment
Z-score
* Robust SD based on median absolute deviation about the participant's median (MAD)
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947373355
For further information about the statistics and z-scores go to:
Total sent samples
85
Not examined
1
Non returns
1
The data in FEPTU reports is confidential
Page 3 of 23
Printed at 17:29 on Friday, 18 July, 2014
Sample: NP0143
Contents:
Pseudomonas fluorescens (10³) (wild type strain), Candida sp. (10⁴) (wild type strain), Hafnia alvei (10²)
(wild type strain), Enterococcus faecalis (10³) (wild type strain), Lactobacillus plantarum (10⁴) (wild type
strain)
Expected Results:
All counts are expressed as log10 colony forming units (cfu) per mL reconstituted sample.
FEPTU median
No. results
returned
Assigned value
(Participants'
median)
4.6x10³
4.4x10⁴
(3.66 log₁₀)
(4.64 log₁₀)
40
76
75
0
3.3x10³
8.9x10⁴
(3.52 log₁₀)
(4.95 log₁₀)
Uncertainty of
assigned value
(Ux)
0.04
0.04
Standard
deviation*
0.21
Expected Range
Participants' mean
No of outlying
counts
0
5.0x10²
(2.7 log₁₀)
40
44
47
0
5.4x10²
5.2x10²
(2.72 log₁₀)
N/A
0.06
0.05
0.28
N/A
0.29
1.0x10³ -
2.4x10⁴ -
N/A
1.0x10⁴
3.3x10⁵
3.4x10³
9.0x10⁴
(3.53 log₁₀)
(4.95 log₁₀)
7
7
N/A
N/A
3
0
Colony count 22ºC
Colony count 30ºC
Lactic acid bacteria
Enterococci
4.3x10³
2.9x10⁴
6.4x10⁴
6.3x10⁴
(3.63 log₁₀)
(4.46 log₁₀)
(4.81 log₁₀)
(4.8 log₁₀)
28
30
51
22
3.8x10³
3.1x10⁴
1.1x10⁵
1.3x10⁵
(3.58 log₁₀)
(4.49 log₁₀)
(5.04 log₁₀)
(5.11 log₁₀)
N/A
0.02
0.04
0.04
0.04
0.3
N/A
0.11
0.2
0.22
0.14
1.4x10² -
1.3x10² -
N/A
1.2x10³ -
9.7x10³ -
3.5x10⁴ -
4.1x10⁴ -
2.1x10³
2.1x10³
1.2x10⁴
9.7x10⁴
3.5x10⁵
4.1x10⁵
5.0x10²
5.1x10²
(2.7 log₁₀)
(2.71 log₁₀)
7
5
2
2
Escherichia coli
Enterobacteriaceae
Coliforms
4.7x10²
(2.67 log₁₀)
(2.73 log₁₀)
False positives
False negatives
Moulds
Yeasts
Pseudomonas spp.
Parameter
The fixed standard deviation value (σ value) used for calculation of the z-scores is 0.35 for all parameters.
N/A
N/A
3.8x10³
3.4x10⁴
1.1x10⁵
1.3x10⁵
(3.58 log₁₀)
(4.54 log₁₀)
(5.03 log₁₀)
(5.11 log₁₀)
3
5
5
6
1
0
0
0
1
1
5
Your result
Score for
performance
assessment
Z-score
* Robust SD based on median absolute deviation about the participant's median (MAD)
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947373355
For further information about the statistics and z-scores go to:
Total sent samples
85
Not examined
2
Non returns
The data in FEPTU reports is confidential
Page 4 of 23
1
Printed at 17:29 on Friday, 18 July, 2014
Sample: NP0144
Contents:
Pichia fermentans (10³) (NCPF 8835), Candida famata (10³) (wild type strain), Hafnia alvei (10⁵) (wild type
strain), Escherichia coli (10⁵) (wild type strain), Enterococcus faecium (10⁴) (wild type strain), Lactobacillus
plantarum (10⁴) (wild type strain)
Expected Results:
All counts are expressed as log10 colony forming units (cfu) per mL reconstituted sample.
