䜰䝥䝸䜿䞊䝅䝵䞁䝕䞊䝍 䝋䝙䞊䠄ᰴ䠅 䝷䜲䝣䜶䝺䜽䝖䝻䝙䜽䝇ᴗᐊ Super Hybrid 検出系 細胞体積 検出器 前方散乱光 検出器 側方散乱光 検出器 蛍光検出器 2 • • • • 蛍光タンパク解析 セルサイクル＆核体積解析 アポトーシス＆細胞体積解析 アポトーシス解析 Fluorescence concentration (FC) on a per cell volume basis • • • • • 生菌・死菌数測定 Escherichia coli Viability DNA解析(ダブレット除去）DNA Analysis 細胞周期解析 Cell Cycle Analysis 倍数体解析 Plant Ploidy Analysis 免疫蛍光 Immunophenotyping – 1レーザー5カラー表面マーカー測定 – 2レーザー5カラー表面マーカー測定 – 3レーザー5カラー表面マーカー測定 蛍光タンパク解析 Fluorescent Proteins YFP HcRed レーザー：405nm 488nm 561nm GFP DsRed Mixture of Cells transfected with GFP, YFP, HcRed or DsRed. The sample was detected on an Eclipse with 488 nm, 405 nm and 561 nm lasers セルサイクル＆核体積解析 G0/G1 HPCV: 1.77% EV レーザー：405nm DAPI DAPI Region FL mean MCV Diameter (µm) CV Percentage Concentration (per mL) G0/G1 201.32 83.89 5.41 2.70% 52.92% 208,000 S 293.92 118.85 6.11 16.70% 35.43% 145,000 G2/M 386.85 140.17 6.48 1.75% 9.33% 31,000 EVを用いて、核体積（MCV)を測定 Jurkat cells were stained with DAPI Prep – DNA Staining Solution (PN: AE700570) then analyzed on an Eclipse with 405 nm lasers. アポトーシス＆細胞体積解析 レーザー：488nm Control Treated FS FS Apoptotic cells Anti-Active Caspase 3 - PE Anti-Active Caspase 3 - PE EV EV Apoptotic cells Anti-Active Caspase 3 - PE Anti-Active Caspase 3 - PE EVを用いて、アポトーシス細胞を明瞭に識別 EL4 mouse Lymphoma cells treated with (Treated) or without (Control)10 µM camptothecin for 16 hours. Cells were stained with Anti-active caspase 3-PE and analyzed on an Eclipse with a 488 nm laser. アポトーシス解析 Fluorescence concentration (FC) on a per cell volume basis レーザー：488nm No clear separation between + and - cell populations Anti-Active Caspase 3-PE Clear separation between + and populations with cell volume normalization FC (Anti-Active Caspase 3-PE) EL4 mouse Lymphoma cells treated with 10 µM camptothecin for 16 hours. Cells were stained with Anti-active caspase 3-PE and analyzed on an Eclipse with a 488 nm laser. 生菌・死菌数測定 Escherichia coli Viability レーザー：488nm PI Live: 88% PI Live: 42% SYTO 9 Control Live: 0.00% SYTO 9 PI Mixture of control and 70% ethanol treated samples E.Coli samples were stained with SYTO* 9 and propidium iodide (PI) then analyzed on an Eclipse with a 488 nm laser. SYTO 9 Treated with 70% ethanol *Registered trademarks of Life Technologies Corporation. DNA解析(ダブレット除去）DNA Analysis • Calf Thymocyte Nuclei (CTN Ref - DNA, PN: AE700585) stained with DAPI Prep – DNA Staining Solution (PN: AE700570) Detected on an Eclipse with 488 and 405 nm lasers レーザー：405nm Gate on singlet population HPCV: 1.79% CV: 2.86% DAPI (Peak) • DAPI (Integral) DAPI (Integral) 9 細胞周期解析 Cell Cycle Analysis レーザー：405nm EV G0/G1 HPCV: 1.77% DAPI DAPI Region FL mean MCV Diameter (µm) CV Percentage Concentration (per mL) G0/G1 201.32 83.89 5.41 2.70% 52.92% 208,000 S 293.92 118.85 6.11 16.70% 35.43% 145,000 G2/M 386.85 140.17 6.48 1.75% 9.33% 31,000 Jurkat cells were stained with DAPI Prep – DNA Staining Solution (PN: AE700570) then analyzed on an Eclipse with 488 nm and 405 nm lasers. 倍数体解析 Plant Ploidy Analysis レーザー：405nm Petunia The sample was stained with DAPI Prep – DNA Staining Solution (PN: AE700570) then analyzed on an Eclipse with 488 nm and 405 nm lasers. DAPI Region FL mean FL HPCV FL CV Diameter (µm) Percentage 2C 201.79 2.30% 3.71% 12.41 77.27% 1レーザー5カラー表面マーカー測定 FITC, PE, PETR, PE-Cy5, PE-Cy7 CD19-PETR CD16/56-PE CD8-FITC CD4-PECy7 レーザー：488 nm CD3-PECy5 CD3-PECy5 2レーザー5カラー表面マーカー測定 FITC, PE, APC, Alexa700, PE-Cy7 CD19-APC CD16/56-PE CD4-PECy7 CD8-Alexa* 700 レーザー：488 nm , 642nm CD3-FITC CD3-FITC *Registered trademarks of Life Technologies Corporation. 3レーザー5カラー表面マーカー測定 Pacific Blue, FITC, PE, APC, PE-Cy7 CD19-APC CD16/56-PE CD4-PECy7 CD8-PacBlue* レーザー： 405 nm, 488 nm, 642 nm CD3-FITC CD3-FITC *Registered trademarks of Life Technologies Corporation.
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