供試動物：CD (SD) IGS未成熟雌ラット(20日齢、6匹/群)
投与期間及び投与経路
・被験物質：0 (Vehicle)、100、300、1000 mg/kg/dayを3日間皮下投与
・EE (エチニルエストラジオール)： 0.6μg/kg/dayを3日間皮下投与
・TMX (タモキシフェン)：1 mg/kg/dayを3日間皮下投与
Chemicals
p-t -Butylphenol
Groups
(mg/kg/day)
Vehicle control
100
300
1,000
Vehicle + EE
100+EE
300+EE
1,000+EE
TMX+EE
Body weight (g)
62.2
63.4
63.2
61.2
63.7
62.7
62.2
60.3
62.7
±
±
±
±
±
±
±
±
±
3.6
4.2
5.5
5.4
3.0
3.6
5.0
4.4
4.3
Absolute Organ
Uterus wet wt.
32.9 ± 6.2
43.8 ± 7.0*
59.0 ± 9.2**
79.7 ± 13.7**
157.5 ± 39.4**
174.0 ± 20.7
124.9 ± 15.7
101.8 ± 29.4#
98.4 ± 10.8#
weight (mg)
Uterus blotted wt.
32.3 ± 6.1
43.3 ± 6.8*
58.1 ± 8.9**
78.6 ± 13.3**
121.9 ± 11.6**
129.3 ± 13.0
109.4 ± 8.9
95.2 ± 17.8#
97.1 ± 10.4##
Mean ± S.D.
* Significantly different from vehicle control at P<0.05, ** Significantly different from vehicle control at P<0.01
# Significantly different from vehicle control + EE at P<0.05, ## Significantly different from vehicle control + EE at P<0.01
Relative Organ weight (mg/100g)
Uterus wet wt.
Uterus blotted wt.
52.7 ± 8.2
51.8 ± 8.1
68.8 ± 7.8**
68.1 ± 7.6**
93.2 ± 10.9**
91.8 ± 10.6**
130.7 ± 21.5**
128.9 ± 21.3**
246.9 ± 57.1**
191.3 ± 13.0**
278.0 ± 33.3
206.2 ± 16.5
200.6 ± 18.4
175.9 ± 7.5#
166.9 ± 34.7#
156.9 ± 18.1##
156.9 ± 14.2#
154.9 ± 14.0##