FEPTU median
0
5.2x10³
0
(3.72 log₁₀)
No. results
returned
40
Assigned value
(Participants'
median)
0
Uncertainty of
assigned value
(Ux)
N/A
0.02
Standard
deviation*
N/A
Expected Range
N/A
74
73
5.3x10³
0
N/A
N/A
False positives
3
False negatives
Colony count 22ºC
Colony count 30ºC
Lactic acid bacteria
Enterococci
Escherichia coli
Enterobacteriaceae
3.6x10⁵
1.8x10⁵
2.8x10⁴
9.1x10⁴
3.8x10⁵
4.5x10⁵
(5.56 log₁₀)
(5.26 log₁₀)
(4.45 log₁₀)
(4.96 log₁₀)
(5.58 log₁₀)
(5.65 log₁₀)
39
44
47
27
30
51
20
2.6x10⁵
2.5x10⁵
1.8x10⁵
2.6x10⁴
1.8x10⁵
5.9x10⁵
5.8x10⁵
(5.4 log₁₀)
(5.27 log₁₀)
(4.41 log₁₀)
(5.26 log₁₀)
(5.77 log₁₀)
(5.77 log₁₀)
N/A
0.05
0.04
0.04
0.02
0.05
0.03
0.03
0.17
N/A
0.22
0.25
0.24
0.09
0.21
0.18
0.11
1.7x10³ -
N/A
8.2x10⁴ -
7.9x10⁴ -
5.8x10⁴ -
8.2x10³ -
5.7x10⁴ -
1.9x10⁵ -
1.8x10⁵ -
8.2x10⁵
7.9x10⁵
5.8x10⁵
8.2x10⁴
5.7x10⁵
1.9x10⁶
1.8x10⁶
5.0x10³
N/A
(3.7 log₁₀)
No of outlying
counts
2.5x10⁵
(5.4 log₁₀)
(5.41 log₁₀)
(3.72 log₁₀)
1.7x10⁴
Participants' mean
Coliforms
Moulds
Yeasts
Pseudomonas spp.
Parameter
The fixed standard deviation value (σ value) used for calculation of the z-scores is 0.35 for all parameters.
8
N/A
2.7x10⁵
2.6x10⁵
1.8x10⁵
2.5x10⁴
1.8x10⁵
5.7x10⁵
5.9x10⁵
(5.43 log₁₀)
(5.42 log₁₀)
(5.25 log₁₀)
(4.4 log₁₀)
(5.25 log₁₀)
(5.75 log₁₀)
(5.77 log₁₀)
4
6
7
4
3
5
6
0
0
0
0
0
0
0
4
1
Your result
Score for
performance
assessment
Z-score
* Robust SD based on median absolute deviation about the participant's median (MAD)
http://www.hpa.org.uk/web/HPAwebFile/HPAweb_C/1194947373355
For further information about the statistics and z-scores go to:
Total sent samples
85
Not examined
3
Non returns
The data in FEPTU reports is confidential
Page 5 of 23
1
Printed at 17:29 on Friday, 18 July, 2014
Aerobic Colony Count
(22ºC)
score
Aerobic Colony Count
(30ºC)
score
Lactic acid bacteria
score
Enterococci
score
Escherichia coli
score
Enterobacteriaceae
score
Coliform
score
Moulds
score
Yeasts
score
Presumptive Pseudomonas
spp.
score
Sample
Distribution
Performance Assessment Sheet
NP0142
NP048
NP0143
NP0144
NP0139
NP047
NP0140
NP0141
NP0136
NP046
NP0137
NP0138
Total maximum possible
score
Total percentage
Performance Assessment Comment:
Overall Performance Assessment for Distributions
NP048, NP047, NP046
Performance assessments are designed to alert participants to
on-going problems with their examinations and are provided after
every distribution. Scores are allocated to results reported for every
parameter, for every sample to help assess performance.
30
20
100
90-99
80-89
70-79
60-69
50-59
40-49
30-39
20-29
10-19
0
1-9
10
0
Your overall performance with the enumerations in the non pathogen
proficiency testing samples for the current and last two distributions is
collated in the chart to the right.
40
Number of participants
Cumulative scores are calculated for the current and previous two
distributions for the Non Pathogen Scheme.
Participants' cumulative
scores for each of the examinations are compared with the maximum
possible scores after every distribution.
