ANTIBIOTICS
ECLIPSE
Antibiotics are used for prevention and treatment of animals having bacterial infections of the udder (mastitis).
Milk is examined for antibiotics residues because they pose a hazard to consumers and could cause production
problems of fermented milk products. The detection of antibiotics is compulsory and levels should comply with
the Maximum Residue Limits (MRL) state by the European Union (Commission Regulation (EU) No 37/2010).
Eclipse is a microbial growth inhibition test that can be used for detection of antibiotic residues in milk. It is
very simple and ready-to-use test, with a sensitivity complying with EU-MRLs.
Qualitative detection of inhibitory substances in cow, sheep and goat milk
Easy:
one-step assay
Sensitive:
complying with EU MRLs
Qualitative:
broad-range screening of inhibitors
Assay time:
about 2h 30min
Reading:
visual or photometrical (590 and 650 nm)
Suitable for:
cow, sheep, goat and buffalo milk
Format:
individual tests in plate and tube format
ECLIPSE 3G. ECLIPSE FARM 3G.
Assay procedure
Negative
2.30
65
Positive
1
Add 100 µl of milk
2
Incubate at 65ºC ≈ 2.30 h
3 Result
ZEU—INMUNOTEC, S.L. C/Bari, 25 dpdo. 50197 Zaragoza (SPAIN) +(34) 976 731 533
[email protected] www.zeulab.com
INHIBITOR
Eclipse (µg/L)
EU-M RL (µg/ L)
β-LACTAMS
Amoxicillin
3
4
Ampicillin
3
4
Cefalexin
60
100
Cefapirin
8
60
Desacetylcefapirin
<40
60
Evaluated by ILVO (The Institute
for Agricultural
and Fisheries Research)
from Belgium
Cefapirin Benzathin
<40
Cefalonium
20
20
Ceftiofur
60
100
Cefazolin
20
50
Complying with criteria stipulated
by Belgium
Food Safety Agency
(AFSCA)
Cefquinome
125
20
Cloxacillin
30
30
Oxacillin
10
30
Nafcilin
<20
30
Penicillin G
2-3
4
Doxycycline
100
-
Oxytetracycline
100
100
Tetracycline
100
100
Sulfadiazine
100
100
Sulfametazine
150
100
Sulfamethoxypyridazine
100
100
Sulfathiazole
100
100
Erythromycin
200
40
Tylosin
40
50
Streptomycin
1.500
200
Gentamcin
> 1000
100
Neomycin
1.500
1500
Kanamycin
>2.000
150
Spectinomycin
>2.500
200
150
150
Chloramphenicol
5.000
-
Bacitracin
600
100
>1.000
50
Official test in France
ECLIPSE
TETRACYCLINES
SULFONAM IDES
M ACROLIDES
AMINOGLYCOSIDES
LINCOSAMIDES
Lincomycin
OTHERS
Colistin
ZEU—INMUNOTEC, S.L. C/Bari, 25 dpdo. 50197 Zaragoza (SPAIN) +(34) 976 731 533
[email protected] www.zeulab.com

Angeliki Tsigouri - European Medicines Agency

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