50
Percentage achieved of max possible
score for stipulated distributions
Performance Assessment Comment:
Laboratories that achieve less than 70% of the maximum possible score are likely to be experiencing significant problems
with their examinations and are advised to:
a) refer to the relevant distribution reports for sample-specific comments
b) refer to the website guidance documents: www.hpa.org.uk/eqa/docs
c) contact the organisers for advice
The data in FEPTU reports is confidential
Page 6 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0142 - Presumptive Pseudomonas spp.
7.00
6.00
Participants' Median +3SD 6.01
Participants' Median +2SD 5.72
Participants' Median 5.13
5.00
Participants' Median -2SD 4.54
Log₁₀ cfu g⁻¹
Participants' Median -3SD 4.25
4.00
3.00
2.00
2099
FEPTU QC
Median
2096
2095
2094
2061
1970
1940
1803
1744
1654
1636
1609
1601
1526
1507
1502
1456
800
1427
785
784
735
700
577
506
502
497
473
421
415
325
316
279
243
226
185
166
145
18
137
1.58
Laboratory identification number
Key: reported result by method
No Method Stated
PCN (Pseudomonas
cetrimide sodium nalidixate
agar)
PCFC/CFC (Pseudomonas
CFC agar containing
cetrimide, fucidin and
cephaloridine)
Other
NP0142 - Yeasts
No data for graph
The data in FEPTU reports is confidential
Page 7 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0142 - Moulds
6.00
5.75
5.25
Participants' Median -3SD
+ 0.5 log10
4.91 4.91
4.75
Participants' Median 4.41
Log₁₀ cfu g⁻¹
4.25
Participants' Median -+0.5
0.5log10
log10 3.91
3.91
3.75
3.25
2.75
9
18
46
127
137
145
166
185
226
243
255
264
279
288
316
318
325
336
415
421
445
452
473
497
502
503
506
514
563
566
577
700
724
785
800
918
1285
1294
1427
1456
1502
1507
1526
1581
1601
1607
1621
1636
1639
1654
1655
1664
1744
1793
1803
1810
1930
1932
1940
1954
1964
1970
2019
2023
2035
2060
2061
2064
2072
2093
2094
2095
2096
2098
2099
2100
FEPTU QC
Median
2.46
Laboratory identification number
Key: reported result by method
No Method Stated
DG (Dichloran glycerol
chloramphenicol agar)
YGC (Yeast Extract
Glucose Chloramphenicol
Agar)
Other
DRBC (Dichloran Rose
Bengal Chloramphenicol
agar)
OGYEA (Oxytetracycline
glucose yeast extract agar)
NP0142 - Coliform
8.00
7.00
Participants' Median +3SD 6.47
Participants' Median +2SD 6.15
Log₁₀ cfu g⁻¹
6.00
Participants' Median 5.49
5.00
Participants' Median -2SD 4.83
Participants' Median -3SD 4.50
4.00
2098
FEPTU QC
Median
2096
2093
2072
2019
1992
1970
1940
1938
1810
1803
1783
1654
1636
1609
1601
1507
1502
1456
918
1294
800
785
784
735
506
502
452
445
415
325
318
316
288
243
226
166
127
9
18
3.41
Laboratory identification number
Key: reported result by method
No Method Stated
VRBA (Violet red bile agar)
Petrifilm
MPN - Tempo
MPN – Tube combination
Other
The data in FEPTU reports is confidential
Page 8 of 23
Chromogenic agar
Printed at 17:29 on Friday, 18 July, 2014
NP0142 - Enterobacteriaceae
8.00
7.00
Participants' Median +3SD 6.42
Participants' Median +2SD 6.11
Log₁₀ cfu g⁻¹
6.00
Participants' Median 5.49
5.00
Participants' Median -2SD 4.87
Participants' Median -3SD 4.56
4.00
2093
2095
2096
2098
2099
FEPTU QC
Median
2095
2096
2098
2099
FEPTU QC
Median
2072
2093
2019
1940
1938
1930
1803
1783
1744
1664
1654
1636
1609
1601
1507
1502
1456
1427
800
1294
785
784
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
166
18
127
3.48
Laboratory identification number
Key: reported result by method
No Method Stated
VRBGA (Violet red bile
glucose agar)
MPN – Tube combination
MPN - Tempo
Petrifilm
Chromogenic agar
Other
NP0142 - Escherichia coli
6.00
5.00
Participants' Median +3SD 4.81
Participants' Median +2SD 4.47
4.00
Log₁₀ cfu g⁻¹
Participants' Median 3.79
Participants' Median -2SD 3.10
3.00
Participants' Median -3SD 2.76
2.00
2072
2019
1992
1970
1938
1810
1783
1744
1664
1654
1636
1609
1601
1507
1502
1456
1427
1294
800
785
784
735
700
506
502
497
452
445
415
325
318
316
288
279
243
226
166
18
127
1.00
Laboratory identification number
Key: reported result by method
No Method Stated
VRBL
Direct enumeration using
membrane technique on to
TBX
Petrifilm
Direct enumeration using
membrane technique on to
TBA
Chromogenic agar
TBX spread/pour plate
MPN – Tube combination
Other
The data in FEPTU reports is confidential
Page 9 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0142 - Enterococci
No data for graph
NP0142 - Lactic acid bacteria
No data for graph
The data in FEPTU reports is confidential
Page 10 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0142 - Aerobic Colony Count (30ºC)
8.00
7.00
Participants' Median -3SD 6.56
Participants' Median + 0.5 log10 6.46
Log₁₀ cfu g⁻¹
6.00
Participants' Median 5.96
Participants' Median - 0.5 log10 5.46
Participants' Median -3SD 5.37
5.00
4.00
Laboratory identification number
2098
2099
FEPTU QC
Median
2096
2095
2093
2072
2035
2019
1992
1970
1954
1940
1938
1930
1810
1803
1793
1783
1744
1664
1654
1636
1621
1609
1601
1507
1502
1456
1427
800
1301
785
784
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
185
166
18
127
3.08
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
NP0142 - Aerobic Colony Count (22ºC)
7.00
Participants' Median -3SD 6.67
6.50
6.00
Log₁₀ cfu g⁻¹
5.50
Participants' Median + 0.5 log10 6.52
Participants' Median 6.02
Participants' Median - 0.5 log10 5.52
Participants' Median -3SD 5.37
5.00
4.50
4.00
3.50
FEPTU QC
Median
2099
2095
2093
2019
1992
1970
1810
1783
1654
1601
1507
1502
800
785
506
502
497
452
415
316
226
185
18
3.34
Laboratory identification number
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
The data in FEPTU reports is confidential
Page 11 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0143 - Presumptive Pseudomonas spp.
6.00
5.00
Log₁₀ cfu g⁻¹
4.00
Participants' Median -3SD 4.15
Participants' Median + 0.5 log10 4.02
Participants' Median 3.52
3.00
Participants' Median - 0.5 log10 3.02
Participants' Median -3SD 2.89
2.00
2099
FEPTU QC
Median
2096
2095
2094
2061
1970
1940
1803
1744
1654
1636
1609
1601
1526
1507
1502
800
1427
785
735
700
577
506
502
497
473
421
415
325
316
243
226
185
166
145
137
18
127
1.50
Laboratory identification number
Key: reported result by method
No Method Stated
PCN (Pseudomonas
cetrimide sodium nalidixate
agar)
PCFC/CFC (Pseudomonas
CFC agar containing
cetrimide, fucidin and
cephaloridine)
Other
NP0143 - Yeasts
7.00
6.50
6.00
Participants' Median +3SD 5.80
5.50
Log₁₀ cfu g⁻¹
5.00
4.50
Participants' Median +2SD 5.51
Participants' Median 4.95
Participants' Median -2SD 4.38
Participants' Median -3SD 4.10
4.00
3.50
3.00
9
18
46
127
137
145
166
185
226
243
255
264
279
288
316
318
325
336
415
421
445
452
473
497
502
503
506
514
563
566
577
700
724
785
800
918
1285
1294
1427
1502
1507
1526
1581
1601
1607
1609
1621
1636
1639
1654
1655
1664
1803
1810
1930
1932
1940
1954
1964
1970
2019
2023
2035
2060
2061
2064
2072
2093
2094
2095
2096
2098
2100
FEPTU QC
Median
2.62
Laboratory identification number
Key: reported result by method
No Method Stated
DG (Dichloran glycerol
chloramphenicol agar)
YGC (Yeast Extract
Other
Glucose Chloramphenicol
Agar)
The data in FEPTU reports is confidential
DRBC (Dichloran Rose
Bengal Chloramphenicol
agar)
Page 12 of 23
OGYEA (Oxytetracycline
glucose yeast extract agar)
Printed at 17:29 on Friday, 18 July, 2014
NP0143 - Moulds
No data for graph
NP0143 - Coliform
4.00
3.75
Participants' Median +3SD 3.60
Participants' Median +2SD 3.31
3.25
Log₁₀ cfu g⁻¹
2.75
2.25
Participants' Median 2.73
Participants' Median -2SD 2.15
Participants' Median -3SD 1.86
1.75
1.25
2098
FEPTU QC
Median
2096
2093
2072
2019
1992
1970
1940
1938
1810
1803
1783
1654
1636
1609
1601
1507
1502
1456
918
1294
785
735
700
506
502
452
445
415
325
318
316
288
243
226
166
127
9
18
0.95
Laboratory identification number
Key: reported result by method
No Method Stated
VRBA (Violet red bile agar)
Petrifilm
MPN - Tempo
MPN – Tube combination
Other
The data in FEPTU reports is confidential
Page 13 of 23
Chromogenic agar
Printed at 17:29 on Friday, 18 July, 2014
NP0143 - Enterobacteriaceae
5.00
4.50
4.00
Participants' Median +3SD 3.61
3.50
Participants' Median +2SD 3.31
3.00
Log₁₀ cfu g⁻¹
Participants' Median 2.72
2.50
Participants' Median -2SD 2.12
2.00
Participants' Median -3SD 1.82
1.50
1.00
2099
FEPTU QC
Median
2098
2096
2095
2093
2072
2019
1938
1930
1803
1664
1654
1636
1609
1601
1507
1502
1456
1427
785
1294
735
724
700
506
502
497
452
445
415
325
318
316
288
243
226
166
18
127
0.74
Laboratory identification number
Key: reported result by method
No Method Stated
MPN - Tempo
VRBGA (Violet red bile
glucose agar)
MPN – Tube combination
Petrifilm
Chromogenic agar
Other
NP0143 - Escherichia coli
No data for graph
The data in FEPTU reports is confidential
Page 14 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0143 - Enterococci
6.00
5.50
5.00
4.50
Participants' Median -3SD
+ 0.5 log10
4.08 4.08
Log₁₀ cfu g⁻¹
4.00
Participants' Median 3.58
3.50
Participants' Median -+0.5
0.5log10
log10 3.08
3.08
3.00
2.50
2099
FEPTU QC
Median
2095
2019
1940
2072
1938
1938
1803
1654
1636
1507
1502
800
785
735
700
506
502
497
452
415
325
316
288
243
226
137
127
18
2.09
Laboratory identification number
Key: reported result by method
No Method Stated
KF Streptococcus agar
S + B (Slanetz and Bartley)
Other
NP0143 - Lactic acid bacteria
6.00
5.60
5.20
Participants' Median -3SD 5.09
Participants' Median + 0.5 log10 4.99
Log₁₀ cfu g⁻¹
4.80
Participants' Median 4.49
4.40
4.00
Participants' Median - 0.5 log10 3.99
Participants' Median -3SD 3.89
3.60
FEPTU QC
Median
2099
2096
2095
1810
1803
1744
1654
1636
1609
1601
1507
1502
1427
800
735
700
502
497
452
415
325
316
288
243
226
185
137
18
3.30
Laboratory identification number
Key: reported result by method
No Method Stated
MRS (De Man, Rogosa,
Sharpe agar)
The data in FEPTU reports is confidential
Other
Page 15 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0143 - Aerobic Colony Count (30ºC)
7.00
6.50
6.00
Participants' Median -3SD 5.71
Participants' Median + 0.5 log10 5.54
Log₁₀ cfu g⁻¹
5.50
Participants' Median 5.04
5.00
Participants' Median - 0.5 log10 4.54
4.50
Participants' Median -3SD 4.37
4.00
3.50
2098
2099
FEPTU QC
Median
FEPTU QC
Median
2096
2099
2095
2093
2072
2035
2019
1992
1970
1954
1940
1938
1930
1810
1803
1793
1744
1664
1654
1636
1621
1609
1601
1507
1502
1456
1427
800
1301
785
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
185
166
18
127
3.23
Laboratory identification number
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
NP0143 - Aerobic Colony Count (22ºC)
7.00
6.50
6.00
Participants' Median -3SD
+ 0.5 log10
5.61 5.61
5.50
Log₁₀ cfu g⁻¹
Participants' Median 5.11
5.00
Participants' Median -+0.5
0.5log10
log10 4.61
4.61
4.50
4.00
3.50
2095
2093
2019
1992
1970
1810
1654
1601
1507
1502
800
785
506
502
497
452
415
316
226
185
18
3.31
Laboratory identification number
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
The data in FEPTU reports is confidential
Page 16 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0144 - Presumptive Pseudomonas spp.
No data for graph
NP0144 - Yeasts
6.00
5.00
Participants' Median -3SD
4.24 4.22
+ 0.5 log10
4.00
Log₁₀ cfu g⁻¹
Participants' Median 3.72
- 0.5 log10
Participants' Median -3SD
3.21 3.22
3.00
2.00
9
18
46
127
137
145
166
226
243
255
264
279
288
316
318
325
336
415
421
445
452
473
497
502
503
506
514
563
566
577
700
724
785
800
918
1285
1294
1427
1456
1502
1507
1526
1581
1601
1609
1621
1636
1639
1654
1655
1664
1744
1803
1810
1930
1932
1940
1954
1964
1970
2019
2023
2035
2060
2061
2064
2072
2093
2094
2095
2096
2098
2099
2100
FEPTU QC
Median
1.08
Laboratory identification number
Key: reported result by method
No Method Stated
DG (Dichloran glycerol
chloramphenicol agar)
YGC (Yeast Extract
Glucose Chloramphenicol
Agar)
Other
The data in FEPTU reports is confidential
DRBC (Dichloran Rose
Bengal Chloramphenicol
agar)
Page 17 of 23
OGYEA (Oxytetracycline
glucose yeast extract agar)
Printed at 17:29 on Friday, 18 July, 2014
NP0144 - Moulds
No data for graph
NP0144 - Coliform
7.00
Participants' Median -3SD 6.09
6.00
Participants' Median + 0.5 log10 5.91
Log₁₀ cfu g⁻¹
Participants' Median 5.41
5.00
Participants' Median - 0.5 log10 4.91
Participants' Median -3SD 4.74
2098
FEPTU QC
Median
2096
2093
2072
2019
1970
1940
1938
1810
1803
1783
1654
1636
1609
1601
1507
1502
918
1456
800
785
784
735
506
502
452
445
415
325
318
316
288
243
226
166
127
9
18
4.00
Laboratory identification number
Key: reported result by method
No Method Stated
VRBA (Violet red bile agar)
Petrifilm
MPN - Tempo
MPN – Tube combination
Other
The data in FEPTU reports is confidential
Page 18 of 23
Chromogenic agar
Printed at 17:29 on Friday, 18 July, 2014
NP0144 - Enterobacteriaceae
8.00
7.75
7.25
6.75
6.25
Participants' Median -3SD 6.13
Log₁₀ cfu g⁻¹
Participants' Median + 0.5 log10 5.90
5.75
Participants' Median 5.40
5.25
Participants' Median - 0.5 log10 4.90
4.75
Participants' Median -3SD 4.66
4.25
2096
2098
2099
FEPTU QC
Median
2098
2099
FEPTU QC
Median
2095
2096
2093
2072
2019
1940
1938
1930
1803
1783
1744
1664
1654
1636
1609
1601
1507
1502
1456
800
1427
785
784
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
166
18
127
3.88
Laboratory identification number
Key: reported result by method
No Method Stated
VRBGA (Violet red bile
glucose agar)
MPN – Tube combination
MPN - Tempo
Petrifilm
Chromogenic agar
Other
NP0144 - Escherichia coli
7.00
6.75
6.25
Participants' Median -3SD 5.97
5.75
Log₁₀ cfu g⁻¹
5.25
4.75
Participants' Median + 0.5 log10 5.77
Participants' Median 5.27
Participants' Median - 0.5 log10 4.77
Participants' Median -3SD 4.56
4.25
3.75
3.25
2095
2093
2072
2019
1992
1970
1940
1938
1810
1803
1783
1744
1664
1654
1636
1609
1601
1507
1502
1456
1427
800
785
784
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
166
18
127
2.92
Laboratory identification number
Key: reported result by method
No Method Stated
VRBL
Direct enumeration using
membrane technique on to
TBX
Petrifilm
Direct enumeration using
membrane technique on to
TBA
Chromogenic agar
TBX spread/pour plate
MPN – Tube combination
Other
The data in FEPTU reports is confidential
Page 19 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0144 - Enterococci
6.00
5.75
5.25
Participants' Median -3SD
+ 0.5 log10
4.91 4.91
4.75
Participants' Median 4.41
Log₁₀ cfu g⁻¹
4.25
Participants' Median -+0.5
0.5log10
log10 3.91
3.91
3.75
3.25
2.75
2099
FEPTU QC
Median
2095
1938
1940
2072
1803
1938
1654
1636
1507
1502
800
785
784
735
700
506
502
497
452
415
325
316
288
243
226
137
127
18
2.46
Laboratory identification number
Key: reported result by method
No Method Stated
KF Streptococcus agar
S + B (Slanetz and Bartley)
Other
NP0144 - Lactic acid bacteria
7.00
6.50
6.00
Participants' Median -3SD 5.88
Participants' Median + 0.5 log10 5.76
Log₁₀ cfu g⁻¹
5.50
Participants' Median 5.26
5.00
Participants' Median - 0.5 log10 4.76
Participants' Median -3SD 4.63
4.50
4.00
FEPTU QC
Median
2099
2096
2095
1810
1803
1783
1744
1654
1636
1609
1601
1507
1502
1427
800
735
700
502
497
452
415
325
316
288
243
226
137
18
3.81
Laboratory identification number
Key: reported result by method
No Method Stated
MRS (De Man, Rogosa,
Sharpe agar)
The data in FEPTU reports is confidential
Other
Page 20 of 23
Printed at 17:29 on Friday, 18 July, 2014
NP0144 - Aerobic Colony Count (30ºC)
8.00
7.50
7.00
6.50
Log₁₀ cfu g⁻¹
Participants'
6.30 6.27
Participants' Median
Median -3SD
+ 0.5 log10
6.00
Participants' Median 5.77
5.50
Participants' Median
Median -3SD
- 0.5 log10
Participants'
5.24 5.27
5.00
4.50
2098
2099
FEPTU QC
Median
2096
2095
2093
2072
2035
2019
1970
1954
1940
1938
1930
1810
1803
1793
1783
1744
1664
1654
1636
1621
1609
1601
1507
1502
1456
1427
800
1301
785
784
735
724
700
506
502
497
452
445
415
325
318
316
288
279
243
226
166
18
127
4.23
Laboratory identification number
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
NP0144 - Aerobic Colony Count (22ºC)
8.00
7.50
7.00
6.50
Log₁₀ cfu g⁻¹
Participants' Median -3SD
+ 0.5 log10
6.27 6.27
6.00
Participants' Median 5.77
5.50
Participants' Median -+0.5
0.5log10
log10 5.27
5.27
5.00
4.50
FEPTU QC
Median
2099
2093
2019
1970
1810
1783
1654
1601
1507
1502
800
785
506
502
497
452
415
316
226
18
4.30
Laboratory identification number
Key: reported result by method
No Method Stated
Plate count agar
Petrifilm
MPN - Tempo
Other
The data in FEPTU reports is confidential
Page 21 of 23
Printed at 17:29 on Friday, 18 July, 2014
The data in FEPTU reports is confidential
Page 22 of 23
Printed at 17:29 on Friday, 18 July, 2014
The data in FEPTU reports is confidential
Page 23 of 23
Printed at 17:29 on Friday, 18 July, 2014

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